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The map label for this gene is rpoH [H]

Identifier: 34499661

GI number: 34499661

Start: 4537849

End: 4538712

Strand: Direct

Name: rpoH [H]

Synonym: CV_4206

Alternate gene names: 34499661

Gene position: 4537849-4538712 (Clockwise)

Preceding gene: 34499660

Following gene: 34499665

Centisome position: 95.51

GC content: 64.58

Gene sequence:

>864_bases
ATGAACACAACATTTGCCCTTCCGGTCCCGTCGACCAGCGGCAGCCTGGAACAATACATCCAGGCCGTGAACAGCATCCC
CATGCTGACGCCGGAACAGGAAACCGAGCTCGCGACCCGTTTCCAGCAGGAAAACGACCTGGAAGCGGCGCGGCAGATGG
TGCTGTCGCACCTGCGCGTGGTGGTGTCCGTGTCGCGCGGTTACGCCGGCTACGGCCTGCAACAGGCCGACCTGATCCAG
GAAGGCAATATCGGCCTGATGAAGGCGGTGAAGCGTTTCGAACCGGGCCGCGGCGTGCGTCTGTTCTCGTTCGCCATCCA
CTGGATCAAGGCCGAGATCCACGAATTCATCCTGCGCAACTGGCGCCTGGTGCGCATCGCCACCACCAAGCCGCAGCGCA
AGCTGTTCTTCAACCTGCGCAGCATGAAGAGCGGTTTTTCGGCGCTGTCGGAGCAGGAAGCCAAGTCCATCGCCGAAGAC
TTGGGCGTCAAGCCCGAGGAAGTGCGCGAAATGGAAATCCGCATGACCGGCCAGGACGTGTCCCTGCTGGCCGACAGCGA
CGACGAGGACAGCTACGCGCCAATCGACTGGCTGGCCGACAGCCACAGCGAGCCAACCCGCGCGCTGGAGCGACGCGCCT
TCGACATGCTGCAGTCGGAAGGCCTGGAATACGCGCTGTCGACCCTGGATCCGCGCAGCCGCCGCATCGTCGAGGCCCGC
TGGCTGGCCGACGACAGCGGCGCCACGCTGCACGAGCTGGCCGCCGAGTTCGGCGTATCCGCGGAACGCATCCGCCAGAT
CGAAGCCAAGGCGCTGTCCAAGATGAAGGCCGCGCTCAGCGAAGGCGTGGTGCTGGACAACTGA

Upstream 100 bases:

>100_bases
GGGGCCATTGTTAAATATCATTGACTCGGGAACCTTTCGCTGCTAGCACTCTCAATATTCGAGTGCTAAGATAGCACTAC
CCAAGGAGGTAGCCATCGCA

Downstream 100 bases:

>100_bases
TTCCACCCGTCCGTCGCAAAAAAAGCCACTCGAAACATCGAGCGGCTTTTTTCATGCCCTCCCCCGCCGATAGGGACGGG
GGAACGCGCCTCACTCCTGC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNTTFALPVPSTSGSLEQYIQAVNSIPMLTPEQETELATRFQQENDLEAARQMVLSHLRVVVSVSRGYAGYGLQQADLIQ
EGNIGLMKAVKRFEPGRGVRLFSFAIHWIKAEIHEFILRNWRLVRIATTKPQRKLFFNLRSMKSGFSALSEQEAKSIAED
LGVKPEEVREMEIRMTGQDVSLLADSDDEDSYAPIDWLADSHSEPTRALERRAFDMLQSEGLEYALSTLDPRSRRIVEAR
WLADDSGATLHELAAEFGVSAERIRQIEAKALSKMKAALSEGVVLDN

Sequences:

>Translated_287_residues
MNTTFALPVPSTSGSLEQYIQAVNSIPMLTPEQETELATRFQQENDLEAARQMVLSHLRVVVSVSRGYAGYGLQQADLIQ
EGNIGLMKAVKRFEPGRGVRLFSFAIHWIKAEIHEFILRNWRLVRIATTKPQRKLFFNLRSMKSGFSALSEQEAKSIAED
LGVKPEEVREMEIRMTGQDVSLLADSDDEDSYAPIDWLADSHSEPTRALERRAFDMLQSEGLEYALSTLDPRSRRIVEAR
WLADDSGATLHELAAEFGVSAERIRQIEAKALSKMKAALSEGVVLDN
>Mature_287_residues
MNTTFALPVPSTSGSLEQYIQAVNSIPMLTPEQETELATRFQQENDLEAARQMVLSHLRVVVSVSRGYAGYGLQQADLIQ
EGNIGLMKAVKRFEPGRGVRLFSFAIHWIKAEIHEFILRNWRLVRIATTKPQRKLFFNLRSMKSGFSALSEQEAKSIAED
LGVKPEEVREMEIRMTGQDVSLLADSDDEDSYAPIDWLADSHSEPTRALERRAFDMLQSEGLEYALSTLDPRSRRIVEAR
WLADDSGATLHELAAEFGVSAERIRQIEAKALSKMKAALSEGVVLDN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=269, Percent_Identity=51.3011152416357, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1789098, Length=266, Percent_Identity=31.9548872180451, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789448, Length=267, Percent_Identity=31.8352059925094, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32241; Mature: 32241

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTFALPVPSTSGSLEQYIQAVNSIPMLTPEQETELATRFQQENDLEAARQMVLSHLRV
CCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVSVSRGYAGYGLQQADLIQEGNIGLMKAVKRFEPGRGVRLFSFAIHWIKAEIHEFILRN
HHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WRLVRIATTKPQRKLFFNLRSMKSGFSALSEQEAKSIAEDLGVKPEEVREMEIRMTGQDV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCE
SLLADSDDEDSYAPIDWLADSHSEPTRALERRAFDMLQSEGLEYALSTLDPRSRRIVEAR
EEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHH
WLADDSGATLHELAAEFGVSAERIRQIEAKALSKMKAALSEGVVLDN
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTTFALPVPSTSGSLEQYIQAVNSIPMLTPEQETELATRFQQENDLEAARQMVLSHLRV
CCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVSVSRGYAGYGLQQADLIQEGNIGLMKAVKRFEPGRGVRLFSFAIHWIKAEIHEFILRN
HHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WRLVRIATTKPQRKLFFNLRSMKSGFSALSEQEAKSIAEDLGVKPEEVREMEIRMTGQDV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCE
SLLADSDDEDSYAPIDWLADSHSEPTRALERRAFDMLQSEGLEYALSTLDPRSRRIVEAR
EEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHH
WLADDSGATLHELAAEFGVSAERIRQIEAKALSKMKAALSEGVVLDN
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]