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The map label for this gene is ndhD [H]

Identifier: 37521651

GI number: 37521651

Start: 2233885

End: 2235417

Strand: Reverse

Name: ndhD [H]

Synonym: gvip277

Alternate gene names: 37521651

Gene position: 2235417-2233885 (Counterclockwise)

Preceding gene: 37521652

Following gene: 37521650

Centisome position: 47.98

GC content: 63.21

Gene sequence:

>1533_bases
ATGCTCAGTTTGCTGGTCTGGTTGCCTATCCTGGGAGCGGCGGTGGTCGGTTGCTGGCCCGCCGGCGTATCGGCTGTCTG
GCCGCGCCGGATTGCTCTGTTGGTGGCGTCTTTAGGGTTGGTGCTCTCGGCGGTCCTTGCCTTCCAGTACAATCCGGCCG
ACGGCGGGCTGCAATTTCGCGAATTTGTCCCCTGGATGACCTCGCTGGGTCTGTCCTACGAACTGGGCATCGACGGGTTG
TCGCTGCCGCTGTTGCTGCTGAATGCCTTTTTGGTCTGGATAGCGATCTTCAGCAGCGACGAGAACGTCCAGCGCCCGCG
CCTCTACTATGCGCTTTTGCTGCTTTTGTGCGGCGGGGTGGTGGGGGCTTTCGCCACCCAGAACGCACTGTTGTTTTTCT
TGTTCTACGAACTGGAACTGATTCCGCTCTATTTGCTGATAGCCATCTGGGGTGGACCCCGGCGCAACTACGCCGCCACC
AAGTTTTTGCTCTATACCGCCATCTCGGGGATCATGGTTTTGGTGGCGTTCTTCGGCCTGGCGGGTCTGGCGGGCATCGC
CAATTATTCCTACGAAGTGCTGGCTGCCCACAATCTGCCCCTGGCCACCCAGTTTCTGCTGTTGGGGGCGGTGCTCGTCG
CTTTTGGCATCAAGATTCCGCTCGTGCCTTTTCACACCTGGCTGCCGGACGCCCACGTCGAGGCGAGCACCCCGGTGTCG
CTGCTGCTGGCGGGGGTGCTCCTCAAGCTCGGAACCTACGGCTTGTTGCGTTTTGGCCTGGGCTTTTTCCCCGAGGCCTG
GAGTGTCGCAGCGCCCTGGCTTGCCGGTTGGGCGGTGGTGAGCGTCCTTTACGGCTGTCTGACGGCCATCGCCCAGAAGG
ACATGAAGAAAATGGTGGCCTACAGTTCGATCGGGCACATGGGCTATATTCTGCTCGCCGCCGCCGCCGCAACGCCTTTG
AGTTTGCTGGGGGCGGTCGCCCAGATGGTGAGCCACGGCCTCATCTCGGGGCTGTTGTTTTTGCTGGTGGGGGTGGTCTA
CAAAAAAACCGGCACCCGCGACATCGACGTGTTGTGCGGTCTGCTGGCCCCGGAGCGCGGATTGCCCTTTGTCGGCAGCC
TGATGATCTTGGCCGTGATGGCCTCCGCGGGCATCCCGAGCATGGTGGGCTTCGTGGCCGAATTCATCATCTTCCGGGCG
AGCTACGCGGTTTTCCCGGTGCAGACCTTGCTGTGCATCCTCGGGACCGGCCTCACGGCGGTCTACCTGCTGATCTTGAT
CAACCGCGCCTTTTTTGGCCGTCTGCCGGCCCAGTTTGCCAACCTGCCGCAGGTGCGCTGGTCGGAGCGACTGCCGGGTC
TGGCCATCGCCCTGCTGGTCGTCTTTTTGGGCCTGCAACCGCAGTGGCTGGTCCGCTGGAGTGAAGCGACCACCGCCGCC
CTCGTCGCCGAGCGGCCCGCCCTGGCGGACGCGCTGCCCGCTAAAGCCGAGCGCACCCACACCGTCGCGGTGCGCCGCGC
CCCTACCCCCTAA

Upstream 100 bases:

>100_bases
GGGACAGGCCCAGGCCTATTTGCTCACGATCCTGCTCGGGGTGATCGTGGTGTGCGCTTATCAACTGACGACTTTGTTGC
GCTAGGAGCGGGTGGCGGCG

Downstream 100 bases:

>100_bases
GGAGAACCGTCCGTGGTGATGTACGACCAGAAAGGCTCTTCCCATCCCCTCGGCAAGGTGATCTTGCGGATGGAGGCGGG
CGGGGCGCTCCTGCCCGACG

Product: NAD(P)H-quinone oxidoreductase subunit D4

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 2; NDH-1, chain 4 2 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLSLLVWLPILGAAVVGCWPAGVSAVWPRRIALLVASLGLVLSAVLAFQYNPADGGLQFREFVPWMTSLGLSYELGIDGL
SLPLLLLNAFLVWIAIFSSDENVQRPRLYYALLLLLCGGVVGAFATQNALLFFLFYELELIPLYLLIAIWGGPRRNYAAT
KFLLYTAISGIMVLVAFFGLAGLAGIANYSYEVLAAHNLPLATQFLLLGAVLVAFGIKIPLVPFHTWLPDAHVEASTPVS
LLLAGVLLKLGTYGLLRFGLGFFPEAWSVAAPWLAGWAVVSVLYGCLTAIAQKDMKKMVAYSSIGHMGYILLAAAAATPL
SLLGAVAQMVSHGLISGLLFLLVGVVYKKTGTRDIDVLCGLLAPERGLPFVGSLMILAVMASAGIPSMVGFVAEFIIFRA
SYAVFPVQTLLCILGTGLTAVYLLILINRAFFGRLPAQFANLPQVRWSERLPGLAIALLVVFLGLQPQWLVRWSEATTAA
LVAERPALADALPAKAERTHTVAVRRAPTP

Sequences:

>Translated_510_residues
MLSLLVWLPILGAAVVGCWPAGVSAVWPRRIALLVASLGLVLSAVLAFQYNPADGGLQFREFVPWMTSLGLSYELGIDGL
SLPLLLLNAFLVWIAIFSSDENVQRPRLYYALLLLLCGGVVGAFATQNALLFFLFYELELIPLYLLIAIWGGPRRNYAAT
KFLLYTAISGIMVLVAFFGLAGLAGIANYSYEVLAAHNLPLATQFLLLGAVLVAFGIKIPLVPFHTWLPDAHVEASTPVS
LLLAGVLLKLGTYGLLRFGLGFFPEAWSVAAPWLAGWAVVSVLYGCLTAIAQKDMKKMVAYSSIGHMGYILLAAAAATPL
SLLGAVAQMVSHGLISGLLFLLVGVVYKKTGTRDIDVLCGLLAPERGLPFVGSLMILAVMASAGIPSMVGFVAEFIIFRA
SYAVFPVQTLLCILGTGLTAVYLLILINRAFFGRLPAQFANLPQVRWSERLPGLAIALLVVFLGLQPQWLVRWSEATTAA
LVAERPALADALPAKAERTHTVAVRRAPTP
>Mature_510_residues
MLSLLVWLPILGAAVVGCWPAGVSAVWPRRIALLVASLGLVLSAVLAFQYNPADGGLQFREFVPWMTSLGLSYELGIDGL
SLPLLLLNAFLVWIAIFSSDENVQRPRLYYALLLLLCGGVVGAFATQNALLFFLFYELELIPLYLLIAIWGGPRRNYAAT
KFLLYTAISGIMVLVAFFGLAGLAGIANYSYEVLAAHNLPLATQFLLLGAVLVAFGIKIPLVPFHTWLPDAHVEASTPVS
LLLAGVLLKLGTYGLLRFGLGFFPEAWSVAAPWLAGWAVVSVLYGCLTAIAQKDMKKMVAYSSIGHMGYILLAAAAATPL
SLLGAVAQMVSHGLISGLLFLLVGVVYKKTGTRDIDVLCGLLAPERGLPFVGSLMILAVMASAGIPSMVGFVAEFIIFRA
SYAVFPVQTLLCILGTGLTAVYLLILINRAFFGRLPAQFANLPQVRWSERLPGLAIALLVVFLGLQPQWLVRWSEATTAA
LVAERPALADALPAKAERTHTVAVRRAPTP

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=348, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788613, Length=476, Percent_Identity=37.3949579831933, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788831, Length=195, Percent_Identity=35.8974358974359, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788827, Length=299, Percent_Identity=26.4214046822742, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788829, Length=347, Percent_Identity=29.3948126801153, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693160, Length=392, Percent_Identity=28.8265306122449, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367154, Length=219, Percent_Identity=29.6803652968037, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788614, Length=499, Percent_Identity=25.6513026052104, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54721; Mature: 54721

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLLVWLPILGAAVVGCWPAGVSAVWPRRIALLVASLGLVLSAVLAFQYNPADGGLQFR
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EFVPWMTSLGLSYELGIDGLSLPLLLLNAFLVWIAIFSSDENVQRPRLYYALLLLLCGGV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VGAFATQNALLFFLFYELELIPLYLLIAIWGGPRRNYAATKFLLYTAISGIMVLVAFFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGLAGIANYSYEVLAAHNLPLATQFLLLGAVLVAFGIKIPLVPFHTWLPDAHVEASTPVS
HHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCCCCCHH
LLLAGVLLKLGTYGLLRFGLGFFPEAWSVAAPWLAGWAVVSVLYGCLTAIAQKDMKKMVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSIGHMGYILLAAAAATPLSLLGAVAQMVSHGLISGLLFLLVGVVYKKTGTRDIDVLCG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLAPERGLPFVGSLMILAVMASAGIPSMVGFVAEFIIFRASYAVFPVQTLLCILGTGLTA
HHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VYLLILINRAFFGRLPAQFANLPQVRWSERLPGLAIALLVVFLGLQPQWLVRWSEATTAA
HHHHHHHHHHHHHCCCHHHCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHEEEECHHHHHH
LVAERPALADALPAKAERTHTVAVRRAPTP
HHHCCCHHHHHCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MLSLLVWLPILGAAVVGCWPAGVSAVWPRRIALLVASLGLVLSAVLAFQYNPADGGLQFR
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
EFVPWMTSLGLSYELGIDGLSLPLLLLNAFLVWIAIFSSDENVQRPRLYYALLLLLCGGV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VGAFATQNALLFFLFYELELIPLYLLIAIWGGPRRNYAATKFLLYTAISGIMVLVAFFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGLAGIANYSYEVLAAHNLPLATQFLLLGAVLVAFGIKIPLVPFHTWLPDAHVEASTPVS
HHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCCCCCHH
LLLAGVLLKLGTYGLLRFGLGFFPEAWSVAAPWLAGWAVVSVLYGCLTAIAQKDMKKMVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSIGHMGYILLAAAAATPLSLLGAVAQMVSHGLISGLLFLLVGVVYKKTGTRDIDVLCG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLAPERGLPFVGSLMILAVMASAGIPSMVGFVAEFIIFRASYAVFPVQTLLCILGTGLTA
HHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VYLLILINRAFFGRLPAQFANLPQVRWSERLPGLAIALLVVFLGLQPQWLVRWSEATTAA
HHHHHHHHHHHHHCCCHHHCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHEEEECHHHHHH
LVAERPALADALPAKAERTHTVAVRRAPTP
HHHCCCHHHHHCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]