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The map label for this gene is speE

Identifier: 34499585

GI number: 34499585

Start: 4470273

End: 4471058

Strand: Reverse

Name: speE

Synonym: CV_4130

Alternate gene names: 34499585

Gene position: 4471058-4470273 (Counterclockwise)

Preceding gene: 34499586

Following gene: 34499584

Centisome position: 94.11

GC content: 64.25

Gene sequence:

>786_bases
CTGCATCCGTTCCGCCGCCGCGTGCGGCCGGCGGTGGAGGCGATGCCGGAGGTTGAGATCTCGGAAGAGGGCAACATCCG
CTCGCTGCACTTGGGCTCGGAAACGATACAGAGCTCGATGGACCTCGACGATCCGCCGGACCTGGTGCTGTCCTACAGCC
GCGCGATGATGGGCTTTCTGCTGTGGAACGACGATCCCAAGCACATCCTGCAGATCGGCCTGGGCGGCGGTTCCTTCGCC
CGCTTCATCGACGAATACCTGCCGGACGCGGTCAGCGTGGCGGTGGACATCAATCCGCAGGTGATCGCGGTGGCGCGCGC
CTTTTTCCAGTTGCCGGAGGAGGGCGATTTCTTCGAGATCGTCGAAGCCGACGGCGCCGACTACGTGAAGATCTTCCGCG
GCTCCACCGACGCCATCCTGGTGGACGGCTTCGACGGCCTGCAGATCGTCGACGCGTTGACGACCGAGGACTTCTTCGAG
GACTGCAAGCGCGCGCTGAGTCCCAAGGGCGTGTTCGTCACCAACTGGTGGAGCGGCGACAAGCGCTACCAGTCCTTCAT
CGAGCGCTTGTTGAACGTTTTCGAGGGCCGTGTGATCGAACTGCCTGCCGCCACCCACGGCAACGTCGCGGTGATGGCCT
TCCGCCAGAGCCCGAGCCTGACGCAGTGGGAGGCGCTGAGAAAACGCGCCGACGAGCTGGAAGGGCGTTTCGGGCTGGAG
TTCGGCGAGTTCGTCAACCGGCTGAAGCAGACCAATCAGCAGACTGCCGGCCACCTGCTGATCTGA

Upstream 100 bases:

>100_bases
CCGGCGCCGGACGCGGACGATGTGGAGGACGCGCCGAAAAAACGCACCATCAGCATTCTGGATTCGCGCGGCAAGGAGAG
CGGCTGTGGCGCGTAGGGCG

Downstream 100 bases:

>100_bases
GCCAGGACAAATCCGCCGCGGAAAGCGCGCGGCGGTTACGTTTGCTTACCCAAAAACCGGGTCGACGCGGCGTATAATGC
CAAGTTGCGCGCGCATTGGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MHPFRRRVRPAVEAMPEVEISEEGNIRSLHLGSETIQSSMDLDDPPDLVLSYSRAMMGFLLWNDDPKHILQIGLGGGSFA
RFIDEYLPDAVSVAVDINPQVIAVARAFFQLPEEGDFFEIVEADGADYVKIFRGSTDAILVDGFDGLQIVDALTTEDFFE
DCKRALSPKGVFVTNWWSGDKRYQSFIERLLNVFEGRVIELPAATHGNVAVMAFRQSPSLTQWEALRKRADELEGRFGLE
FGEFVNRLKQTNQQTAGHLLI

Sequences:

>Translated_261_residues
MHPFRRRVRPAVEAMPEVEISEEGNIRSLHLGSETIQSSMDLDDPPDLVLSYSRAMMGFLLWNDDPKHILQIGLGGGSFA
RFIDEYLPDAVSVAVDINPQVIAVARAFFQLPEEGDFFEIVEADGADYVKIFRGSTDAILVDGFDGLQIVDALTTEDFFE
DCKRALSPKGVFVTNWWSGDKRYQSFIERLLNVFEGRVIELPAATHGNVAVMAFRQSPSLTQWEALRKRADELEGRFGLE
FGEFVNRLKQTNQQTAGHLLI
>Mature_261_residues
MHPFRRRVRPAVEAMPEVEISEEGNIRSLHLGSETIQSSMDLDDPPDLVLSYSRAMMGFLLWNDDPKHILQIGLGGGSFA
RFIDEYLPDAVSVAVDINPQVIAVARAFFQLPEEGDFFEIVEADGADYVKIFRGSTDAILVDGFDGLQIVDALTTEDFFE
DCKRALSPKGVFVTNWWSGDKRYQSFIERLLNVFEGRVIELPAATHGNVAVMAFRQSPSLTQWEALRKRADELEGRFGLE
FGEFVNRLKQTNQQTAGHLLI

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_CHRVO (Q7NQK7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903800.1
- ProteinModelPortal:   Q7NQK7
- SMR:   Q7NQK7
- GeneID:   2549078
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4130
- NMPDR:   fig|243365.1.peg.4130
- HOGENOM:   HBG752555
- OMA:   SWLEYDY
- ProtClustDB:   PRK04457
- BioCyc:   CVIO243365:CV_4130-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 29357; Mature: 29357

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 27-27 BINDING 96-96 BINDING 142-142 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPFRRRVRPAVEAMPEVEISEEGNIRSLHLGSETIQSSMDLDDPPDLVLSYSRAMMGFL
CCCHHHHHHHHHHHCCCCEECCCCCEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LWNDDPKHILQIGLGGGSFARFIDEYLPDAVSVAVDINPQVIAVARAFFQLPEEGDFFEI
EECCCHHHEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEE
VEADGADYVKIFRGSTDAILVDGFDGLQIVDALTTEDFFEDCKRALSPKGVFVTNWWSGD
EECCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KRYQSFIERLLNVFEGRVIELPAATHGNVAVMAFRQSPSLTQWEALRKRADELEGRFGLE
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
FGEFVNRLKQTNQQTAGHLLI
HHHHHHHHHHHCHHCCCCCCC
>Mature Secondary Structure
MHPFRRRVRPAVEAMPEVEISEEGNIRSLHLGSETIQSSMDLDDPPDLVLSYSRAMMGFL
CCCHHHHHHHHHHHCCCCEECCCCCEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LWNDDPKHILQIGLGGGSFARFIDEYLPDAVSVAVDINPQVIAVARAFFQLPEEGDFFEI
EECCCHHHEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEE
VEADGADYVKIFRGSTDAILVDGFDGLQIVDALTTEDFFEDCKRALSPKGVFVTNWWSGD
EECCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KRYQSFIERLLNVFEGRVIELPAATHGNVAVMAFRQSPSLTQWEALRKRADELEGRFGLE
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
FGEFVNRLKQTNQQTAGHLLI
HHHHHHHHHHHCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782