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The map label for this gene is rfbA [H]

Identifier: 37520038

GI number: 37520038

Start: 502369

End: 503244

Strand: Direct

Name: rfbA [H]

Synonym: gvip049

Alternate gene names: 37520038

Gene position: 502369-503244 (Clockwise)

Preceding gene: 37520037

Following gene: 37520039

Centisome position: 10.78

GC content: 63.81

Gene sequence:

>876_bases
ATGAAGGGGATCATATTGGCTGGGGGCAAGGGCACGCGGCTCTACCCCCTCACCCTGGGGGTCAGCAAACAACTGCTGCC
CGTCTACGACAAACCGATGATCTACTACCCGCTGGCCACCCTGATGCTCGCCGGCATCCGCGAGGTGCTGCTCATCTCCA
CCCCCCGCGACCTGCCCGCCTACCAAGAACTGCTCGGCGACGGCTCGCGCTACGGCATCGAGATCCGCTACTGCGTCCAG
CCGAGCCCCGACGGCCTCGCCCAGGCCTTCGTCCTCGGACGCGAGTTTTTGGAGGGCGGCCCGGCCTGTCTGATCTTGGG
CGACAACATCTTCTATGGCTACGGCCTGGGCGAAATGCTTCAAAAAGCGGCCACCCGCACGTTCGGCTCGACGATCTTTG
CCTACCGCGTCTCGGACCCAGAGCGCTACGGGGTGGTGGAGTACGACGAGCAAGGGCGGGTGCTGTCGATCGACGAGAAG
CCCAAAGCGCCGAAATCCAACTACGCGGTGGTCGGTCTGTATTTTTACGACGCACAGATAAGCGAGATCGCCGCGGGGGT
GAAGCCCTCGGCGCGGGGGGAACTGGAGATCACCGACGTCAACAACGCCTACCTGGAGCGGGGGTTGTTGCGGGTGGAGC
GTCTCGGTCGGGGGATGGCGTGGCTGGACACCGGCACGCACGAGTCGCTGTTGCAGGCGTCGAATTTTATCGAGACGATC
GAGAAGCGGCAGGGTCTGAAGGTGGCGTGTCTGGAGGAGATCGCCTACCAGATGGGTCACATCGGTGTCGAGGCGGTGCT
GGAGCAGGCGGGGCGGCTGGGCAAGACCGAGTACGGCCAGTACCTCAGAAACCTCGTCGAAAGGGGAAACCGGTGA

Upstream 100 bases:

>100_bases
TCCATTGGTATCTCTCGCAACACCGCGACTGGGTGGAGCAGGTGCTGAGCGGTGATTACAAAGAGTGGTTTGCCATCAAC
TATCGCGGGAGGACAGCCTG

Downstream 100 bases:

>100_bases
GATGCGCTTTATCGCGACTGCCCTGCCCGGTGTGGTCGTGATTGAACCGGCCGTTTACGAGCAAAGCTGCGGGTATTTTT
TTGAAAGCTATCAGCGACAA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKGIILAGGKGTRLYPLTLGVSKQLLPVYDKPMIYYPLATLMLAGIREVLLISTPRDLPAYQELLGDGSRYGIEIRYCVQ
PSPDGLAQAFVLGREFLEGGPACLILGDNIFYGYGLGEMLQKAATRTFGSTIFAYRVSDPERYGVVEYDEQGRVLSIDEK
PKAPKSNYAVVGLYFYDAQISEIAAGVKPSARGELEITDVNNAYLERGLLRVERLGRGMAWLDTGTHESLLQASNFIETI
EKRQGLKVACLEEIAYQMGHIGVEAVLEQAGRLGKTEYGQYLRNLVERGNR

Sequences:

>Translated_291_residues
MKGIILAGGKGTRLYPLTLGVSKQLLPVYDKPMIYYPLATLMLAGIREVLLISTPRDLPAYQELLGDGSRYGIEIRYCVQ
PSPDGLAQAFVLGREFLEGGPACLILGDNIFYGYGLGEMLQKAATRTFGSTIFAYRVSDPERYGVVEYDEQGRVLSIDEK
PKAPKSNYAVVGLYFYDAQISEIAAGVKPSARGELEITDVNNAYLERGLLRVERLGRGMAWLDTGTHESLLQASNFIETI
EKRQGLKVACLEEIAYQMGHIGVEAVLEQAGRLGKTEYGQYLRNLVERGNR
>Mature_291_residues
MKGIILAGGKGTRLYPLTLGVSKQLLPVYDKPMIYYPLATLMLAGIREVLLISTPRDLPAYQELLGDGSRYGIEIRYCVQ
PSPDGLAQAFVLGREFLEGGPACLILGDNIFYGYGLGEMLQKAATRTFGSTIFAYRVSDPERYGVVEYDEQGRVLSIDEK
PKAPKSNYAVVGLYFYDAQISEIAAGVKPSARGELEITDVNNAYLERGLLRVERLGRGMAWLDTGTHESLLQASNFIETI
EKRQGLKVACLEEIAYQMGHIGVEAVLEQAGRLGKTEYGQYLRNLVERGNR

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=182, Percent_Identity=29.6703296703297, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI11761619, Length=182, Percent_Identity=29.6703296703297, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788351, Length=289, Percent_Identity=63.3217993079585, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=63.986013986014, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI133931050, Length=181, Percent_Identity=30.939226519337, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320148, Length=255, Percent_Identity=29.0196078431373, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=25.4980079681275, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=25.4980079681275, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32172; Mature: 32172

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGKGTRLYPLTLGVSKQLLPVYDKPMIYYPLATLMLAGIREVLLISTPRDLPA
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHH
YQELLGDGSRYGIEIRYCVQPSPDGLAQAFVLGREFLEGGPACLILGDNIFYGYGLGEML
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEECCHHHHH
QKAATRTFGSTIFAYRVSDPERYGVVEYDEQGRVLSIDEKPKAPKSNYAVVGLYFYDAQI
HHHHHHHHCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
SEIAAGVKPSARGELEITDVNNAYLERGLLRVERLGRGMAWLDTGTHESLLQASNFIETI
HHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
EKRQGLKVACLEEIAYQMGHIGVEAVLEQAGRLGKTEYGQYLRNLVERGNR
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGIILAGGKGTRLYPLTLGVSKQLLPVYDKPMIYYPLATLMLAGIREVLLISTPRDLPA
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHH
YQELLGDGSRYGIEIRYCVQPSPDGLAQAFVLGREFLEGGPACLILGDNIFYGYGLGEML
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEECCHHHHH
QKAATRTFGSTIFAYRVSDPERYGVVEYDEQGRVLSIDEKPKAPKSNYAVVGLYFYDAQI
HHHHHHHHCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
SEIAAGVKPSARGELEITDVNNAYLERGLLRVERLGRGMAWLDTGTHESLLQASNFIETI
HHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
EKRQGLKVACLEEIAYQMGHIGVEAVLEQAGRLGKTEYGQYLRNLVERGNR
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]