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The map label for this gene is ndhK

Identifier: 37520318

GI number: 37520318

Start: 799478

End: 800176

Strand: Direct

Name: ndhK

Synonym: gvip081

Alternate gene names: 37520318

Gene position: 799478-800176 (Clockwise)

Preceding gene: 37520317

Following gene: 37520319

Centisome position: 17.16

GC content: 62.66

Gene sequence:

>699_bases
ATGGACATCAAGAACCTGCTCAACCCGATCGAGCGCCCCGAAGTCACCAGCGAACTGACCAACAACGTGGTGCTCACGAC
GCTCAACGATCTGTACAACTGGGCGCGGCTTTCGAGCGTCTGGCCTCTGATGTACGGCACGAGCTGCTGCTTTATCGAAT
TTGCCGGACTGATCGGCTCGCGCTTCGACTTCGACCGCTTCGGGCTGGTGCCCCGCGCCTCACCGCGCCAGGCGGACCTG
ATCATCACCGCCGGTACGGTCACCATGAAGATGGCCCCGGCGCTGGTGACCCTCTACGAACAGATGCCCGAGCCCAAGTA
CGTGATCGCCATGGGCGCGTGCACGATCACCGGCGGTATGTTCTCCACCGACTCCTACACCACCGTGCGGGGTGTCGATA
AGCTCATACCGGTGGACGTGTACATTCCGGGTTGCCCGCCCAGGCCCGAGGCGATCTTCGATGCGATCGTCAAGTTGCGC
AAGAAAATGGCCACCGAGGACGTGCGCGACCGCTACGAGCAAATCCGCCAGACCCATCGCTACCACACTACTGCCCACAC
GCTCAAAGCGGTCCCGGAGTCACTCTCGAGCAAGTATCTCGAGAGCCCGACCCGCCAGGCGCCCCCACCGGGGCTGGCGG
CGGCCATGGAGATGCCTCTGGTGCTGCCCGAAGTCGTCAAGCAAAACGAACAGGTCTGA

Upstream 100 bases:

>100_bases
GTCTACGCCTGGCGCAAGGGCGCCCTCGAGTGGACCTGATTCGACCCGACTGTATATTCTGCATCCACCCACCCCGCACG
GGCGCAAGCCCCAAGACGCC

Downstream 100 bases:

>100_bases
TCCGATGGAAGAGCAAACCACCCAATCCGCGGCCGATGGGCAGACTGCCATCGAGCTTGTCACCGGTCCTATTTCCGACG
CGCTCAAGGCGCGCGGTTTA

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K 1; NDH-1 subunit K 1; NDH-K 1

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MDIKNLLNPIERPEVTSELTNNVVLTTLNDLYNWARLSSVWPLMYGTSCCFIEFAGLIGSRFDFDRFGLVPRASPRQADL
IITAGTVTMKMAPALVTLYEQMPEPKYVIAMGACTITGGMFSTDSYTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIVKLR
KKMATEDVRDRYEQIRQTHRYHTTAHTLKAVPESLSSKYLESPTRQAPPPGLAAAMEMPLVLPEVVKQNEQV

Sequences:

>Translated_232_residues
MDIKNLLNPIERPEVTSELTNNVVLTTLNDLYNWARLSSVWPLMYGTSCCFIEFAGLIGSRFDFDRFGLVPRASPRQADL
IITAGTVTMKMAPALVTLYEQMPEPKYVIAMGACTITGGMFSTDSYTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIVKLR
KKMATEDVRDRYEQIRQTHRYHTTAHTLKAVPESLSSKYLESPTRQAPPPGLAAAMEMPLVLPEVVKQNEQV
>Mature_232_residues
MDIKNLLNPIERPEVTSELTNNVVLTTLNDLYNWARLSSVWPLMYGTSCCFIEFAGLIGSRFDFDRFGLVPRASPRQADL
IITAGTVTMKMAPALVTLYEQMPEPKYVIAMGACTITGGMFSTDSYTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIVKLR
KKMATEDVRDRYEQIRQTHRYHTTAHTLKAVPESLSSKYLESPTRQAPPPGLAAAMEMPLVLPEVVKQNEQV

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=53.1468531468531, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1788624, Length=164, Percent_Identity=50, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1788834, Length=169, Percent_Identity=34.3195266272189, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=43.3962264150943, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=158, Percent_Identity=48.1012658227848, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=53.9568345323741, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=53.9568345323741, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=52.9411764705882, Blast_Score=167, Evalue=5e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK1_GLOVI (Q7NML6)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923695.1
- ProteinModelPortal:   Q7NML6
- SMR:   Q7NML6
- GeneID:   2599368
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.749
- HOGENOM:   HBG553221
- OMA:   MAANMAR
- ProtClustDB:   PRK06411
- BioCyc:   GVIO251221:GLR0749-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 25910; Mature: 25910

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKNLLNPIERPEVTSELTNNVVLTTLNDLYNWARLSSVWPLMYGTSCCFIEFAGLIGS
CCHHHHHCHHCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
RFDFDRFGLVPRASPRQADLIITAGTVTMKMAPALVTLYEQMPEPKYVIAMGACTITGGM
CCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECHHEEECCC
FSTDSYTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIVKLRKKMATEDVRDRYEQIRQTHR
CCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHTTAHTLKAVPESLSSKYLESPTRQAPPPGLAAAMEMPLVLPEVVKQNEQV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDIKNLLNPIERPEVTSELTNNVVLTTLNDLYNWARLSSVWPLMYGTSCCFIEFAGLIGS
CCHHHHHCHHCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
RFDFDRFGLVPRASPRQADLIITAGTVTMKMAPALVTLYEQMPEPKYVIAMGACTITGGM
CCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECHHEEECCC
FSTDSYTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIVKLRKKMATEDVRDRYEQIRQTHR
CCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YHTTAHTLKAVPESLSSKYLESPTRQAPPPGLAAAMEMPLVLPEVVKQNEQV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14621292