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The map label for this gene is rfbB [H]

Identifier: 37520037

GI number: 37520037

Start: 501287

End: 502369

Strand: Direct

Name: rfbB [H]

Synonym: gvip048

Alternate gene names: 37520037

Gene position: 501287-502369 (Clockwise)

Preceding gene: 37520036

Following gene: 37520038

Centisome position: 10.76

GC content: 58.36

Gene sequence:

>1083_bases
ATGGACAAGCCAACGATATTGGTGACCGGCGGCGCTGGATTCATTGGCGCCAACTTCGTTTGCGCCTGGCTTTCGCGGCA
CAATGGCACCCTTGTCAACCTCGACAAGCTCACCTACGCCGGCAATCCCGCCAACCTCCATGCCCTTCAGGCCGATCCCC
GGCATGTGTTCGTCCGGGGGGACATTTGCGACCCCGAGTTGATTGCACAGTTACTGGCGCGTTTCCGTCCACGCTATATC
GTCAATTTTGCCGCCGAAAGTCACGTCGATCGCTCCATTCATTCTCCCGATGCCTTTGTGAAAACCAATGTCGACGGTGT
TTTTTTGCTGCTTGAGGCGGCTTTGCACCACTGGAAGCAATTGACCCAGCCTGAGGTGGAAGATTTCCGATTTTTGCAGG
TATCCACCGACGAAGTGTACGGCTCGCTGTCTGCGGAGGAGTCGGCATTTTCGGAGACGACGCCCTACCGGCCCAACAGC
CCCTACGCCGCTTCCAAGGCGGCTGGCGATCATTTGGTGCGCGCCTACCACCGCACCTACGGATTGCCGGTGCTCACCAC
CAACTGCTCCAACAACTACGGCCCCTACCAGCATCCCGAGAAACTCATTCCCCTGATGCTGCTTAACGCCCTGGCCGGCA
AAGCGTTGCCTGTCTATGGCGACGGCGCCAACATCCGCGACTGGTTGTTTGTGGAGGATCACTGCCGTGCCATTGAACGA
GTACTCGATTCGGGCACACCCGGCCAGACCTACAATGTCGGCGGCCACAATGAGAAGACCAATCTGGAGGTAATCCGCAC
GCTGTGCGCAATTCTCGATCAGGAGCGCCCGCGCTTGGGCAGCGGCAGTTACCGCGAACAGATCCGTTTTGTCGCCGACC
GGCCTGGTCACGATCGCCGCTACGCTATCGATGCAAGCAAGATTGGGCGCGAACTGGGTTGGAAGCCGGTTGAAAGCTTC
GAGAGCGGCATTCGCAAAACGGTCCATTGGTATCTCTCGCAACACCGCGACTGGGTGGAGCAGGTGCTGAGCGGTGATTA
CAAAGAGTGGTTTGCCATCAACTATCGCGGGAGGACAGCCTGA

Upstream 100 bases:

>100_bases
GGTTTGCCGTCACGGCAACCTTGCGGGCCAGCACCACGGCACAGTCCCCTGGCTGCGTGTTTTGTCGTCCACTGCCCACC
AAAACGTTTTGAGAGAAAAA

Downstream 100 bases:

>100_bases
TGAAGGGGATCATATTGGCTGGGGGCAAGGGCACGCGGCTCTACCCCCTCACCCTGGGGGTCAGCAAACAACTGCTGCCC
GTCTACGACAAACCGATGAT

Product: dTDP-glucose 4-6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDKPTILVTGGAGFIGANFVCAWLSRHNGTLVNLDKLTYAGNPANLHALQADPRHVFVRGDICDPELIAQLLARFRPRYI
VNFAAESHVDRSIHSPDAFVKTNVDGVFLLLEAALHHWKQLTQPEVEDFRFLQVSTDEVYGSLSAEESAFSETTPYRPNS
PYAASKAAGDHLVRAYHRTYGLPVLTTNCSNNYGPYQHPEKLIPLMLLNALAGKALPVYGDGANIRDWLFVEDHCRAIER
VLDSGTPGQTYNVGGHNEKTNLEVIRTLCAILDQERPRLGSGSYREQIRFVADRPGHDRRYAIDASKIGRELGWKPVESF
ESGIRKTVHWYLSQHRDWVEQVLSGDYKEWFAINYRGRTA

Sequences:

>Translated_360_residues
MDKPTILVTGGAGFIGANFVCAWLSRHNGTLVNLDKLTYAGNPANLHALQADPRHVFVRGDICDPELIAQLLARFRPRYI
VNFAAESHVDRSIHSPDAFVKTNVDGVFLLLEAALHHWKQLTQPEVEDFRFLQVSTDEVYGSLSAEESAFSETTPYRPNS
PYAASKAAGDHLVRAYHRTYGLPVLTTNCSNNYGPYQHPEKLIPLMLLNALAGKALPVYGDGANIRDWLFVEDHCRAIER
VLDSGTPGQTYNVGGHNEKTNLEVIRTLCAILDQERPRLGSGSYREQIRFVADRPGHDRRYAIDASKIGRELGWKPVESF
ESGIRKTVHWYLSQHRDWVEQVLSGDYKEWFAINYRGRTA
>Mature_360_residues
MDKPTILVTGGAGFIGANFVCAWLSRHNGTLVNLDKLTYAGNPANLHALQADPRHVFVRGDICDPELIAQLLARFRPRYI
VNFAAESHVDRSIHSPDAFVKTNVDGVFLLLEAALHHWKQLTQPEVEDFRFLQVSTDEVYGSLSAEESAFSETTPYRPNS
PYAASKAAGDHLVRAYHRTYGLPVLTTNCSNNYGPYQHPEKLIPLMLLNALAGKALPVYGDGANIRDWLFVEDHCRAIER
VLDSGTPGQTYNVGGHNEKTNLEVIRTLCAILDQERPRLGSGSYREQIRFVADRPGHDRRYAIDASKIGRELGWKPVESF
ESGIRKTVHWYLSQHRDWVEQVLSGDYKEWFAINYRGRTA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=35.0877192982456, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=25.0746268656716, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4504031, Length=355, Percent_Identity=23.6619718309859, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=57.3407202216066, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=56.8115942028986, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788366, Length=302, Percent_Identity=23.841059602649, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788365, Length=258, Percent_Identity=26.7441860465116, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=350, Percent_Identity=34.5714285714286, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=32.5443786982249, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17507723, Length=212, Percent_Identity=29.2452830188679, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=193, Percent_Identity=29.0155440414508, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=193, Percent_Identity=29.0155440414508, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=193, Percent_Identity=29.0155440414508, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=193, Percent_Identity=29.0155440414508, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=193, Percent_Identity=29.0155440414508, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=23.2835820895522, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=25.3731343283582, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=23.9644970414201, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40532; Mature: 40532

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKPTILVTGGAGFIGANFVCAWLSRHNGTLVNLDKLTYAGNPANLHALQADPRHVFVRG
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEHHEEECCCCCCEEEEECCCCEEEEEC
DICDPELIAQLLARFRPRYIVNFAAESHVDRSIHSPDAFVKTNVDGVFLLLEAALHHWKQ
CCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LTQPEVEDFRFLQVSTDEVYGSLSAEESAFSETTPYRPNSPYAASKAAGDHLVRAYHRTY
CCCCCCHHEEEEEEEHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GLPVLTTNCSNNYGPYQHPEKLIPLMLLNALAGKALPVYGDGANIRDWLFVEDHCRAIER
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEHHHHHHHHH
VLDSGTPGQTYNVGGHNEKTNLEVIRTLCAILDQERPRLGSGSYREQIRFVADRPGHDRR
HHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCE
YAIDASKIGRELGWKPVESFESGIRKTVHWYLSQHRDWVEQVLSGDYKEWFAINYRGRTA
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MDKPTILVTGGAGFIGANFVCAWLSRHNGTLVNLDKLTYAGNPANLHALQADPRHVFVRG
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEHHEEECCCCCCEEEEECCCCEEEEEC
DICDPELIAQLLARFRPRYIVNFAAESHVDRSIHSPDAFVKTNVDGVFLLLEAALHHWKQ
CCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LTQPEVEDFRFLQVSTDEVYGSLSAEESAFSETTPYRPNSPYAASKAAGDHLVRAYHRTY
CCCCCCHHEEEEEEEHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GLPVLTTNCSNNYGPYQHPEKLIPLMLLNALAGKALPVYGDGANIRDWLFVEDHCRAIER
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEHHHHHHHHH
VLDSGTPGQTYNVGGHNEKTNLEVIRTLCAILDQERPRLGSGSYREQIRFVADRPGHDRR
HHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCE
YAIDASKIGRELGWKPVESFESGIRKTVHWYLSQHRDWVEQVLSGDYKEWFAINYRGRTA
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]