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The map label for this gene is ppnK

Identifier: 37520042

GI number: 37520042

Start: 505528

End: 506460

Strand: Reverse

Name: ppnK

Synonym: gll0473

Alternate gene names: 37520042

Gene position: 506460-505528 (Counterclockwise)

Preceding gene: 37520046

Following gene: 37520029

Centisome position: 10.87

GC content: 62.49

Gene sequence:

>933_bases
ATGCCTGCTTTTGAAACCGTCGTTATTGCCTACCGGGCCGAAGAAGCCCACAGCCGGATAGCCGCCGACCGGTGCGAATC
CCTTCTGCACAAAGTCGGCTGCCGGGTGCTCAAAGGGCCCACCGGTCCCCAAGACAATCCCTACCCGCACTTTCTCGAGG
CGACGGGCGGGCACATCGATCTGGCCATCGTCCTGGGGGGCGACGGCTCGATTCTGGCGGCGGCCCGCTATCTGGCTGCC
GTCGATGTGCCGATTCTGGCGGTCAACGTCGGCGGACATCTGGGCTTTCTTACCCAACCGCCGGAGGTACTGGGAGGGCG
CTACTGGGAACGGTTGCTCGCAGGCGAGTGGGAACTGGAAAAGCGCATGATGCTGCAGGCATCGCTCACCGGGCCGCCGC
CGCTGCCGGAGCGGCAGCCTTACTTTTGCCTCAACGAGTTTTGCTTGAAGCCGGCAAGTGAGATGCGTCTTACCAGCATC
ATTTTGGAACTGGCCATCGACGGTGAGATTATCGATCAGATCCATGGGGACGGATTGCTCGTCTCAACACCGACCGGTTC
CACCTCCTACACCGTAGCCGCCAACGGACCGATCATCGCGCCATCGCTGCAGGCGATTACCATCACGCCCATCTGCCCAC
TATCGCTTTCGAGCCGACCGGTGGTCCTGCCGGCCACCGGCACCATCGAGGTGAGTCCCCTGCGCGACCCCGACCTCAAC
ATCAAACTTTGGAGCGACGGCGCTTTTGCCGCCCCCGTGCACCCTTGCCAGACAGTGCGCATTGAGGTGGCCCGACACCC
AACGCGCCTGCTCATCCTGGAGGAGGATCATTCTTACTTTCGTACCCTGCGCGAGAAGCTCAAATGGGCAGGAACGCGCA
TCCAGGCTGAGCGCGACCCGGAATGCCTGCTGCCCCCCCAGAATCACCCTTGA

Upstream 100 bases:

>100_bases
GCATCGGTATGTCCTTCTAGTAGATCGACCATGTCCGGGCCGGATATGCTGTGGTGAGGGCTGCCTATCGCGGTCGGTCC
ATCCCAATCGACATATCCCC

Downstream 100 bases:

>100_bases
GTCTTACAACTCGCGTTGGCCTGCCAGTTTATAACTGTGTACGTCCGTGCAGGTGCCAAACCAGCGCAACACCTGGCCGT
CGCCGTCGTGGATGGGCATC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPAFETVVIAYRAEEAHSRIAADRCESLLHKVGCRVLKGPTGPQDNPYPHFLEATGGHIDLAIVLGGDGSILAAARYLAA
VDVPILAVNVGGHLGFLTQPPEVLGGRYWERLLAGEWELEKRMMLQASLTGPPPLPERQPYFCLNEFCLKPASEMRLTSI
ILELAIDGEIIDQIHGDGLLVSTPTGSTSYTVAANGPIIAPSLQAITITPICPLSLSSRPVVLPATGTIEVSPLRDPDLN
IKLWSDGAFAAPVHPCQTVRIEVARHPTRLLILEEDHSYFRTLREKLKWAGTRIQAERDPECLLPPQNHP

Sequences:

>Translated_310_residues
MPAFETVVIAYRAEEAHSRIAADRCESLLHKVGCRVLKGPTGPQDNPYPHFLEATGGHIDLAIVLGGDGSILAAARYLAA
VDVPILAVNVGGHLGFLTQPPEVLGGRYWERLLAGEWELEKRMMLQASLTGPPPLPERQPYFCLNEFCLKPASEMRLTSI
ILELAIDGEIIDQIHGDGLLVSTPTGSTSYTVAANGPIIAPSLQAITITPICPLSLSSRPVVLPATGTIEVSPLRDPDLN
IKLWSDGAFAAPVHPCQTVRIEVARHPTRLLILEEDHSYFRTLREKLKWAGTRIQAERDPECLLPPQNHP
>Mature_309_residues
PAFETVVIAYRAEEAHSRIAADRCESLLHKVGCRVLKGPTGPQDNPYPHFLEATGGHIDLAIVLGGDGSILAAARYLAAV
DVPILAVNVGGHLGFLTQPPEVLGGRYWERLLAGEWELEKRMMLQASLTGPPPLPERQPYFCLNEFCLKPASEMRLTSII
LELAIDGEIIDQIHGDGLLVSTPTGSTSYTVAANGPIIAPSLQAITITPICPLSLSSRPVVLPATGTIEVSPLRDPDLNI
KLWSDGAFAAPVHPCQTVRIEVARHPTRLLILEEDHSYFRTLREKLKWAGTRIQAERDPECLLPPQNHP

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=267, Percent_Identity=28.8389513108614, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788968, Length=240, Percent_Identity=37.0833333333333, Blast_Score=134, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6320794, Length=280, Percent_Identity=30, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325068, Length=193, Percent_Identity=32.6424870466321, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322509, Length=194, Percent_Identity=29.8969072164948, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28573830, Length=257, Percent_Identity=32.6848249027237, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573832, Length=258, Percent_Identity=32.5581395348837, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161077047, Length=258, Percent_Identity=32.5581395348837, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573828, Length=257, Percent_Identity=32.6848249027237, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573826, Length=258, Percent_Identity=32.5581395348837, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129957, Length=280, Percent_Identity=30, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363323, Length=280, Percent_Identity=30, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653422, Length=280, Percent_Identity=30, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363321, Length=281, Percent_Identity=29.8932384341637, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653424, Length=280, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK1_GLOVI (Q7NND8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923419.1
- ProteinModelPortal:   Q7NND8
- SMR:   Q7NND8
- GeneID:   2599674
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll0473
- NMPDR:   fig|251221.1.peg.473
- HOGENOM:   HBG713904
- OMA:   KLHWAGS
- ProtClustDB:   PRK02645
- BioCyc:   GVIO251221:GLL0473-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33780; Mature: 33649

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFETVVIAYRAEEAHSRIAADRCESLLHKVGCRVLKGPTGPQDNPYPHFLEATGGHID
CCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEECCCCEEE
LAIVLGGDGSILAAARYLAAVDVPILAVNVGGHLGFLTQPPEVLGGRYWERLLAGEWELE
EEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCHHH
KRMMLQASLTGPPPLPERQPYFCLNEFCLKPASEMRLTSIILELAIDGEIIDQIHGDGLL
HHHEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCEEE
VSTPTGSTSYTVAANGPIIAPSLQAITITPICPLSLSSRPVVLPATGTIEVSPLRDPDLN
EECCCCCCEEEEEECCCEECCCCCEEEECCCCCCCCCCCCEEEECCCEEEECCCCCCCCE
IKLWSDGAFAAPVHPCQTVRIEVARHPTRLLILEEDHSYFRTLREKLKWAGTRIQAERDP
EEEECCCCEECCCCCCEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCC
ECLLPPQNHP
CEECCCCCCC
>Mature Secondary Structure 
PAFETVVIAYRAEEAHSRIAADRCESLLHKVGCRVLKGPTGPQDNPYPHFLEATGGHID
CCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEECCCCEEE
LAIVLGGDGSILAAARYLAAVDVPILAVNVGGHLGFLTQPPEVLGGRYWERLLAGEWELE
EEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCHHH
KRMMLQASLTGPPPLPERQPYFCLNEFCLKPASEMRLTSIILELAIDGEIIDQIHGDGLL
HHHEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCEEE
VSTPTGSTSYTVAANGPIIAPSLQAITITPICPLSLSSRPVVLPATGTIEVSPLRDPDLN
EECCCCCCEEEEEECCCEECCCCCEEEECCCCCCCCCCCCEEEECCCEEEECCCCCCCCE
IKLWSDGAFAAPVHPCQTVRIEVARHPTRLLILEEDHSYFRTLREKLKWAGTRIQAERDP
EEEECCCCEECCCCCCEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCC
ECLLPPQNHP
CEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292