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The map label for this gene is cls [H]

Identifier: 34499494

GI number: 34499494

Start: 4369548

End: 4371065

Strand: Direct

Name: cls [H]

Synonym: CV_4039

Alternate gene names: 34499494

Gene position: 4369548-4371065 (Clockwise)

Preceding gene: 34499472

Following gene: 34499495

Centisome position: 91.97

GC content: 66.8

Gene sequence:

>1518_bases
ATGAGCAAAGACGAGCAGTCGCAACAAACCCTGCAATTCGTCCGCCAGCTGGCGGAACAGGCGCTTTCGCGCTCCGCCGG
CGCGCCGCTGATGGCCGGAAACCGAGTGCGGCTGCTGTACGACAGCGTGGAAAACTTTCCCGCCTGGGAGAACGCGCTCT
CAGAATGCCGGGATTCCATCTTTATCGAAATGTATATATTCGCCAATGACCAATTTGGCTTGAAAATCCGTAATATCCTT
ATAGAAAAAGCAAAACAAGGCATCCAAGTTTGCCTGTTGTATGACTGGCTGGGCAGCTGGCGCGCGCACTTGTCCGGCTT
CCTCAAGCCGCTGCAGGAAGCCGGAGCCGAGGTGCGCGCCTACCATCCCCCCAGCCTGGGCGGCGGGCTCACGCTGTTGG
GGCGCAACCACCGCAAGATGATCATCGTCGACCGGCAAGAGCTGTTCGTTTCCGGGCTGTGCATCAGCCAGAGCTGGCAA
GGCCAGCCTGAAAAGGGACTGGCGCCATGGCGCGACACCGGCCTGGCGCTGAGCGGCCCGCTGCTGGCGCCGGCGCTGGC
GGCCTTTGCCGACAGCTGGGCCAGTTGCGGCGAGCCGCTGGACGCGCGCTGGCTGGTTCCGGCCGACACGCAGCCCGCCG
GCGAGGCCGCCGCGAGGTTGATCGCCACCACGCCTTCCACCGCGCGGATGATGCGGCTGGATCTGTTGGTGGCCAGCTTC
GCCCGCCGCACGCTGTGGCTGACCGACGCCTACTTCATGGCCACCAGCCTCTATCTGTCGGCGTTGAAGCAGGCGGCGCG
GGACGGCGTCGACGTGCGCCTCCTGGTGCCGCGCTCCAGCGACATCCGCTGGATCGCCGCCGTGTCCCGCACCCAGTACC
GCCCGCTGCTGGAAGCCGGGGTGCGGGTGTTCGAGTGGAACGGGCCGATGATCCACGCCAAAACCGCTGTCGCCGACGGC
CGCTGGGCCCGCATCGGCTCCACCAACCTCAATCTGTCCAGCTGGCTGGTCAACCGCGAACTGGACATCGCGCTGGAGGA
CGAGGGCCTGGCCGGAGAACTCGCGGAACGCTTCCTGCAGGACCTGGAACAGTCGACCGAGATCGTGCTGCGCCAGGAGC
GGCGCAAGCCGGTGCCGCGGCCGGTCAGCAAGCAGGAACGGCGGATGCGCGGCACGCCCAAGGAAATGGCGCTCAGCGGC
GCCAGCGCGGCGGCGCGCCAGGCGATGCGCATCGGCGACGCGCTGGGCTCCGTGGTGCGCGGCAGCCGCATGGTGGAAAG
CAGCGAGGCCCCGGCCTTCCTCAGCATAGGCGTCGCCCTGTGCCTGCTGGCGCTGGCCATCGCCTACTTTCCGTGGCTGG
CGGCCGCGCCGTTGGTCGCGCTGCTGGCCGCGGGCGGCCTGGCCGTGGTGTTCAAGGCCTGCGGGCTATATCTGGAAAAA
CGGAAGAAAAAACAACAGTCGAGGCTGGCGGAGTGCAGCAGCGGAAGCGACAAGCCGACGCCGGACGACTCTCAATGA

Upstream 100 bases:

>100_bases
AAAATTTTCAAAAATACGACAGTTTTTCCTACTCAAAATCAGATACAAAGAAGCCATTACGGCAATATTTTGTATTTTGT
TAGACAAAATTCAAAAAATC

Downstream 100 bases:

>100_bases
AAACAGCCGCTTAGCATATGGGCGCAGCTGTTTACGGCGCGCCAGCCGCCCTGGCCGGCGCCGGCAGATAGTCCTGATCG
CGATTTTTCCGCGATCCATT

Product: phosphatidylglycerol phosphate synthetase

Products: CMP; 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSKDEQSQQTLQFVRQLAEQALSRSAGAPLMAGNRVRLLYDSVENFPAWENALSECRDSIFIEMYIFANDQFGLKIRNIL
IEKAKQGIQVCLLYDWLGSWRAHLSGFLKPLQEAGAEVRAYHPPSLGGGLTLLGRNHRKMIIVDRQELFVSGLCISQSWQ
GQPEKGLAPWRDTGLALSGPLLAPALAAFADSWASCGEPLDARWLVPADTQPAGEAAARLIATTPSTARMMRLDLLVASF
ARRTLWLTDAYFMATSLYLSALKQAARDGVDVRLLVPRSSDIRWIAAVSRTQYRPLLEAGVRVFEWNGPMIHAKTAVADG
RWARIGSTNLNLSSWLVNRELDIALEDEGLAGELAERFLQDLEQSTEIVLRQERRKPVPRPVSKQERRMRGTPKEMALSG
ASAAARQAMRIGDALGSVVRGSRMVESSEAPAFLSIGVALCLLALAIAYFPWLAAAPLVALLAAGGLAVVFKACGLYLEK
RKKKQQSRLAECSSGSDKPTPDDSQ

Sequences:

>Translated_505_residues
MSKDEQSQQTLQFVRQLAEQALSRSAGAPLMAGNRVRLLYDSVENFPAWENALSECRDSIFIEMYIFANDQFGLKIRNIL
IEKAKQGIQVCLLYDWLGSWRAHLSGFLKPLQEAGAEVRAYHPPSLGGGLTLLGRNHRKMIIVDRQELFVSGLCISQSWQ
GQPEKGLAPWRDTGLALSGPLLAPALAAFADSWASCGEPLDARWLVPADTQPAGEAAARLIATTPSTARMMRLDLLVASF
ARRTLWLTDAYFMATSLYLSALKQAARDGVDVRLLVPRSSDIRWIAAVSRTQYRPLLEAGVRVFEWNGPMIHAKTAVADG
RWARIGSTNLNLSSWLVNRELDIALEDEGLAGELAERFLQDLEQSTEIVLRQERRKPVPRPVSKQERRMRGTPKEMALSG
ASAAARQAMRIGDALGSVVRGSRMVESSEAPAFLSIGVALCLLALAIAYFPWLAAAPLVALLAAGGLAVVFKACGLYLEK
RKKKQQSRLAECSSGSDKPTPDDSQ
>Mature_504_residues
SKDEQSQQTLQFVRQLAEQALSRSAGAPLMAGNRVRLLYDSVENFPAWENALSECRDSIFIEMYIFANDQFGLKIRNILI
EKAKQGIQVCLLYDWLGSWRAHLSGFLKPLQEAGAEVRAYHPPSLGGGLTLLGRNHRKMIIVDRQELFVSGLCISQSWQG
QPEKGLAPWRDTGLALSGPLLAPALAAFADSWASCGEPLDARWLVPADTQPAGEAAARLIATTPSTARMMRLDLLVASFA
RRTLWLTDAYFMATSLYLSALKQAARDGVDVRLLVPRSSDIRWIAAVSRTQYRPLLEAGVRVFEWNGPMIHAKTAVADGR
WARIGSTNLNLSSWLVNRELDIALEDEGLAGELAERFLQDLEQSTEIVLRQERRKPVPRPVSKQERRMRGTPKEMALSGA
SAAARQAMRIGDALGSVVRGSRMVESSEAPAFLSIGVALCLLALAIAYFPWLAAAPLVALLAAGGLAVVFKACGLYLEKR
KKKQQSRLAECSSGSDKPTPDDSQ

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=334, Percent_Identity=29.6407185628743, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787502, Length=343, Percent_Identity=25.6559766763848, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.5

Molecular weight: Translated: 55444; Mature: 55312

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDEQSQQTLQFVRQLAEQALSRSAGAPLMAGNRVRLLYDSVENFPAWENALSECRDSI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCE
FIEMYIFANDQFGLKIRNILIEKAKQGIQVCLLYDWLGSWRAHLSGFLKPLQEAGAEVRA
EEEEEEEECCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YHPPSLGGGLTLLGRNHRKMIIVDRQELFVSGLCISQSWQGQPEKGLAPWRDTGLALSGP
ECCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEECCH
LLAPALAAFADSWASCGEPLDARWLVPADTQPAGEAAARLIATTPSTARMMRLDLLVASF
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ARRTLWLTDAYFMATSLYLSALKQAARDGVDVRLLVPRSSDIRWIAAVSRTQYRPLLEAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEHHHHHHCHHHHHCC
VRVFEWNGPMIHAKTAVADGRWARIGSTNLNLSSWLVNRELDIALEDEGLAGELAERFLQ
CEEEECCCCEEEEEEEECCCCEEEECCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHH
DLEQSTEIVLRQERRKPVPRPVSKQERRMRGTPKEMALSGASAAARQAMRIGDALGSVVR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
GSRMVESSEAPAFLSIGVALCLLALAIAYFPWLAAAPLVALLAAGGLAVVFKACGLYLEK
CCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RKKKQQSRLAECSSGSDKPTPDDSQ
HHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKDEQSQQTLQFVRQLAEQALSRSAGAPLMAGNRVRLLYDSVENFPAWENALSECRDSI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCE
FIEMYIFANDQFGLKIRNILIEKAKQGIQVCLLYDWLGSWRAHLSGFLKPLQEAGAEVRA
EEEEEEEECCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YHPPSLGGGLTLLGRNHRKMIIVDRQELFVSGLCISQSWQGQPEKGLAPWRDTGLALSGP
ECCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEECCH
LLAPALAAFADSWASCGEPLDARWLVPADTQPAGEAAARLIATTPSTARMMRLDLLVASF
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ARRTLWLTDAYFMATSLYLSALKQAARDGVDVRLLVPRSSDIRWIAAVSRTQYRPLLEAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEHHHHHHCHHHHHCC
VRVFEWNGPMIHAKTAVADGRWARIGSTNLNLSSWLVNRELDIALEDEGLAGELAERFLQ
CEEEECCCCEEEEEEEECCCCEEEECCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHH
DLEQSTEIVLRQERRKPVPRPVSKQERRMRGTPKEMALSGASAAARQAMRIGDALGSVVR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
GSRMVESSEAPAFLSIGVALCLLALAIAYFPWLAAAPLVALLAAGGLAVVFKACGLYLEK
CCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RKKKQQSRLAECSSGSDKPTPDDSQ
HHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CDPdiacylglycerol; sn-glycerol 3-phosphate

Specific reaction: CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]