Click here to switch to the map view.

The map label for this gene is wcaI [H]

Identifier: 34499476

GI number: 34499476

Start: 4347018

End: 4348244

Strand: Reverse

Name: wcaI [H]

Synonym: CV_4021

Alternate gene names: 34499476

Gene position: 4348244-4347018 (Counterclockwise)

Preceding gene: 34499477

Following gene: 34499475

Centisome position: 91.52

GC content: 42.22

Gene sequence:

>1227_bases
GTGCATATTTTATTTTTAACTGATAATTTTCCGCCGGAAGTAAATGCTCCCGCAAGTAGAACATTTGAACATTGCCGCGA
GTGGGTTAGAAATGGCGCTCAGGTAACTATTATTACTTGCGCTCCAAATTTCCCGAAAGGTGAGGTCTTTCCTGGTTATA
AGAATTCCTTGTGGTCTACGGAGCTTGTCGACGGAATCCGCGTTGTTCGAGTGTGGACATATATTGCAAAAAATGAAGGT
CTCCTGAAGCGTATTTTTGATTATATCAGCTTTATGGTAAGTGCCTCTATTGCTGCTTTGTTTGTGAAAAATGTTGATTT
GATTATTGGCACGTCGCCACAGTTTTTTACTGTGTGTGCTGCTGCTATTACATCTCGATTTAAACGTGTGCCATGGATTT
TTGAGTTGAGGGATATTTGGCCTGAGTCGATTAAGGCTGTAGGCGCTATGGGCGACTCATTCCTGATACGTCAATTGGAG
AAGCTAGAGTTGTTCTTATATCGCGACTGTAAAAAAATAGTTGTGGTAACGCATGCCTTTAAAGATAGCCTGATTAGACG
GGGTATCAATTCTGATAAGATTGATGTTGTAACCAATGGTGTAGATCTTTCCAATTACTCTCCGCGAGAGAAAGATTCAG
AGTTGGTCGATAGTCTTGGCTTGCAGGGGTGTTTTGTTGCTGGCTATATCGGTACACATGGCATGGCTCATGGACTAGAG
ACGCTGCTGAACGCCGCGGAGAGGCTGAAATCGCTTCCAGAGGGAAGGGATGTGAAATTCCTTTTCCTTGGCGATGGCGC
TAGAAAAAAGGACTTGGTCATGGATGCCAAGTCTAGAAATTTAGATAATGTATTGTTTTTAGATAGCGTCTCTAAAGAGC
AGGTATCGCGTTATTGGTCACTATTAGATGTATCTATTATTCATCTAAAAAAGACAGATTTGTTTAATAGCGTGATTCCG
TCTAAGTTGTTTGAATGCATGGGCATGGGGATTCCTGTTCTGCATGGGGTGCCTGGAGAGTCGGCAGAAATTGTGCGAGG
CGAGGAGGTGGGCATAACTTTTGAATCTGATGATGATGAAGCTTTGGCTGATGCTTTGCTGGTGATGAAGTCTGATACTG
AGGCATACCAAGGATTTCGTAGTAGGTGTTTAGAAGGTGCGCTGAAATATAATAGACGTAATCTGGCATTGAAAATGCTT
GATATTTTAAAGAAGGTGAATGTCTAA

Upstream 100 bases:

>100_bases
CATCCCAAGGCTTTGTCTCGGGCCTCGGTTGAGGTTTTGGCTGATTTTGCCAAAGCAGAGAGAAATCGTTTCTCGATAAT
GTAATATCGGATATAGCGCT

Downstream 100 bases:

>100_bases
GATGAGTACGCGTAAGAAAATTATGCTAGCTTTTGGCACGAGGCCAGAGGCGATTAAAATGGCTCCTCTTTATCATGCTT
TGAAAAGCTGCGAAGAAAAT

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MHILFLTDNFPPEVNAPASRTFEHCREWVRNGAQVTIITCAPNFPKGEVFPGYKNSLWSTELVDGIRVVRVWTYIAKNEG
LLKRIFDYISFMVSASIAALFVKNVDLIIGTSPQFFTVCAAAITSRFKRVPWIFELRDIWPESIKAVGAMGDSFLIRQLE
KLELFLYRDCKKIVVVTHAFKDSLIRRGINSDKIDVVTNGVDLSNYSPREKDSELVDSLGLQGCFVAGYIGTHGMAHGLE
TLLNAAERLKSLPEGRDVKFLFLGDGARKKDLVMDAKSRNLDNVLFLDSVSKEQVSRYWSLLDVSIIHLKKTDLFNSVIP
SKLFECMGMGIPVLHGVPGESAEIVRGEEVGITFESDDDEALADALLVMKSDTEAYQGFRSRCLEGALKYNRRNLALKML
DILKKVNV

Sequences:

>Translated_408_residues
MHILFLTDNFPPEVNAPASRTFEHCREWVRNGAQVTIITCAPNFPKGEVFPGYKNSLWSTELVDGIRVVRVWTYIAKNEG
LLKRIFDYISFMVSASIAALFVKNVDLIIGTSPQFFTVCAAAITSRFKRVPWIFELRDIWPESIKAVGAMGDSFLIRQLE
KLELFLYRDCKKIVVVTHAFKDSLIRRGINSDKIDVVTNGVDLSNYSPREKDSELVDSLGLQGCFVAGYIGTHGMAHGLE
TLLNAAERLKSLPEGRDVKFLFLGDGARKKDLVMDAKSRNLDNVLFLDSVSKEQVSRYWSLLDVSIIHLKKTDLFNSVIP
SKLFECMGMGIPVLHGVPGESAEIVRGEEVGITFESDDDEALADALLVMKSDTEAYQGFRSRCLEGALKYNRRNLALKML
DILKKVNV
>Mature_408_residues
MHILFLTDNFPPEVNAPASRTFEHCREWVRNGAQVTIITCAPNFPKGEVFPGYKNSLWSTELVDGIRVVRVWTYIAKNEG
LLKRIFDYISFMVSASIAALFVKNVDLIIGTSPQFFTVCAAAITSRFKRVPWIFELRDIWPESIKAVGAMGDSFLIRQLE
KLELFLYRDCKKIVVVTHAFKDSLIRRGINSDKIDVVTNGVDLSNYSPREKDSELVDSLGLQGCFVAGYIGTHGMAHGLE
TLLNAAERLKSLPEGRDVKFLFLGDGARKKDLVMDAKSRNLDNVLFLDSVSKEQVSRYWSLLDVSIIHLKKTDLFNSVIP
SKLFECMGMGIPVLHGVPGESAEIVRGEEVGITFESDDDEALADALLVMKSDTEAYQGFRSRCLEGALKYNRRNLALKML
DILKKVNV

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=405, Percent_Identity=23.2098765432099, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 45784; Mature: 45784

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILFLTDNFPPEVNAPASRTFEHCREWVRNGAQVTIITCAPNFPKGEVFPGYKNSLWST
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHH
ELVDGIRVVRVWTYIAKNEGLLKRIFDYISFMVSASIAALFVKNVDLIIGTSPQFFTVCA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
AAITSRFKRVPWIFELRDIWPESIKAVGAMGDSFLIRQLEKLELFLYRDCKKIVVVTHAF
HHHHHHHHHCCEEEEHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHH
KDSLIRRGINSDKIDVVTNGVDLSNYSPREKDSELVDSLGLQGCFVAGYIGTHGMAHGLE
HHHHHHCCCCCCCEEEEECCEECCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
TLLNAAERLKSLPEGRDVKFLFLGDGARKKDLVMDAKSRNLDNVLFLDSVSKEQVSRYWS
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHEECCCCCCCCCEEEECCCCHHHHHHHHH
LLDVSIIHLKKTDLFNSVIPSKLFECMGMGIPVLHGVPGESAEIVRGEEVGITFESDDDE
HHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEECCCEEEEEEECCCHH
ALADALLVMKSDTEAYQGFRSRCLEGALKYNRRNLALKMLDILKKVNV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHILFLTDNFPPEVNAPASRTFEHCREWVRNGAQVTIITCAPNFPKGEVFPGYKNSLWST
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHH
ELVDGIRVVRVWTYIAKNEGLLKRIFDYISFMVSASIAALFVKNVDLIIGTSPQFFTVCA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
AAITSRFKRVPWIFELRDIWPESIKAVGAMGDSFLIRQLEKLELFLYRDCKKIVVVTHAF
HHHHHHHHHCCEEEEHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHH
KDSLIRRGINSDKIDVVTNGVDLSNYSPREKDSELVDSLGLQGCFVAGYIGTHGMAHGLE
HHHHHHCCCCCCCEEEEECCEECCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
TLLNAAERLKSLPEGRDVKFLFLGDGARKKDLVMDAKSRNLDNVLFLDSVSKEQVSRYWS
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHEECCCCCCCCCEEEECCCCHHHHHHHHH
LLDVSIIHLKKTDLFNSVIPSKLFECMGMGIPVLHGVPGESAEIVRGEEVGITFESDDDE
HHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEECCCEEEEEEECCCHH
ALADALLVMKSDTEAYQGFRSRCLEGALKYNRRNLALKMLDILKKVNV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]