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The map label for this gene is IMP

Identifier: 34499684

GI number: 34499684

Start: 4562441

End: 4564702

Strand: Direct

Name: IMP

Synonym: CV_4229

Alternate gene names: 34499684

Gene position: 4562441-4564702 (Clockwise)

Preceding gene: 34499679

Following gene: 34499685

Centisome position: 96.03

GC content: 62.64

Gene sequence:

>2262_bases
ATGCACAGACAAAAACTGAATCCGCTGGCGCTGGCCCTGGCCGCCGCCTTTGCCCTGAATGCCCCCGCGGCGCTCGCCGA
TGACGAAGAGCCGCCGATAGCCGTCCCCGCCCCGCCCAAGGAGCCAGGCCAGACCGTGGTCCACGCCGACGACATGGACG
GCGAGATGAGCGTGATCCTGCACGCCAAGGGCAATGTGGTGGCGACCCGCGACGACCAGAGGGTGGAATCCGACTGGCTG
GACTATTACCAGACCAAGAACCGGGTCAAGGCCGGCGACCGCTTCCGCATGACCCGCGGCGGCGACGTCATCACCGGCAC
CACGCTGGACTACAACGTGGACACCTACAGCGGCACCGGCATGGACCCGGTGTTCTCGATGGCCAGGCAGAATCAGACCG
CGGTCAAGCCGGCGCCCGGCGCGCCGCCCGCCAAACCGGTGACCCTGCGCGGCGACGGCAGCCAGGTGGATTTCCAGGGA
CAAAACCAATATCGCGTCTACGGCAGCCGGATGACCACCTGCGATCCCGGCGACGAAGCCTGGTATCTGAAGTCGTCGCG
CACCGACCTCGATTACAACACCGGCGTCGGCGTCGCCCACAACGCCTGGATGCAGTTCTACGGCGTGCCCATCCTGTATT
CGCCATGGCTGGACTTTCCGCTCAACAGCAATCGCAAGTCCGGCTTCCTGATGCCGACCTTCAAGACCGGCAGCTCGGGC
ACCGAATTCTCGCTGCCCTACTACTGGAACATCGCGCCCAACTACGACGCCACCATCACGCCGCACATCAACGTCAAGCA
CGGCAACATGCTGGCCGGCGAATTCCGCTACCTGCAGCCCGACTACAGCGGCCGCATCTACACCGAGCAGTTGCCGAAAG
ACAAGCTGACCGGCGAGTCCCGCTACGCCTGGAGCGCCAGCCATAGCCAGAACTTCGGCCATGGCCTGAGCTTCGGCATG
GATTTCAACCAGGTATCGGACAACAACTACTTCACCGATTTCGGCGATCAGGTGGCCATCGCATCCAACGTCAACCTGAA
CCGGGAAGCCTGGCTGAACTATGCCTTGGGTTGGCAGGGCGGCGGCGGCAACGTCACCCTGCGTGCCCAGCGCTACCAGA
ACCTGACCATCAATCCGGTCCCCGGCGACATCCCCTACGCCAAGATGCCGCAGCTGACCTTCAACGCGAATCAAAGCCTG
CCGTCCGGTTTCTCGGCCAATCTGATCTCGGATCTGACCCGCTTCGACCATCCCTCGCTGCAGAATGCGGAGCGGCTGGT
GCTGTACCCCAGCGTCAGCTGGAACTTCGACCGTAGCTGGGGCTTCCTGCGGCCGAAATTGGGGGTCAACTACACCCACT
ACAACCTGGACGCCTTCCAAGGCAGTCCAAGCCATGTCCAGACCCGCACGCTGCCGATCTTCAGCACCGACGCCGGCCTG
TATTTCGACCGCGACACCCAGTTCCTGGGACGAGACCACCTGATGACGCTGGAGCCCAGGCTGTTCTACGTCAACATTCC
CAACAACAGGGATCAGAACTCGCTGCCGATGTTCGACACATCGGTCAACGACATCAATTTCGCGCAGCTGTTCACCGAAA
ACCGCTACTCCGGCTACGACCGCATCAACGGCGCCAACCAGATCACCACCGCGCTGACCAGCCGCTTCATCGACCAGAGC
AATGGTCTGGAACGACTGCGTTTGGCGGTGGGCAAGCGTTTTTATCTGAAAGACGATGTCACGCAGACCGTCAACCAGCC
CAGTTCCGACCTGCTGCTGTCCGCCGGCGGCGACCTGACCCGCGAATGGCGCTTCGACAGCAGCTATCAGTACAACCAGC
AACTGGGCATGACCGAGCGCTACAACGCCCAGTTGCGCTACAACCCGGCGGCCGGCAAGATCGCCAGCGTGCGCTACCGC
TTCGGCCGCTACGAACAGCTGGACAACTCCAACAACTACGGTCCGATGCGCCAGGTGGACGTGGCCGCGCAATGGCCGAT
CGCCCGCCGCTGGTACGCGATCGGCCGCTACAACTACTCCTTCATCGAGCGCAAGCCTATTGAGCGGCTGGCCGGCTTCG
AGTACAACGATGGCTGCTGGTCGTTGCGGATGTATAGCCAACGCTACGTCACCGACCCGACCACCACCAAGAACGCGTGG
TTCTTCCAGCTGGAACTCAAGGGCCTCGGCGCCCTGGGCAACAACGGCGTGCAGGACACGCTGCGTCTGGCCGTACCCGG
CTACACCAAGATTAACGAGTAA

Upstream 100 bases:

>100_bases
TTTCTTGAGCTGTTCCTGTCTTTGCATGTCGCTTTATCGTGGTGCTGATAGAATTGGACGATTTTATACCGCCCACTGCC
CCCAAGATATGGCTTTGTGC

Downstream 100 bases:

>100_bases
CAAAGCCTGACCATGAAAAAGACCCTGCTTGCCCTGTTGATCGCCAGCGTGATGCAAAGCGCCCTGGCGGCCCCCGCCAC
CCCGGTACGCGAAGTGGATC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MHRQKLNPLALALAAAFALNAPAALADDEEPPIAVPAPPKEPGQTVVHADDMDGEMSVILHAKGNVVATRDDQRVESDWL
DYYQTKNRVKAGDRFRMTRGGDVITGTTLDYNVDTYSGTGMDPVFSMARQNQTAVKPAPGAPPAKPVTLRGDGSQVDFQG
QNQYRVYGSRMTTCDPGDEAWYLKSSRTDLDYNTGVGVAHNAWMQFYGVPILYSPWLDFPLNSNRKSGFLMPTFKTGSSG
TEFSLPYYWNIAPNYDATITPHINVKHGNMLAGEFRYLQPDYSGRIYTEQLPKDKLTGESRYAWSASHSQNFGHGLSFGM
DFNQVSDNNYFTDFGDQVAIASNVNLNREAWLNYALGWQGGGGNVTLRAQRYQNLTINPVPGDIPYAKMPQLTFNANQSL
PSGFSANLISDLTRFDHPSLQNAERLVLYPSVSWNFDRSWGFLRPKLGVNYTHYNLDAFQGSPSHVQTRTLPIFSTDAGL
YFDRDTQFLGRDHLMTLEPRLFYVNIPNNRDQNSLPMFDTSVNDINFAQLFTENRYSGYDRINGANQITTALTSRFIDQS
NGLERLRLAVGKRFYLKDDVTQTVNQPSSDLLLSAGGDLTREWRFDSSYQYNQQLGMTERYNAQLRYNPAAGKIASVRYR
FGRYEQLDNSNNYGPMRQVDVAAQWPIARRWYAIGRYNYSFIERKPIERLAGFEYNDGCWSLRMYSQRYVTDPTTTKNAW
FFQLELKGLGALGNNGVQDTLRLAVPGYTKINE

Sequences:

>Translated_753_residues
MHRQKLNPLALALAAAFALNAPAALADDEEPPIAVPAPPKEPGQTVVHADDMDGEMSVILHAKGNVVATRDDQRVESDWL
DYYQTKNRVKAGDRFRMTRGGDVITGTTLDYNVDTYSGTGMDPVFSMARQNQTAVKPAPGAPPAKPVTLRGDGSQVDFQG
QNQYRVYGSRMTTCDPGDEAWYLKSSRTDLDYNTGVGVAHNAWMQFYGVPILYSPWLDFPLNSNRKSGFLMPTFKTGSSG
TEFSLPYYWNIAPNYDATITPHINVKHGNMLAGEFRYLQPDYSGRIYTEQLPKDKLTGESRYAWSASHSQNFGHGLSFGM
DFNQVSDNNYFTDFGDQVAIASNVNLNREAWLNYALGWQGGGGNVTLRAQRYQNLTINPVPGDIPYAKMPQLTFNANQSL
PSGFSANLISDLTRFDHPSLQNAERLVLYPSVSWNFDRSWGFLRPKLGVNYTHYNLDAFQGSPSHVQTRTLPIFSTDAGL
YFDRDTQFLGRDHLMTLEPRLFYVNIPNNRDQNSLPMFDTSVNDINFAQLFTENRYSGYDRINGANQITTALTSRFIDQS
NGLERLRLAVGKRFYLKDDVTQTVNQPSSDLLLSAGGDLTREWRFDSSYQYNQQLGMTERYNAQLRYNPAAGKIASVRYR
FGRYEQLDNSNNYGPMRQVDVAAQWPIARRWYAIGRYNYSFIERKPIERLAGFEYNDGCWSLRMYSQRYVTDPTTTKNAW
FFQLELKGLGALGNNGVQDTLRLAVPGYTKINE
>Mature_753_residues
MHRQKLNPLALALAAAFALNAPAALADDEEPPIAVPAPPKEPGQTVVHADDMDGEMSVILHAKGNVVATRDDQRVESDWL
DYYQTKNRVKAGDRFRMTRGGDVITGTTLDYNVDTYSGTGMDPVFSMARQNQTAVKPAPGAPPAKPVTLRGDGSQVDFQG
QNQYRVYGSRMTTCDPGDEAWYLKSSRTDLDYNTGVGVAHNAWMQFYGVPILYSPWLDFPLNSNRKSGFLMPTFKTGSSG
TEFSLPYYWNIAPNYDATITPHINVKHGNMLAGEFRYLQPDYSGRIYTEQLPKDKLTGESRYAWSASHSQNFGHGLSFGM
DFNQVSDNNYFTDFGDQVAIASNVNLNREAWLNYALGWQGGGGNVTLRAQRYQNLTINPVPGDIPYAKMPQLTFNANQSL
PSGFSANLISDLTRFDHPSLQNAERLVLYPSVSWNFDRSWGFLRPKLGVNYTHYNLDAFQGSPSHVQTRTLPIFSTDAGL
YFDRDTQFLGRDHLMTLEPRLFYVNIPNNRDQNSLPMFDTSVNDINFAQLFTENRYSGYDRINGANQITTALTSRFIDQS
NGLERLRLAVGKRFYLKDDVTQTVNQPSSDLLLSAGGDLTREWRFDSSYQYNQQLGMTERYNAQLRYNPAAGKIASVRYR
FGRYEQLDNSNNYGPMRQVDVAAQWPIARRWYAIGRYNYSFIERKPIERLAGFEYNDGCWSLRMYSQRYVTDPTTTKNAW
FFQLELKGLGALGNNGVQDTLRLAVPGYTKINE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=642, Percent_Identity=29.1277258566978, Blast_Score=253, Evalue=4e-68,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_CHRVO (Q7NQB1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903899.1
- GeneID:   2549530
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4229
- NMPDR:   fig|243365.1.peg.4229
- HOGENOM:   HBG701380
- OMA:   TPYYWNI
- PhylomeDB:   Q7NQB1
- ProtClustDB:   CLSK2485717
- BioCyc:   CVIO243365:CV_4229-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR007543

Pfam domain/function: PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 85097; Mature: 85097

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRQKLNPLALALAAAFALNAPAALADDEEPPIAVPAPPKEPGQTVVHADDMDGEMSVIL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCEEEEE
HAKGNVVATRDDQRVESDWLDYYQTKNRVKAGDRFRMTRGGDVITGTTLDYNVDTYSGTG
EECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCEEEEECCCCCCCC
MDPVFSMARQNQTAVKPAPGAPPAKPVTLRGDGSQVDFQGQNQYRVYGSRMTTCDPGDEA
CCHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEECCEEECCCCCCCE
WYLKSSRTDLDYNTGVGVAHNAWMQFYGVPILYSPWLDFPLNSNRKSGFLMPTFKTGSSG
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCC
TEFSLPYYWNIAPNYDATITPHINVKHGNMLAGEFRYLQPDYSGRIYTEQLPKDKLTGES
CEEECCEEEECCCCCCCEEEEEEEEECCCEEECEEEEECCCCCCEEECCCCCCCCCCCCC
RYAWSASHSQNFGHGLSFGMDFNQVSDNNYFTDFGDQVAIASNVNLNREAWLNYALGWQG
CEEEECCCCCCCCCCEECCCCCCCCCCCCCEECCCCEEEEEECCCCCHHHHEEEEEEEEC
GGGNVTLRAQRYQNLTINPVPGDIPYAKMPQLTFNANQSLPSGFSANLISDLTRFDHPSL
CCCCEEEEEECCCCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCC
QNAERLVLYPSVSWNFDRSWGFLRPKLGVNYTHYNLDAFQGSPSHVQTRTLPIFSTDAGL
CCCCEEEEEECCCCCCCCCCCEECCCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCE
YFDRDTQFLGRDHLMTLEPRLFYVNIPNNRDQNSLPMFDTSVNDINFAQLFTENRYSGYD
EEECCCHHCCCCCEEEECCEEEEEECCCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCH
RINGANQITTALTSRFIDQSNGLERLRLAVGKRFYLKDDVTQTVNQPSSDLLLSAGGDLT
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECHHHHHHCCCCCCEEEECCCCCC
REWRFDSSYQYNQQLGMTERYNAQLRYNPAAGKIASVRYRFGRYEQLDNSNNYGPMRQVD
EEEECCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCHHHCCCCCCCCCCEEEE
VAAQWPIARRWYAIGRYNYSFIERKPIERLAGFEYNDGCWSLRMYSQRYVTDPTTTKNAW
EEECCCCHHEEEEEECCCCHHHCCCCHHHHHCCCCCCCEEEEEEECCEEECCCCCCCCEE
FFQLELKGLGALGNNGVQDTLRLAVPGYTKINE
EEEEEEEECCCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MHRQKLNPLALALAAAFALNAPAALADDEEPPIAVPAPPKEPGQTVVHADDMDGEMSVIL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCEEEEE
HAKGNVVATRDDQRVESDWLDYYQTKNRVKAGDRFRMTRGGDVITGTTLDYNVDTYSGTG
EECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCEEEEECCCCCCCC
MDPVFSMARQNQTAVKPAPGAPPAKPVTLRGDGSQVDFQGQNQYRVYGSRMTTCDPGDEA
CCHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEECCEEECCCCCCCE
WYLKSSRTDLDYNTGVGVAHNAWMQFYGVPILYSPWLDFPLNSNRKSGFLMPTFKTGSSG
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCC
TEFSLPYYWNIAPNYDATITPHINVKHGNMLAGEFRYLQPDYSGRIYTEQLPKDKLTGES
CEEECCEEEECCCCCCCEEEEEEEEECCCEEECEEEEECCCCCCEEECCCCCCCCCCCCC
RYAWSASHSQNFGHGLSFGMDFNQVSDNNYFTDFGDQVAIASNVNLNREAWLNYALGWQG
CEEEECCCCCCCCCCEECCCCCCCCCCCCCEECCCCEEEEEECCCCCHHHHEEEEEEEEC
GGGNVTLRAQRYQNLTINPVPGDIPYAKMPQLTFNANQSLPSGFSANLISDLTRFDHPSL
CCCCEEEEEECCCCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCC
QNAERLVLYPSVSWNFDRSWGFLRPKLGVNYTHYNLDAFQGSPSHVQTRTLPIFSTDAGL
CCCCEEEEEECCCCCCCCCCCEECCCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCE
YFDRDTQFLGRDHLMTLEPRLFYVNIPNNRDQNSLPMFDTSVNDINFAQLFTENRYSGYD
EEECCCHHCCCCCEEEECCEEEEEECCCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCH
RINGANQITTALTSRFIDQSNGLERLRLAVGKRFYLKDDVTQTVNQPSSDLLLSAGGDLT
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECHHHHHHCCCCCCEEEECCCCCC
REWRFDSSYQYNQQLGMTERYNAQLRYNPAAGKIASVRYRFGRYEQLDNSNNYGPMRQVD
EEEECCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCHHHCCCCCCCCCCEEEE
VAAQWPIARRWYAIGRYNYSFIERKPIERLAGFEYNDGCWSLRMYSQRYVTDPTTTKNAW
EEECCCCHHEEEEEECCCCHHHCCCCHHHHHCCCCCCCEEEEEEECCEEECCCCCCCCEE
FFQLELKGLGALGNNGVQDTLRLAVPGYTKINE
EEEEEEEECCCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14500782