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The map label for this gene is phrA [H]

Identifier: 37520353

GI number: 37520353

Start: 835959

End: 837398

Strand: Direct

Name: phrA [H]

Synonym: gvip090

Alternate gene names: 37520353

Gene position: 835959-837398 (Clockwise)

Preceding gene: 37520352

Following gene: 37520354

Centisome position: 17.94

GC content: 64.58

Gene sequence:

>1440_bases
ATGTCTTCTATTGCGATTGTCTGGCACCGTCGGGATCTCAGAGTGCACGACAACCCTGCTCTCTGGCAGGCAAGCCGCAC
GGGCGGGCAGGTGCTCGCCGTTTTCATCGTCGATCCGACCATTGTCGAGCGCGATGACACCGCCCCGGCACGGATCTATT
TTCTGCGCGAATCGGTGCTGGAATTGCAAAAGGCCTACCGCACCATCGGCGGCCGTCTGGCGGTGCGCGTGGGCGAGCCG
GTGCAGCAACTGGTGGCCCTGGCCCAAGCCGTGGGAGCCGGGGCGGTCTATTTTAACGACGACATCGAGCCCTACGCCCG
CGAGCGCGACGCACGGGCGGCAGAAGCACTCCGGGCCGCCGGGATCACCGTCCATGCCTGTGCCGAGATCTTGTTGCACC
CGGCCGGGGAGGTGCTCACCGCCGCGGGGGGCAAGCCCTACACGGTCTACACGCCCTTCTGGCGGCAATGGTCCGCCAAG
CCGAAGCCCAAGCCCTTCCCGACGCCTGAACGTCTCGAAGCGCCCACCGTTGAGGAGCAGTCTTTTCCGGAATTGGCGCA
ACTGGGCAGACCCTTCGCGGGCGAGCTGCTGGTCAGCCCGGGTGAGCAATCGGGGCTTGAGCAACTCGAAGCTTTTGCCC
GCGAAGGGCTCTACCGCTACGGCGAAAGGCGGGATCTGCCCGGTTGCGACGGCACTTCCCGCTTAAGTGCACACCTCAAA
TTCGGGACCGTTGGCATCCGGGCGGTCTGGGCGCGCACGATGGACGCCTGGAAGCAGGCCGAGCACGATCGCGACCGGGC
GGGACTGGCGGTCTGGCAGCAGGAACTGGGCTGGCGTGAATTTTACAAGTACGAGCTGTTTCACCTTCCGCAACTGGCCG
GTCGTCCCTTCCGGCGCGAGTTTGAGAATTTTCAGTGGGATGAGGACCAGGAGCGCTTCGAGCGCTGGTGCCAAGGAGAG
ACAGGCTATCCGATCGTCGATGCGGCGATGCGTCAGCTCAATACCGTCAGTTGGATGCACAACCGCCTGCGGATGATTGT
GGCCAGTTTTCTGACCAAAGATTTGCTGCTGCCCTACGGCTGGGGCGAGCGCTACTTCATGCAAAAACTGGTGGACGGCG
ATCTGTCGGCCAACAACGGCGGCTGGCAGTGGGCGGCCTCGGTGGGTACCGATCCCAAGCCTCTGCGGATTTTCAATCCG
AGTACCCAGGCGGGCCGCTACGACCCCAAAGCCGTTTTCATCCGCCGCTGGCTGCCTGAACTGGAGGGTGTCGACACGGC
GCTGTTGGTCACCTCCGAGCGCCTGCCGCCCCTGATGCGCGCCCAGCACCGCTACGCCCAGCCCATCGTCGAGCACAAGC
GCCAGCAGCAAGTTTTCAAGGAGCGCTACCGCGCCGTGCGCGCCCAGCAGGCGGATCCCGGCGCAAGGGACAGCGAGTGA

Upstream 100 bases:

>100_bases
ACCTTTCCCAGGATCACGAGTTTCAGATTGCTTACCATGTGCTCGATTTTTACGGCACCTGCGGCGAGTGCCGTCAGGCT
GTGGGAGCCTGAAGCGCACG

Downstream 100 bases:

>100_bases
CGGCTCACAAACGTTGGCGAATCTGCTACAACTGAAGCTAGTAGCAAGGAATATGTGCCATGGCGACCGCGTCGCCTGAG
CAGCACCAGCTTTTATGGCG

Product: DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSSIAIVWHRRDLRVHDNPALWQASRTGGQVLAVFIVDPTIVERDDTAPARIYFLRESVLELQKAYRTIGGRLAVRVGEP
VQQLVALAQAVGAGAVYFNDDIEPYARERDARAAEALRAAGITVHACAEILLHPAGEVLTAAGGKPYTVYTPFWRQWSAK
PKPKPFPTPERLEAPTVEEQSFPELAQLGRPFAGELLVSPGEQSGLEQLEAFAREGLYRYGERRDLPGCDGTSRLSAHLK
FGTVGIRAVWARTMDAWKQAEHDRDRAGLAVWQQELGWREFYKYELFHLPQLAGRPFRREFENFQWDEDQERFERWCQGE
TGYPIVDAAMRQLNTVSWMHNRLRMIVASFLTKDLLLPYGWGERYFMQKLVDGDLSANNGGWQWAASVGTDPKPLRIFNP
STQAGRYDPKAVFIRRWLPELEGVDTALLVTSERLPPLMRAQHRYAQPIVEHKRQQQVFKERYRAVRAQQADPGARDSE

Sequences:

>Translated_479_residues
MSSIAIVWHRRDLRVHDNPALWQASRTGGQVLAVFIVDPTIVERDDTAPARIYFLRESVLELQKAYRTIGGRLAVRVGEP
VQQLVALAQAVGAGAVYFNDDIEPYARERDARAAEALRAAGITVHACAEILLHPAGEVLTAAGGKPYTVYTPFWRQWSAK
PKPKPFPTPERLEAPTVEEQSFPELAQLGRPFAGELLVSPGEQSGLEQLEAFAREGLYRYGERRDLPGCDGTSRLSAHLK
FGTVGIRAVWARTMDAWKQAEHDRDRAGLAVWQQELGWREFYKYELFHLPQLAGRPFRREFENFQWDEDQERFERWCQGE
TGYPIVDAAMRQLNTVSWMHNRLRMIVASFLTKDLLLPYGWGERYFMQKLVDGDLSANNGGWQWAASVGTDPKPLRIFNP
STQAGRYDPKAVFIRRWLPELEGVDTALLVTSERLPPLMRAQHRYAQPIVEHKRQQQVFKERYRAVRAQQADPGARDSE
>Mature_478_residues
SSIAIVWHRRDLRVHDNPALWQASRTGGQVLAVFIVDPTIVERDDTAPARIYFLRESVLELQKAYRTIGGRLAVRVGEPV
QQLVALAQAVGAGAVYFNDDIEPYARERDARAAEALRAAGITVHACAEILLHPAGEVLTAAGGKPYTVYTPFWRQWSAKP
KPKPFPTPERLEAPTVEEQSFPELAQLGRPFAGELLVSPGEQSGLEQLEAFAREGLYRYGERRDLPGCDGTSRLSAHLKF
GTVGIRAVWARTMDAWKQAEHDRDRAGLAVWQQELGWREFYKYELFHLPQLAGRPFRREFENFQWDEDQERFERWCQGET
GYPIVDAAMRQLNTVSWMHNRLRMIVASFLTKDLLLPYGWGERYFMQKLVDGDLSANNGGWQWAASVGTDPKPLRIFNPS
TQAGRYDPKAVFIRRWLPELEGVDTALLVTSERLPPLMRAQHRYAQPIVEHKRQQQVFKERYRAVRAQQADPGARDSE

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=500, Percent_Identity=28.8, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI4758072, Length=503, Percent_Identity=27.4353876739563, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI188536103, Length=449, Percent_Identity=27.8396436525612, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1786926, Length=473, Percent_Identity=37.8435517970402, Blast_Score=274, Evalue=9e-75,
Organism=Saccharomyces cerevisiae, GI6324962, Length=506, Percent_Identity=27.0750988142292, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137248, Length=450, Percent_Identity=29.3333333333333, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24585455, Length=450, Percent_Identity=29.3333333333333, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24648152, Length=462, Percent_Identity=28.3549783549784, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54622; Mature: 54490

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIAIVWHRRDLRVHDNPALWQASRTGGQVLAVFIVDPTIVERDDTAPARIYFLRESVL
CCCEEEEEECCCCEECCCCCCEEECCCCCCEEEEEEECCEEECCCCCCCHHHHHHHHHHH
ELQKAYRTIGGRLAVRVGEPVQQLVALAQAVGAGAVYFNDDIEPYARERDARAAEALRAA
HHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHC
GITVHACAEILLHPAGEVLTAAGGKPYTVYTPFWRQWSAKPKPKPFPTPERLEAPTVEEQ
CCHHHHHHHHHHCCCCHHEECCCCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCCCCCC
SFPELAQLGRPFAGELLVSPGEQSGLEQLEAFAREGLYRYGERRDLPGCDGTSRLSAHLK
CCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEE
FGTVGIRAVWARTMDAWKQAEHDRDRAGLAVWQQELGWREFYKYELFHLPQLAGRPFRRE
ECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHCHHHCCCCHHHH
FENFQWDEDQERFERWCQGETGYPIVDAAMRQLNTVSWMHNRLRMIVASFLTKDLLLPYG
HHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WGERYFMQKLVDGDLSANNGGWQWAASVGTDPKPLRIFNPSTQAGRYDPKAVFIRRWLPE
CCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
LEGVDTALLVTSERLPPLMRAQHRYAQPIVEHKRQQQVFKERYRAVRAQQADPGARDSE
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSIAIVWHRRDLRVHDNPALWQASRTGGQVLAVFIVDPTIVERDDTAPARIYFLRESVL
CCEEEEEECCCCEECCCCCCEEECCCCCCEEEEEEECCEEECCCCCCCHHHHHHHHHHH
ELQKAYRTIGGRLAVRVGEPVQQLVALAQAVGAGAVYFNDDIEPYARERDARAAEALRAA
HHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHC
GITVHACAEILLHPAGEVLTAAGGKPYTVYTPFWRQWSAKPKPKPFPTPERLEAPTVEEQ
CCHHHHHHHHHHCCCCHHEECCCCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCCCCCC
SFPELAQLGRPFAGELLVSPGEQSGLEQLEAFAREGLYRYGERRDLPGCDGTSRLSAHLK
CCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEE
FGTVGIRAVWARTMDAWKQAEHDRDRAGLAVWQQELGWREFYKYELFHLPQLAGRPFRRE
ECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHCHHHCCCCHHHH
FENFQWDEDQERFERWCQGETGYPIVDAAMRQLNTVSWMHNRLRMIVASFLTKDLLLPYG
HHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WGERYFMQKLVDGDLSANNGGWQWAASVGTDPKPLRIFNPSTQAGRYDPKAVFIRRWLPE
CCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
LEGVDTALLVTSERLPPLMRAQHRYAQPIVEHKRQQQVFKERYRAVRAQQADPGARDSE
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]