Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 37520296

GI number: 37520296

Start: 775813

End: 777819

Strand: Direct

Name: ligA

Synonym: glr0727

Alternate gene names: 37520296

Gene position: 775813-777819 (Clockwise)

Preceding gene: 37520293

Following gene: 37520297

Centisome position: 16.65

GC content: 65.72

Gene sequence:

>2007_bases
ATGTCCACAACCGTCCCCCCCGAGATCGAGGAGCACACCCGGACGTTGCGCGCGCTTTTGCACCGGTGGGGCTACGCCTA
TTATGTGCTGGATGCTCCCGAGGTGAGCGACGCCATCTACGACCAGCACTACCGCGAACTGGTCGACCTCGAAAGCCGTT
ATCCGGAGCTTGTCAGCCCCGATTCACCCACCCGGCGCGTCGGGGAGCGCCCCGCCTCGGCCTTTGTGAGTGTCACCCAC
CGGGTGCCGATGTTCTCCCTCGAGAACGCCTTCAGCCAGGCGGAACTCGAAAAATGGGGCGAAAGGCTGCTGCGTGCAAT
CGGCCCGGGTCTGGAGTTTATCTGCGAACTGAAAATCGACGGATCTGCCACTGCCTTGAGTTACGAGGACGGAGTGCTGG
TGCGCGGCGCCACCCGCGGCGACGGCGTCGAGGGTGAAGAAATTACCCAGAACCTGCGCACGATCCGGGCGATTCCTCTC
AAGCTGCTGGGGGGCGAGGTACCGGCGGTGCTGGAGGTGCGCGGCGAGGCGTTCATACCCCGCGACGAATTTGAGCGCAT
CAACCAGGAGCGCCAGGCGGCCGGTGAAAAACTGTTCGCCAACCCGCGAAACGCCTGCGCCGGCACCCTCAGACAACTCG
ATTCGCGGGTGGTGGCCTCGCGCCGCCTGGGCTTTTTTGCCTACACCGCCCACTACGGCCGCGCCGAAAGCCAGTGGGAA
GCGCTCGCAGAACTCGAAAGCCACGGCTTTCGGGTCAACCCCCACCGCAGTCTGTGCCGGGATCTGGCCGAGGTGCGGAC
GTTTTGTGAGCACTGGGAGAACCACCGCCACGAGTTGCCCTACGACACCGACGGCGTGGTGGTCAAAGTCAATGCCTTTG
ACCATCAGCGGGAGGTGGGTTTCACCAGCAAATTTCCGCGCTGGGCGATTGCCTTCAAGTACCCTGCCGAAGAAAAAAGC
ACCGTCGTCGAAGCGATTGCCGTCCAGGTCGGGCGCACCGGGGCGCTCACGCCGGTGGCCGAACTGCAGCCGGTGGCGGT
GGCAGGGACGACGGTCAGCCGGGCGACCTTGCACAACCAGGATCGTATCGAGTCTCTCGATGTGCGGGTCGGAGACACCG
TAATTATTCGCAAGGCGGGCGAGATTATCCCGGAGGTGGTACGGGTGATCGGCGAATTGCGCCCGCCCGAAGCCGTGCCC
TACGTTTTTCCCCAGACCTGTCCGGAGTGCGGCACGGCGGTAGTGCGCGCCCCGGGCGAGGCGGCGGTGCGCTGTCCAAA
CCCCCGGTGCCCGGCGCTCATCCGGGGGAAGCTCGGCCACTGGTGCGCGGCCCTCGAAATCGACGGCATCGGCGACAAGC
TCATCGCCAGGCTGGTAAGCCTCGGCCTGGTCCATACGGTGGCGGATCTGTACGAACTGAGCGCAGAGCAACTGGCGGGC
CTGGAGCGTCTGGGCGCCCGTTCGGCCGCCAAGATTGTCGAGCAACTGGACCGCTCGCACCGCCAGCCCTGGAGCCGGGT
GCTCTATGGCCTGGGGCTGCGCCACATCGGTGCGAGCGTCTCGGTGGAGTTGGCCCGCGCTTTCGCAAGCGCCGATGCTC
TCGCCCGAGCCGACCTGGCTGCTATCGCTTCGCTCTACGGCTTTGGAGAGGAGTTGGCCCGCTCGGTGGTCGAGTGGTTC
GCGCAAGCAGAAAACCGCGCACTGCTCGAACGGCTCAAAGCCCACGGTCTACAGCTGGCCGGCGGCGGGCGGGCCGCCCA
GAGCAGTGCCCTGGCGGGGCTCACCTTCGTGATCACCGGTACGCTGCCGACGCTGTCGCGCGAGGAGTGCACCGCCCTCA
TCGAGAGCCACGGCGGCAAGGTGACCAGTTCGGTGAGCAGCCGTACCAGCTACGTGGTGGCGGGTGAAAAAGCCGGCTCC
AAACTCGCCCGTGCCCAGGATCTGAAGGTGGCCGTCCTGGATGAGGAGCAATTGCGGGCGCTCATCGAGACGCGTGAGAT
GCCGTGA

Upstream 100 bases:

>100_bases
CCAGGCACACCGAAAAGATATCTCTAAGCGCGACATCCGGGGAAGGCTCCCCACAACCCCGGGCGCGTAAAATCTGGAAA
GGCCCGCGCGAGGTAACCTG

Downstream 100 bases:

>100_bases
TCCTAGTTGACGACGTCTGGGAACCGCCCGCTGTCGGTATGCTGAGAAGATGCCGAGCGTGCCTCCCGTTCCGGCTCCAC
AATCGACATGAAGCAATTGC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MSTTVPPEIEEHTRTLRALLHRWGYAYYVLDAPEVSDAIYDQHYRELVDLESRYPELVSPDSPTRRVGERPASAFVSVTH
RVPMFSLENAFSQAELEKWGERLLRAIGPGLEFICELKIDGSATALSYEDGVLVRGATRGDGVEGEEITQNLRTIRAIPL
KLLGGEVPAVLEVRGEAFIPRDEFERINQERQAAGEKLFANPRNACAGTLRQLDSRVVASRRLGFFAYTAHYGRAESQWE
ALAELESHGFRVNPHRSLCRDLAEVRTFCEHWENHRHELPYDTDGVVVKVNAFDHQREVGFTSKFPRWAIAFKYPAEEKS
TVVEAIAVQVGRTGALTPVAELQPVAVAGTTVSRATLHNQDRIESLDVRVGDTVIIRKAGEIIPEVVRVIGELRPPEAVP
YVFPQTCPECGTAVVRAPGEAAVRCPNPRCPALIRGKLGHWCAALEIDGIGDKLIARLVSLGLVHTVADLYELSAEQLAG
LERLGARSAAKIVEQLDRSHRQPWSRVLYGLGLRHIGASVSVELARAFASADALARADLAAIASLYGFGEELARSVVEWF
AQAENRALLERLKAHGLQLAGGGRAAQSSALAGLTFVITGTLPTLSREECTALIESHGGKVTSSVSSRTSYVVAGEKAGS
KLARAQDLKVAVLDEEQLRALIETREMP

Sequences:

>Translated_668_residues
MSTTVPPEIEEHTRTLRALLHRWGYAYYVLDAPEVSDAIYDQHYRELVDLESRYPELVSPDSPTRRVGERPASAFVSVTH
RVPMFSLENAFSQAELEKWGERLLRAIGPGLEFICELKIDGSATALSYEDGVLVRGATRGDGVEGEEITQNLRTIRAIPL
KLLGGEVPAVLEVRGEAFIPRDEFERINQERQAAGEKLFANPRNACAGTLRQLDSRVVASRRLGFFAYTAHYGRAESQWE
ALAELESHGFRVNPHRSLCRDLAEVRTFCEHWENHRHELPYDTDGVVVKVNAFDHQREVGFTSKFPRWAIAFKYPAEEKS
TVVEAIAVQVGRTGALTPVAELQPVAVAGTTVSRATLHNQDRIESLDVRVGDTVIIRKAGEIIPEVVRVIGELRPPEAVP
YVFPQTCPECGTAVVRAPGEAAVRCPNPRCPALIRGKLGHWCAALEIDGIGDKLIARLVSLGLVHTVADLYELSAEQLAG
LERLGARSAAKIVEQLDRSHRQPWSRVLYGLGLRHIGASVSVELARAFASADALARADLAAIASLYGFGEELARSVVEWF
AQAENRALLERLKAHGLQLAGGGRAAQSSALAGLTFVITGTLPTLSREECTALIESHGGKVTSSVSSRTSYVVAGEKAGS
KLARAQDLKVAVLDEEQLRALIETREMP
>Mature_667_residues
STTVPPEIEEHTRTLRALLHRWGYAYYVLDAPEVSDAIYDQHYRELVDLESRYPELVSPDSPTRRVGERPASAFVSVTHR
VPMFSLENAFSQAELEKWGERLLRAIGPGLEFICELKIDGSATALSYEDGVLVRGATRGDGVEGEEITQNLRTIRAIPLK
LLGGEVPAVLEVRGEAFIPRDEFERINQERQAAGEKLFANPRNACAGTLRQLDSRVVASRRLGFFAYTAHYGRAESQWEA
LAELESHGFRVNPHRSLCRDLAEVRTFCEHWENHRHELPYDTDGVVVKVNAFDHQREVGFTSKFPRWAIAFKYPAEEKST
VVEAIAVQVGRTGALTPVAELQPVAVAGTTVSRATLHNQDRIESLDVRVGDTVIIRKAGEIIPEVVRVIGELRPPEAVPY
VFPQTCPECGTAVVRAPGEAAVRCPNPRCPALIRGKLGHWCAALEIDGIGDKLIARLVSLGLVHTVADLYELSAEQLAGL
ERLGARSAAKIVEQLDRSHRQPWSRVLYGLGLRHIGASVSVELARAFASADALARADLAAIASLYGFGEELARSVVEWFA
QAENRALLERLKAHGLQLAGGGRAAQSSALAGLTFVITGTLPTLSREECTALIESHGGKVTSSVSSRTSYVVAGEKAGSK
LARAQDLKVAVLDEEQLRALIETREMP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=81, Percent_Identity=46.9135802469136, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=44.8736998514116, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI87082305, Length=581, Percent_Identity=25.8175559380379, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324791, Length=78, Percent_Identity=46.1538461538462, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI62472391, Length=76, Percent_Identity=44.7368421052632, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17737765, Length=76, Percent_Identity=44.7368421052632, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_GLOVI (Q7NMN8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923673.1
- HSSP:   O87703
- ProteinModelPortal:   Q7NMN8
- GeneID:   2599446
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr0727
- NMPDR:   fig|251221.1.peg.727
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   GVIO251221:GLR0727-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73225; Mature: 73094

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 176-176 BINDING 289-289 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTVPPEIEEHTRTLRALLHRWGYAYYVLDAPEVSDAIYDQHYRELVDLESRYPELVSP
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DSPTRRVGERPASAFVSVTHRVPMFSLENAFSQAELEKWGERLLRAIGPGLEFICELKID
CCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
GSATALSYEDGVLVRGATRGDGVEGEEITQNLRTIRAIPLKLLGGEVPAVLEVRGEAFIP
CCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
RDEFERINQERQAAGEKLFANPRNACAGTLRQLDSRVVASRRLGFFAYTAHYGRAESQWE
HHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCHHHHH
ALAELESHGFRVNPHRSLCRDLAEVRTFCEHWENHRHELPYDTDGVVVKVNAFDHQREVG
HHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHCC
FTSKFPRWAIAFKYPAEEKSTVVEAIAVQVGRTGALTPVAELQPVAVAGTTVSRATLHNQ
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCHHHHHHCCC
DRIESLDVRVGDTVIIRKAGEIIPEVVRVIGELRPPEAVPYVFPQTCPECGTAVVRAPGE
HHHHHHCEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCEEECCCC
AAVRCPNPRCPALIRGKLGHWCAALEIDGIGDKLIARLVSLGLVHTVADLYELSAEQLAG
CCEECCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERLGARSAAKIVEQLDRSHRQPWSRVLYGLGLRHIGASVSVELARAFASADALARADLA
HHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AIASLYGFGEELARSVVEWFAQAENRALLERLKAHGLQLAGGGRAAQSSALAGLTFVITG
HHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCCHHHHHHHCCEEEEEEC
TLPTLSREECTALIESHGGKVTSSVSSRTSYVVAGEKAGSKLARAQDLKVAVLDEEQLRA
CCCCCCHHHHHHHHHHCCCCEEHHHHCCCEEEEECCHHCHHHHHHCCCEEEEECHHHHHH
LIETREMP
HHHHCCCC
>Mature Secondary Structure 
STTVPPEIEEHTRTLRALLHRWGYAYYVLDAPEVSDAIYDQHYRELVDLESRYPELVSP
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DSPTRRVGERPASAFVSVTHRVPMFSLENAFSQAELEKWGERLLRAIGPGLEFICELKID
CCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
GSATALSYEDGVLVRGATRGDGVEGEEITQNLRTIRAIPLKLLGGEVPAVLEVRGEAFIP
CCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
RDEFERINQERQAAGEKLFANPRNACAGTLRQLDSRVVASRRLGFFAYTAHYGRAESQWE
HHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCHHHHH
ALAELESHGFRVNPHRSLCRDLAEVRTFCEHWENHRHELPYDTDGVVVKVNAFDHQREVG
HHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHCC
FTSKFPRWAIAFKYPAEEKSTVVEAIAVQVGRTGALTPVAELQPVAVAGTTVSRATLHNQ
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCHHHHHHCCC
DRIESLDVRVGDTVIIRKAGEIIPEVVRVIGELRPPEAVPYVFPQTCPECGTAVVRAPGE
HHHHHHCEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCEEECCCC
AAVRCPNPRCPALIRGKLGHWCAALEIDGIGDKLIARLVSLGLVHTVADLYELSAEQLAG
CCEECCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERLGARSAAKIVEQLDRSHRQPWSRVLYGLGLRHIGASVSVELARAFASADALARADLA
HHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AIASLYGFGEELARSVVEWFAQAENRALLERLKAHGLQLAGGGRAAQSSALAGLTFVITG
HHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCCHHHHHHHCCEEEEEEC
TLPTLSREECTALIESHGGKVTSSVSSRTSYVVAGEKAGSKLARAQDLKVAVLDEEQLRA
CCCCCCHHHHHHHHHHCCCCEEHHHHCCCEEEEECCHHCHHHHHHCCCEEEEECHHHHHH
LIETREMP
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292