Click here to switch to the map view.

The map label for this gene is napA [H]

Identifier: 37520083

GI number: 37520083

Start: 552338

End: 553597

Strand: Direct

Name: napA [H]

Synonym: glr0514

Alternate gene names: 37520083

Gene position: 552338-553597 (Clockwise)

Preceding gene: 37520082

Following gene: 37520084

Centisome position: 11.86

GC content: 63.97

Gene sequence:

>1260_bases
ATGTCTACCGGGGCAACTCAACGGAGTTATGCCATGTTCTATCCACTCATCGCGGCATCGGCTCCCGGAGCCCTGCCCGA
GGGCCTTTTGAGCTTGGGGCAGTTGCTTGCGAGCCTCATCGTCATCTATGTCGCGAGCAAACTCGGCGGCGAGCTGGCCC
TGCGTCTCAGGCAACCCGCCGTCTTGGGCGAACTGGTGGCGGGGCTGGCGGTCGGCATCTCGGGACTCAAGCTCATCGAT
CCGACCCAGCCGGTGCTGCTGCTGCTTGCCCAGGTGGGCGTCACCCTGCTGCTGTTTGAAATCGGTCTGGAATCGGATCT
GCGGGGATTGCTCAAGCTTGGTCCCCAGGCGGTGGCCGTGGCGGCGGTGGGTATGGCGGTACCCTTCGCCCTGGGTTACG
GCGTGATGAAATTTGCCGGAGCGGGGGAACTGCTCGCCATCTTCGTGGGCGCCTCCTCCACTGCCACCAGCATCGGGATC
AGCGCCAAGGTGCTCTCGGATCTGGGCTACCTCAAGCGCACCGAGGGGCAGATTATTCTCGGAGCGGCGGTGCTCGACGA
CATTTTGGGGGTGATCATACTCTCGGTGGTGGCGGGGATCGCCCAGGGGGGTAGCCTTGAGCTGGGCGAGGTGGCCCGCA
TCGTCTTCTCGTCGCTGGGCTTTTTGGTGGGAGCGATCGTGATTGGCAACCGCTTTATGCCCGTTTTTTTGGGGATCGTG
CGGCGGCTGCGCACCCGCGGCGAGTTGCTCACCGCCTCGCTGGTCTTTGCCTTCGCCCTTGCGTACCTGGCCGAACTGTT
GGGCTCCGCCGCGATCATCGGTGCCTTTGCCGCCGGATTGGTGCTCGCTGAAACCGACAAGCGCCACGACCTCGAAGCGC
AACTCAGGCCGGTCACCGACTTTTTTCTGCCGATTTTTTTTATCACCGTCGGCGCTGGGGTGAATCTGGCGCTGCTGGGC
AATCAGCAGGCGCTGTTGCTGGCCGGGGGATTGAGCGTCACTGCCGTGCTGGGCAAACTGGTGTGCGGCTGGGCGGCATT
CGGTGTCAAGGCCAACAAATTGGCGATCGGCGCCGGGATGGTGCCCCGCGGCGAAGTCGGCCTGGTCTTTGCCAGCGTCG
GCCTGGCGTCCGGGGTGCTCACCGGCGTCAACCACACCGCCGTGGTGATCATGGTGATTCTCACCACGTTTTTTGGACCG
CTGCTGTTGGGGCTGCTGTTGCGCCGCGAACCGGTGGCGCTCACCGCCGGCAGTTCTTGA

Upstream 100 bases:

>100_bases
TGTACTCACCGCGGCCACACAGTCAGCCGGTGTATCTTAAGGAGGATCAATTCTCCCGGTGTGGTGGAGTCTGTCAGACA
GGTGAGTCGTTTGTCTCACT

Downstream 100 bases:

>100_bases
AGCGGCACCACCCCAGGAGTACGCATGCCCACCCTCTCGCCCGCCCGCACCATCTGCCTGGGGTTTTTGCTGGTTATCGC
GATCGGTACCTCGCTGCTGC

Product: Na+/H+-exchanging protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSTGATQRSYAMFYPLIAASAPGALPEGLLSLGQLLASLIVIYVASKLGGELALRLRQPAVLGELVAGLAVGISGLKLID
PTQPVLLLLAQVGVTLLLFEIGLESDLRGLLKLGPQAVAVAAVGMAVPFALGYGVMKFAGAGELLAIFVGASSTATSIGI
SAKVLSDLGYLKRTEGQIILGAAVLDDILGVIILSVVAGIAQGGSLELGEVARIVFSSLGFLVGAIVIGNRFMPVFLGIV
RRLRTRGELLTASLVFAFALAYLAELLGSAAIIGAFAAGLVLAETDKRHDLEAQLRPVTDFFLPIFFITVGAGVNLALLG
NQQALLLAGGLSVTAVLGKLVCGWAAFGVKANKLAIGAGMVPRGEVGLVFASVGLASGVLTGVNHTAVVIMVILTTFFGP
LLLGLLLRREPVALTAGSS

Sequences:

>Translated_419_residues
MSTGATQRSYAMFYPLIAASAPGALPEGLLSLGQLLASLIVIYVASKLGGELALRLRQPAVLGELVAGLAVGISGLKLID
PTQPVLLLLAQVGVTLLLFEIGLESDLRGLLKLGPQAVAVAAVGMAVPFALGYGVMKFAGAGELLAIFVGASSTATSIGI
SAKVLSDLGYLKRTEGQIILGAAVLDDILGVIILSVVAGIAQGGSLELGEVARIVFSSLGFLVGAIVIGNRFMPVFLGIV
RRLRTRGELLTASLVFAFALAYLAELLGSAAIIGAFAAGLVLAETDKRHDLEAQLRPVTDFFLPIFFITVGAGVNLALLG
NQQALLLAGGLSVTAVLGKLVCGWAAFGVKANKLAIGAGMVPRGEVGLVFASVGLASGVLTGVNHTAVVIMVILTTFFGP
LLLGLLLRREPVALTAGSS
>Mature_418_residues
STGATQRSYAMFYPLIAASAPGALPEGLLSLGQLLASLIVIYVASKLGGELALRLRQPAVLGELVAGLAVGISGLKLIDP
TQPVLLLLAQVGVTLLLFEIGLESDLRGLLKLGPQAVAVAAVGMAVPFALGYGVMKFAGAGELLAIFVGASSTATSIGIS
AKVLSDLGYLKRTEGQIILGAAVLDDILGVIILSVVAGIAQGGSLELGEVARIVFSSLGFLVGAIVIGNRFMPVFLGIVR
RLRTRGELLTASLVFAFALAYLAELLGSAAIIGAFAAGLVLAETDKRHDLEAQLRPVTDFFLPIFFITVGAGVNLALLGN
QQALLLAGGLSVTAVLGKLVCGWAAFGVKANKLAIGAGMVPRGEVGLVFASVGLASGVLTGVNHTAVVIMVILTTFFGPL
LLGLLLRREPVALTAGSS

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=272, Percent_Identity=28.3088235294118, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789749, Length=369, Percent_Identity=27.6422764227642, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786685, Length=382, Percent_Identity=27.7486910994764, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322367, Length=380, Percent_Identity=26.5789473684211, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 42702; Mature: 42570

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGATQRSYAMFYPLIAASAPGALPEGLLSLGQLLASLIVIYVASKLGGELALRLRQPA
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHH
VLGELVAGLAVGISGLKLIDPTQPVLLLLAQVGVTLLLFEIGLESDLRGLLKLGPQAVAV
HHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
AAVGMAVPFALGYGVMKFAGAGELLAIFVGASSTATSIGISAKVLSDLGYLKRTEGQIIL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHCCCCCCEEE
GAAVLDDILGVIILSVVAGIAQGGSLELGEVARIVFSSLGFLVGAIVIGNRFMPVFLGIV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RRLRTRGELLTASLVFAFALAYLAELLGSAAIIGAFAAGLVLAETDKRHDLEAQLRPVTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
FFLPIFFITVGAGVNLALLGNQQALLLAGGLSVTAVLGKLVCGWAAFGVKANKLAIGAGM
HHHHHHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
VPRGEVGLVFASVGLASGVLTGVNHTAVVIMVILTTFFGPLLLGLLLRREPVALTAGSS
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
>Mature Secondary Structure 
STGATQRSYAMFYPLIAASAPGALPEGLLSLGQLLASLIVIYVASKLGGELALRLRQPA
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHH
VLGELVAGLAVGISGLKLIDPTQPVLLLLAQVGVTLLLFEIGLESDLRGLLKLGPQAVAV
HHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
AAVGMAVPFALGYGVMKFAGAGELLAIFVGASSTATSIGISAKVLSDLGYLKRTEGQIIL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHCCCCCCEEE
GAAVLDDILGVIILSVVAGIAQGGSLELGEVARIVFSSLGFLVGAIVIGNRFMPVFLGIV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RRLRTRGELLTASLVFAFALAYLAELLGSAAIIGAFAAGLVLAETDKRHDLEAQLRPVTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
FFLPIFFITVGAGVNLALLGNQQALLLAGGLSVTAVLGKLVCGWAAFGVKANKLAIGAGM
HHHHHHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
VPRGEVGLVFASVGLASGVLTGVNHTAVVIMVILTTFFGPLLLGLLLRREPVALTAGSS
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]