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The map label for this gene is gacS [H]

Identifier: 37520141

GI number: 37520141

Start: 609544

End: 610656

Strand: Reverse

Name: gacS [H]

Synonym: gll0572

Alternate gene names: 37520141

Gene position: 610656-609544 (Counterclockwise)

Preceding gene: 37520147

Following gene: 37520140

Centisome position: 13.11

GC content: 64.6

Gene sequence:

>1113_bases
ATGCCAGCCCGCGACAGGTACCAGATGCTCCGGCGCAGCTTTTCTTTTCGACCGATTGGCGCGTACATCGCCCTGATGTT
TGCCGTCATCTTGGTGCTGGAGTTTTCGACGCCGCCGGAGTACATCTTCGGCTATCTTTACGTCGGGCCGATTTTGCTGG
CCAACTGGTTTCTCGACCGGCGCAGCACCGTGTTCGTCACCAGCCTGGGGGTTGTGCTGACCCTGGCCAACCTGCTTTTT
CCCCGCGACGCGCTGGCCAGCAGCGTCGCGGTGGTCGATCGGTCGATCACGGTGGCGGCCCTGCTCACCGTCGCCTACCT
GGGGACTCAGTACCGGCGCATCCAGGAAGAGTCCGCCCGCCAGAAGGCGGCCCTGCAGGCCAGAGAAGAACTCTCCCGGG
CACAATCGGATTTTGTGGCTACCCTCACCCACGATTTGAAGACCCCCCTGTTGGGCGCCCAGCAGACGCTGCAATTTTTT
GGCCGCGGCCAGTTCGGCGAGGTCAGCCCCCAACAGCTGGGGGTGATCGAAATGCTGCAAAAAAGCAATCACAAGCTGCT
CGCCCTGGTGGACACGCTGCTTGCTATCTATCGCAACAGCCTGTCGGGCCTGCCGCTCGCAAAGCAGCCGAGCGATCTCG
ACGAGTTGTGCGCCGAGCAGATCGTGGTGCTGCAAGATCTGGCCGTAAGCCGGCAGATCGAACTGGTGTACGAAGGCTCC
GAAGGAGCACACCTGCCGGTGGACGCTCTGCAACTGGGCCGGGTGGTGGCCAATCTGGTGGGTAACGCCATCAACCACAC
TCCCCGGGGCGGCCGGGTGACGGTGCGATTGTTGCGGGCGCCGGACGAGTGGCGGCTTTTGGTCGAGGACACCGGCACGG
GCATTCCGCCCACGGATATCCGGCGGGTCTTCGAGCGCTTCTACCAAGCCGAAAATACCCGCCACGTCCCTGGGACCGGC
CTGGGGCTGTACCTGTCCCGGCAAATCGTCGAAGCCCACGGCGGCCGGATCTGGGCGGCGAACCGGCCGGGGGGCGGTTG
TCTGTTTGGGGTGAGCCTGCCCGACTCGGTGCCGCCGGGTGCCGCAGCCCTCGCCCGGAGCGCTGCAAGATGA

Upstream 100 bases:

>100_bases
GAACCATGGGTATATAACGGTTGATATAGACCGGCCGCGGCGAGCGAATCCAACTGAATCATCGAACCGAGCATTTGTTA
AGCTGAAGGCAATACCGGCG

Downstream 100 bases:

>100_bases
GCGGGAGGGTGCGTGTGCTACTCGTCGAGGATGACCCGCTTTTTCGGATGGGCCTCGCCATGGTCCTGCGCCACCAGCCG
CAGATCGAGCTGGTGGCCGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPARDRYQMLRRSFSFRPIGAYIALMFAVILVLEFSTPPEYIFGYLYVGPILLANWFLDRRSTVFVTSLGVVLTLANLLF
PRDALASSVAVVDRSITVAALLTVAYLGTQYRRIQEESARQKAALQAREELSRAQSDFVATLTHDLKTPLLGAQQTLQFF
GRGQFGEVSPQQLGVIEMLQKSNHKLLALVDTLLAIYRNSLSGLPLAKQPSDLDELCAEQIVVLQDLAVSRQIELVYEGS
EGAHLPVDALQLGRVVANLVGNAINHTPRGGRVTVRLLRAPDEWRLLVEDTGTGIPPTDIRRVFERFYQAENTRHVPGTG
LGLYLSRQIVEAHGGRIWAANRPGGGCLFGVSLPDSVPPGAAALARSAAR

Sequences:

>Translated_370_residues
MPARDRYQMLRRSFSFRPIGAYIALMFAVILVLEFSTPPEYIFGYLYVGPILLANWFLDRRSTVFVTSLGVVLTLANLLF
PRDALASSVAVVDRSITVAALLTVAYLGTQYRRIQEESARQKAALQAREELSRAQSDFVATLTHDLKTPLLGAQQTLQFF
GRGQFGEVSPQQLGVIEMLQKSNHKLLALVDTLLAIYRNSLSGLPLAKQPSDLDELCAEQIVVLQDLAVSRQIELVYEGS
EGAHLPVDALQLGRVVANLVGNAINHTPRGGRVTVRLLRAPDEWRLLVEDTGTGIPPTDIRRVFERFYQAENTRHVPGTG
LGLYLSRQIVEAHGGRIWAANRPGGGCLFGVSLPDSVPPGAAALARSAAR
>Mature_369_residues
PARDRYQMLRRSFSFRPIGAYIALMFAVILVLEFSTPPEYIFGYLYVGPILLANWFLDRRSTVFVTSLGVVLTLANLLFP
RDALASSVAVVDRSITVAALLTVAYLGTQYRRIQEESARQKAALQAREELSRAQSDFVATLTHDLKTPLLGAQQTLQFFG
RGQFGEVSPQQLGVIEMLQKSNHKLLALVDTLLAIYRNSLSGLPLAKQPSDLDELCAEQIVVLQDLAVSRQIELVYEGSE
GAHLPVDALQLGRVVANLVGNAINHTPRGGRVTVRLLRAPDEWRLLVEDTGTGIPPTDIRRVFERFYQAENTRHVPGTGL
GLYLSRQIVEAHGGRIWAANRPGGGCLFGVSLPDSVPPGAAALARSAAR

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786912, Length=264, Percent_Identity=29.9242424242424, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=29.5652173913043, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789149, Length=248, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=234, Percent_Identity=29.4871794871795, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=285, Percent_Identity=27.3684210526316, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788713, Length=244, Percent_Identity=23.7704918032787, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=27.0833333333333, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=27.3913043478261, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=27.3858921161826, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082128, Length=243, Percent_Identity=24.6913580246914, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=26.8398268398268, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788279, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=28.8990825688073, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790300, Length=269, Percent_Identity=29.368029739777, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40598; Mature: 40467

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARDRYQMLRRSFSFRPIGAYIALMFAVILVLEFSTPPEYIFGYLYVGPILLANWFLDR
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
RSTVFVTSLGVVLTLANLLFPRDALASSVAVVDRSITVAALLTVAYLGTQYRRIQEESAR
CCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKAALQAREELSRAQSDFVATLTHDLKTPLLGAQQTLQFFGRGQFGEVSPQQLGVIEMLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KSNHKLLALVDTLLAIYRNSLSGLPLAKQPSDLDELCAEQIVVLQDLAVSRQIELVYEGS
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECC
EGAHLPVDALQLGRVVANLVGNAINHTPRGGRVTVRLLRAPDEWRLLVEDTGTGIPPTDI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHEEEEECCCCCCCHHHH
RRVFERFYQAENTRHVPGTGLGLYLSRQIVEAHGGRIWAANRPGGGCLFGVSLPDSVPPG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCC
AAALARSAAR
HHHHHHHCCC
>Mature Secondary Structure 
PARDRYQMLRRSFSFRPIGAYIALMFAVILVLEFSTPPEYIFGYLYVGPILLANWFLDR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
RSTVFVTSLGVVLTLANLLFPRDALASSVAVVDRSITVAALLTVAYLGTQYRRIQEESAR
CCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKAALQAREELSRAQSDFVATLTHDLKTPLLGAQQTLQFFGRGQFGEVSPQQLGVIEMLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KSNHKLLALVDTLLAIYRNSLSGLPLAKQPSDLDELCAEQIVVLQDLAVSRQIELVYEGS
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECC
EGAHLPVDALQLGRVVANLVGNAINHTPRGGRVTVRLLRAPDEWRLLVEDTGTGIPPTDI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHEEEEECCCCCCCHHHH
RRVFERFYQAENTRHVPGTGLGLYLSRQIVEAHGGRIWAANRPGGGCLFGVSLPDSVPPG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCC
AAALARSAAR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]