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The map label for this gene is ndhJ

Identifier: 37520319

GI number: 37520319

Start: 800181

End: 800711

Strand: Direct

Name: ndhJ

Synonym: gvip082

Alternate gene names: 37520319

Gene position: 800181-800711 (Clockwise)

Preceding gene: 37520318

Following gene: 37520320

Centisome position: 17.17

GC content: 60.83

Gene sequence:

>531_bases
ATGGAAGAGCAAACCACCCAATCCGCGGCCGATGGGCAGACTGCCATCGAGCTTGTCACCGGTCCTATTTCCGACGCGCT
CAAGGCGCGCGGTTTACCCCACGAGTTGACGGGTCTCGACAACCGCAAAATCGAGATCATCAAAGTGGAGCCCGAGCACC
TGATCGCGGTGGCCAGGGCTCTCTACGACGACGGCTTCAACTATCTGGCCTGCCAGTGCGGCTTCGACGAAGGCCCTGGT
GATTCCCTCGGGAGCATGTACCACCTCACCAAGCTGAGCGACAGTGCCGATCGGCCGCCGGAAGTGCGCATCAAAGTCTT
TCTGCCCCGCGACAACCCGCGCGTCCCTTCGGTCTACTGGATCTGGAAGACGGCGGACTGGCAGGAGCGCGAGTCGTTCG
ACATGTACGGGATCATCTACGAAGGCCATCCCAACCTCATCCGCATCCTGATGCCGGAGGACTGGGTGGGCTGGCCGATG
CGCAAAGATTACGTGACGCCCGACTTTTACGAACTGCAGGACGCCTACTGA

Upstream 100 bases:

>100_bases
AGCCCGACCCGCCAGGCGCCCCCACCGGGGCTGGCGGCGGCCATGGAGATGCCTCTGGTGCTGCCCGAAGTCGTCAAGCA
AAACGAACAGGTCTGATCCG

Downstream 100 bases:

>100_bases
GCCCTACACGGTCTATGGCACGACCGGGTGGGTGGTCGTGAGGTGTTGGCTGGTATCGGTAAGGCTTTTGCTCGCCTGCA
ACGGATTTGGGCGGCTCAAG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MEEQTTQSAADGQTAIELVTGPISDALKARGLPHELTGLDNRKIEIIKVEPEHLIAVARALYDDGFNYLACQCGFDEGPG
DSLGSMYHLTKLSDSADRPPEVRIKVFLPRDNPRVPSVYWIWKTADWQERESFDMYGIIYEGHPNLIRILMPEDWVGWPM
RKDYVTPDFYELQDAY

Sequences:

>Translated_176_residues
MEEQTTQSAADGQTAIELVTGPISDALKARGLPHELTGLDNRKIEIIKVEPEHLIAVARALYDDGFNYLACQCGFDEGPG
DSLGSMYHLTKLSDSADRPPEVRIKVFLPRDNPRVPSVYWIWKTADWQERESFDMYGIIYEGHPNLIRILMPEDWVGWPM
RKDYVTPDFYELQDAY
>Mature_176_residues
MEEQTTQSAADGQTAIELVTGPISDALKARGLPHELTGLDNRKIEIIKVEPEHLIAVARALYDDGFNYLACQCGFDEGPG
DSLGSMYHLTKLSDSADRPPEVRIKVFLPRDNPRVPSVYWIWKTADWQERESFDMYGIIYEGHPNLIRILMPEDWVGWPM
RKDYVTPDFYELQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family

Homologues:

Organism=Escherichia coli, GI145693162, Length=86, Percent_Identity=41.8604651162791, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHJ_GLOVI (Q7NML5)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923696.1
- ProteinModelPortal:   Q7NML5
- SMR:   Q7NML5
- GeneID:   2599369
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.750
- HOGENOM:   HBG727675
- OMA:   YELQDAY
- ProtClustDB:   PRK12494
- BioCyc:   GVIO251221:GLR0750-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01357
- InterPro:   IPR010218
- InterPro:   IPR001268
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa

EC number: 1.6.5.3

Molecular weight: Translated: 20151; Mature: 20151

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEQTTQSAADGQTAIELVTGPISDALKARGLPHELTGLDNRKIEIIKVEPEHLIAVARA
CCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHCCCCCCEEEEEEECHHHHHHHHHH
LYDDGFNYLACQCGFDEGPGDSLGSMYHLTKLSDSADRPPEVRIKVFLPRDNPRVPSVYW
HHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCEEEE
IWKTADWQERESFDMYGIIYEGHPNLIRILMPEDWVGWPMRKDYVTPDFYELQDAY
EEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MEEQTTQSAADGQTAIELVTGPISDALKARGLPHELTGLDNRKIEIIKVEPEHLIAVARA
CCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHCCCCCCEEEEEEECHHHHHHHHHH
LYDDGFNYLACQCGFDEGPGDSLGSMYHLTKLSDSADRPPEVRIKVFLPRDNPRVPSVYW
HHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCEEEE
IWKTADWQERESFDMYGIIYEGHPNLIRILMPEDWVGWPMRKDYVTPDFYELQDAY
EEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14621292