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The map label for this gene is nucA [H]

Identifier: 37521867

GI number: 37521867

Start: 2462385

End: 2463428

Strand: Direct

Name: nucA [H]

Synonym: glr2298

Alternate gene names: 37521867

Gene position: 2462385-2463428 (Clockwise)

Preceding gene: 37521866

Following gene: 37521871

Centisome position: 52.85

GC content: 61.4

Gene sequence:

>1044_bases
ATGCGCCCGTGGCACAATCGACTCTCAATAGCGATTGTGCCGATGAGCAAAGCTCCCCCCCGTACCGTTCTGCTGGGCAT
TGTTCTACTGACCGTCTCCGTCGGTTCGTGCGCCCCGGCTAAGAAGCAGCGCTTCCGCTGCGCCGACTTTACTTTGCCCC
AGCAGGTGATCGACGCCTACCGCGGCGGTGCCACTTACCTCGACAACGACGGCAATGGCTACGCCTGCGAGGACAAATTC
GATGTCTACGTGGGCAAGGGCAAGCCCAAAAACCGGGACAACATCGCCGCCCTTCCCCCTGCAGACAAGGCTGGCACCGC
TGCAGAGCAGCTGCTGTTAGGCAACCCGAGCCGGGCGACAGCCACCCCCGGCAGTGTCAGTAACTATCTGCTTCTCAAGC
CGCAGTACGCGCTTTCGTACAACGCGGCCAAAGGGATTCCCAACTGGGCCAGCTGGCAACTCAACCGCTCCTGGCTGGGA
AGGACCGATCGCCAGAACGACTTTCGCCCGGATGAGAGCCTGCCGGTAAGTTTTTACCGGGTAAATCCCGCCGACTACAC
TGGCAGCGGCTACGACCGGGGCCACCTGGTGCCCTCGGCGGACCGCACGGTAACTGCGGCGGACAACTCGGCGACTTTTT
TGATGACCAATATGACTCCTCAGGCCCCGGATCTCAACCGCGGCCCCTGGGAAAAACTCGAAAGCTACTGCCGCGAACTG
GTCGCGGCGGGGAAGGAACTTTACATCGTGGCCGGGGGTAGCGGGGAACTTGGGAAAATCGGCGGGGGCAAAATATCTGT
TCCCGCGCGCCACTGGAAGGTGATCGTCGTGCTCGATGCGCCGGTGACAAGCCTCGCAAGTGTGACCGGACGCACGCGGG
TGATCGCCGTGGACATGCCCAACACCTCCGGGATCAAAGAAGTGGGCTGGCGCGAATTTCGGGTGAATGTCGACCGCATC
GAAGCGGCCACCGGCTACAACCTACTCTCGGGCGTGCCAAAGAACATCCAGGCGTTACTCGAAAGCCGCTTGGACAATCG
GTAA

Upstream 100 bases:

>100_bases
CGCCCATGCCCTGAAGGTGCTGGGCCGCTAGTCGACGAGTACTGCCGATCGACCGGGGGAGTGCAGGCCACTCCCCCGGT
TTGTTTTTGCAGCCTGTTGG

Downstream 100 bases:

>100_bases
GTTTTCAGTTGCACAGGCAACGCCAGGCGAGCGGGAAACCCTTGCGATCAATCGTTTTCACCCTGACACCCGGCACCTGC
TCTAAGTTTCGACGATCCGG

Product: sugar-non-specific nuclease NucA-like protein

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRPWHNRLSIAIVPMSKAPPRTVLLGIVLLTVSVGSCAPAKKQRFRCADFTLPQQVIDAYRGGATYLDNDGNGYACEDKF
DVYVGKGKPKNRDNIAALPPADKAGTAAEQLLLGNPSRATATPGSVSNYLLLKPQYALSYNAAKGIPNWASWQLNRSWLG
RTDRQNDFRPDESLPVSFYRVNPADYTGSGYDRGHLVPSADRTVTAADNSATFLMTNMTPQAPDLNRGPWEKLESYCREL
VAAGKELYIVAGGSGELGKIGGGKISVPARHWKVIVVLDAPVTSLASVTGRTRVIAVDMPNTSGIKEVGWREFRVNVDRI
EAATGYNLLSGVPKNIQALLESRLDNR

Sequences:

>Translated_347_residues
MRPWHNRLSIAIVPMSKAPPRTVLLGIVLLTVSVGSCAPAKKQRFRCADFTLPQQVIDAYRGGATYLDNDGNGYACEDKF
DVYVGKGKPKNRDNIAALPPADKAGTAAEQLLLGNPSRATATPGSVSNYLLLKPQYALSYNAAKGIPNWASWQLNRSWLG
RTDRQNDFRPDESLPVSFYRVNPADYTGSGYDRGHLVPSADRTVTAADNSATFLMTNMTPQAPDLNRGPWEKLESYCREL
VAAGKELYIVAGGSGELGKIGGGKISVPARHWKVIVVLDAPVTSLASVTGRTRVIAVDMPNTSGIKEVGWREFRVNVDRI
EAATGYNLLSGVPKNIQALLESRLDNR
>Mature_347_residues
MRPWHNRLSIAIVPMSKAPPRTVLLGIVLLTVSVGSCAPAKKQRFRCADFTLPQQVIDAYRGGATYLDNDGNGYACEDKF
DVYVGKGKPKNRDNIAALPPADKAGTAAEQLLLGNPSRATATPGSVSNYLLLKPQYALSYNAAKGIPNWASWQLNRSWLG
RTDRQNDFRPDESLPVSFYRVNPADYTGSGYDRGHLVPSADRTVTAADNSATFLMTNMTPQAPDLNRGPWEKLESYCREL
VAAGKELYIVAGGSGELGKIGGGKISVPARHWKVIVVLDAPVTSLASVTGRTRVIAVDMPNTSGIKEVGWREFRVNVDRI
EAATGYNLLSGVPKNIQALLESRLDNR

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=204, Percent_Identity=30.8823529411765, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI53759134, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI224451075, Length=179, Percent_Identity=30.1675977653631, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17505967, Length=219, Percent_Identity=27.3972602739726, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322253, Length=229, Percent_Identity=32.3144104803493, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129899, Length=183, Percent_Identity=30.0546448087432, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24581065, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: NA

Molecular weight: Translated: 37730; Mature: 37730

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPWHNRLSIAIVPMSKAPPRTVLLGIVLLTVSVGSCAPAKKQRFRCADFTLPQQVIDAY
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCEEECCCCCHHHHHHHH
RGGATYLDNDGNGYACEDKFDVYVGKGKPKNRDNIAALPPADKAGTAAEQLLLGNPSRAT
CCCCEEEECCCCCEEECCCEEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCC
ATPGSVSNYLLLKPQYALSYNAAKGIPNWASWQLNRSWLGRTDRQNDFRPDESLPVSFYR
CCCCCCCCEEEECCCHHEEECHHCCCCCHHCEEECHHHCCCCCCCCCCCCCCCCCEEEEE
VNPADYTGSGYDRGHLVPSADRTVTAADNSATFLMTNMTPQAPDLNRGPWEKLESYCREL
ECCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
VAAGKELYIVAGGSGELGKIGGGKISVPARHWKVIVVLDAPVTSLASVTGRTRVIAVDMP
HHCCCEEEEEECCCCCCCCCCCCEEECCCCCEEEEEEECCCHHHHHHCCCCEEEEEEECC
NTSGIKEVGWREFRVNVDRIEAATGYNLLSGVPKNIQALLESRLDNR
CCCCHHHCCCEEEEEEHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPWHNRLSIAIVPMSKAPPRTVLLGIVLLTVSVGSCAPAKKQRFRCADFTLPQQVIDAY
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCEEECCCCCHHHHHHHH
RGGATYLDNDGNGYACEDKFDVYVGKGKPKNRDNIAALPPADKAGTAAEQLLLGNPSRAT
CCCCEEEECCCCCEEECCCEEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCC
ATPGSVSNYLLLKPQYALSYNAAKGIPNWASWQLNRSWLGRTDRQNDFRPDESLPVSFYR
CCCCCCCCEEEECCCHHEEECHHCCCCCHHCEEECHHHCCCCCCCCCCCCCCCCCEEEEE
VNPADYTGSGYDRGHLVPSADRTVTAADNSATFLMTNMTPQAPDLNRGPWEKLESYCREL
ECCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
VAAGKELYIVAGGSGELGKIGGGKISVPARHWKVIVVLDAPVTSLASVTGRTRVIAVDMP
HHCCCEEEEEECCCCCCCCCCCCEEECCCCCEEEEEEECCCHHHHHHCCCCEEEEEEECC
NTSGIKEVGWREFRVNVDRIEAATGYNLLSGVPKNIQALLESRLDNR
CCCCHHHCCCEEEEEEHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]