Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 34499567

GI number: 34499567

Start: 4452108

End: 4453511

Strand: Reverse

Name: aspA [H]

Synonym: CV_4112

Alternate gene names: 34499567

Gene position: 4453511-4452108 (Counterclockwise)

Preceding gene: 34499568

Following gene: 34499565

Centisome position: 93.74

GC content: 67.59

Gene sequence:

>1404_bases
ATGTCCACCCGTATCGAACACGACCTGCTCGGCAACCGCGACGTGCCGGCCGACGCCTACTGGGGCGTGCACACGCTGCG
CGCCATCGAAAACTTCCCGATCACCGGCCAGACCATCGCCGGCTACGGCGACCTGATCCGCGCGCTGGCCTGCATCAAGC
AAGCCGCCGCCCAGGCCAACCGCGATCTCGGCATTCTCGACCCCAAGCTGGCCCAGGCCATCGTCGACGCCTGCGAGGAG
ATCCGCGCCGGCAAGCTGCACGACCAGTTCGTCGTCGACGTGATCCAGGGCGGCGCCGGCACTTCCACCAATATGAACGC
CAACGAGGTGATCGCCAACCGCGCGCTGGAGCTGCTGGGCCACGCCAAGGGCGAGTACCAGCATCTGCACCCGAACGAGC
ACGTCAACCTGTGCCAGAGCACCAACGACGTCTACCCGACCGCGCTGCGCCTGGCCGCCTTCTGGGGCGTGCTGAAGCTG
GTGTCGCGGATGGAAACGCTGCGCAAGGCCTTCGAGGACAAGGCCGAGGAATTCAAGGACATCCTGAAGATGGGCCGCAC
CCAGCTGCAGGACGCGGTGCCGATGACGCTGGGCCAGGAGTTCCAGACCTATGCTGTGATGCTGGGCGAGGACGAGCAGC
GTCTGAAGGAAGCCAGCGCCCTGATGCTGGAGATCAACCTCGGCGCCACCGCGATCGGCACCGGCATCACCGCCCACCCG
GACTACGCCAAGCTGGTGTGCCTGCATCTGTCCGAGCTGGTGGGCAGCCAGCTGATCACCGCGCCCAACCTGATCGAGGC
CACCCAGGACTGCGGCGCCTTCGTCCAGCTGTCCGGCGTGATCAAGCGCGCGGCGGTCAAACTGTCCAAGACCTGCAACG
ACCTGCGCCTGCTGTCGTCCGGCCCGCGCGCAGGCTTCGGCGAGATCAATCTGCCGGCGCGCCAGGCCGGCTCGTCCATC
ATGCCGGGCAAGGTCAATCCGGTGATTCCGGAAGTGGTGAGCCAGGTGGCGTATGAAGTCATCGGCAACGACGTCACCAT
CACCATGGCGGCCGAGGCCGGCCAGCTGCAGCTGAACGCCTTCGAGCCGGTGATCGCCTACAGCCTGTTCCGCAGCGCCG
GCCACCTGGCCAACGCCTGCGACACGCTGACCGAGCACTGCGTCAAGGGCATCACCGCCAACCGCGAGCGGCTGCGCCTG
AGCGTGGAAAGCTCGATCGGCCTGGTCACCGCGCTGAACCCGGTGATCGGCTACGAGGCCGCCACCCGCGTCGCCGCCGA
AGCCCACGCCAACGGCAGCAGCGTCGCCGACGTGGTGCTGGCCCATGGCCTGCTCACCCGCGAGCAGCTGGACGACATCC
TGCAGCCGGAGACCCTGACCCGGCCGCGCTGGGTGACGCTGTAA

Upstream 100 bases:

>100_bases
GCATGACCCCATCGCCATTCGGAATTGGCCAAGCTTCCGGGGCGGGCATACAGTGCGGGGCATTGCAGGCCGCGCATGGC
GGCCGCCCTGAGGAGACGAG

Downstream 100 bases:

>100_bases
ACCGCTTTCCCATCCTTTGTTTGACTCGTGCTTGCCGGCGCCTTGAGCGCCGGTTTTTTTTCCCCGCCTTCGCGACGAGG
CGGCGATTGCGCGCGCTATT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSTRIEHDLLGNRDVPADAYWGVHTLRAIENFPITGQTIAGYGDLIRALACIKQAAAQANRDLGILDPKLAQAIVDACEE
IRAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLGHAKGEYQHLHPNEHVNLCQSTNDVYPTALRLAAFWGVLKL
VSRMETLRKAFEDKAEEFKDILKMGRTQLQDAVPMTLGQEFQTYAVMLGEDEQRLKEASALMLEINLGATAIGTGITAHP
DYAKLVCLHLSELVGSQLITAPNLIEATQDCGAFVQLSGVIKRAAVKLSKTCNDLRLLSSGPRAGFGEINLPARQAGSSI
MPGKVNPVIPEVVSQVAYEVIGNDVTITMAAEAGQLQLNAFEPVIAYSLFRSAGHLANACDTLTEHCVKGITANRERLRL
SVESSIGLVTALNPVIGYEAATRVAAEAHANGSSVADVVLAHGLLTREQLDDILQPETLTRPRWVTL

Sequences:

>Translated_467_residues
MSTRIEHDLLGNRDVPADAYWGVHTLRAIENFPITGQTIAGYGDLIRALACIKQAAAQANRDLGILDPKLAQAIVDACEE
IRAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLGHAKGEYQHLHPNEHVNLCQSTNDVYPTALRLAAFWGVLKL
VSRMETLRKAFEDKAEEFKDILKMGRTQLQDAVPMTLGQEFQTYAVMLGEDEQRLKEASALMLEINLGATAIGTGITAHP
DYAKLVCLHLSELVGSQLITAPNLIEATQDCGAFVQLSGVIKRAAVKLSKTCNDLRLLSSGPRAGFGEINLPARQAGSSI
MPGKVNPVIPEVVSQVAYEVIGNDVTITMAAEAGQLQLNAFEPVIAYSLFRSAGHLANACDTLTEHCVKGITANRERLRL
SVESSIGLVTALNPVIGYEAATRVAAEAHANGSSVADVVLAHGLLTREQLDDILQPETLTRPRWVTL
>Mature_466_residues
STRIEHDLLGNRDVPADAYWGVHTLRAIENFPITGQTIAGYGDLIRALACIKQAAAQANRDLGILDPKLAQAIVDACEEI
RAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLGHAKGEYQHLHPNEHVNLCQSTNDVYPTALRLAAFWGVLKLV
SRMETLRKAFEDKAEEFKDILKMGRTQLQDAVPMTLGQEFQTYAVMLGEDEQRLKEASALMLEINLGATAIGTGITAHPD
YAKLVCLHLSELVGSQLITAPNLIEATQDCGAFVQLSGVIKRAAVKLSKTCNDLRLLSSGPRAGFGEINLPARQAGSSIM
PGKVNPVIPEVVSQVAYEVIGNDVTITMAAEAGQLQLNAFEPVIAYSLFRSAGHLANACDTLTEHCVKGITANRERLRLS
VESSIGLVTALNPVIGYEAATRVAAEAHANGSSVADVVLAHGLLTREQLDDILQPETLTRPRWVTL

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=44.2764578833693, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI87082375, Length=464, Percent_Identity=57.3275862068966, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=42.7015250544662, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=42.7331887201735, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=41.4634146341463, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=42.3326133909287, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=42.6406926406926, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=42.6406926406926, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=41.2903225806452, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=41.036717062635, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=37.5809935205184, Blast_Score=333, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50319; Mature: 50188

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRIEHDLLGNRDVPADAYWGVHTLRAIENFPITGQTIAGYGDLIRALACIKQAAAQAN
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RDLGILDPKLAQAIVDACEEIRAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
HAKGEYQHLHPNEHVNLCQSTNDVYPTALRLAAFWGVLKLVSRMETLRKAFEDKAEEFKD
CCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKMGRTQLQDAVPMTLGQEFQTYAVMLGEDEQRLKEASALMLEINLGATAIGTGITAHP
HHHHHHHHHHHHCCHHHCCCHHHHHEEECCCHHHHHHHHEEEEEEECCCCHHCCCCCCCC
DYAKLVCLHLSELVGSQLITAPNLIEATQDCGAFVQLSGVIKRAAVKLSKTCNDLRLLSS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPRAGFGEINLPARQAGSSIMPGKVNPVIPEVVSQVAYEVIGNDVTITMAAEAGQLQLNA
CCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECC
FEPVIAYSLFRSAGHLANACDTLTEHCVKGITANRERLRLSVESSIGLVTALNPVIGYEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHHCCCHHHHHHHHHHHHH
ATRVAAEAHANGSSVADVVLAHGLLTREQLDDILQPETLTRPRWVTL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
STRIEHDLLGNRDVPADAYWGVHTLRAIENFPITGQTIAGYGDLIRALACIKQAAAQAN
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RDLGILDPKLAQAIVDACEEIRAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
HAKGEYQHLHPNEHVNLCQSTNDVYPTALRLAAFWGVLKLVSRMETLRKAFEDKAEEFKD
CCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKMGRTQLQDAVPMTLGQEFQTYAVMLGEDEQRLKEASALMLEINLGATAIGTGITAHP
HHHHHHHHHHHHCCHHHCCCHHHHHEEECCCHHHHHHHHEEEEEEECCCCHHCCCCCCCC
DYAKLVCLHLSELVGSQLITAPNLIEATQDCGAFVQLSGVIKRAAVKLSKTCNDLRLLSS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPRAGFGEINLPARQAGSSIMPGKVNPVIPEVVSQVAYEVIGNDVTITMAAEAGQLQLNA
CCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECC
FEPVIAYSLFRSAGHLANACDTLTEHCVKGITANRERLRLSVESSIGLVTALNPVIGYEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHHCCCHHHHHHHHHHHHH
ATRVAAEAHANGSSVADVVLAHGLLTREQLDDILQPETLTRPRWVTL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]