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The map label for this gene is aspA [H]

Identifier: 37521026

GI number: 37521026

Start: 1563061

End: 1564470

Strand: Direct

Name: aspA [H]

Synonym: glr1457

Alternate gene names: 37521026

Gene position: 1563061-1564470 (Clockwise)

Preceding gene: 37521018

Following gene: 37521028

Centisome position: 33.55

GC content: 65.6

Gene sequence:

>1410_bases
ATGCAACCAAGAACCGAGCGCGACTCGATGGGCGAGCGCACCCTGGAGGCCGGAGCCTATTACGGCATCCAGACCCTGCG
CGCCGTCGAGAATTTCCCGATTAGCGGCATCGGCCCGCTGCCCGACTTCGTGCGCGCCTGCACCCTCATCAAACGGGCCG
CCGCCCGGGTGAACGCCGAGCTTGAATGCATCCCCAAAGACGTGGCCCAGGCGATCATTGGCGCCTCGGACGAGGTGCTG
GCGGGCCAGTGGAGCGATCAGTTCGTGGTCGATATCTTTCAGGCGGGCGCCGGCACCTCCCACCACATGAATATCAACGA
GGTGCTCGCCAACCGCGCCCTCGAGCTGCTGGGTTACGCGCGGGGCGACTACAAGCAGGTCAACCCCAACGATCACGTCA
ACTACGGCCAGTCCACCAACGACGTGATCCCCACTGCCATCCGTCTGGGAGCGCTCTTTGCAAGCGCGCCGCTGTTGGAG
GCGCTAAGCACCCTGCAACAAAGTTTCGAGGCCAAGGGCAACGAGTTTTTGCCCATCGTCAAATCCGGGCGCACCCACCT
GCAGGACGCGGTGCCCATAAGGCTTGGGGATGAATTTCTGGCCTATGCCCAGATCGTCGGCGAGCACCGCGCCCGCATCG
AAACCGCCTGCCGCGAGCTATTGGTGCTGGGCCTGGGGGGCAGCGCCGCCGGTACCGGCCTCAACACCCATCCCCAGTAC
CGGGTGCGCGTCGTCGCCGAACTGGCCCGGCTGACAGGGCACCCCCTCACCCCCGCCCCCCGACCGATGGCGGCAATGCA
GAGCATGGCGCCCTTCGTGGCCGTCTCCGGCAGCCTGCGCAATCTGGCCCAGGATCTGGTCAAGATCGCGGGCGATCTGC
GCCTGATGGATTCCGGCCCCAAGACGGGCCTGCGTGAGATCGAACTGCCGCCGGTGCAGCCGGGTTCGTCGATCATGCCC
GGCAAGTACAACCCGGTGCTGTGCGAGATGCTCACGATGGTCGCCTTTCAGGTGATGGGCTACGACCAGGCGATTGCCTT
GGCCGCCCAGGCGGGCCAACTGGAATTGAACGTGATGATGCCGCTGATCGCCTACGACTTGCTCCACAGCTTCACGATCC
TCACCAACGCCCTCACGGTCTTTCGAGCGCGCTGCATCGACGGCATCCAGGCCCAACCCGAGCGCTGCCTGGCCTACGCC
GAGGGCTCGATCGCCCTGGTGACCGCTCTCAATCCCCACATCGGCTATCTCAACGCCGCCGCCGTCGCCAAAGAATCGCT
CGCGACCGGCCGCTCCCTGCGCGAGATCGTTCTCGAGCGGGGGCTACTGACCGAAGCGCAACTCGCCGAGATCCTCGACC
TTGAAGCGATGAGCCGGTTGCCGGAGACGCAGGCCGCCTCAGACCGGTAG

Upstream 100 bases:

>100_bases
CATCTCTCATTAGTAACTTTTTTATGCCCATCCGCAAAGACGGGTCGGACAGGTTCCGATTCGTGGCACGATCGGCAAGT
CTTCCTGCTCACTGACCGCC

Downstream 100 bases:

>100_bases
GCGGATGCGCTGCAATGCCTCGGAAGCCGCAACCGCCTTGTTGATTTCGAGGTTCAGCATGACGACGTGGACGACAGCCC
GGGCACAAAGTTCTCCTTTG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQPRTERDSMGERTLEAGAYYGIQTLRAVENFPISGIGPLPDFVRACTLIKRAAARVNAELECIPKDVAQAIIGASDEVL
AGQWSDQFVVDIFQAGAGTSHHMNINEVLANRALELLGYARGDYKQVNPNDHVNYGQSTNDVIPTAIRLGALFASAPLLE
ALSTLQQSFEAKGNEFLPIVKSGRTHLQDAVPIRLGDEFLAYAQIVGEHRARIETACRELLVLGLGGSAAGTGLNTHPQY
RVRVVAELARLTGHPLTPAPRPMAAMQSMAPFVAVSGSLRNLAQDLVKIAGDLRLMDSGPKTGLREIELPPVQPGSSIMP
GKYNPVLCEMLTMVAFQVMGYDQAIALAAQAGQLELNVMMPLIAYDLLHSFTILTNALTVFRARCIDGIQAQPERCLAYA
EGSIALVTALNPHIGYLNAAAVAKESLATGRSLREIVLERGLLTEAQLAEILDLEAMSRLPETQAASDR

Sequences:

>Translated_469_residues
MQPRTERDSMGERTLEAGAYYGIQTLRAVENFPISGIGPLPDFVRACTLIKRAAARVNAELECIPKDVAQAIIGASDEVL
AGQWSDQFVVDIFQAGAGTSHHMNINEVLANRALELLGYARGDYKQVNPNDHVNYGQSTNDVIPTAIRLGALFASAPLLE
ALSTLQQSFEAKGNEFLPIVKSGRTHLQDAVPIRLGDEFLAYAQIVGEHRARIETACRELLVLGLGGSAAGTGLNTHPQY
RVRVVAELARLTGHPLTPAPRPMAAMQSMAPFVAVSGSLRNLAQDLVKIAGDLRLMDSGPKTGLREIELPPVQPGSSIMP
GKYNPVLCEMLTMVAFQVMGYDQAIALAAQAGQLELNVMMPLIAYDLLHSFTILTNALTVFRARCIDGIQAQPERCLAYA
EGSIALVTALNPHIGYLNAAAVAKESLATGRSLREIVLERGLLTEAQLAEILDLEAMSRLPETQAASDR
>Mature_469_residues
MQPRTERDSMGERTLEAGAYYGIQTLRAVENFPISGIGPLPDFVRACTLIKRAAARVNAELECIPKDVAQAIIGASDEVL
AGQWSDQFVVDIFQAGAGTSHHMNINEVLANRALELLGYARGDYKQVNPNDHVNYGQSTNDVIPTAIRLGALFASAPLLE
ALSTLQQSFEAKGNEFLPIVKSGRTHLQDAVPIRLGDEFLAYAQIVGEHRARIETACRELLVLGLGGSAAGTGLNTHPQY
RVRVVAELARLTGHPLTPAPRPMAAMQSMAPFVAVSGSLRNLAQDLVKIAGDLRLMDSGPKTGLREIELPPVQPGSSIMP
GKYNPVLCEMLTMVAFQVMGYDQAIALAAQAGQLELNVMMPLIAYDLLHSFTILTNALTVFRARCIDGIQAQPERCLAYA
EGSIALVTALNPHIGYLNAAAVAKESLATGRSLREIVLERGLLTEAQLAEILDLEAMSRLPETQAASDR

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=458, Percent_Identity=44.1048034934498, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI87082375, Length=457, Percent_Identity=49.2341356673961, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787896, Length=455, Percent_Identity=44.8351648351648, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17553882, Length=458, Percent_Identity=43.4497816593886, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=322, Percent_Identity=42.8571428571429, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324993, Length=458, Percent_Identity=43.8864628820961, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640179, Length=459, Percent_Identity=44.4444444444444, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640177, Length=459, Percent_Identity=44.4444444444444, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78710009, Length=460, Percent_Identity=41.304347826087, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=41.1255411255411, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24583245, Length=448, Percent_Identity=36.8303571428571, Blast_Score=314, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50511; Mature: 50511

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPRTERDSMGERTLEAGAYYGIQTLRAVENFPISGIGPLPDFVRACTLIKRAAARVNAE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LECIPKDVAQAIIGASDEVLAGQWSDQFVVDIFQAGAGTSHHMNINEVLANRALELLGYA
CEECCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
RGDYKQVNPNDHVNYGQSTNDVIPTAIRLGALFASAPLLEALSTLQQSFEAKGNEFLPIV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHH
KSGRTHLQDAVPIRLGDEFLAYAQIVGEHRARIETACRELLVLGLGGSAAGTGLNTHPQY
HCCCHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
RVRVVAELARLTGHPLTPAPRPMAAMQSMAPFVAVSGSLRNLAQDLVKIAGDLRLMDSGP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHCCCEEECCCC
KTGLREIELPPVQPGSSIMPGKYNPVLCEMLTMVAFQVMGYDQAIALAAQAGQLELNVMM
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHH
PLIAYDLLHSFTILTNALTVFRARCIDGIQAQPERCLAYAEGSIALVTALNPHIGYLNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHH
AVAKESLATGRSLREIVLERGLLTEAQLAEILDLEAMSRLPETQAASDR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQPRTERDSMGERTLEAGAYYGIQTLRAVENFPISGIGPLPDFVRACTLIKRAAARVNAE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LECIPKDVAQAIIGASDEVLAGQWSDQFVVDIFQAGAGTSHHMNINEVLANRALELLGYA
CEECCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
RGDYKQVNPNDHVNYGQSTNDVIPTAIRLGALFASAPLLEALSTLQQSFEAKGNEFLPIV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHH
KSGRTHLQDAVPIRLGDEFLAYAQIVGEHRARIETACRELLVLGLGGSAAGTGLNTHPQY
HCCCHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
RVRVVAELARLTGHPLTPAPRPMAAMQSMAPFVAVSGSLRNLAQDLVKIAGDLRLMDSGP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHCCCEEECCCC
KTGLREIELPPVQPGSSIMPGKYNPVLCEMLTMVAFQVMGYDQAIALAAQAGQLELNVMM
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHH
PLIAYDLLHSFTILTNALTVFRARCIDGIQAQPERCLAYAEGSIALVTALNPHIGYLNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHH
AVAKESLATGRSLREIVLERGLLTEAQLAEILDLEAMSRLPETQAASDR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]