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The map label for this gene is algI [H]

Identifier: 34499521

GI number: 34499521

Start: 4396483

End: 4397877

Strand: Reverse

Name: algI [H]

Synonym: CV_4066

Alternate gene names: 34499521

Gene position: 4397877-4396483 (Counterclockwise)

Preceding gene: 34499522

Following gene: 34499520

Centisome position: 92.57

GC content: 66.31

Gene sequence:

>1395_bases
CTGTCGATTGAATTCGCCCTGGCCTTCGTCGGTTTTTTTACCGTCTACTGGGGCTGCCGCCGCATGCCCAGCATGCAGAA
CCTTCTGCTCCTGCTGGCCAGCTATTTGTTCTACGCCAAGCTGGACTGGCGCTTCGCCTCCATCCTCGCCGCCTATACGC
TGATCCAGCACGGCCTCAGCCGGAGGATGACGGCGTCGGCCCGTCCCAGGCGCTGGCTGCAGGCCTCGCTCGCGCTGGCG
GTGGGCAATCTGGCGCTGTTCAAGTATTTCGACTTTTTCCGCGAAAGCGCGCAGGCCGGCCTCAATGCGCTGGGGGTGAC
GGCGATGCTGCCCGCGCTCGATATCCTGATGCCGGTGGGCATCTCCTTCTATACCTTCCAGTCGGTCAGCTACTTGGTGT
CGGTGTACCGCAAGGAAATCCGGCCGGCGGCGCTGCCGCAGTTCGCGCTGTTCCTGGCCTTTTTCCCCACCTTGCTGGCG
GGGCCGATCTGCCGCGCCGGCGACCTCTTGGAGCAGCTGCGCAGCCCGGTCCGGCGCCAGGTGGCGCATCTGGACCGCGC
CTTGGGCCTGATCATTCTGGCCTTGCTGAAGAAGCTGTGGCTGGCAGGCTGGCTGGCCAGCGCCTGGGTGGATCCGGTGT
TCGCCGCGCCCGACACCTTCCACCGCCTGGAGGTGTGGGCGGCCTTCTACGCCTACGCGTTGCAGATCTACCTGGACTTC
TCCGGCTATACCGACCTGGTCACCGCGCTGGCGCTGCTGCTGGGCATCCAGCTGCCGCGCAACTTTGATGCGCCTTACCT
GGCGCTGAACCTGCGCGATTTCTGGCGGCGCTGGCACATCTCGCTGTCCAGCTGGATCCGCGACTATGTCTATATCCCGC
TGGGCGGCAACCGCGGCGGCTTCGCCCGCGCCCAGATCAACCTGCTGGCGGCGATGCTGCTGTCCGGCCTGTGGCACGGC
GCCAGTCTGAAATACCTGGTGTGGGGCGCGATGCATGGCCTGGGCGTGGTGGCGCTCAACATCGGCGACAAGCTGTGGCG
CCGCGACGCCGTCAGCAGCGCGTCGCCATGGCTGGCGCGGCTGATCACCTTCCATTACGTGTGCCTGGCCTGGGTGTTCT
TCCGCGCGCAGTCGCTGGACGAGGCGATGGACTTCCTGCGCGCGATGTTCGCGCCGTCAGGCCAGGAGCTGACCTTGAAC
GCGCCGGGCCTGCTGCTGCTGCTGGGTTTGTGGCTGGCGGCGCTGCCGACGCTGTCCACGCTGCCCGAGCGCTGCTTCGG
CCGGATGGCGGTGCTGCCGTGGTGGGGCAAGCCGCTGTTGCTGAGCGCGATCGCGCTGCTGGTGATCTCGCTGGCGCCGT
CCGGCATTCCCGCCTTCATTTATTACCAATTCTGA

Upstream 100 bases:

>100_bases
GCAACAGGCAGATAAATAAATTTTTACCGGAGCTGGCATGCGGCGGGGCTTGTTGTCATAATTGCCGCCTCCGCCAGCTT
GTTACCTTAGCATGAGCTAT

Downstream 100 bases:

>100_bases
CGATCCGACCTTCGCCATGCTCGCCGCCTATCCGCACCGCCGCATCCTCGTCATCCTGCTCAGCTCCATGCTGCTGCTGT
TCTGGCTGGAACAGGCCGCA

Product: alginate O-acetylation protein AlgI

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSIEFALAFVGFFTVYWGCRRMPSMQNLLLLLASYLFYAKLDWRFASILAAYTLIQHGLSRRMTASARPRRWLQASLALA
VGNLALFKYFDFFRESAQAGLNALGVTAMLPALDILMPVGISFYTFQSVSYLVSVYRKEIRPAALPQFALFLAFFPTLLA
GPICRAGDLLEQLRSPVRRQVAHLDRALGLIILALLKKLWLAGWLASAWVDPVFAAPDTFHRLEVWAAFYAYALQIYLDF
SGYTDLVTALALLLGIQLPRNFDAPYLALNLRDFWRRWHISLSSWIRDYVYIPLGGNRGGFARAQINLLAAMLLSGLWHG
ASLKYLVWGAMHGLGVVALNIGDKLWRRDAVSSASPWLARLITFHYVCLAWVFFRAQSLDEAMDFLRAMFAPSGQELTLN
APGLLLLLGLWLAALPTLSTLPERCFGRMAVLPWWGKPLLLSAIALLVISLAPSGIPAFIYYQF

Sequences:

>Translated_464_residues
MSIEFALAFVGFFTVYWGCRRMPSMQNLLLLLASYLFYAKLDWRFASILAAYTLIQHGLSRRMTASARPRRWLQASLALA
VGNLALFKYFDFFRESAQAGLNALGVTAMLPALDILMPVGISFYTFQSVSYLVSVYRKEIRPAALPQFALFLAFFPTLLA
GPICRAGDLLEQLRSPVRRQVAHLDRALGLIILALLKKLWLAGWLASAWVDPVFAAPDTFHRLEVWAAFYAYALQIYLDF
SGYTDLVTALALLLGIQLPRNFDAPYLALNLRDFWRRWHISLSSWIRDYVYIPLGGNRGGFARAQINLLAAMLLSGLWHG
ASLKYLVWGAMHGLGVVALNIGDKLWRRDAVSSASPWLARLITFHYVCLAWVFFRAQSLDEAMDFLRAMFAPSGQELTLN
APGLLLLLGLWLAALPTLSTLPERCFGRMAVLPWWGKPLLLSAIALLVISLAPSGIPAFIYYQF
>Mature_463_residues
SIEFALAFVGFFTVYWGCRRMPSMQNLLLLLASYLFYAKLDWRFASILAAYTLIQHGLSRRMTASARPRRWLQASLALAV
GNLALFKYFDFFRESAQAGLNALGVTAMLPALDILMPVGISFYTFQSVSYLVSVYRKEIRPAALPQFALFLAFFPTLLAG
PICRAGDLLEQLRSPVRRQVAHLDRALGLIILALLKKLWLAGWLASAWVDPVFAAPDTFHRLEVWAAFYAYALQIYLDFS
GYTDLVTALALLLGIQLPRNFDAPYLALNLRDFWRRWHISLSSWIRDYVYIPLGGNRGGFARAQINLLAAMLLSGLWHGA
SLKYLVWGAMHGLGVVALNIGDKLWRRDAVSSASPWLARLITFHYVCLAWVFFRAQSLDEAMDFLRAMFAPSGQELTLNA
PGLLLLLGLWLAALPTLSTLPERCFGRMAVLPWWGKPLLLSAIALLVISLAPSGIPAFIYYQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 52118; Mature: 51987

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEFALAFVGFFTVYWGCRRMPSMQNLLLLLASYLFYAKLDWRFASILAAYTLIQHGLS
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRMTASARPRRWLQASLALAVGNLALFKYFDFFRESAQAGLNALGVTAMLPALDILMPVG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
ISFYTFQSVSYLVSVYRKEIRPAALPQFALFLAFFPTLLAGPICRAGDLLEQLRSPVRRQ
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VAHLDRALGLIILALLKKLWLAGWLASAWVDPVFAAPDTFHRLEVWAAFYAYALQIYLDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHEEC
SGYTDLVTALALLLGIQLPRNFDAPYLALNLRDFWRRWHISLSSWIRDYVYIPLGGNRGG
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
FARAQINLLAAMLLSGLWHGASLKYLVWGAMHGLGVVALNIGDKLWRRDAVSSASPWLAR
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHHH
LITFHYVCLAWVFFRAQSLDEAMDFLRAMFAPSGQELTLNAPGLLLLLGLWLAALPTLST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
LPERCFGRMAVLPWWGKPLLLSAIALLVISLAPSGIPAFIYYQF
HHHHHHCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
SIEFALAFVGFFTVYWGCRRMPSMQNLLLLLASYLFYAKLDWRFASILAAYTLIQHGLS
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRMTASARPRRWLQASLALAVGNLALFKYFDFFRESAQAGLNALGVTAMLPALDILMPVG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
ISFYTFQSVSYLVSVYRKEIRPAALPQFALFLAFFPTLLAGPICRAGDLLEQLRSPVRRQ
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VAHLDRALGLIILALLKKLWLAGWLASAWVDPVFAAPDTFHRLEVWAAFYAYALQIYLDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHEEC
SGYTDLVTALALLLGIQLPRNFDAPYLALNLRDFWRRWHISLSSWIRDYVYIPLGGNRGG
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
FARAQINLLAAMLLSGLWHGASLKYLVWGAMHGLGVVALNIGDKLWRRDAVSSASPWLAR
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHHH
LITFHYVCLAWVFFRAQSLDEAMDFLRAMFAPSGQELTLNAPGLLLLLGLWLAALPTLST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
LPERCFGRMAVLPWWGKPLLLSAIALLVISLAPSGIPAFIYYQF
HHHHHHCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]