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The map label for this gene is rpoD [H]

Identifier: 37520903

GI number: 37520903

Start: 1426808

End: 1427860

Strand: Reverse

Name: rpoD [H]

Synonym: gll1334

Alternate gene names: 37520903

Gene position: 1427860-1426808 (Counterclockwise)

Preceding gene: 37520904

Following gene: 37520902

Centisome position: 30.65

GC content: 62.87

Gene sequence:

>1053_bases
ATGAGACAGCCGATGCCGAGCGACGACCTCTGGTCCGACGAAGTGCTGGTAGAAGCGGAAGCGGAGACCGAGGTCCTCGC
CCCCGAGGAATTTACCGTCGTCCGCGGTAGCACCGACGATCTGGTGCGGCTGTATCTACAAGAAATCGGCCGGGTGGCGC
TATTGAAGCCTGCCGAAGAAATCGAACTGGCCCGCCGCATTGCCACCTGGCTTTCACTCGAAGAAAAGCGCAGCAAATTT
CAGGCCGTCGCTGCCGAATCGTGGCTCGAATCTGCGAATCTGACGCCCCAGCAGTGGTCCACCATCGTCCGCGAGGGCGA
GCGCGCCAAGGCCCATCTGGTCAAGGCCAACCTGCGTCTGGTGGTCTCGGTGGCCAAAAAATACCAGGGGCGCGGCCTGC
ATCTGCTCGATCTCATCCAGGAAGGCACCCTCGGCCTGATCCGCGCCGCCGAAAAGTTCGATTACACCCGCGGTTTCAAG
TTTTCGACCTACGCGACCTGGTGGATCCGCCAGGGTATCACCCGCGCCATCGCCATGCAGGCGCGCACGATCCGCTTGCC
GGTCCACATCGTCGAGAAGGTCAACCGCATCAAAAAGGCTACCCGCCAACTCTCGCAGCAGTTGGGCCGCTCGCCTTCGG
AGGATGAGATTGCCCAGGCGGTGGAGATGGACGCGGATCAACTGCGCTTTGTGCGCAAGGCCATCCAGTTGCCGGTGTCG
CTGGAAACCCCCGTTGGGCGCGAGGAAGATACCGCCCTGGGCGATCTCATCCAGGCGAAGGGCAACGAGCCCGAGCACGG
TGTGGTGCGCGATTTGATGAACCAGGATCTCGAATCGCTGCTCCAGACTCTGACACCCCGCGAGCGCGACGTGCTCCGGT
TGCGCTACGGCCTCATCGACGGCCGCTCGCGCACCCTGGATGAAGTTGGCCAGCAATTCGGGCTCACCCGCGAGCGCATC
CGTCAGATCGAGGCGCGGGCGCTGCGCAAACTGCGCCACCCGCAAAGGCTCCAGCGCGTGCGCGAGTATCTCGAAGGCAT
CGAGAATACGTAA

Upstream 100 bases:

>100_bases
CGGTGGAGTCATCAGCTAAAGAAAACGTTAAACAAACCTGGCGCGGATCCTCTCTACCCCATACTGGGGTGTAATCGCAC
TTCTTGCAGTGTGAAAGGGG

Downstream 100 bases:

>100_bases
TTTACTTCTCCAAATGTGTGCGTTTTTACTGTGCTCAGCAATTGCTACGTCAGTTAAGTTGATAAGCCCAATCTCTCATC
GCTCGGCGCACCCAGACTTC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRQPMPSDDLWSDEVLVEAEAETEVLAPEEFTVVRGSTDDLVRLYLQEIGRVALLKPAEEIELARRIATWLSLEEKRSKF
QAVAAESWLESANLTPQQWSTIVREGERAKAHLVKANLRLVVSVAKKYQGRGLHLLDLIQEGTLGLIRAAEKFDYTRGFK
FSTYATWWIRQGITRAIAMQARTIRLPVHIVEKVNRIKKATRQLSQQLGRSPSEDEIAQAVEMDADQLRFVRKAIQLPVS
LETPVGREEDTALGDLIQAKGNEPEHGVVRDLMNQDLESLLQTLTPRERDVLRLRYGLIDGRSRTLDEVGQQFGLTRERI
RQIEARALRKLRHPQRLQRVREYLEGIENT

Sequences:

>Translated_350_residues
MRQPMPSDDLWSDEVLVEAEAETEVLAPEEFTVVRGSTDDLVRLYLQEIGRVALLKPAEEIELARRIATWLSLEEKRSKF
QAVAAESWLESANLTPQQWSTIVREGERAKAHLVKANLRLVVSVAKKYQGRGLHLLDLIQEGTLGLIRAAEKFDYTRGFK
FSTYATWWIRQGITRAIAMQARTIRLPVHIVEKVNRIKKATRQLSQQLGRSPSEDEIAQAVEMDADQLRFVRKAIQLPVS
LETPVGREEDTALGDLIQAKGNEPEHGVVRDLMNQDLESLLQTLTPRERDVLRLRYGLIDGRSRTLDEVGQQFGLTRERI
RQIEARALRKLRHPQRLQRVREYLEGIENT
>Mature_350_residues
MRQPMPSDDLWSDEVLVEAEAETEVLAPEEFTVVRGSTDDLVRLYLQEIGRVALLKPAEEIELARRIATWLSLEEKRSKF
QAVAAESWLESANLTPQQWSTIVREGERAKAHLVKANLRLVVSVAKKYQGRGLHLLDLIQEGTLGLIRAAEKFDYTRGFK
FSTYATWWIRQGITRAIAMQARTIRLPVHIVEKVNRIKKATRQLSQQLGRSPSEDEIAQAVEMDADQLRFVRKAIQLPVS
LETPVGREEDTALGDLIQAKGNEPEHGVVRDLMNQDLESLLQTLTPRERDVLRLRYGLIDGRSRTLDEVGQQFGLTRERI
RQIEARALRKLRHPQRLQRVREYLEGIENT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=50, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1789098, Length=328, Percent_Identity=39.9390243902439, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=27.5, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 40142; Mature: 40142

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQPMPSDDLWSDEVLVEAEAETEVLAPEEFTVVRGSTDDLVRLYLQEIGRVALLKPAEE
CCCCCCCCCCCCCCEEEEECCCCCEECCCCEEEEECCHHHHHHHHHHHHCCEECCCCHHH
IELARRIATWLSLEEKRSKFQAVAAESWLESANLTPQQWSTIVREGERAKAHLVKANLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
VVSVAKKYQGRGLHLLDLIQEGTLGLIRAAEKFDYTRGFKFSTYATWWIRQGITRAIAMQ
HHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTIRLPVHIVEKVNRIKKATRQLSQQLGRSPSEDEIAQAVEMDADQLRFVRKAIQLPVS
HHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
LETPVGREEDTALGDLIQAKGNEPEHGVVRDLMNQDLESLLQTLTPRERDVLRLRYGLID
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GRSRTLDEVGQQFGLTRERIRQIEARALRKLRHPQRLQRVREYLEGIENT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRQPMPSDDLWSDEVLVEAEAETEVLAPEEFTVVRGSTDDLVRLYLQEIGRVALLKPAEE
CCCCCCCCCCCCCCEEEEECCCCCEECCCCEEEEECCHHHHHHHHHHHHCCEECCCCHHH
IELARRIATWLSLEEKRSKFQAVAAESWLESANLTPQQWSTIVREGERAKAHLVKANLRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
VVSVAKKYQGRGLHLLDLIQEGTLGLIRAAEKFDYTRGFKFSTYATWWIRQGITRAIAMQ
HHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTIRLPVHIVEKVNRIKKATRQLSQQLGRSPSEDEIAQAVEMDADQLRFVRKAIQLPVS
HHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
LETPVGREEDTALGDLIQAKGNEPEHGVVRDLMNQDLESLLQTLTPRERDVLRLRYGLID
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GRSRTLDEVGQQFGLTRERIRQIEARALRKLRHPQRLQRVREYLEGIENT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]