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The map label for this gene is ispE

Identifier: 37519671

GI number: 37519671

Start: 102033

End: 102941

Strand: Reverse

Name: ispE

Synonym: gll0102

Alternate gene names: 37519671

Gene position: 102941-102033 (Counterclockwise)

Preceding gene: 37519672

Following gene: 37519670

Centisome position: 2.21

GC content: 64.25

Gene sequence:

>909_bases
ATGAAGACTGTTCGCCTGCGGGCCGCCGCCAAGATCAATCTCTACCTCGAGATACTCGGGGTACGCCCCGACAACTTCCA
CGAATTGGTGATGGTGCTGCAGAGCGTTGATCTGGCCGACACCGTCACCCTGCGCGCCGCGCCCACGACCAGGGTGAGTT
GCTCCCATCCATTGGTGCCCAACGACCGTACCAATCTGGCGGTAAGAGCGGTCGAAGTGCTTCAGAAGCACACCGGCATC
GACGAAGGAGTCGAGATTGTCATCGAGAAGCGCATCCCGGTAGCGAGCGGTCTGGCGGGCGGCTCCACCGACGCCGCCGC
AGTGCTGGCGGGGCTCAACGTGCTTTGGGATCTCGGCTTGACCCAGAGACAGCTGCAGTCTCTGGGTGCTCAAATCGGCT
CGGATATTCCCTTTTGTGTGACAGGCGGCACCGCCCTTGCTATGGGCCGGGGTGAAGTGCTCTCGCCTCTGCCGGCCCTG
AAGGGGGTCTACTTAGTGCTGGCCAAACTCGCCGATTTGCAGGTATCCACCGCCTGGGCCTACCAGACCTACCGGCGCGA
GTATCTGAGCGAGGGTACCGCGCCGCGCGCCCGCACCAGTGCTCTGCTCTCGGCGGTGGCCAGCCAGGAGGTTGCCCGCA
TCGCACCACTGTTGCACAACGACCTGGAGCGCGCCGTGCTGCCCGCCTACCCGCAGGTGAGCGCGCTGCGCGAACACTTG
GTGAGCGCCGGGGCACTGGGGGCAATGATGTCTGGCTCCGGCCCGGCGGTGTTTGGCCTTGCAAGGGACCGCGCCCACGC
CGAGGCTGTTTGTGCCGTCCTTGCGGGTGAACCCGGACTGGAACTGTTCGTCTGCAAAACCCAGCAGGCGGGCATCCTGA
TGGAAGAATCGGAGTCCTCCGACTTGTAA

Upstream 100 bases:

>100_bases
GTATGGAAGAACTCAATATCGGACACTCGATTATTGCCCGGGCGGTGCTGGTGGGTCTCGAGCGGGCGGTGCGCGAAATG
AAGCAGGCGATGATAGCTGG

Downstream 100 bases:

>100_bases
ATTTCCCACAAATAGATTTAAACTGGGGCGCGTTGCTGCCAGCCGGAGATGGACTGTATGCTCTTCACCCGCCCGCTGCG
TGGTTTTTCGTTGGTCGAGA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKTVRLRAAAKINLYLEILGVRPDNFHELVMVLQSVDLADTVTLRAAPTTRVSCSHPLVPNDRTNLAVRAVEVLQKHTGI
DEGVEIVIEKRIPVASGLAGGSTDAAAVLAGLNVLWDLGLTQRQLQSLGAQIGSDIPFCVTGGTALAMGRGEVLSPLPAL
KGVYLVLAKLADLQVSTAWAYQTYRREYLSEGTAPRARTSALLSAVASQEVARIAPLLHNDLERAVLPAYPQVSALREHL
VSAGALGAMMSGSGPAVFGLARDRAHAEAVCAVLAGEPGLELFVCKTQQAGILMEESESSDL

Sequences:

>Translated_302_residues
MKTVRLRAAAKINLYLEILGVRPDNFHELVMVLQSVDLADTVTLRAAPTTRVSCSHPLVPNDRTNLAVRAVEVLQKHTGI
DEGVEIVIEKRIPVASGLAGGSTDAAAVLAGLNVLWDLGLTQRQLQSLGAQIGSDIPFCVTGGTALAMGRGEVLSPLPAL
KGVYLVLAKLADLQVSTAWAYQTYRREYLSEGTAPRARTSALLSAVASQEVARIAPLLHNDLERAVLPAYPQVSALREHL
VSAGALGAMMSGSGPAVFGLARDRAHAEAVCAVLAGEPGLELFVCKTQQAGILMEESESSDL
>Mature_302_residues
MKTVRLRAAAKINLYLEILGVRPDNFHELVMVLQSVDLADTVTLRAAPTTRVSCSHPLVPNDRTNLAVRAVEVLQKHTGI
DEGVEIVIEKRIPVASGLAGGSTDAAAVLAGLNVLWDLGLTQRQLQSLGAQIGSDIPFCVTGGTALAMGRGEVLSPLPAL
KGVYLVLAKLADLQVSTAWAYQTYRREYLSEGTAPRARTSALLSAVASQEVARIAPLLHNDLERAVLPAYPQVSALREHL
VSAGALGAMMSGSGPAVFGLARDRAHAEAVCAVLAGEPGLELFVCKTQQAGILMEESESSDL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=167, Percent_Identity=37.7245508982036, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_GLOVI (Q7NPF3)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923048.1
- ProteinModelPortal:   Q7NPF3
- GeneID:   2600079
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll0102
- NMPDR:   fig|251221.1.peg.102
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- BioCyc:   GVIO251221:GLL0102-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31889; Mature: 31889

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVRLRAAAKINLYLEILGVRPDNFHELVMVLQSVDLADTVTLRAAPTTRVSCSHPLVP
CCCEEEEHHEEEEEEEEEEECCCCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCCCCCCC
NDRTNLAVRAVEVLQKHTGIDEGVEIVIEKRIPVASGLAGGSTDAAAVLAGLNVLWDLGL
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHCCC
TQRQLQSLGAQIGSDIPFCVTGGTALAMGRGEVLSPLPALKGVYLVLAKLADLQVSTAWA
CHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHH
YQTYRREYLSEGTAPRARTSALLSAVASQEVARIAPLLHNDLERAVLPAYPQVSALREHL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VSAGALGAMMSGSGPAVFGLARDRAHAEAVCAVLAGEPGLELFVCKTQQAGILMEESESS
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCC
DL
CC
>Mature Secondary Structure
MKTVRLRAAAKINLYLEILGVRPDNFHELVMVLQSVDLADTVTLRAAPTTRVSCSHPLVP
CCCEEEEHHEEEEEEEEEEECCCCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCCCCCCC
NDRTNLAVRAVEVLQKHTGIDEGVEIVIEKRIPVASGLAGGSTDAAAVLAGLNVLWDLGL
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHCCC
TQRQLQSLGAQIGSDIPFCVTGGTALAMGRGEVLSPLPALKGVYLVLAKLADLQVSTAWA
CHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHH
YQTYRREYLSEGTAPRARTSALLSAVASQEVARIAPLLHNDLERAVLPAYPQVSALREHL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VSAGALGAMMSGSGPAVFGLARDRAHAEAVCAVLAGEPGLELFVCKTQQAGILMEESESS
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCC
DL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292