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The map label for this gene is purF [H]

Identifier: 37521683

GI number: 37521683

Start: 2266914

End: 2268350

Strand: Direct

Name: purF [H]

Synonym: gvip291

Alternate gene names: 37521683

Gene position: 2266914-2268350 (Clockwise)

Preceding gene: 37521682

Following gene: 37521685

Centisome position: 48.66

GC content: 64.79

Gene sequence:

>1437_bases
ATGACCTACGCTGCACCCTCTGCTCCCGACAAGCCTGAGGAGGCTTGCGGGGTCTTCGGCATCCTGGCGCCGGGCGAAGC
CGTCGCCAAATTGACCTACTTCGGTCTGTTCGCGCTCCAGCATCGCGGCCAGGAGTCCGCGGGGATCGCTGTGCTCGACG
GCGACCGTCTAACGATGCACAAGGACATGGGTCTGGTCTCCCAGGTCTTTGACGAGGCACTGCTAAACGAGCTGCAGGGG
CAATTGGCCGTGGGCCACACCCGCTACTCGACCACCGGCTCCAGCCGCAAGGTAAACGCCCAGCCGGTCGTCTCCCGCAC
GCGCCTCGGTCCGGTCGTCCTCGCCCACAACGGCAACCTGGTCAACGCCGCCCAGTTGCGCGAAGAGTTGCTCGCCCGCG
AGCACACCCTGCTGGCCACCACCGATTCCGAAGAGATTGTCCACGCCATCGGCGAGGCGGTCGACGACGGCAAGGGTTGG
GTCGAGGGGACCGTTCACGCCCTCAAGCGCTGCCGCGGCGCCTTCAGCCTGGTGATCGGCACCCCGGCGGGGCTGATGGG
CACCCGCGACGCCAACGGCGTGCGTCCGCTGGTCATCGGCACCCTCAAGGGCCGCTACGTGCTCGCGAGTGAGACCTGCG
CCCTCAGCATCATCGGTGCGAAATACCTGCGCGACGTCGAGCCCGGCGAACTGGTGTTCATCACCGAATCGGGCCTGGAG
TCCCACCACTGGGCGAAACCCGAACCGCGGATGTGCATCTTTGAGATGATCTATTTTGCCCGCCCCGATTCGCGCGTGAA
CGCCGAGAGCCTCTACACCTACCGCGAGCGCCTGGGCGAGATCCTCGCTCAGGAGGCCCCTGTGGAGGCGGACGTCGTCA
TCCCGGTCCCCGACTCGGGTACCCCCGCCGCCATCGGCTACGCCCGCGCCTCCGGCATTCTCTTCCAGCAAGGTCTCATC
AAGAATCACTACGTAGGCCGCACGTTCATCCAACCCACCCAGACGATGCGCGAGGCGGGCATCAAGATGAAGCTCAATCC
GTTGCCCGACGTCATCGAAGGCAAGCGGATCGTAATCGTCGACGATTCGATCGTGCGCGGCACCACCAGCCGCAAGATTG
TCCAGGCCCTGCGCGACAGCGGTGCGCGCGAGGTGCACATGCGTATCTCCTCGCCCCCGGTCACCCATCCCTGTTTCTAC
GGCATCGACACCGACTCGCAGGACCAGTTGATTGCCGCCACCAAGAGCGTGGATGAAATTGCCCGGCACATCGAGGTGGA
CAGCCTTGCCTACTTGAGTGTCGAAGGGATGCTCAAGGCGACCCGCACCGACGGCCAGGGCTTTTGCACCGCCTGCTTCA
CCGGCGATTACCCGATTCCGATTCCTGATGCGATCCGCCGCACCAAACTGGTGCTCGAAAAAACCGCAGCCAGCTGA

Upstream 100 bases:

>100_bases
ATTGCCGCATCTCCACTTCCTGCGAGCGGCTCAAGACCACCTTCGAACAAGCCATCCCCCGCCGGATGGAGCACATACCT
GTCACCGAGGCACCCCAACG

Downstream 100 bases:

>100_bases
TCCCGATTCCAGAGCCTCGGCTGAGCGACATGCCGCTACTGCAGCGGGTGTTTCACCCCGGATGCCCAGACCCAGGCTCG
GCACGGGAAAACTCAAAATT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTYAAPSAPDKPEEACGVFGILAPGEAVAKLTYFGLFALQHRGQESAGIAVLDGDRLTMHKDMGLVSQVFDEALLNELQG
QLAVGHTRYSTTGSSRKVNAQPVVSRTRLGPVVLAHNGNLVNAAQLREELLAREHTLLATTDSEEIVHAIGEAVDDGKGW
VEGTVHALKRCRGAFSLVIGTPAGLMGTRDANGVRPLVIGTLKGRYVLASETCALSIIGAKYLRDVEPGELVFITESGLE
SHHWAKPEPRMCIFEMIYFARPDSRVNAESLYTYRERLGEILAQEAPVEADVVIPVPDSGTPAAIGYARASGILFQQGLI
KNHYVGRTFIQPTQTMREAGIKMKLNPLPDVIEGKRIVIVDDSIVRGTTSRKIVQALRDSGAREVHMRISSPPVTHPCFY
GIDTDSQDQLIAATKSVDEIARHIEVDSLAYLSVEGMLKATRTDGQGFCTACFTGDYPIPIPDAIRRTKLVLEKTAAS

Sequences:

>Translated_478_residues
MTYAAPSAPDKPEEACGVFGILAPGEAVAKLTYFGLFALQHRGQESAGIAVLDGDRLTMHKDMGLVSQVFDEALLNELQG
QLAVGHTRYSTTGSSRKVNAQPVVSRTRLGPVVLAHNGNLVNAAQLREELLAREHTLLATTDSEEIVHAIGEAVDDGKGW
VEGTVHALKRCRGAFSLVIGTPAGLMGTRDANGVRPLVIGTLKGRYVLASETCALSIIGAKYLRDVEPGELVFITESGLE
SHHWAKPEPRMCIFEMIYFARPDSRVNAESLYTYRERLGEILAQEAPVEADVVIPVPDSGTPAAIGYARASGILFQQGLI
KNHYVGRTFIQPTQTMREAGIKMKLNPLPDVIEGKRIVIVDDSIVRGTTSRKIVQALRDSGAREVHMRISSPPVTHPCFY
GIDTDSQDQLIAATKSVDEIARHIEVDSLAYLSVEGMLKATRTDGQGFCTACFTGDYPIPIPDAIRRTKLVLEKTAAS
>Mature_477_residues
TYAAPSAPDKPEEACGVFGILAPGEAVAKLTYFGLFALQHRGQESAGIAVLDGDRLTMHKDMGLVSQVFDEALLNELQGQ
LAVGHTRYSTTGSSRKVNAQPVVSRTRLGPVVLAHNGNLVNAAQLREELLAREHTLLATTDSEEIVHAIGEAVDDGKGWV
EGTVHALKRCRGAFSLVIGTPAGLMGTRDANGVRPLVIGTLKGRYVLASETCALSIIGAKYLRDVEPGELVFITESGLES
HHWAKPEPRMCIFEMIYFARPDSRVNAESLYTYRERLGEILAQEAPVEADVVIPVPDSGTPAAIGYARASGILFQQGLIK
NHYVGRTFIQPTQTMREAGIKMKLNPLPDVIEGKRIVIVDDSIVRGTTSRKIVQALRDSGAREVHMRISSPPVTHPCFYG
IDTDSQDQLIAATKSVDEIARHIEVDSLAYLSVEGMLKATRTDGQGFCTACFTGDYPIPIPDAIRRTKLVLEKTAAS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=41.025641025641, Blast_Score=359, Evalue=4e-99,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=39.0191897654584, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=34.375, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=42.4116424116424, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=36.231884057971, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=44.6028513238289, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=42.0081967213115, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=42.5806451612903, Blast_Score=370, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51767; Mature: 51636

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYAAPSAPDKPEEACGVFGILAPGEAVAKLTYFGLFALQHRGQESAGIAVLDGDRLTMH
CCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEE
KDMGLVSQVFDEALLNELQGQLAVGHTRYSTTGSSRKVNAQPVVSRTRLGPVVLAHNGNL
HHHHHHHHHHHHHHHHHHCCCEEECEEEEECCCCCCCCCCCCCHHHHCCCCEEEECCCCE
VNAAQLREELLAREHTLLATTDSEEIVHAIGEAVDDGKGWVEGTVHALKRCRGAFSLVIG
ECHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEE
TPAGLMGTRDANGVRPLVIGTLKGRYVLASETCALSIIGAKYLRDVEPGELVFITESGLE
CCCCCCCCCCCCCCCEEEEEEECCCEEEEECCEEEEEHHHHHHCCCCCCCEEEEECCCCC
SHHWAKPEPRMCIFEMIYFARPDSRVNAESLYTYRERLGEILAQEAPVEADVVIPVPDSG
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
TPAAIGYARASGILFQQGLIKNHYVGRTFIQPTQTMREAGIKMKLNPLPDVIEGKRIVIV
CCCEEEHHHHCCHHHHHCCHHCCCCCCEECCHHHHHHHCCCEEEECCCCHHHCCCEEEEE
DDSIVRGTTSRKIVQALRDSGAREVHMRISSPPVTHPCFYGIDTDSQDQLIAATKSVDEI
CCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
ARHIEVDSLAYLSVEGMLKATRTDGQGFCTACFTGDYPIPIPDAIRRTKLVLEKTAAS
HHHCCCCCEEEEEHHHHHEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYAAPSAPDKPEEACGVFGILAPGEAVAKLTYFGLFALQHRGQESAGIAVLDGDRLTMH
CCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEE
KDMGLVSQVFDEALLNELQGQLAVGHTRYSTTGSSRKVNAQPVVSRTRLGPVVLAHNGNL
HHHHHHHHHHHHHHHHHHCCCEEECEEEEECCCCCCCCCCCCCHHHHCCCCEEEECCCCE
VNAAQLREELLAREHTLLATTDSEEIVHAIGEAVDDGKGWVEGTVHALKRCRGAFSLVIG
ECHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEE
TPAGLMGTRDANGVRPLVIGTLKGRYVLASETCALSIIGAKYLRDVEPGELVFITESGLE
CCCCCCCCCCCCCCCEEEEEEECCCEEEEECCEEEEEHHHHHHCCCCCCCEEEEECCCCC
SHHWAKPEPRMCIFEMIYFARPDSRVNAESLYTYRERLGEILAQEAPVEADVVIPVPDSG
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
TPAAIGYARASGILFQQGLIKNHYVGRTFIQPTQTMREAGIKMKLNPLPDVIEGKRIVIV
CCCEEEHHHHCCHHHHHCCHHCCCCCCEECCHHHHHHHCCCEEEECCCCHHHCCCEEEEE
DDSIVRGTTSRKIVQALRDSGAREVHMRISSPPVTHPCFYGIDTDSQDQLIAATKSVDEI
CCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
ARHIEVDSLAYLSVEGMLKATRTDGQGFCTACFTGDYPIPIPDAIRRTKLVLEKTAAS
HHHCCCCCEEEEEHHHHHEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]