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The map label for this gene is ltaA [H]

Identifier: 34499764

GI number: 34499764

Start: 4651327

End: 4652355

Strand: Reverse

Name: ltaA [H]

Synonym: CV_4309

Alternate gene names: 34499764

Gene position: 4652355-4651327 (Counterclockwise)

Preceding gene: 34499765

Following gene: 34499763

Centisome position: 97.92

GC content: 70.36

Gene sequence:

>1029_bases
ATGCAGTGGATAGACCTTCGCAGCGATACCGTCACCCATCCGACCCCGGCGATGCGCCGCGCGATGGCCGAGGCCGAAGT
CGGCGACGATGTCTACGGCGACGACCCCACCGTGCTGAAGCTGGAGGCGCTGGCCGCCGACAGGCTGGGCAAGGAGGCGG
CGCTGCTCGTCCCCAGCGGCACCTTCGGCAACCAGCTGGCGCTGTTCACCCACTGCCGCCGCGGCGACGAGGTGATTGTC
GAGGACAACAGCCACATCGTCTGGCACGAGGCCGGCGCGGCGGCGGTGATCGCCGGCGCCCACCTGCGGACGATAGAGGG
CGATAACGGCCTGATGGCGGTGGACGCGATCGAGCGGCGCATCCGCGTCGGCGACGACATCCACGAGCCTCGCACCGGTC
TGATCTGCCTGGAAAACGCCCATGGCAACGGCCGCGTGCTGCCGTTGTCGGCGATGGCCGACACCGCGGCGCTGGCGCGC
GAGCACGGCGTGCCGGTGCACCTGGACGGCGCGCGGGTGTTCAACGCCGCCGCCCATCTGGGCTGCGACGTGCGCGAGGT
CACCCGCCACGTCGACAGCGTGATGGCCTGCCTGTCCAAGGGCCTGGCCGCGCCGATCGGCTCCATCCTGGCCGGTTCCG
CCGATTTCATCGATCAGGCGCGCCGCAAGCGCAAGCTGCTGGGCGGCGGCCTGCGCCAGGCCGGCGTGATCGCCGCGCCC
GGCATCCTGGCGCTGACCGAGATGGCCGCGCGTCTGGACGAGGACCACCGCAATGCCCGCTACCTGGCCGAGGGCCTGGC
CAAGCTGCCGTGCGTGGACTTGAATCCGGCCGATGTCCACATCAACCTGGTGTGGTTCCGCTTCAACGCCGACATCGACG
CCGCTGAACTGATGGCCGCGCTGAAGCAGGCCGGCTTCCTCGCCAATCCGCCGCATCAGGGCGCGATGCGCCTGGTGACC
CACTGGCAGGTAGGCCGCGCCGACATCGACCGCCTGTTGGAGGTGATGCAGCGCGTGCTCTGCGACTGA

Upstream 100 bases:

>100_bases
CGCCGATCCGCAGGCCGAGGCGCTGGAAGACGCGATTACCGACGCGCTGGCGGGTTATCTGGCCCTGCCCGCGCGGCAAC
ACACTTGAGGAGAAGCGCAG

Downstream 100 bases:

>100_bases
ACGGGCTATAACCATGAGGTAGAAACCGATGGGAGCTGGCCGCATGCGGCAACGCTTGAAGTTCGGCGCGCTGCTGGCGG
CCGCTTGCTTTTACCCGGCG

Product: L-allo-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MQWIDLRSDTVTHPTPAMRRAMAEAEVGDDVYGDDPTVLKLEALAADRLGKEAALLVPSGTFGNQLALFTHCRRGDEVIV
EDNSHIVWHEAGAAAVIAGAHLRTIEGDNGLMAVDAIERRIRVGDDIHEPRTGLICLENAHGNGRVLPLSAMADTAALAR
EHGVPVHLDGARVFNAAAHLGCDVREVTRHVDSVMACLSKGLAAPIGSILAGSADFIDQARRKRKLLGGGLRQAGVIAAP
GILALTEMAARLDEDHRNARYLAEGLAKLPCVDLNPADVHINLVWFRFNADIDAAELMAALKQAGFLANPPHQGAMRLVT
HWQVGRADIDRLLEVMQRVLCD

Sequences:

>Translated_342_residues
MQWIDLRSDTVTHPTPAMRRAMAEAEVGDDVYGDDPTVLKLEALAADRLGKEAALLVPSGTFGNQLALFTHCRRGDEVIV
EDNSHIVWHEAGAAAVIAGAHLRTIEGDNGLMAVDAIERRIRVGDDIHEPRTGLICLENAHGNGRVLPLSAMADTAALAR
EHGVPVHLDGARVFNAAAHLGCDVREVTRHVDSVMACLSKGLAAPIGSILAGSADFIDQARRKRKLLGGGLRQAGVIAAP
GILALTEMAARLDEDHRNARYLAEGLAKLPCVDLNPADVHINLVWFRFNADIDAAELMAALKQAGFLANPPHQGAMRLVT
HWQVGRADIDRLLEVMQRVLCD
>Mature_342_residues
MQWIDLRSDTVTHPTPAMRRAMAEAEVGDDVYGDDPTVLKLEALAADRLGKEAALLVPSGTFGNQLALFTHCRRGDEVIV
EDNSHIVWHEAGAAAVIAGAHLRTIEGDNGLMAVDAIERRIRVGDDIHEPRTGLICLENAHGNGRVLPLSAMADTAALAR
EHGVPVHLDGARVFNAAAHLGCDVREVTRHVDSVMACLSKGLAAPIGSILAGSADFIDQARRKRKLLGGGLRQAGVIAAP
GILALTEMAARLDEDHRNARYLAEGLAKLPCVDLNPADVHINLVWFRFNADIDAAELMAALKQAGFLANPPHQGAMRLVT
HWQVGRADIDRLLEVMQRVLCD

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=327, Percent_Identity=45.2599388379205, Blast_Score=258, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI193206522, Length=350, Percent_Identity=41.1428571428571, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI193206524, Length=350, Percent_Identity=41.1428571428571, Blast_Score=237, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI193206520, Length=350, Percent_Identity=41.1428571428571, Blast_Score=237, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6320789, Length=335, Percent_Identity=36.1194029850746, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI21355439, Length=368, Percent_Identity=42.3913043478261, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36813; Mature: 36813

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWIDLRSDTVTHPTPAMRRAMAEAEVGDDVYGDDPTVLKLEALAADRLGKEAALLVPSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEEEECC
TFGNQLALFTHCRRGDEVIVEDNSHIVWHEAGAAAVIAGAHLRTIEGDNGLMAVDAIERR
CCCCCCHHHHHCCCCCEEEEECCCEEEEECCCCEEEEECCEEEEEECCCCEEEHHHHHHH
IRVGDDIHEPRTGLICLENAHGNGRVLPLSAMADTAALAREHGVPVHLDGARVFNAAAHL
HHCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCEEECCHHHHHHHHHC
GCDVREVTRHVDSVMACLSKGLAAPIGSILAGSADFIDQARRKRKLLGGGLRQAGVIAAP
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEECC
GILALTEMAARLDEDHRNARYLAEGLAKLPCVDLNPADVHINLVWFRFNADIDAAELMAA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHH
LKQAGFLANPPHQGAMRLVTHWQVGRADIDRLLEVMQRVLCD
HHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQWIDLRSDTVTHPTPAMRRAMAEAEVGDDVYGDDPTVLKLEALAADRLGKEAALLVPSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEEEECC
TFGNQLALFTHCRRGDEVIVEDNSHIVWHEAGAAAVIAGAHLRTIEGDNGLMAVDAIERR
CCCCCCHHHHHCCCCCEEEEECCCEEEEECCCCEEEEECCEEEEEECCCCEEEHHHHHHH
IRVGDDIHEPRTGLICLENAHGNGRVLPLSAMADTAALAREHGVPVHLDGARVFNAAAHL
HHCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCEEECCHHHHHHHHHC
GCDVREVTRHVDSVMACLSKGLAAPIGSILAGSADFIDQARRKRKLLGGGLRQAGVIAAP
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEECC
GILALTEMAARLDEDHRNARYLAEGLAKLPCVDLNPADVHINLVWFRFNADIDAAELMAA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHH
LKQAGFLANPPHQGAMRLVTHWQVGRADIDRLLEVMQRVLCD
HHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]