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The map label for this gene is pgaB [H]

Identifier: 34499732

GI number: 34499732

Start: 4610850

End: 4612763

Strand: Reverse

Name: pgaB [H]

Synonym: CV_4277

Alternate gene names: 34499732

Gene position: 4612763-4610850 (Counterclockwise)

Preceding gene: 34499733

Following gene: 34499731

Centisome position: 97.09

GC content: 71.26

Gene sequence:

>1914_bases
ATGGGACGGATCGCGCTGTGGCTGTTGCTGGGATGCCTGCTGGCGCCGCTGGCGGCGGCATCCAAGCTGCTGGTGCTGGC
CTACCATCAGGTCGGAGACGGCGACGACTACGCCGTCAGCGCCGAGCAGCTGGACGGCCAGCTGGGCTGGCTGAGCCGCA
ACGGCTACCGCTTCGTCGGCGTCGACCAGATTCTGGCCGCCCGTTCCGGCGGCAAGCCGCTGCCGGCGCATCCGGTGCTG
CTGACTTTCGATGACGGCCACCGCAGCGTCTACACCCGGGCGCTGCCGCTGTTGCGGCGCTACCGCGCGCCGGCGCTGGT
CGGGCTGGTGGGCAGCTGGCTGGAGGCGTCGGCCGACGAGGCCATCGACTACGGCGATGGTCAGGCGCCGCGCGGCGACT
TCCTGTCCTGGGACGAGATCCGCGCGCTGCGGCGCAGCGGCTGGGTGGAGGTGGCCAGCCACAGCTATGCGATGCACCAT
GGCGTCGTCGCCAATCCACAGGGCAACGCCTTGCCGGCCTATACCTCGCCGGCCTATGCCGCGGCAGGCGGCTACGAAAG
CCGCGAACGCTATCTGCGGCGCGCCGCGGATGATCTGGCCCGCAACAGCGCGCTGCTGCGCGACAAGCTGGGCCGGGCGC
CCAGGGTGATGATCTGGCCTTATGGCAGCCACAGCGGCGAGCTGGACCGCCTGGCCGCATCCGCCGGCATGCCGCTCACG
CTGTCGCTGGAGGACGGCCTCAATCCGCCCGGCCAGCCGCTGTCCTCGCTGCGACGGGTGCTGGTGACCGCCGGCATGGG
CGTGGACGGATTCGCCGCCGCCATCCGCCGCCGCGAGGCGTGGCCGGATGGCCGCCAGCCGGGGCCGAGGCGCATCATGC
ACGTCGATCTCGACTATGTGTACGATCCCGATCCGGCGACGCAGGAGGCCAATCTGGGCAAGCTGCTGGAGCGGGTGGCG
CAGATGGGGCCGGACGCGGTTTATCTGCAGGCTTTCGCCGATCCCGGCGGCGACGGCGCGGTCCGCGCGCTGTATTTTCC
CAACCGCCGCCTGCCGGTGCGCGCCGACCTGTTCAATCGGGCCGCCTGGCAGCTGAGAACCCGCTGCCGGGTCAAAGTGT
ACGCCTGGATGCCGCTGCTGGCCTTCGCCGGGCCGGCGTCGCTGCCGCGGGTGGCGGCGCTGGCCGCGCCGGGGCGGCGG
GCGGCGGCGGGATATCCCCGGCTCAGCCCGTTCTCGCCGGAAGCGAAGGCGTTTATCGCGGATATTTACACCGATCTGGC
CCGCAGCGCGCGTTTCGACGGCCTGCTGTTCCACGACGACGCCACGCTGTCCGACTACGAGGACGCAAGCCCCGCGGCCC
GCGCCGCCTACCGCGCGGCCGGCCTGCCGGACGATGTGGCCGCGCTGCGCGCCGATCCGGCGCTGCAGGCGCGCTGGACC
CGCTTCAAGACCGAAGCGCTGGACGCGTTCAGCGGCCAGTTGGCCGACATTGTCCGCGACTACCAGCCGGGGCTCTATAC
TTCCCGCAACCTGTACGCGCCGGTGGTGCTGCAGCCGGCGTCCCAGGCCTGGTTCGCCCAGTCGTTTCCCGAGGCGCTGC
GCCGCTACGACGCGGTGGCGGTGATGGCGATGCCGTACATGGAAGGCGCGGCCGATCCGGCTGAGTGGATGCGGCGGCTT
TTCGACCGGGCGGCGGCGGTGCCGGGGGCGCTGGACAAGACGGTGTTCGAGCTGCAGAGCCAGGATTGGCGCAGCGGCAA
GCCGGTGGACTCGGCCGAGCTGGCATCCTCCATGCGCGCGCTGCGGCAGTGGGGCGCGCGGGCGCTGGCTTATTACCCGG
ACGATTTCTTCAACGACCGTCCGGAATTGAAGCGGGTGAGGCCCGACTTTCATCTGCCGCTCGACGCGCATTGA

Upstream 100 bases:

>100_bases
TCCGCCGCGTTATGATGAGGGTTCTGGGCGCCTGCCCACGATTTTCCCGGCCGCGGCGGTTTGCTGCCGGCAAGCGCGCA
GGCCCTGCTAGGGGGATGTC

Downstream 100 bases:

>100_bases
GCGCCGGCACTTGAATATGGCTGTCGCCACCCCCACCATTAGCGAATTCAACGTCCGTTCCAAGGGGATACCATGCCACC
CGTTCCGCACCTGGCCACCG

Product: hypothetical protein

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MGRIALWLLLGCLLAPLAAASKLLVLAYHQVGDGDDYAVSAEQLDGQLGWLSRNGYRFVGVDQILAARSGGKPLPAHPVL
LTFDDGHRSVYTRALPLLRRYRAPALVGLVGSWLEASADEAIDYGDGQAPRGDFLSWDEIRALRRSGWVEVASHSYAMHH
GVVANPQGNALPAYTSPAYAAAGGYESRERYLRRAADDLARNSALLRDKLGRAPRVMIWPYGSHSGELDRLAASAGMPLT
LSLEDGLNPPGQPLSSLRRVLVTAGMGVDGFAAAIRRREAWPDGRQPGPRRIMHVDLDYVYDPDPATQEANLGKLLERVA
QMGPDAVYLQAFADPGGDGAVRALYFPNRRLPVRADLFNRAAWQLRTRCRVKVYAWMPLLAFAGPASLPRVAALAAPGRR
AAAGYPRLSPFSPEAKAFIADIYTDLARSARFDGLLFHDDATLSDYEDASPAARAAYRAAGLPDDVAALRADPALQARWT
RFKTEALDAFSGQLADIVRDYQPGLYTSRNLYAPVVLQPASQAWFAQSFPEALRRYDAVAVMAMPYMEGAADPAEWMRRL
FDRAAAVPGALDKTVFELQSQDWRSGKPVDSAELASSMRALRQWGARALAYYPDDFFNDRPELKRVRPDFHLPLDAH

Sequences:

>Translated_637_residues
MGRIALWLLLGCLLAPLAAASKLLVLAYHQVGDGDDYAVSAEQLDGQLGWLSRNGYRFVGVDQILAARSGGKPLPAHPVL
LTFDDGHRSVYTRALPLLRRYRAPALVGLVGSWLEASADEAIDYGDGQAPRGDFLSWDEIRALRRSGWVEVASHSYAMHH
GVVANPQGNALPAYTSPAYAAAGGYESRERYLRRAADDLARNSALLRDKLGRAPRVMIWPYGSHSGELDRLAASAGMPLT
LSLEDGLNPPGQPLSSLRRVLVTAGMGVDGFAAAIRRREAWPDGRQPGPRRIMHVDLDYVYDPDPATQEANLGKLLERVA
QMGPDAVYLQAFADPGGDGAVRALYFPNRRLPVRADLFNRAAWQLRTRCRVKVYAWMPLLAFAGPASLPRVAALAAPGRR
AAAGYPRLSPFSPEAKAFIADIYTDLARSARFDGLLFHDDATLSDYEDASPAARAAYRAAGLPDDVAALRADPALQARWT
RFKTEALDAFSGQLADIVRDYQPGLYTSRNLYAPVVLQPASQAWFAQSFPEALRRYDAVAVMAMPYMEGAADPAEWMRRL
FDRAAAVPGALDKTVFELQSQDWRSGKPVDSAELASSMRALRQWGARALAYYPDDFFNDRPELKRVRPDFHLPLDAH
>Mature_636_residues
GRIALWLLLGCLLAPLAAASKLLVLAYHQVGDGDDYAVSAEQLDGQLGWLSRNGYRFVGVDQILAARSGGKPLPAHPVLL
TFDDGHRSVYTRALPLLRRYRAPALVGLVGSWLEASADEAIDYGDGQAPRGDFLSWDEIRALRRSGWVEVASHSYAMHHG
VVANPQGNALPAYTSPAYAAAGGYESRERYLRRAADDLARNSALLRDKLGRAPRVMIWPYGSHSGELDRLAASAGMPLTL
SLEDGLNPPGQPLSSLRRVLVTAGMGVDGFAAAIRRREAWPDGRQPGPRRIMHVDLDYVYDPDPATQEANLGKLLERVAQ
MGPDAVYLQAFADPGGDGAVRALYFPNRRLPVRADLFNRAAWQLRTRCRVKVYAWMPLLAFAGPASLPRVAALAAPGRRA
AAGYPRLSPFSPEAKAFIADIYTDLARSARFDGLLFHDDATLSDYEDASPAARAAYRAAGLPDDVAALRADPALQARWTR
FKTEALDAFSGQLADIVRDYQPGLYTSRNLYAPVVLQPASQAWFAQSFPEALRRYDAVAVMAMPYMEGAADPAEWMRRLF
DRAAAVPGALDKTVFELQSQDWRSGKPVDSAELASSMRALRQWGARALAYYPDDFFNDRPELKRVRPDFHLPLDAH

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=622, Percent_Identity=40.3536977491961, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 69896; Mature: 69765

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRIALWLLLGCLLAPLAAASKLLVLAYHQVGDGDDYAVSAEQLDGQLGWLSRNGYRFVG
CCHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCCCCCCEEEHHHHCCCEEEEECCCEEEEC
VDQILAARSGGKPLPAHPVLLTFDDGHRSVYTRALPLLRRYRAPALVGLVGSWLEASADE
HHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHH
AIDYGDGQAPRGDFLSWDEIRALRRSGWVEVASHSYAMHHGVVANPQGNALPAYTSPAYA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHCCEEECCCCCCCCCCCCCCHH
AAGGYESRERYLRRAADDLARNSALLRDKLGRAPRVMIWPYGSHSGELDRLAASAGMPLT
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEE
LSLEDGLNPPGQPLSSLRRVLVTAGMGVDGFAAAIRRREAWPDGRQPGPRRIMHVDLDYV
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEE
YDPDPATQEANLGKLLERVAQMGPDAVYLQAFADPGGDGAVRALYFPNRRLPVRADLFNR
ECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHH
AAWQLRTRCRVKVYAWMPLLAFAGPASLPRVAALAAPGRRAAAGYPRLSPFSPEAKAFIA
HHHHHHHHHEEEEEEHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
DIYTDLARSARFDGLLFHDDATLSDYEDASPAARAAYRAAGLPDDVAALRADPALQARWT
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
RFKTEALDAFSGQLADIVRDYQPGLYTSRNLYAPVVLQPASQAWFAQSFPEALRRYDAVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
VMAMPYMEGAADPAEWMRRLFDRAAAVPGALDKTVFELQSQDWRSGKPVDSAELASSMRA
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LRQWGARALAYYPDDFFNDRPELKRVRPDFHLPLDAH
HHHHCCCEEEECCHHHCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GRIALWLLLGCLLAPLAAASKLLVLAYHQVGDGDDYAVSAEQLDGQLGWLSRNGYRFVG
CHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCCCCCCEEEHHHHCCCEEEEECCCEEEEC
VDQILAARSGGKPLPAHPVLLTFDDGHRSVYTRALPLLRRYRAPALVGLVGSWLEASADE
HHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHH
AIDYGDGQAPRGDFLSWDEIRALRRSGWVEVASHSYAMHHGVVANPQGNALPAYTSPAYA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHCCEEECCCCCCCCCCCCCCHH
AAGGYESRERYLRRAADDLARNSALLRDKLGRAPRVMIWPYGSHSGELDRLAASAGMPLT
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEE
LSLEDGLNPPGQPLSSLRRVLVTAGMGVDGFAAAIRRREAWPDGRQPGPRRIMHVDLDYV
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEE
YDPDPATQEANLGKLLERVAQMGPDAVYLQAFADPGGDGAVRALYFPNRRLPVRADLFNR
ECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHH
AAWQLRTRCRVKVYAWMPLLAFAGPASLPRVAALAAPGRRAAAGYPRLSPFSPEAKAFIA
HHHHHHHHHEEEEEEHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
DIYTDLARSARFDGLLFHDDATLSDYEDASPAARAAYRAAGLPDDVAALRADPALQARWT
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
RFKTEALDAFSGQLADIVRDYQPGLYTSRNLYAPVVLQPASQAWFAQSFPEALRRYDAVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
VMAMPYMEGAADPAEWMRRLFDRAAAVPGALDKTVFELQSQDWRSGKPVDSAELASSMRA
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LRQWGARALAYYPDDFFNDRPELKRVRPDFHLPLDAH
HHHHCCCEEEECCHHHCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]