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The map label for this gene is neuB

Identifier: 34499486

GI number: 34499486

Start: 4357709

End: 4358794

Strand: Reverse

Name: neuB

Synonym: CV_4031

Alternate gene names: NA

Gene position: 4358794-4357709 (Counterclockwise)

Preceding gene: 34499487

Following gene: 34499485

Centisome position: 91.74

GC content: 56.81

Gene sequence:

>1086_bases
TTGAACCACGTATTTATTATTGCTGAAGCAGGCGTGAACCATAATGGTTCTTTGAAAGTGGCCCTTGAGTTGATCGATGC
GGCCAAGGAAGCTGGCGCCGACGCGGTGAAATTCCAGACTTTCAAATCCGGCGCCGTGATCAGCATGGATGCTCCGAAAG
CCGAGTATCAAAAGACGACGACCGGTACCAGCGAAAGCCAGCTAGATATGGTTCGCAAGCTCGAATTGTCGGCTGAGGAT
CATATCCAATTGCTGCAGCATTGTGAAAAGCGCGGCATTTTGTTCCTGTCTACCCCATTCGACGTGCAAAGCGCCGAATT
GCTGGTTCGCGAACTGGGCGTGCCGCAATTGAAGGTGCCGTCCGGAGAACTGACCAATGCGCCGTTCCTGCTGAAAATCG
CGGGCTACGGCTTGCCCGTCATCGTCTCTACCGGTATGAGCACGCTGGGCGAGGCTGAAATGGCTTTGGGCGTGCTGGCC
TTCGGTTTCCTCGGACTGAGCGAAACGCCGTCCGAGACTGCATTCCGCAATGCCTTCGCGAGCGATGCGGGCCAAGCTGT
CCTGAGGGAAAAGGTCACGATCCTGCAGTGCACGACGGAATATCCGGCTCCGTTTGCTCAAGTGAATCTTAAAGCGATGG
ATACGCTCGGCGCCGCTTTCGGACTTCCTGTCGGCTTGTCGGATCATACGCCTGGCTATGCGATTCCCATCGCCGCGGTC
GCGCGAGGAGCGACTCTGATTGAAAAGCACTTCACCCTGGACCGCAATTTGCCGGGTCCGGATCACATGGCTTCTCTTGA
GCCGCATGAACTCAAAGCCATGGTGGAAGGCATCCGCCAGGTAACGGTTGCGATCGGCGACGGCTGCAAGCGTCCAAGCG
CTGACGAGATCGGCAATAGAACGATCGCGCGGAGAAGCCTGGTCGCGGCTACCGCGGTGAAGAGAGGCGAGATTTGGACG
GAGAGCAACCTGACATGCAAACGGCCAGGCGATGGCGTGTCGCCGTATCGTTTCTGGGATTTCATCGGTAAACCAGCCAC
CAGGGATTATCGCGCCGACGAAAAACTGGACGCCGGATTGGAGTAA

Upstream 100 bases:

>100_bases
CCGCCCGTATTTTATCTATACCGCTTGATCAGCGTTATGCACCGGCAGATATGCGCAAGGTGGCGAGCATGATCGGCGAA
TTTATTGCTGAGGAATTGAA

Downstream 100 bases:

>100_bases
TCATGGCGCATGATTGGGAAAAGGTACTGCTGTCTCCTGATACGCCTGCTGAATCAGCGTTGAGAGTGCTGGATGACAGT
GGCTTGCGATTGGTGCTGGT

Product: N-acetylneuraminic acid synthase

Products: phosphate; N-acetylneuraminate

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNHVFIIAEAGVNHNGSLKVALELIDAAKEAGADAVKFQTFKSGAVISMDAPKAEYQKTTTGTSESQLDMVRKLELSAED
HIQLLQHCEKRGILFLSTPFDVQSAELLVRELGVPQLKVPSGELTNAPFLLKIAGYGLPVIVSTGMSTLGEAEMALGVLA
FGFLGLSETPSETAFRNAFASDAGQAVLREKVTILQCTTEYPAPFAQVNLKAMDTLGAAFGLPVGLSDHTPGYAIPIAAV
ARGATLIEKHFTLDRNLPGPDHMASLEPHELKAMVEGIRQVTVAIGDGCKRPSADEIGNRTIARRSLVAATAVKRGEIWT
ESNLTCKRPGDGVSPYRFWDFIGKPATRDYRADEKLDAGLE

Sequences:

>Translated_361_residues
MNHVFIIAEAGVNHNGSLKVALELIDAAKEAGADAVKFQTFKSGAVISMDAPKAEYQKTTTGTSESQLDMVRKLELSAED
HIQLLQHCEKRGILFLSTPFDVQSAELLVRELGVPQLKVPSGELTNAPFLLKIAGYGLPVIVSTGMSTLGEAEMALGVLA
FGFLGLSETPSETAFRNAFASDAGQAVLREKVTILQCTTEYPAPFAQVNLKAMDTLGAAFGLPVGLSDHTPGYAIPIAAV
ARGATLIEKHFTLDRNLPGPDHMASLEPHELKAMVEGIRQVTVAIGDGCKRPSADEIGNRTIARRSLVAATAVKRGEIWT
ESNLTCKRPGDGVSPYRFWDFIGKPATRDYRADEKLDAGLE
>Mature_361_residues
MNHVFIIAEAGVNHNGSLKVALELIDAAKEAGADAVKFQTFKSGAVISMDAPKAEYQKTTTGTSESQLDMVRKLELSAED
HIQLLQHCEKRGILFLSTPFDVQSAELLVRELGVPQLKVPSGELTNAPFLLKIAGYGLPVIVSTGMSTLGEAEMALGVLA
FGFLGLSETPSETAFRNAFASDAGQAVLREKVTILQCTTEYPAPFAQVNLKAMDTLGAAFGLPVGLSDHTPGYAIPIAAV
ARGATLIEKHFTLDRNLPGPDHMASLEPHELKAMVEGIRQVTVAIGDGCKRPSADEIGNRTIARRSLVAATAVKRGEIWT
ESNLTCKRPGDGVSPYRFWDFIGKPATRDYRADEKLDAGLE

Specific function: Unknown

COG id: COG2089

COG function: function code M; Sialic acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis spsE [H]

Homologues:

Organism=Homo sapiens, GI12056473, Length=345, Percent_Identity=33.6231884057971, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21357081, Length=385, Percent_Identity=28.3116883116883, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006190
- InterPro:   IPR013785
- InterPro:   IPR013132
- InterPro:   IPR013974 [H]

Pfam domain/function: PF03102 NeuB; PF08666 SAF [H]

EC number: 2.5.1.56

Molecular weight: Translated: 38718; Mature: 38718

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50844 AFP_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHVFIIAEAGVNHNGSLKVALELIDAAKEAGADAVKFQTFKSGAVISMDAPKAEYQKTT
CCEEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCHHHHHCC
TGTSESQLDMVRKLELSAEDHIQLLQHCEKRGILFLSTPFDVQSAELLVRELGVPQLKVP
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEECC
SGELTNAPFLLKIAGYGLPVIVSTGMSTLGEAEMALGVLAFGFLGLSETPSETAFRNAFA
CCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SDAGQAVLREKVTILQCTTEYPAPFAQVNLKAMDTLGAAFGLPVGLSDHTPGYAIPIAAV
HHHHHHHHHHCEEEEEEECCCCCCHHHEEHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHH
ARGATLIEKHFTLDRNLPGPDHMASLEPHELKAMVEGIRQVTVAIGDGCKRPSADEIGNR
HCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCH
TIARRSLVAATAVKRGEIWTESNLTCKRPGDGVSPYRFWDFIGKPATRDYRADEKLDAGL
HHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
E
C
>Mature Secondary Structure
MNHVFIIAEAGVNHNGSLKVALELIDAAKEAGADAVKFQTFKSGAVISMDAPKAEYQKTT
CCEEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCHHHHHCC
TGTSESQLDMVRKLELSAEDHIQLLQHCEKRGILFLSTPFDVQSAELLVRELGVPQLKVP
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEECC
SGELTNAPFLLKIAGYGLPVIVSTGMSTLGEAEMALGVLAFGFLGLSETPSETAFRNAFA
CCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SDAGQAVLREKVTILQCTTEYPAPFAQVNLKAMDTLGAAFGLPVGLSDHTPGYAIPIAAV
HHHHHHHHHHCEEEEEEECCCCCCHHHEEHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHH
ARGATLIEKHFTLDRNLPGPDHMASLEPHELKAMVEGIRQVTVAIGDGCKRPSADEIGNR
HCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCH
TIARRSLVAATAVKRGEIWTESNLTCKRPGDGVSPYRFWDFIGKPATRDYRADEKLDAGL
HHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; N-acetyl-D-mannosamine; H2O

Specific reaction: phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O = phosphate + N-acetylneuraminate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]