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The map label for this gene is gspI [H]

Identifier: 34499264

GI number: 34499264

Start: 4107682

End: 4108062

Strand: Reverse

Name: gspI [H]

Synonym: CV_3809

Alternate gene names: 34499264

Gene position: 4108062-4107682 (Counterclockwise)

Preceding gene: 34499265

Following gene: 34499263

Centisome position: 86.47

GC content: 69.03

Gene sequence:

>381_bases
ATGAAGCGGCAGCAAGGCTTCACCCTGTTCGAGGTATTGGTCGCGCTGGCCATCATCGCCGTGGCGCTGGGCGCCCTGCT
GCGCGCCACCGGCCTCGCCGCCGACAACGCCGAGGGCATGGAGCGGCGCATGCAGGCCAATTGGGAGGCGCAGAACCAGA
TCGCGATGCTGCAGGCGCTGCGGCAATTCCCGGAGCCGGGACAGCAGGCCGGCGAAAGCAAGGAAGACGGCGTCGAGTGG
CGCTGGGAGCGTGAAATCTCCCCCACGCCCAACCCCAACTTCCGCAAGGTGGTGGTGCGCATCCTGGCGCCCGGCGCCGG
CCATTACGTGCTGGCGGAAATCACCGGCTATCTCCGGCAGCAGGCGGGAGGCGGGGGGTGA

Upstream 100 bases:

>100_bases
ACGGCCGCGCCGCCGCGCTGGCGCTCAGGCTGGACGGCGCCGGCCGCAAGCTGACGGTTTTGCAGTCGCCGCTGGGCCGG
GTGACGGTGGCGGACGGATC

Downstream 100 bases:

>100_bases
GCGCGCGGCGGCAGGGCGGCATGACGCTGCTGGAAATCCTGGTGGCGATGAGCCTGTTGGCCATCCTGTCGGTGATGGGC
TACAAGGCTTTCGGCTCGCT

Product: general secretion pathway protein I

Products: NA

Alternate protein names: PilD-dependent protein pddC [H]

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MKRQQGFTLFEVLVALAIIAVALGALLRATGLAADNAEGMERRMQANWEAQNQIAMLQALRQFPEPGQQAGESKEDGVEW
RWEREISPTPNPNFRKVVVRILAPGAGHYVLAEITGYLRQQAGGGG

Sequences:

>Translated_126_residues
MKRQQGFTLFEVLVALAIIAVALGALLRATGLAADNAEGMERRMQANWEAQNQIAMLQALRQFPEPGQQAGESKEDGVEW
RWEREISPTPNPNFRKVVVRILAPGAGHYVLAEITGYLRQQAGGGG
>Mature_126_residues
MKRQQGFTLFEVLVALAIIAVALGALLRATGLAADNAEGMERRMQANWEAQNQIAMLQALRQFPEPGQQAGESKEDGVEW
RWEREISPTPNPNFRKVVVRILAPGAGHYVLAEITGYLRQQAGGGG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP I family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010052
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR003413 [H]

Pfam domain/function: PF02501 GSPII_IJ; PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 13800; Mature: 13800

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQQGFTLFEVLVALAIIAVALGALLRATGLAADNAEGMERRMQANWEAQNQIAMLQAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
RQFPEPGQQAGESKEDGVEWRWEREISPTPNPNFRKVVVRILAPGAGHYVLAEITGYLRQ
HHCCCCCHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
QAGGGG
HCCCCC
>Mature Secondary Structure
MKRQQGFTLFEVLVALAIIAVALGALLRATGLAADNAEGMERRMQANWEAQNQIAMLQAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
RQFPEPGQQAGESKEDGVEWRWEREISPTPNPNFRKVVVRILAPGAGHYVLAEITGYLRQ
HHCCCCCHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
QAGGGG
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1588814; 1309616; 10984043 [H]