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The map label for this gene is wcaJ [H]

Identifier: 34499572

GI number: 34499572

Start: 4457627

End: 4458220

Strand: Reverse

Name: wcaJ [H]

Synonym: CV_4117

Alternate gene names: 34499572

Gene position: 4458220-4457627 (Counterclockwise)

Preceding gene: 34499573

Following gene: 34499571

Centisome position: 93.84

GC content: 65.82

Gene sequence:

>594_bases
CTGTTGAAGCGGCTGTTCGACATCGTGGCTTCCGGCCTGGGTCTCGCCGTGCTGGCGCTGCCGCTGCTGGCGGTGGCGCT
GTGGGTGAAGCTGGACTCGCCGGGGCCGGTATTCTTCCGCCAGGTGCGGGTGGGGCGAGGCGGCACGCTGTTCCGCATCC
ACAAGTTCCGCACCATGCAGGTGGACACCGAGCGCCACGGCCAACTGACCGTCGGCGCCGATGCGCGCATCACCGGCGCC
GGCCGCCTGCTGCGCAAGACCAAGCTGGACGAGCTGCCGCAGCTGATCGACGTGCTGTTCGGCGACATGAGCCTGGTCGG
TCCGCGGCCGGAGGTGCCGAAGTACGTGGCGCACTATCCTGACGAGGCGAGGGACATCGTGCTGTCGGTGCGCCCCGGCA
TCACCGACTGGGCGTCGATCCGGATGATCGATGAAAACGAGATACTGGGCCGCGCCGCCGATCCGGAGCGCGCCTATATC
GAAGAAGTCCTGCCTGAGAAGCTGGCTTATTACGTCCGTTACGCCGAGACGCACAGTCTGTGGGAAGACATCCGCATCAT
CGTCGCCACCTTGATGAAGATCGTCAGCCGATGA

Upstream 100 bases:

>100_bases
CCGCGGTGCGCGAGATTCTGGGCGCATGAGCGAGCGGCTGGAAAAAAAGGGCGGCGAATTGCTGCCGCCCTGCTGCCGGC
GGCGCCGCCACGGCTCGCGG

Downstream 100 bases:

>100_bases
CCTTCTCCATCCGCGCCGCCCAAGCCGCCGACGCTGGCGCGCTGGCGGCCATTTACCTGGCCTGCCGCAGCGAGATGCCG
TACGCGCCGCTGGCGCACGG

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLKRLFDIVASGLGLAVLALPLLAVALWVKLDSPGPVFFRQVRVGRGGTLFRIHKFRTMQVDTERHGQLTVGADARITGA
GRLLRKTKLDELPQLIDVLFGDMSLVGPRPEVPKYVAHYPDEARDIVLSVRPGITDWASIRMIDENEILGRAADPERAYI
EEVLPEKLAYYVRYAETHSLWEDIRIIVATLMKIVSR

Sequences:

>Translated_197_residues
MLKRLFDIVASGLGLAVLALPLLAVALWVKLDSPGPVFFRQVRVGRGGTLFRIHKFRTMQVDTERHGQLTVGADARITGA
GRLLRKTKLDELPQLIDVLFGDMSLVGPRPEVPKYVAHYPDEARDIVLSVRPGITDWASIRMIDENEILGRAADPERAYI
EEVLPEKLAYYVRYAETHSLWEDIRIIVATLMKIVSR
>Mature_197_residues
MLKRLFDIVASGLGLAVLALPLLAVALWVKLDSPGPVFFRQVRVGRGGTLFRIHKFRTMQVDTERHGQLTVGADARITGA
GRLLRKTKLDELPQLIDVLFGDMSLVGPRPEVPKYVAHYPDEARDIVLSVRPGITDWASIRMIDENEILGRAADPERAYI
EEVLPEKLAYYVRYAETHSLWEDIRIIVATLMKIVSR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=196, Percent_Identity=38.265306122449, Blast_Score=119, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 22200; Mature: 22200

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLFDIVASGLGLAVLALPLLAVALWVKLDSPGPVFFRQVRVGRGGTLFRIHKFRTMQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHEEEEECCCCCEEEEEEEEEEE
VDTERHGQLTVGADARITGAGRLLRKTKLDELPQLIDVLFGDMSLVGPRPEVPKYVAHYP
ECCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHCCC
DEARDIVLSVRPGITDWASIRMIDENEILGRAADPERAYIEEVLPEKLAYYVRYAETHSL
CCHHEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WEDIRIIVATLMKIVSR
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKRLFDIVASGLGLAVLALPLLAVALWVKLDSPGPVFFRQVRVGRGGTLFRIHKFRTMQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHEEEEECCCCCEEEEEEEEEEE
VDTERHGQLTVGADARITGAGRLLRKTKLDELPQLIDVLFGDMSLVGPRPEVPKYVAHYP
ECCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHCCC
DEARDIVLSVRPGITDWASIRMIDENEILGRAADPERAYIEEVLPEKLAYYVRYAETHSL
CCHHEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WEDIRIIVATLMKIVSR
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]