Click here to switch to the map view.

The map label for this gene is dfrA [H]

Identifier: 37521316

GI number: 37521316

Start: 1852612

End: 1853625

Strand: Direct

Name: dfrA [H]

Synonym: glr1747

Alternate gene names: 37521316

Gene position: 1852612-1853625 (Clockwise)

Preceding gene: 37521315

Following gene: 37521317

Centisome position: 39.76

GC content: 66.57

Gene sequence:

>1014_bases
ATGACCCTGCGCGTGTTCGTGACGGGCGGCACCGGCTTCGTGGGAGCCAACCTCGTGCGCTTGCTGCTCACCGAAGGTTA
CAGCGTGCGCGCCCTGGTGCGTGAACCTGCGAAGGCACAAGATCTGGCGGCCCTCGGTGCCGAAGTGGTAGCCGGAGATC
TGGGCAGTCGCGCTCTGGCTGGGCAGATGGCGGGCTGCCGGGCGCTCTTTCACGTCGCCGCCCACTACAGCTTGCTGCAG
GCCGATCGCGAGCGGCTCTGGCAATCGAACGTGCTCGGAACCCGCAACGTGCTCGCGGCAGCCCGCGAGGCAGGGGTAGA
GCGGACGGTCTACACCTCATCGGTGGCGGCCATCGGCGTCAAAAGCCCCCGCGCCGATGAAAACCACCAGAGCCCACCGG
ACAAACTCGTGGGCGTCTACAAACAGTCGAAATATTGGGCGGAGCAGGAAGCACGTCGGGCCGCTGCCCGCGGCCAGGAT
GTGGTGATCGTCAACCCGACCGCGCCAGTGGGTCCCTTCGACAGCAAACCGACGCCGACCGGCGACATCGTGTTGCGCTT
CTTGCGCCGCCAGATGCCCTTTTATCTGGAGACCGGCCTCAACTTCGTCCACGTGCGCGACGTGGTCCAGGGGCACCTGG
CTGCCCTGGAGCGGGGCCGCTCAGGGGAGCGCTACATCCTCGGACACCGAGATTTGTCTTTCAAAGCGCTGCTCGACACG
CTGGCGGAGGTGACCGGCATACCAGCCCCACAGATGGCTGTTCCCGATTGGCTGCCCCTGGGAGTGGCCTGGATCGACGA
GCAGCTGCTCTCGCCCTTGCTCGGCCGCCCCCCCAGCGTGCCCATCGACGGCGTGCGCATGGCCGCCCAGCCGATGTACT
ACGACGCCTCGAAGGCCGTGCGCGAACTGGGGCTGCCCCAGACCCCCGTCCGGGAAGCGCTCGTCGATGCCGTAGACTGG
TTTAGAAAGAACGGATACATCGGCAACGGGGGGGGATTCCGGCATGGCGGTTAG

Upstream 100 bases:

>100_bases
CCATCATCGGCGGGCTGTTGACCTCGACGCTGCTCAGCCTTATCGTGGTCCCCGTTGCCTATAGCCTGTTCGCCCCCTTG
AAAGAATCCAAACACACCCT

Downstream 100 bases:

>100_bases
TCTCAAACAAGCGTTCGAAGTCGGAAAGTATTTGATTTCCCAGCGTCTCGCGGGCCGCGAGCGCTTCCCGCTGGTGTTGA
TGCTCGAGCCGCTGTTTCGC

Product: dihydrokaempferol 4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTLRVFVTGGTGFVGANLVRLLLTEGYSVRALVREPAKAQDLAALGAEVVAGDLGSRALAGQMAGCRALFHVAAHYSLLQ
ADRERLWQSNVLGTRNVLAAAREAGVERTVYTSSVAAIGVKSPRADENHQSPPDKLVGVYKQSKYWAEQEARRAAARGQD
VVIVNPTAPVGPFDSKPTPTGDIVLRFLRRQMPFYLETGLNFVHVRDVVQGHLAALERGRSGERYILGHRDLSFKALLDT
LAEVTGIPAPQMAVPDWLPLGVAWIDEQLLSPLLGRPPSVPIDGVRMAAQPMYYDASKAVRELGLPQTPVREALVDAVDW
FRKNGYIGNGGGFRHGG

Sequences:

>Translated_337_residues
MTLRVFVTGGTGFVGANLVRLLLTEGYSVRALVREPAKAQDLAALGAEVVAGDLGSRALAGQMAGCRALFHVAAHYSLLQ
ADRERLWQSNVLGTRNVLAAAREAGVERTVYTSSVAAIGVKSPRADENHQSPPDKLVGVYKQSKYWAEQEARRAAARGQD
VVIVNPTAPVGPFDSKPTPTGDIVLRFLRRQMPFYLETGLNFVHVRDVVQGHLAALERGRSGERYILGHRDLSFKALLDT
LAEVTGIPAPQMAVPDWLPLGVAWIDEQLLSPLLGRPPSVPIDGVRMAAQPMYYDASKAVRELGLPQTPVREALVDAVDW
FRKNGYIGNGGGFRHGG
>Mature_336_residues
TLRVFVTGGTGFVGANLVRLLLTEGYSVRALVREPAKAQDLAALGAEVVAGDLGSRALAGQMAGCRALFHVAAHYSLLQA
DRERLWQSNVLGTRNVLAAAREAGVERTVYTSSVAAIGVKSPRADENHQSPPDKLVGVYKQSKYWAEQEARRAAARGQDV
VIVNPTAPVGPFDSKPTPTGDIVLRFLRRQMPFYLETGLNFVHVRDVVQGHLAALERGRSGERYILGHRDLSFKALLDTL
AEVTGIPAPQMAVPDWLPLGVAWIDEQLLSPLLGRPPSVPIDGVRMAAQPMYYDASKAVRELGLPQTPVREALVDAVDWF
RKNGYIGNGGGFRHGG

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=340, Percent_Identity=25.2941176470588, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI8393516, Length=340, Percent_Identity=25.2941176470588, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI116268111, Length=368, Percent_Identity=27.9891304347826, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310132178, Length=344, Percent_Identity=27.3255813953488, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI310113012, Length=344, Percent_Identity=27.3255813953488, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=337, Percent_Identity=30.5637982195846, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36492; Mature: 36361

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRVFVTGGTGFVGANLVRLLLTEGYSVRALVREPAKAQDLAALGAEVVAGDLGSRALA
CEEEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
GQMAGCRALFHVAAHYSLLQADRERLWQSNVLGTRNVLAAAREAGVERTVYTSSVAAIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHEEEC
KSPRADENHQSPPDKLVGVYKQSKYWAEQEARRAAARGQDVVIVNPTAPVGPFDSKPTPT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCH
GDIVLRFLRRQMPFYLETGLNFVHVRDVVQGHLAALERGRSGERYILGHRDLSFKALLDT
HHHHHHHHHHHCCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
LAEVTGIPAPQMAVPDWLPLGVAWIDEQLLSPLLGRPPSVPIDGVRMAAQPMYYDASKAV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHH
RELGLPQTPVREALVDAVDWFRKNGYIGNGGGFRHGG
HHCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCC
>Mature Secondary Structure 
TLRVFVTGGTGFVGANLVRLLLTEGYSVRALVREPAKAQDLAALGAEVVAGDLGSRALA
EEEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
GQMAGCRALFHVAAHYSLLQADRERLWQSNVLGTRNVLAAAREAGVERTVYTSSVAAIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHEEEC
KSPRADENHQSPPDKLVGVYKQSKYWAEQEARRAAARGQDVVIVNPTAPVGPFDSKPTPT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCH
GDIVLRFLRRQMPFYLETGLNFVHVRDVVQGHLAALERGRSGERYILGHRDLSFKALLDT
HHHHHHHHHHHCCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
LAEVTGIPAPQMAVPDWLPLGVAWIDEQLLSPLLGRPPSVPIDGVRMAAQPMYYDASKAV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHH
RELGLPQTPVREALVDAVDWFRKNGYIGNGGGFRHGG
HHCCCCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]