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The map label for this gene is intZ [H]

Identifier: 34499306

GI number: 34499306

Start: 4155884

End: 4157155

Strand: Direct

Name: intZ [H]

Synonym: CV_3851

Alternate gene names: 34499306

Gene position: 4155884-4157155 (Clockwise)

Preceding gene: 34499305

Following gene: 34499307

Centisome position: 87.47

GC content: 64.78

Gene sequence:

>1272_bases
ATGGGCAAGCTGACCGACATGCAGATCAAGGCCTGGATCAAGGCCGGGGAACGATTCGAGGGCCGCGGCGACGGTGATGG
GCTCTGGCTGCGCTTCCGCGAGAATGACAAGGTGCCGGTCTGGCGCTACCGCTACAAGTTCGCCGGCAAGTCCCGCACCA
TGCAGATCGGCAGCTACGCAGAGTTGTCGCTTGCCAAAGCCAGAGAAGCCGTCAAATCCCTCTCCGCCCGCGTGGCGCTG
GGCCATGATGTTGCCGCGGAGAAGCAGGAGAAAAAGTCCGCAGCCCTGGCCAAGATCGAGGAAGAGAAGAACGCCGTCAC
CATGTTGGCGCTGGCGAATGAGTACTACGAGCGGATGATCCTGGGCCGCTGGAAGCACCCGGACATCGTGCGGCGCCGGA
TCGACAAAGACATCGGCCCCATGCTGGGCAAGATGAAGGTAGAGGACATCAAGCCCCGCCACATCGACGACATGCTGCAG
ACCATCGTCAAGCGCGGCGCGCCTACCATCGCCAACGACGTGCTGCGCTGGACCCGCCGCATGTTCGACTACGCCATCAA
GCGCCACATCATCGAGATCAACCCGGCCAGCGCCTTCGATCTGGGCGACGCCGGTGGCAAGGAAGAGGCCCGCGAGCGCG
CGCTGAGCCGCGACGAGCTGGCCAAGCTGTTCCAGGCCATGAAGCTAGCCCGCGGCTTCAGCGTGGAAAACGACCTCGCC
ATCCGCCTGCTGCTGTTGCTCTGCGTCCGCAAGATGGAGCTGTGCGCCGCGCGCAAGGAAGAGTTCGACTTGCCGGCCGG
CGTCTGGCACCTGCCAGGCGAGCGCAGCAAGACCGGCAAGCCTATCGACATCCCCTTGCCCGCCTTGGCGGTGGAATGGC
TGCAGCAGCTGTTCGACCTGGCCGGCCCCAGCGCCTGGCTGCTGCCGGCCCGCAAGATGCAGCACCGCATGGTGCCGCAC
ATCCACGAGGGCACCATCGGCACCGCACTGGGCAAGGTTAAGCCGCACATGCCGGACGTGGCGGCCTTCACCATCCACGA
CCTGCGCCGCACCGCGCGCACCCACCTGGCGGAATTGGGCGTCGACCCGGTGGTGGCCGAGCGCTGCCTGAATCACAAGA
TCAAGGGCGTGGAAGGCATCTACAACCGTCACGACTACTTCAACGAGCGCCGCGACGCCCTGAACAAGTGGGCCGGGCTG
CTTGATGCCCTGGACAAGGGAGAGGCCTACAACGTGACGCCAATTACCAAGGGTCGGATCAAGACGGCCTGA

Upstream 100 bases:

>100_bases
AGGGGCGTTTGGGTACACCCCCTCTATTCGCTGTTCAGATTTTTCCCGATCCAGCAGCGGTGCGGGTTTAAGGGGGCTAC
TCAGTATTCGAGGTGCAAAC

Downstream 100 bases:

>100_bases
CACCAGCTTTGCCACGCCTAGATCGGCCAATCGAAAAGCGGGAAACCCTGCCCGCCTGGCGCGGCAACCCATTCTCAGGG
TTGCGCAAAGGGAGCGCATG

Product: prophage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MGKLTDMQIKAWIKAGERFEGRGDGDGLWLRFRENDKVPVWRYRYKFAGKSRTMQIGSYAELSLAKAREAVKSLSARVAL
GHDVAAEKQEKKSAALAKIEEEKNAVTMLALANEYYERMILGRWKHPDIVRRRIDKDIGPMLGKMKVEDIKPRHIDDMLQ
TIVKRGAPTIANDVLRWTRRMFDYAIKRHIIEINPASAFDLGDAGGKEEARERALSRDELAKLFQAMKLARGFSVENDLA
IRLLLLLCVRKMELCAARKEEFDLPAGVWHLPGERSKTGKPIDIPLPALAVEWLQQLFDLAGPSAWLLPARKMQHRMVPH
IHEGTIGTALGKVKPHMPDVAAFTIHDLRRTARTHLAELGVDPVVAERCLNHKIKGVEGIYNRHDYFNERRDALNKWAGL
LDALDKGEAYNVTPITKGRIKTA

Sequences:

>Translated_423_residues
MGKLTDMQIKAWIKAGERFEGRGDGDGLWLRFRENDKVPVWRYRYKFAGKSRTMQIGSYAELSLAKAREAVKSLSARVAL
GHDVAAEKQEKKSAALAKIEEEKNAVTMLALANEYYERMILGRWKHPDIVRRRIDKDIGPMLGKMKVEDIKPRHIDDMLQ
TIVKRGAPTIANDVLRWTRRMFDYAIKRHIIEINPASAFDLGDAGGKEEARERALSRDELAKLFQAMKLARGFSVENDLA
IRLLLLLCVRKMELCAARKEEFDLPAGVWHLPGERSKTGKPIDIPLPALAVEWLQQLFDLAGPSAWLLPARKMQHRMVPH
IHEGTIGTALGKVKPHMPDVAAFTIHDLRRTARTHLAELGVDPVVAERCLNHKIKGVEGIYNRHDYFNERRDALNKWAGL
LDALDKGEAYNVTPITKGRIKTA
>Mature_422_residues
GKLTDMQIKAWIKAGERFEGRGDGDGLWLRFRENDKVPVWRYRYKFAGKSRTMQIGSYAELSLAKAREAVKSLSARVALG
HDVAAEKQEKKSAALAKIEEEKNAVTMLALANEYYERMILGRWKHPDIVRRRIDKDIGPMLGKMKVEDIKPRHIDDMLQT
IVKRGAPTIANDVLRWTRRMFDYAIKRHIIEINPASAFDLGDAGGKEEARERALSRDELAKLFQAMKLARGFSVENDLAI
RLLLLLCVRKMELCAARKEEFDLPAGVWHLPGERSKTGKPIDIPLPALAVEWLQQLFDLAGPSAWLLPARKMQHRMVPHI
HEGTIGTALGKVKPHMPDVAAFTIHDLRRTARTHLAELGVDPVVAERCLNHKIKGVEGIYNRHDYFNERRDALNKWAGLL
DALDKGEAYNVTPITKGRIKTA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=391, Percent_Identity=27.6214833759591, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788974, Length=409, Percent_Identity=27.3838630806846, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788690, Length=387, Percent_Identity=25.3229974160207, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47969; Mature: 47838

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLTDMQIKAWIKAGERFEGRGDGDGLWLRFRENDKVPVWRYRYKFAGKSRTMQIGSYA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEECCCHH
ELSLAKAREAVKSLSARVALGHDVAAEKQEKKSAALAKIEEEKNAVTMLALANEYYERMI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LGRWKHPDIVRRRIDKDIGPMLGKMKVEDIKPRHIDDMLQTIVKRGAPTIANDVLRWTRR
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
MFDYAIKRHIIEINPASAFDLGDAGGKEEARERALSRDELAKLFQAMKLARGFSVENDLA
HHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHH
IRLLLLLCVRKMELCAARKEEFDLPAGVWHLPGERSKTGKPIDIPLPALAVEWLQQLFDL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGPSAWLLPARKMQHRMVPHIHEGTIGTALGKVKPHMPDVAAFTIHDLRRTARTHLAELG
CCCCEEECCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
VDPVVAERCLNHKIKGVEGIYNRHDYFNERRDALNKWAGLLDALDKGEAYNVTPITKGRI
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
KTA
CCC
>Mature Secondary Structure 
GKLTDMQIKAWIKAGERFEGRGDGDGLWLRFRENDKVPVWRYRYKFAGKSRTMQIGSYA
CCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEECCCHH
ELSLAKAREAVKSLSARVALGHDVAAEKQEKKSAALAKIEEEKNAVTMLALANEYYERMI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LGRWKHPDIVRRRIDKDIGPMLGKMKVEDIKPRHIDDMLQTIVKRGAPTIANDVLRWTRR
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
MFDYAIKRHIIEINPASAFDLGDAGGKEEARERALSRDELAKLFQAMKLARGFSVENDLA
HHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHH
IRLLLLLCVRKMELCAARKEEFDLPAGVWHLPGERSKTGKPIDIPLPALAVEWLQQLFDL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGPSAWLLPARKMQHRMVPHIHEGTIGTALGKVKPHMPDVAAFTIHDLRRTARTHLAELG
CCCCEEECCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
VDPVVAERCLNHKIKGVEGIYNRHDYFNERRDALNKWAGLLDALDKGEAYNVTPITKGRI
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
KTA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]