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The map label for this gene is gca [H]

Identifier: 37520333

GI number: 37520333

Start: 817258

End: 818331

Strand: Reverse

Name: gca [H]

Synonym: gll0764

Alternate gene names: 37520333

Gene position: 818331-817258 (Counterclockwise)

Preceding gene: 37520334

Following gene: 37520332

Centisome position: 17.56

GC content: 64.99

Gene sequence:

>1074_bases
GTGCCTATCCTATCCACCGCACCGGGAAGCGACGGTCCCCGGCGGTCGTGTGGGAGCTTCGTCGGCGCTGGGGGGCAGCA
GGTGCGCGTCTTGATCACGGGAATCGGCGGGCAGGACGGGTTTTACTTGAGCCTGGCGCTGTTGCGCCGGGGCCACCGTG
TCTTTGGGATTCACCGCCGGGATCCGCCGGGTGAGCCGCTGGCAACGCTGCTGCCCGCCGGTGGGGATTTGCAGCTACTA
ACAGGCGATGTCACCGACCGCGCTTTTTTGCGGCGGGTGGTGGGCGAAGCGGCACCCGAGCAGATCTACAACCTGGCGGC
CCAGAGCCGCGTGGGCCAGAGTTTTGCGATGGCCGAGCGCACCTTCGAGGTCAATGCCCTGGCGCCGCTGTACCTGCTTG
AAATCATCCGCGAACTCAATCCGCGCATTCGCTTTTTCCAGGCCTGCTCCGCTGAGGTGTTCGGCGAATGCCCCCGAGCG
CCCCAGGACGAATCGACGCCTTTTGCGCCGGTGAGCCCCTACGCCATTGCCAAAGCGAGCGCCTACTGGCTGGTGAACAG
TTACCGGCGCTCCAGCGGTCTGTTTGCCTGCAACGCCATTCTCTACAACCACGAGTCGCCCCTGCGCGACGAGAGTTTTT
TGTCGGGCAAAGTCGTGCGCTCCCTGGCCCGCATTCTGGTGGGCCAGCAGCAGACGCTGGCGCTGGGCAACCTCGAAGCG
CGGCGCGATTGGGGCTTCGCGGGTGATTACGTCGAGGCGATGGTAGCGATGATGGCCCAGGCGGAGCCGGACGATTATGT
GATTGCCTCGGGTGAGGGGCACAGCGTCGGAGAATTCGTCGAGGAAGCCTTTGGCTACGCCCGTCTCGATTGGCGAAAAC
ACGTCGTTATCGACGAGCAGCTGTTGCGGGCGAGCGAGGCGCACGTGCTGTTGGGCAACGCCGGGCGCGCCCGGGCGCGG
CTCGGATGGCAGCCGCGCCTGGGATTTCAAGCGCTGGTGCGATTGATGGTAGACTCGGCCATTGAACAGGTGAAGGCCGC
CACCTTGAGTGCCGCCGCTTCCAGAGAACACTGA

Upstream 100 bases:

>100_bases
GACAGTGCCGACAAGTACCTTTCGGAGCGCTTCTGGGACGACCCGAATCTATAATCCGCCCGAAGCGTTGACCGCCGGCC
TCCGAGCGCCGAAGATAAGG

Downstream 100 bases:

>100_bases
CAGGAGAAACATGAGCGAATCCAAGCGCGCCTTGATCACTGGGATCACCGGTCAGGACGGATCTTACCTGGCTGAATATC
TGCTTGGCTGCGGCTACGAA

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPILSTAPGSDGPRRSCGSFVGAGGQQVRVLITGIGGQDGFYLSLALLRRGHRVFGIHRRDPPGEPLATLLPAGGDLQLL
TGDVTDRAFLRRVVGEAAPEQIYNLAAQSRVGQSFAMAERTFEVNALAPLYLLEIIRELNPRIRFFQACSAEVFGECPRA
PQDESTPFAPVSPYAIAKASAYWLVNSYRRSSGLFACNAILYNHESPLRDESFLSGKVVRSLARILVGQQQTLALGNLEA
RRDWGFAGDYVEAMVAMMAQAEPDDYVIASGEGHSVGEFVEEAFGYARLDWRKHVVIDEQLLRASEAHVLLGNAGRARAR
LGWQPRLGFQALVRLMVDSAIEQVKAATLSAAASREH

Sequences:

>Translated_357_residues
MPILSTAPGSDGPRRSCGSFVGAGGQQVRVLITGIGGQDGFYLSLALLRRGHRVFGIHRRDPPGEPLATLLPAGGDLQLL
TGDVTDRAFLRRVVGEAAPEQIYNLAAQSRVGQSFAMAERTFEVNALAPLYLLEIIRELNPRIRFFQACSAEVFGECPRA
PQDESTPFAPVSPYAIAKASAYWLVNSYRRSSGLFACNAILYNHESPLRDESFLSGKVVRSLARILVGQQQTLALGNLEA
RRDWGFAGDYVEAMVAMMAQAEPDDYVIASGEGHSVGEFVEEAFGYARLDWRKHVVIDEQLLRASEAHVLLGNAGRARAR
LGWQPRLGFQALVRLMVDSAIEQVKAATLSAAASREH
>Mature_356_residues
PILSTAPGSDGPRRSCGSFVGAGGQQVRVLITGIGGQDGFYLSLALLRRGHRVFGIHRRDPPGEPLATLLPAGGDLQLLT
GDVTDRAFLRRVVGEAAPEQIYNLAAQSRVGQSFAMAERTFEVNALAPLYLLEIIRELNPRIRFFQACSAEVFGECPRAP
QDESTPFAPVSPYAIAKASAYWLVNSYRRSSGLFACNAILYNHESPLRDESFLSGKVVRSLARILVGQQQTLALGNLEAR
RDWGFAGDYVEAMVAMMAQAEPDDYVIASGEGHSVGEFVEEAFGYARLDWRKHVVIDEQLLRASEAHVLLGNAGRARARL
GWQPRLGFQALVRLMVDSAIEQVKAATLSAAASREH

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=41.4492753623188, Blast_Score=272, Evalue=3e-73,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=38.0952380952381, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI48994969, Length=206, Percent_Identity=27.6699029126214, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788353, Length=293, Percent_Identity=22.8668941979522, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786974, Length=280, Percent_Identity=24.2857142857143, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=41.8604651162791, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=41.5204678362573, Blast_Score=258, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=41.5204678362573, Blast_Score=258, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=41.5204678362573, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=41.5204678362573, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=41.5204678362573, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24158427, Length=364, Percent_Identity=40.6593406593407, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38910; Mature: 38779

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILSTAPGSDGPRRSCGSFVGAGGQQVRVLITGIGGQDGFYLSLALLRRGHRVFGIHRR
CCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECC
DPPGEPLATLLPAGGDLQLLTGDVTDRAFLRRVVGEAAPEQIYNLAAQSRVGQSFAMAER
CCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHH
TFEVNALAPLYLLEIIRELNPRIRFFQACSAEVFGECPRAPQDESTPFAPVSPYAIAKAS
HEECCCHHHHHHHHHHHHHCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
AYWLVNSYRRSSGLFACNAILYNHESPLRDESFLSGKVVRSLARILVGQQQTLALGNLEA
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCH
RRDWGFAGDYVEAMVAMMAQAEPDDYVIASGEGHSVGEFVEEAFGYARLDWRKHVVIDEQ
HCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCEEECCCHHEEHHHH
LLRASEAHVLLGNAGRARARLGWQPRLGFQALVRLMVDSAIEQVKAATLSAAASREH
HHHCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PILSTAPGSDGPRRSCGSFVGAGGQQVRVLITGIGGQDGFYLSLALLRRGHRVFGIHRR
CCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECC
DPPGEPLATLLPAGGDLQLLTGDVTDRAFLRRVVGEAAPEQIYNLAAQSRVGQSFAMAER
CCCCCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHH
TFEVNALAPLYLLEIIRELNPRIRFFQACSAEVFGECPRAPQDESTPFAPVSPYAIAKAS
HEECCCHHHHHHHHHHHHHCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
AYWLVNSYRRSSGLFACNAILYNHESPLRDESFLSGKVVRSLARILVGQQQTLALGNLEA
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCH
RRDWGFAGDYVEAMVAMMAQAEPDDYVIASGEGHSVGEFVEEAFGYARLDWRKHVVIDEQ
HCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCEEECCCHHEEHHHH
LLRASEAHVLLGNAGRARARLGWQPRLGFQALVRLMVDSAIEQVKAATLSAAASREH
HHHCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]