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The map label for this gene is ndhF [H]

Identifier: 37521652

GI number: 37521652

Start: 2235433

End: 2237289

Strand: Reverse

Name: ndhF [H]

Synonym: gvip278

Alternate gene names: 37521652

Gene position: 2237289-2235433 (Counterclockwise)

Preceding gene: 37521653

Following gene: 37521651

Centisome position: 48.02

GC content: 62.09

Gene sequence:

>1857_bases
ATGACCGAATCGCTCCTTTTCACAAGCTGGTGGATACCCTTCTATGGGCTGATCGGCGCGGTGTTGACCCTGCCCTGGGC
GACGGTCGGGGTGCGTAGCGGCGGACCGCGGCCGGCGGCTTACTTCAACATTTTGATGGCGGTGCTGGCCCTAGTGCATG
GCCTGACGTTGCTGCCCGCCGTCTGGGGGCGACTGCCGCTGCAGCTGGAATACACCTGGCTTGAAACACAGGGAATGCGT
CTGGCCCTCGAATTGGAGGTCTCGCCGGTCACGATTGGCGCGGTGGTGGTCATTACGTTTTTGAGTTTGTGCGCCCAGAT
CTATGCCCTGGGTTACATGGAAAAGGACTGGGCGCTGGCGCGCTTCTTCGCGCTGTTGGGCTTTTTTGAAGGAGCGATCT
GCGGCCTGGCGATGAGCAACAACTTGCTGTTGAGCTACGCGCTGCTGGAGATGCTCACCCTTTCGACTTATCTGCTGGTG
GGCTTCTGGTACGCCCAGCCGCTGGTGGTGACCGCGGCGCGCGACGCCTTCCTGACCAAGCGGGTGGGGGATCTGTTGCT
GTTGATGGGGGTGGTGGTCCTGGCCAATCTCGCCCCCAGCCTCAATTTTCAAGACCTGGCGCTCTGGGCTGAGACGGCCG
ATTTGCCCCCCTTGAGCGCCACACTCCTGGGACTGGCCCTGATTGCCGGTCCAATCGGCAAGTGTGCCCAGATCCCGTTG
CACTTCTGGCTCGACGAGGCGATGGAAGGACCCAACCCCGCCTCGATCATGCGCAACTCGCTGGTAGTGGCCTGTGGAGC
CTACGTGCTCATCAAGATGCAGCCGGTTCTGGCGCTTTCGCCCTGGGTACTGGGAGCCTTGCTGGTGGTGGGCACAGTGA
CGGCGGTCGGCGCCTCCTGTGTAGCCCTGGCACAGATCGACATCAAGCGTGCTCTCTCCCATTCGACAAGTGCCTACCTG
GGCTTGGTGTTTGTCGCCGTGGGCCTCGAGCAAAACGAAGTGGCGCTGCTGCTGCTGCTGACCCACGCCGTCGCCAAGGC
GTTGCTGTTTATGAGTGTCGGGGCGGTGATCTTGATTACCAGCACCCAGGATGTCACCGAGATGGGCGGGTTGTGGTCGC
GCCTGCCGGCCACTACCACCGCCTTTGTGGTCGGAGGGGCCGCTTCGGTGTCGCTGCTGCCTCTGGGCACTTTTTGGGCT
TCGATCTACGCGAGCGAGCGGCTTTGGACCTGGGCTCCCTGGGCGGTCGGGGTGTTGCTGCTGGTCAACGGGCTCACCGC
TCTCAATCTCACCCGCGTTTACTGCCGGGTCTTCCTGGGGCCTCGACAGGCCAAGACCCGGCGCGCTCCCGAAATCGCCT
GGCCGATGTCGGTGCCGATGTTGTCGCTTTCGATCATCAACATCCTCTCGCCGGTGATTCTGCTCCAGTTGGGACTTTTG
ACTTCGGTGGAAGGACCGGTGCCCACCTGGGTGTTCGGCGGTTCGCTGATGGCTTCCAGCCTGGCGGGCTGCCTGGTGGG
CTGGTGGATCACCCACGAAAAGCTCCCGACCGCCTGGGTGCATGCTTCCTTTATTCAGACCTGGCGGGTGGCCCACGATT
TTCTGGCCTACGATCTTTATATCGAGCAGTTCTACCGCCGGACCATTGTCGTCTGGGTGGCCGGGGCTTCGAGTTTGATG
TCCAAGTTCGATCGCCACCTGGTCGACGGCGTGGTCAACATGATCGGGGTGGCTACGATTGCCGGGGGCGAAGGACTCAA
ACGCAGTGCGCCGGGACAGGCCCAGGCCTATTTGCTCACGATCCTGCTCGGGGTGATCGTGGTGTGCGCTTATCAACTGA
CGACTTTGTTGCGCTAG

Upstream 100 bases:

>100_bases
GTATAATGCTTAAGAAATCGTAAAGTAGCTGGGTAGGCTTGTCTCTGTTCCACGGGTGCAGGACCGGTTTTTGCTCAGCG
CCCCTGGAGAAGATCTGTCG

Downstream 100 bases:

>100_bases
GAGCGGGTGGCGGCGATGCTCAGTTTGCTGGTCTGGTTGCCTATCCTGGGAGCGGCGGTGGTCGGTTGCTGGCCCGCCGG
CGTATCGGCTGTCTGGCCGC

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MTESLLFTSWWIPFYGLIGAVLTLPWATVGVRSGGPRPAAYFNILMAVLALVHGLTLLPAVWGRLPLQLEYTWLETQGMR
LALELEVSPVTIGAVVVITFLSLCAQIYALGYMEKDWALARFFALLGFFEGAICGLAMSNNLLLSYALLEMLTLSTYLLV
GFWYAQPLVVTAARDAFLTKRVGDLLLLMGVVVLANLAPSLNFQDLALWAETADLPPLSATLLGLALIAGPIGKCAQIPL
HFWLDEAMEGPNPASIMRNSLVVACGAYVLIKMQPVLALSPWVLGALLVVGTVTAVGASCVALAQIDIKRALSHSTSAYL
GLVFVAVGLEQNEVALLLLLTHAVAKALLFMSVGAVILITSTQDVTEMGGLWSRLPATTTAFVVGGAASVSLLPLGTFWA
SIYASERLWTWAPWAVGVLLLVNGLTALNLTRVYCRVFLGPRQAKTRRAPEIAWPMSVPMLSLSIINILSPVILLQLGLL
TSVEGPVPTWVFGGSLMASSLAGCLVGWWITHEKLPTAWVHASFIQTWRVAHDFLAYDLYIEQFYRRTIVVWVAGASSLM
SKFDRHLVDGVVNMIGVATIAGGEGLKRSAPGQAQAYLLTILLGVIVVCAYQLTTLLR

Sequences:

>Translated_618_residues
MTESLLFTSWWIPFYGLIGAVLTLPWATVGVRSGGPRPAAYFNILMAVLALVHGLTLLPAVWGRLPLQLEYTWLETQGMR
LALELEVSPVTIGAVVVITFLSLCAQIYALGYMEKDWALARFFALLGFFEGAICGLAMSNNLLLSYALLEMLTLSTYLLV
GFWYAQPLVVTAARDAFLTKRVGDLLLLMGVVVLANLAPSLNFQDLALWAETADLPPLSATLLGLALIAGPIGKCAQIPL
HFWLDEAMEGPNPASIMRNSLVVACGAYVLIKMQPVLALSPWVLGALLVVGTVTAVGASCVALAQIDIKRALSHSTSAYL
GLVFVAVGLEQNEVALLLLLTHAVAKALLFMSVGAVILITSTQDVTEMGGLWSRLPATTTAFVVGGAASVSLLPLGTFWA
SIYASERLWTWAPWAVGVLLLVNGLTALNLTRVYCRVFLGPRQAKTRRAPEIAWPMSVPMLSLSIINILSPVILLQLGLL
TSVEGPVPTWVFGGSLMASSLAGCLVGWWITHEKLPTAWVHASFIQTWRVAHDFLAYDLYIEQFYRRTIVVWVAGASSLM
SKFDRHLVDGVVNMIGVATIAGGEGLKRSAPGQAQAYLLTILLGVIVVCAYQLTTLLR
>Mature_617_residues
TESLLFTSWWIPFYGLIGAVLTLPWATVGVRSGGPRPAAYFNILMAVLALVHGLTLLPAVWGRLPLQLEYTWLETQGMRL
ALELEVSPVTIGAVVVITFLSLCAQIYALGYMEKDWALARFFALLGFFEGAICGLAMSNNLLLSYALLEMLTLSTYLLVG
FWYAQPLVVTAARDAFLTKRVGDLLLLMGVVVLANLAPSLNFQDLALWAETADLPPLSATLLGLALIAGPIGKCAQIPLH
FWLDEAMEGPNPASIMRNSLVVACGAYVLIKMQPVLALSPWVLGALLVVGTVTAVGASCVALAQIDIKRALSHSTSAYLG
LVFVAVGLEQNEVALLLLLTHAVAKALLFMSVGAVILITSTQDVTEMGGLWSRLPATTTAFVVGGAASVSLLPLGTFWAS
IYASERLWTWAPWAVGVLLLVNGLTALNLTRVYCRVFLGPRQAKTRRAPEIAWPMSVPMLSLSIINILSPVILLQLGLLT
SVEGPVPTWVFGGSLMASSLAGCLVGWWITHEKLPTAWVHASFIQTWRVAHDFLAYDLYIEQFYRRTIVVWVAGASSLMS
KFDRHLVDGVVNMIGVATIAGGEGLKRSAPGQAQAYLLTILLGVIVVCAYQLTTLLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=376, Percent_Identity=32.4468085106383, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1788614, Length=409, Percent_Identity=33.2518337408313, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788827, Length=399, Percent_Identity=28.0701754385965, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788831, Length=323, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788829, Length=476, Percent_Identity=26.4705882352941, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788613, Length=339, Percent_Identity=23.598820058997, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI2367154, Length=213, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 66635; Mature: 66504

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESLLFTSWWIPFYGLIGAVLTLPWATVGVRSGGPRPAAYFNILMAVLALVHGLTLLPA
CCCCEEHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VWGRLPLQLEYTWLETQGMRLALELEVSPVTIGAVVVITFLSLCAQIYALGYMEKDWALA
HHCCCCEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RFFALLGFFEGAICGLAMSNNLLLSYALLEMLTLSTYLLVGFWYAQPLVVTAARDAFLTK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHHHHHHHH
RVGDLLLLMGVVVLANLAPSLNFQDLALWAETADLPPLSATLLGLALIAGPIGKCAQIPL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
HFWLDEAMEGPNPASIMRNSLVVACGAYVLIKMQPVLALSPWVLGALLVVGTVTAVGASC
HHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECHHHHHCHHHHHHHHHHHHHHHHHHHH
VALAQIDIKRALSHSTSAYLGLVFVAVGLEQNEVALLLLLTHAVAKALLFMSVGAVILIT
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
STQDVTEMGGLWSRLPATTTAFVVGGAASVSLLPLGTFWASIYASERLWTWAPWAVGVLL
CCCHHHHHHHHHHHCCCCHHHEEECCCCCEEEEEHHHHHHHHHHCCCCEEECHHHHHHHH
LVNGLTALNLTRVYCRVFLGPRQAKTRRAPEIAWPMSVPMLSLSIINILSPVILLQLGLL
HHHCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSVEGPVPTWVFGGSLMASSLAGCLVGWWITHEKLPTAWVHASFIQTWRVAHDFLAYDLY
HCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IEQFYRRTIVVWVAGASSLMSKFDRHLVDGVVNMIGVATIAGGEGLKRSAPGQAQAYLLT
HHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
ILLGVIVVCAYQLTTLLR
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TESLLFTSWWIPFYGLIGAVLTLPWATVGVRSGGPRPAAYFNILMAVLALVHGLTLLPA
CCCEEHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VWGRLPLQLEYTWLETQGMRLALELEVSPVTIGAVVVITFLSLCAQIYALGYMEKDWALA
HHCCCCEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RFFALLGFFEGAICGLAMSNNLLLSYALLEMLTLSTYLLVGFWYAQPLVVTAARDAFLTK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHHHHHHHH
RVGDLLLLMGVVVLANLAPSLNFQDLALWAETADLPPLSATLLGLALIAGPIGKCAQIPL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
HFWLDEAMEGPNPASIMRNSLVVACGAYVLIKMQPVLALSPWVLGALLVVGTVTAVGASC
HHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECHHHHHCHHHHHHHHHHHHHHHHHHHH
VALAQIDIKRALSHSTSAYLGLVFVAVGLEQNEVALLLLLTHAVAKALLFMSVGAVILIT
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
STQDVTEMGGLWSRLPATTTAFVVGGAASVSLLPLGTFWASIYASERLWTWAPWAVGVLL
CCCHHHHHHHHHHHCCCCHHHEEECCCCCEEEEEHHHHHHHHHHCCCCEEECHHHHHHHH
LVNGLTALNLTRVYCRVFLGPRQAKTRRAPEIAWPMSVPMLSLSIINILSPVILLQLGLL
HHHCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TSVEGPVPTWVFGGSLMASSLAGCLVGWWITHEKLPTAWVHASFIQTWRVAHDFLAYDLY
HCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IEQFYRRTIVVWVAGASSLMSKFDRHLVDGVVNMIGVATIAGGEGLKRSAPGQAQAYLLT
HHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
ILLGVIVVCAYQLTTLLR
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]