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The map label for this gene is birA [H]

Identifier: 37520907

GI number: 37520907

Start: 1430461

End: 1431237

Strand: Reverse

Name: birA [H]

Synonym: gll1338

Alternate gene names: 37520907

Gene position: 1431237-1430461 (Counterclockwise)

Preceding gene: 37520910

Following gene: 37520906

Centisome position: 30.72

GC content: 64.74

Gene sequence:

>777_bases
ATGATCCTGGATAAGCGCAAATTTCAACTGCACTTAAAGACAGAAACCCTCGGCCACGGGTTTCATCTGTACGAGCGGAT
CGATTCGACCAATCGCCTGGCGATGGAGTTTTTGCGCGAGGGCGCTGCCGAGGGCACGACCATCCTCGCCGAGCAGCAGA
CCGGCGGACGCGGCAGCAACGGTCGCCAGTGGGAATCGCTTCCCGGCGGGCTGTACCTGTCGGTGATCCTCAGGCCGCAA
CTGAAGCTCGCCGATATCTTTCAATTGACGTTGGTGGCAGCCTTTGGCGTCGCCCAGAGCCTCGGCCGCCTTACCGGGGC
CGATGTGCGTCTCAAGTGGCCTAACGACCTGGTTATCGAGCAGGGTAGGCGCCTTGCCAAAGTCGGCGGCATCCTCACCG
AGACCCGCATCCAGGGCGACCGGCTCGCGGGGGCGGTGGTGGGTATCGGCATCAACTGGGACAACCCGGTGCCCGCCGAG
GCGGCCCGCCTCAAGCCGCTCTCGCGGCGGGATCTCGACTTGGCGCCGGTGGCGGCGGCGGTGCTTCTGGGCCTGGAACA
GAGCTATCAGCTCTGGCACCAGCGCGGTATCGAGCGCATCGTGAGCGGCTACGAACGCTATCTGGTGAACCTGGGTCAGG
CGGTGGAGGTGCCCGGCCACGACGGCCAGGGTCGGATCATCGGCATCGACGAGCGTGGGGCGTTGCGGGTATTGTTTTTA
GACGGCGGTGAGGCGCTGGTCATGCCCAGCGAATTGCGGCTGGGCTACGCGCTGTGA

Upstream 100 bases:

>100_bases
CGCCCAGCAAAGCCGCTGCTAAAATGAACGCTATTCCTGTCTTGGCTGCTTCGGTAAACAGCATGGCTACGCCCCAAAAA
TAAGATTTACCCCTATCTCA

Downstream 100 bases:

>100_bases
GGATCAAGCTGGTCGCGGTCGGACGGCTGCGCGAGGAGGCTTACGAGCGGGCCTGTGCAGAGTACGCCCGGCGGCTGGCA
GCCTATGCCCGCCTGGAATT

Product: biotin acetyl-CoA carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MILDKRKFQLHLKTETLGHGFHLYERIDSTNRLAMEFLREGAAEGTTILAEQQTGGRGSNGRQWESLPGGLYLSVILRPQ
LKLADIFQLTLVAAFGVAQSLGRLTGADVRLKWPNDLVIEQGRRLAKVGGILTETRIQGDRLAGAVVGIGINWDNPVPAE
AARLKPLSRRDLDLAPVAAAVLLGLEQSYQLWHQRGIERIVSGYERYLVNLGQAVEVPGHDGQGRIIGIDERGALRVLFL
DGGEALVMPSELRLGYAL

Sequences:

>Translated_258_residues
MILDKRKFQLHLKTETLGHGFHLYERIDSTNRLAMEFLREGAAEGTTILAEQQTGGRGSNGRQWESLPGGLYLSVILRPQ
LKLADIFQLTLVAAFGVAQSLGRLTGADVRLKWPNDLVIEQGRRLAKVGGILTETRIQGDRLAGAVVGIGINWDNPVPAE
AARLKPLSRRDLDLAPVAAAVLLGLEQSYQLWHQRGIERIVSGYERYLVNLGQAVEVPGHDGQGRIIGIDERGALRVLFL
DGGEALVMPSELRLGYAL
>Mature_258_residues
MILDKRKFQLHLKTETLGHGFHLYERIDSTNRLAMEFLREGAAEGTTILAEQQTGGRGSNGRQWESLPGGLYLSVILRPQ
LKLADIFQLTLVAAFGVAQSLGRLTGADVRLKWPNDLVIEQGRRLAKVGGILTETRIQGDRLAGAVVGIGINWDNPVPAE
AARLKPLSRRDLDLAPVAAAVLLGLEQSYQLWHQRGIERIVSGYERYLVNLGQAVEVPGHDGQGRIIGIDERGALRVLFL
DGGEALVMPSELRLGYAL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=262, Percent_Identity=28.6259541984733, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790408, Length=217, Percent_Identity=33.1797235023041, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28287; Mature: 28287

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDKRKFQLHLKTETLGHGFHLYERIDSTNRLAMEFLREGAAEGTTILAEQQTGGRGSN
CCCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
GRQWESLPGGLYLSVILRPQLKLADIFQLTLVAAFGVAQSLGRLTGADVRLKWPNDLVIE
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
QGRRLAKVGGILTETRIQGDRLAGAVVGIGINWDNPVPAEAARLKPLSRRDLDLAPVAAA
CCCHHHHHCCCHHEEECCCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHH
VLLGLEQSYQLWHQRGIERIVSGYERYLVNLGQAVEVPGHDGQGRIIGIDERGALRVLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCEEEEEE
DGGEALVMPSELRLGYAL
ECCCEEEECHHHCCCCCC
>Mature Secondary Structure
MILDKRKFQLHLKTETLGHGFHLYERIDSTNRLAMEFLREGAAEGTTILAEQQTGGRGSN
CCCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
GRQWESLPGGLYLSVILRPQLKLADIFQLTLVAAFGVAQSLGRLTGADVRLKWPNDLVIE
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
QGRRLAKVGGILTETRIQGDRLAGAVVGIGINWDNPVPAEAARLKPLSRRDLDLAPVAAA
CCCHHHHHCCCHHEEECCCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHH
VLLGLEQSYQLWHQRGIERIVSGYERYLVNLGQAVEVPGHDGQGRIIGIDERGALRVLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCEEEEEE
DGGEALVMPSELRLGYAL
ECCCEEEECHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]