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The map label for this gene is eno

Identifier: 37521690

GI number: 37521690

Start: 2275146

End: 2276423

Strand: Reverse

Name: eno

Synonym: gll2121

Alternate gene names: 37521690

Gene position: 2276423-2275146 (Counterclockwise)

Preceding gene: 37521691

Following gene: 37521686

Centisome position: 48.86

GC content: 59.47

Gene sequence:

>1278_bases
ATGCTGATTACATCGATCGAAGCGCACGAAATTCTCGATTCACGGGGCAATCCGACCGTCGAAGCACAGGTGGCGCTTGA
AAACGGGACGACCGGCTCGGCCATCGTTCCTTCGGGCGCCTCCACCGGCGAAAAAGAAGCGGTGGAGTTGCGCGACAGCG
ATCCGAAGCGCTACGGCGGCAAAGGGGTGCTGAGCGCCGTGGCCAACGTCAACGACTCGATCGCCCCCGAAATTATCGGC
ATGGAAGTCACCGACCAGCGCAGCATCGACCGCAAGATGATCGAAATCGACGGTACCGCCAACAAAGCCACCCTCGGCGC
CAACGCGATCTTGGCGGTCTCGATGGCGGTGGCACGCACCGCCGCTCTCTCGCTGGGGCTGCCTCTCTATCGCTATTTGG
GCGGTACGAACGCCTCGCTGTTGCCGGTACCCTGCATGAACGTGATCAACGGCGGCAAGCACGCCGACAACACCGTCGAT
TTTCAAGAATTTATGATCGCCCCGCACAACGCACCCAATTTTGCCGAGGCACTACGCATGGGAGCTGAGACTTTCCATGC
CCTCAAATCGATCCTGCGCGGCAAAGGCTACAGCACGGGCGTGGGCGACGAAGGCGGCTTTGCCCCCGATCTCAAGTCCA
ACGAAGAGGCGGTCGAAGTGATTTTGCTTGCGATCGAAAAGGCCGGCTACCGGCCCGGCGAGGACATCTCGATCTGTCTT
GACCCGGCCACCAGCGAGATGTGGAAGGACGGCAAGTACTTGTTCTTCAAGTCCGACCAGTCCACCAAGACCTCCGAGGA
AATGGTCGATCTGTGGGCTTCCTGGGCAGGCCAGTATCCGATCGTTTCGCTCGAAGACGGCATGGGTGAGAACGATTGGG
AAGGCTGGAAAATGCTCACCGACCGCATCGGTGCCACTGTCGAGCTGGTAGGAGACGACCTGTTTTGCACTAACGCTAAA
ATTCTTGCCGAAGGCATCGAAAAGGGTGTCGCCAATTCCATTTTGATCAAATTGAATCAGATCGGTTCGGTCAGCGAGAC
GCTCGACACCATCGAACTGGCCGTCAAGCACAACTACAAATGTTTTGTCTCCCATCGCTCCGGCGAGACCGAGGACACGA
CCATTGCGGATCTGACCGTGGCGACCGCCGCGGGCCAGATCAAGACCGGCTCCGGCTGCCGCAGTGAGCGGGTGGCCAAG
TTCAACCAGTTACTGAGAATCGAGCGCCAGCTCGGCGAGGCGGCCCGCTTCGCGGGCAAGTCCGCCTTTGCGCGTTAG

Upstream 100 bases:

>100_bases
CCAACTGTTGATTACCTGGCGCTTTTTGGAGCAAATCAACTATTTGCTTCAAAAAAACCGATAGTGCACTCTTAATCTTT
TAAGACAAGGAGTTCTTGGC

Downstream 100 bases:

>100_bases
GCGATTTCCAGGTGTCTGTCGCATGCAGCCGCCCGTCTGCTCCGCCACCGCTTGCGCACTGCCGAAGTACCCCCAACGCG
GCCCACCGGCCAAACCACCG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLITSIEAHEILDSRGNPTVEAQVALENGTTGSAIVPSGASTGEKEAVELRDSDPKRYGGKGVLSAVANVNDSIAPEIIG
MEVTDQRSIDRKMIEIDGTANKATLGANAILAVSMAVARTAALSLGLPLYRYLGGTNASLLPVPCMNVINGGKHADNTVD
FQEFMIAPHNAPNFAEALRMGAETFHALKSILRGKGYSTGVGDEGGFAPDLKSNEEAVEVILLAIEKAGYRPGEDISICL
DPATSEMWKDGKYLFFKSDQSTKTSEEMVDLWASWAGQYPIVSLEDGMGENDWEGWKMLTDRIGATVELVGDDLFCTNAK
ILAEGIEKGVANSILIKLNQIGSVSETLDTIELAVKHNYKCFVSHRSGETEDTTIADLTVATAAGQIKTGSGCRSERVAK
FNQLLRIERQLGEAARFAGKSAFAR

Sequences:

>Translated_425_residues
MLITSIEAHEILDSRGNPTVEAQVALENGTTGSAIVPSGASTGEKEAVELRDSDPKRYGGKGVLSAVANVNDSIAPEIIG
MEVTDQRSIDRKMIEIDGTANKATLGANAILAVSMAVARTAALSLGLPLYRYLGGTNASLLPVPCMNVINGGKHADNTVD
FQEFMIAPHNAPNFAEALRMGAETFHALKSILRGKGYSTGVGDEGGFAPDLKSNEEAVEVILLAIEKAGYRPGEDISICL
DPATSEMWKDGKYLFFKSDQSTKTSEEMVDLWASWAGQYPIVSLEDGMGENDWEGWKMLTDRIGATVELVGDDLFCTNAK
ILAEGIEKGVANSILIKLNQIGSVSETLDTIELAVKHNYKCFVSHRSGETEDTTIADLTVATAAGQIKTGSGCRSERVAK
FNQLLRIERQLGEAARFAGKSAFAR
>Mature_425_residues
MLITSIEAHEILDSRGNPTVEAQVALENGTTGSAIVPSGASTGEKEAVELRDSDPKRYGGKGVLSAVANVNDSIAPEIIG
MEVTDQRSIDRKMIEIDGTANKATLGANAILAVSMAVARTAALSLGLPLYRYLGGTNASLLPVPCMNVINGGKHADNTVD
FQEFMIAPHNAPNFAEALRMGAETFHALKSILRGKGYSTGVGDEGGFAPDLKSNEEAVEVILLAIEKAGYRPGEDISICL
DPATSEMWKDGKYLFFKSDQSTKTSEEMVDLWASWAGQYPIVSLEDGMGENDWEGWKMLTDRIGATVELVGDDLFCTNAK
ILAEGIEKGVANSILIKLNQIGSVSETLDTIELAVKHNYKCFVSHRSGETEDTTIADLTVATAAGQIKTGSGCRSERVAK
FNQLLRIERQLGEAARFAGKSAFAR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=50.6944444444444, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=50.2314814814815, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=49.8839907192575, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=49.8839907192575, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=45.8139534883721, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=25.5131964809384, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=25.5131964809384, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=25.5131964809384, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1789141, Length=422, Percent_Identity=56.8720379146919, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=52.4137931034483, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=52.4137931034483, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=51.0416666666667, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=435, Percent_Identity=49.8850574712644, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=47.5750577367206, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=47.5750577367206, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=47.3441108545035, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321968, Length=435, Percent_Identity=48.735632183908, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=50.2304147465438, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=50.2304147465438, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=50.2304147465438, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=50.2304147465438, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=50.2304147465438, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=50.2304147465438, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_GLOVI (Q7NIR1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925067.1
- ProteinModelPortal:   Q7NIR1
- SMR:   Q7NIR1
- GeneID:   2600522
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll2121
- NMPDR:   fig|251221.1.peg.2121
- HOGENOM:   HBG726599
- OMA:   CMNVING
- ProtClustDB:   PRK00077
- BioCyc:   GVIO251221:GLL2121-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45453; Mature: 45453

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00164 ENOLASE; PS00290 IG_MHC

Important sites: ACT_SITE 204-204 ACT_SITE 337-337 BINDING 154-154 BINDING 163-163 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITSIEAHEILDSRGNPTVEAQVALENGTTGSAIVPSGASTGEKEAVELRDSDPKRYGG
CEEECCHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCCEEECCCCCCHHCCC
KGVLSAVANVNDSIAPEIIGMEVTDQRSIDRKMIEIDGTANKATLGANAILAVSMAVART
HHHHHHHHCCCCCCCCCEECEEECCCCCCCCEEEEECCCCCCEECCCHHHHHHHHHHHHH
AALSLGLPLYRYLGGTNASLLPVPCMNVINGGKHADNTVDFQEFMIAPHNAPNFAEALRM
HHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHEECCCCCCCHHHHHHH
GAETFHALKSILRGKGYSTGVGDEGGFAPDLKSNEEAVEVILLAIEKAGYRPGEDISICL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
DPATSEMWKDGKYLFFKSDQSTKTSEEMVDLWASWAGQYPIVSLEDGMGENDWEGWKMLT
CCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHH
DRIGATVELVGDDLFCTNAKILAEGIEKGVANSILIKLNQIGSVSETLDTIELAVKHNYK
HHHCCEEEEECCCEEECCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCE
CFVSHRSGETEDTTIADLTVATAAGQIKTGSGCRSERVAKFNQLLRIERQLGEAARFAGK
EEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
SAFAR
HHCCC
>Mature Secondary Structure
MLITSIEAHEILDSRGNPTVEAQVALENGTTGSAIVPSGASTGEKEAVELRDSDPKRYGG
CEEECCHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCCEEECCCCCCHHCCC
KGVLSAVANVNDSIAPEIIGMEVTDQRSIDRKMIEIDGTANKATLGANAILAVSMAVART
HHHHHHHHCCCCCCCCCEECEEECCCCCCCCEEEEECCCCCCEECCCHHHHHHHHHHHHH
AALSLGLPLYRYLGGTNASLLPVPCMNVINGGKHADNTVDFQEFMIAPHNAPNFAEALRM
HHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHEECCCCCCCHHHHHHH
GAETFHALKSILRGKGYSTGVGDEGGFAPDLKSNEEAVEVILLAIEKAGYRPGEDISICL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
DPATSEMWKDGKYLFFKSDQSTKTSEEMVDLWASWAGQYPIVSLEDGMGENDWEGWKMLT
CCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHH
DRIGATVELVGDDLFCTNAKILAEGIEKGVANSILIKLNQIGSVSETLDTIELAVKHNYK
HHHCCEEEEECCCEEECCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCE
CFVSHRSGETEDTTIADLTVATAAGQIKTGSGCRSERVAKFNQLLRIERQLGEAARFAGK
EEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
SAFAR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292