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The map label for this gene is ycf62 [H]

Identifier: 37519970

GI number: 37519970

Start: 422163

End: 422999

Strand: Reverse

Name: ycf62 [H]

Synonym: gvip035

Alternate gene names: 37519970

Gene position: 422999-422163 (Counterclockwise)

Preceding gene: 37519971

Following gene: 37519968

Centisome position: 9.08

GC content: 64.52

Gene sequence:

>837_bases
GTGGATCTGGCTCCCGAGCGCGGCTTGCACCTGCACGTCGGTCACCTCGATCACCGCTGGAGGCCCGGCTCCGAGCGCGA
TGCCCAGAGGGTGGCGGTACTGTGCAACGTCTGGGGCGTACCTTTTCACCTGGAGATTGCCCGTACCCCACCCGCGAGCG
AAGCGGCGGCCCGCGCCTGGCGCTACTGCTGGCTTGAGCAGATCGCCCGCGGTCTGGCGCTGGAGCGGGTGGTAACCGGC
CATACGCTGAGTGACCGGGCCGAGACGGTCTTGTTCAACTTGCTGCGCGGCAGCGGCGGCGCCGGTCTGGGAAGCCTCGA
TTGGGAGCGGCCCCTGGGTGGGGGGGTACGGCTGGTCAGACCTTTATTGGGAATTACCCGCGCCGAGACCGGGGCGTATT
GTCTGGCGCACCATCTGCCGATCTGCATCGATGAGAGCAACGCCAATTTGACTTTCCGCCGCAACCGCATCCGTCTGGAA
TTGATGCCATATCTGCGCGAGCACTTCAATCCACTGGTGGAGCAAACCCTGGCCCGAACCGGCGACATTTTGCACGCAGA
ATCGGAGTTGCTCGAAAGCTTGCTCGACGAACACTGGCACAGTCTGGTGCAGGAGGGACGGCTGCACCGAAATACGCTGG
CTGCTTTGCCGCTTGCCTTGCAACGGCGGGCTGTGCGCCGTTGGCTGAGCGTGGGGCTGGGGAGGCAACCCAATTTCGAG
CACATCGCCGCTGTCCTTGGTTGCTTGAGCGCTCCCAACCGCACCCGTACATCGCCTTTGGCCCATAGTCTGCTGGTGGA
GGTGCAAGGTGAGTGGCTGGGGCTGGTCGGCGGGTAG

Upstream 100 bases:

>100_bases
GCGCCGCCTGCGCCCTGCCCTCGGCCTGCTGCCGCCGGGAAGCGGCCTGCTGGTCGCGGTCTCCGGCGGCCAGGATTCGC
TGTGTCTATTGAAGCTGCTG

Downstream 100 bases:

>100_bases
GCGCCTTTTCCCTCAAAAGTTCCGCAGATCGTCCCCGCCAGTGAGCCGCACTGCCCAGATCACCTCGCGGCCCTCCGTGG
GTTCGTGCTTTTTTTGAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MDLAPERGLHLHVGHLDHRWRPGSERDAQRVAVLCNVWGVPFHLEIARTPPASEAAARAWRYCWLEQIARGLALERVVTG
HTLSDRAETVLFNLLRGSGGAGLGSLDWERPLGGGVRLVRPLLGITRAETGAYCLAHHLPICIDESNANLTFRRNRIRLE
LMPYLREHFNPLVEQTLARTGDILHAESELLESLLDEHWHSLVQEGRLHRNTLAALPLALQRRAVRRWLSVGLGRQPNFE
HIAAVLGCLSAPNRTRTSPLAHSLLVEVQGEWLGLVGG

Sequences:

>Translated_278_residues
MDLAPERGLHLHVGHLDHRWRPGSERDAQRVAVLCNVWGVPFHLEIARTPPASEAAARAWRYCWLEQIARGLALERVVTG
HTLSDRAETVLFNLLRGSGGAGLGSLDWERPLGGGVRLVRPLLGITRAETGAYCLAHHLPICIDESNANLTFRRNRIRLE
LMPYLREHFNPLVEQTLARTGDILHAESELLESLLDEHWHSLVQEGRLHRNTLAALPLALQRRAVRRWLSVGLGRQPNFE
HIAAVLGCLSAPNRTRTSPLAHSLLVEVQGEWLGLVGG
>Mature_278_residues
MDLAPERGLHLHVGHLDHRWRPGSERDAQRVAVLCNVWGVPFHLEIARTPPASEAAARAWRYCWLEQIARGLALERVVTG
HTLSDRAETVLFNLLRGSGGAGLGSLDWERPLGGGVRLVRPLLGITRAETGAYCLAHHLPICIDESNANLTFRRNRIRLE
LMPYLREHFNPLVEQTLARTGDILHAESELLESLLDEHWHSLVQEGRLHRNTLAALPLALQRRAVRRWLSVGLGRQPNFE
HIAAVLGCLSAPNRTRTSPLAHSLLVEVQGEWLGLVGG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=227, Percent_Identity=32.5991189427313, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 31025; Mature: 31025

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAPERGLHLHVGHLDHRWRPGSERDAQRVAVLCNVWGVPFHLEIARTPPASEAAARAW
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
RYCWLEQIARGLALERVVTGHTLSDRAETVLFNLLRGSGGAGLGSLDWERPLGGGVRLVR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
PLLGITRAETGAYCLAHHLPICIDESNANLTFRRNRIRLELMPYLREHFNPLVEQTLART
HHHHHHHCCCCHHHHHHCCCEEEECCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHHHHC
GDILHAESELLESLLDEHWHSLVQEGRLHRNTLAALPLALQRRAVRRWLSVGLGRQPNFE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HIAAVLGCLSAPNRTRTSPLAHSLLVEVQGEWLGLVGG
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure
MDLAPERGLHLHVGHLDHRWRPGSERDAQRVAVLCNVWGVPFHLEIARTPPASEAAARAW
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
RYCWLEQIARGLALERVVTGHTLSDRAETVLFNLLRGSGGAGLGSLDWERPLGGGVRLVR
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
PLLGITRAETGAYCLAHHLPICIDESNANLTFRRNRIRLELMPYLREHFNPLVEQTLART
HHHHHHHCCCCHHHHHHCCCEEEECCCCCEEEEECCEEEHHHHHHHHHHHHHHHHHHHHC
GDILHAESELLESLLDEHWHSLVQEGRLHRNTLAALPLALQRRAVRRWLSVGLGRQPNFE
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HIAAVLGCLSAPNRTRTSPLAHSLLVEVQGEWLGLVGG
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]