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The map label for this gene is cmr [H]

Identifier: 34499772

GI number: 34499772

Start: 4656836

End: 4658092

Strand: Direct

Name: cmr [H]

Synonym: CV_4317

Alternate gene names: 34499772

Gene position: 4656836-4658092 (Clockwise)

Preceding gene: 34499768

Following gene: 34499773

Centisome position: 98.02

GC content: 71.2

Gene sequence:

>1257_bases
ATGCCAGAAGAATCATTCCGTCCGCTGCTGCACATTACCCGCCGCCAGGCGCTGCTGTTCTGCCTGCTGCTCACTGCCTT
CGAACTGCTGACCTACCTGGCCAGCGACATGGTGATGCCGGCCATGCTGCGCGTCACCGCCGATCTCGGCGCCGACAGCC
GCCACGTGCCCACCGCCCTCAACGCCTTCCTGCTGGGCGGCATCGCCTTCCAGTGGCTGATCGGCCCATTGTCCGACCGC
TTCGGCCGCCGGCCGCTGCTGTTATCCGGCACGCTGATCTTCGCGCTCGCCTGCCTGGCCGCCGCCGGCAGCCAGCACAT
CGCCTGGTTCAATCTGCTGCGCTTCGTCCAGGGCATCGCGCTGGGCTTCATCGTGGTGGTCAGCTACCCGGCGCTGCAGG
AAACCTTCGCCGAGCGCGACGCGGTGCGGCTGATGGCGCTGCTGGCCAATATCGCGCTCTTGTCGCCGCTGGCCGGCCCG
CTGCTGGGCAGCCTGCTGCTGGAAATCCTGTCCTGGCGCCAGCTGTTCGCCGCGCTTGCCGCGGCCACCGCGCTGGTGAT
GCTGGGACTGTGGCGCTGGATGCCGGAAACGCTGGGCGCGCCGCGGCGCGACGGCCCGGCGCAACAGCCGGTGCCGCTGC
GCCTGGGCGTGGCGCTGGCCAATTACCTAGCGCTGCTGAAGCACCGCGACTTCGTCTGCGGCAGCCTGGCGCTGGGCCTG
CTGACCCTGCCGCTGATCGCCTGGATCGGCCTGTCGCCGCTGCTGCTGATCCGCCATCTCGGCTACAGCGGCCTGGAATA
CGGCCTGTGGCAGCTGCCGGTGTTCGGCGCGGTGATCGCCGGCAACCTGCTCTTGAACCGGCTGGCCGGGCGGATGGAAT
TGTCCAGCCTGCTGCGGCTGGCGCTGTGGCCGATGGCCGGCGGCCTGCTGCTGACCGGCCTCTCCGCGCCGCTGTTCCCG
TCGCTGCCGGCGCTGGCCGCCTGCCTGGCGGTCTACGCCTTCGGCCTGGGCCTGGGCAACGCCACGCTGTACCGGCTGAC
GCTGTACAGCAGCGACAACGCCAAGGGCAGCGTCGCCGCGATGCTGGGCATGCTGTCCACCGCGATGCTGGGCGGCGGCA
GCGCCATGCTCGCCTTGCTGGATGCCGGCGGCAGCCCGGCCCGCTTCGCCGCCGGCGTCAGCCTGGCCTCGCTGCTGGCC
TTGCCGCCCTTGTGGATGCTGCTGCGCCGCCCGCGGCCCGAGCCCGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GTATTCCATCTGGTTACTTGACCTACTCTGTGCGCCCTCCGCGCCGGATCACGTCCGGCGCTCAGCCATACATAGCCATT
CTGCGAAAACTGCAACAATC

Downstream 100 bases:

>100_bases
CGCCCGTTTTCTTCATCACTGGAACCGCCATGACCACGCTTTCCCATGTCGAGGCGCTGTGCGCGCTGGAACAACCGTAC
CGCGCCGACGCCGAATGCGA

Product: multidrug translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPEESFRPLLHITRRQALLFCLLLTAFELLTYLASDMVMPAMLRVTADLGADSRHVPTALNAFLLGGIAFQWLIGPLSDR
FGRRPLLLSGTLIFALACLAAAGSQHIAWFNLLRFVQGIALGFIVVVSYPALQETFAERDAVRLMALLANIALLSPLAGP
LLGSLLLEILSWRQLFAALAAATALVMLGLWRWMPETLGAPRRDGPAQQPVPLRLGVALANYLALLKHRDFVCGSLALGL
LTLPLIAWIGLSPLLLIRHLGYSGLEYGLWQLPVFGAVIAGNLLLNRLAGRMELSSLLRLALWPMAGGLLLTGLSAPLFP
SLPALAACLAVYAFGLGLGNATLYRLTLYSSDNAKGSVAAMLGMLSTAMLGGGSAMLALLDAGGSPARFAAGVSLASLLA
LPPLWMLLRRPRPEPVAA

Sequences:

>Translated_418_residues
MPEESFRPLLHITRRQALLFCLLLTAFELLTYLASDMVMPAMLRVTADLGADSRHVPTALNAFLLGGIAFQWLIGPLSDR
FGRRPLLLSGTLIFALACLAAAGSQHIAWFNLLRFVQGIALGFIVVVSYPALQETFAERDAVRLMALLANIALLSPLAGP
LLGSLLLEILSWRQLFAALAAATALVMLGLWRWMPETLGAPRRDGPAQQPVPLRLGVALANYLALLKHRDFVCGSLALGL
LTLPLIAWIGLSPLLLIRHLGYSGLEYGLWQLPVFGAVIAGNLLLNRLAGRMELSSLLRLALWPMAGGLLLTGLSAPLFP
SLPALAACLAVYAFGLGLGNATLYRLTLYSSDNAKGSVAAMLGMLSTAMLGGGSAMLALLDAGGSPARFAAGVSLASLLA
LPPLWMLLRRPRPEPVAA
>Mature_417_residues
PEESFRPLLHITRRQALLFCLLLTAFELLTYLASDMVMPAMLRVTADLGADSRHVPTALNAFLLGGIAFQWLIGPLSDRF
GRRPLLLSGTLIFALACLAAAGSQHIAWFNLLRFVQGIALGFIVVVSYPALQETFAERDAVRLMALLANIALLSPLAGPL
LGSLLLEILSWRQLFAALAAATALVMLGLWRWMPETLGAPRRDGPAQQPVPLRLGVALANYLALLKHRDFVCGSLALGLL
TLPLIAWIGLSPLLLIRHLGYSGLEYGLWQLPVFGAVIAGNLLLNRLAGRMELSSLLRLALWPMAGGLLLTGLSAPLFPS
LPALAACLAVYAFGLGLGNATLYRLTLYSSDNAKGSVAAMLGMLSTAMLGGGSAMLALLDAGGSPARFAAGVSLASLLAL
PPLWMLLRRPRPEPVAA

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=354, Percent_Identity=46.8926553672316, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790794, Length=357, Percent_Identity=35.2941176470588, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI48994889, Length=256, Percent_Identity=32.421875, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788509, Length=298, Percent_Identity=27.8523489932886, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790146, Length=233, Percent_Identity=27.8969957081545, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082312, Length=204, Percent_Identity=32.843137254902, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=160, Percent_Identity=33.125, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44508; Mature: 44377

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEESFRPLLHITRRQALLFCLLLTAFELLTYLASDMVMPAMLRVTADLGADSRHVPTAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
NAFLLGGIAFQWLIGPLSDRFGRRPLLLSGTLIFALACLAAAGSQHIAWFNLLRFVQGIA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LGFIVVVSYPALQETFAERDAVRLMALLANIALLSPLAGPLLGSLLLEILSWRQLFAALA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AATALVMLGLWRWMPETLGAPRRDGPAQQPVPLRLGVALANYLALLKHRDFVCGSLALGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LTLPLIAWIGLSPLLLIRHLGYSGLEYGLWQLPVFGAVIAGNLLLNRLAGRMELSSLLRL
HHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALWPMAGGLLLTGLSAPLFPSLPALAACLAVYAFGLGLGNATLYRLTLYSSDNAKGSVAA
HHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHH
MLGMLSTAMLGGGSAMLALLDAGGSPARFAAGVSLASLLALPPLWMLLRRPRPEPVAA
HHHHHHHHHHCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PEESFRPLLHITRRQALLFCLLLTAFELLTYLASDMVMPAMLRVTADLGADSRHVPTAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
NAFLLGGIAFQWLIGPLSDRFGRRPLLLSGTLIFALACLAAAGSQHIAWFNLLRFVQGIA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LGFIVVVSYPALQETFAERDAVRLMALLANIALLSPLAGPLLGSLLLEILSWRQLFAALA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AATALVMLGLWRWMPETLGAPRRDGPAQQPVPLRLGVALANYLALLKHRDFVCGSLALGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LTLPLIAWIGLSPLLLIRHLGYSGLEYGLWQLPVFGAVIAGNLLLNRLAGRMELSSLLRL
HHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALWPMAGGLLLTGLSAPLFPSLPALAACLAVYAFGLGLGNATLYRLTLYSSDNAKGSVAA
HHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHH
MLGMLSTAMLGGGSAMLALLDAGGSPARFAAGVSLASLLALPPLWMLLRRPRPEPVAA
HHHHHHHHHHCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]