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The map label for this gene is dadB [H]

Identifier: 34499248

GI number: 34499248

Start: 4088183

End: 4089256

Strand: Direct

Name: dadB [H]

Synonym: CV_3793

Alternate gene names: 34499248

Gene position: 4088183-4089256 (Clockwise)

Preceding gene: 34499246

Following gene: 34499254

Centisome position: 86.05

GC content: 68.34

Gene sequence:

>1074_bases
ATGACACGCCCTATCCGGCTGGAGATTTCCCTCCCGGCCATCCGCCATAATTTCCTGTTCACCCGCCAGCATTCCCAGGC
CCGCAGCCTGTTCGCCGTGATCAAGGCGAACGCCTACGGCCACGGCTTATTGGACACTGCAAAGGCGTTGGCCGATCAGG
CCGACGGTTTTGCCCTGCTCAATATCGAAGATGCCGTGCGCCTGCGCGAAGCGGGATTCGATCACCCCATCTCGCTGCTG
GAGGGACCGTTCGACGCGGCCGAAGCCGCCGCGATGGCCGATTACCGCCTGGGCGGCGCGGTCCACTCACAACAACAGAT
GGACTGGCTGGCCAAAGGTTCCGCGGATCGGCCCATCGACGTCTGGCTGAAAATCAACAGCGGCATGAACCGGCTGGGTT
TCCGCCCGCAAGAGGCCGCCGCCGCCCAAGCCCGGTTGCAGGCGCTGCCCGGCGCGCGGCTGACCGCGGTCATGACCCAC
TTCGCCACCGCCGATGAAGATTACGGCGTCGCCGAGCAATGGGCCCGCTTCGAGCCGGTGGCCGTCGCCAGCGGACTGAA
GGTCTGCGCCGCCAATTCCGCCGCACTGCTGCGCCATCCGCATACCCACGGCGACATCGGCCGCCCCGGCATCGTGCTCT
ACGGCGCCTCGCCGTTCGAAAACGAAACCGGCGCCGGCCTCGGCCTGATGCCGGCGATGACGCTGTCGGCCGACATCATC
GCCGTGCAACAGCTGCAACCCGGCGACGCGGTGGGCTACGGCCGGCGTTTCATCGCCGACCGGGCCATGCGCATCGGCGT
GGTCGCCTGCGGCTACGCCGACGGCTATCCTCGCGTCGCCGCCAACGGCGCGCCGGCGGCGGTGGACGGCCAACCCTGTC
GACTGGCGGGCCGGGTATCGATGGACATGCTGACGGTGGATATCAGCCATCTGCCCCAGGCCGGCGTCGGCAGCCGGGTG
GAGCTGTGGGGGCCAAACGCGCCGATAGAGAAAGTGGCCGCGGCCGCCGGCACCATCAGCTACGAGCTGATGAGCGCGGT
GATGCCGCGGGTACCGCGCGTCGTGCGCAGCTGA

Upstream 100 bases:

>100_bases
CCGCCGGCTCATTCCAAATCGTCGCGATCAAGCGGCTGGGCGGCTAATGTCCATGCACGGAGGTGTATAATAGCACACCT
CTCCTACACGCTTTCTTGAC

Downstream 100 bases:

>100_bases
GTCCAAGCAGCAGCGGCGGCGCCAGCAAGGCGCCGCAGACATGCGCCGACCACAGCACCGGCTCGCCGCCCGGCCCCAGA
TTCGGCCACATGCCCTGCCA

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTRPIRLEISLPAIRHNFLFTRQHSQARSLFAVIKANAYGHGLLDTAKALADQADGFALLNIEDAVRLREAGFDHPISLL
EGPFDAAEAAAMADYRLGGAVHSQQQMDWLAKGSADRPIDVWLKINSGMNRLGFRPQEAAAAQARLQALPGARLTAVMTH
FATADEDYGVAEQWARFEPVAVASGLKVCAANSAALLRHPHTHGDIGRPGIVLYGASPFENETGAGLGLMPAMTLSADII
AVQQLQPGDAVGYGRRFIADRAMRIGVVACGYADGYPRVAANGAPAAVDGQPCRLAGRVSMDMLTVDISHLPQAGVGSRV
ELWGPNAPIEKVAAAAGTISYELMSAVMPRVPRVVRS

Sequences:

>Translated_357_residues
MTRPIRLEISLPAIRHNFLFTRQHSQARSLFAVIKANAYGHGLLDTAKALADQADGFALLNIEDAVRLREAGFDHPISLL
EGPFDAAEAAAMADYRLGGAVHSQQQMDWLAKGSADRPIDVWLKINSGMNRLGFRPQEAAAAQARLQALPGARLTAVMTH
FATADEDYGVAEQWARFEPVAVASGLKVCAANSAALLRHPHTHGDIGRPGIVLYGASPFENETGAGLGLMPAMTLSADII
AVQQLQPGDAVGYGRRFIADRAMRIGVVACGYADGYPRVAANGAPAAVDGQPCRLAGRVSMDMLTVDISHLPQAGVGSRV
ELWGPNAPIEKVAAAAGTISYELMSAVMPRVPRVVRS
>Mature_356_residues
TRPIRLEISLPAIRHNFLFTRQHSQARSLFAVIKANAYGHGLLDTAKALADQADGFALLNIEDAVRLREAGFDHPISLLE
GPFDAAEAAAMADYRLGGAVHSQQQMDWLAKGSADRPIDVWLKINSGMNRLGFRPQEAAAAQARLQALPGARLTAVMTHF
ATADEDYGVAEQWARFEPVAVASGLKVCAANSAALLRHPHTHGDIGRPGIVLYGASPFENETGAGLGLMPAMTLSADIIA
VQQLQPGDAVGYGRRFIADRAMRIGVVACGYADGYPRVAANGAPAAVDGQPCRLAGRVSMDMLTVDISHLPQAGVGSRVE
LWGPNAPIEKVAAAAGTISYELMSAVMPRVPRVVRS

Specific function: Provides the D-alanine required for cell wall biosynthesis [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=356, Percent_Identity=46.9101123595506, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1790487, Length=347, Percent_Identity=41.7867435158501, Blast_Score=264, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 37932; Mature: 37801

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIRLEISLPAIRHNFLFTRQHSQARSLFAVIKANAYGHGLLDTAKALADQADGFALL
CCCCEEEEEECCHHHCCEEEEECHHHHHHEEHHEECCCCCCCHHHHHHHHHHCCCCEEEE
NIEDAVRLREAGFDHPISLLEGPFDAAEAAAMADYRLGGAVHSQQQMDWLAKGSADRPID
EHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEE
VWLKINSGMNRLGFRPQEAAAAQARLQALPGARLTAVMTHFATADEDYGVAEQWARFEPV
EEEEECCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCH
AVASGLKVCAANSAALLRHPHTHGDIGRPGIVLYGASPFENETGAGLGLMPAMTLSADII
HHHCCCEEEECCCHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCEE
AVQQLQPGDAVGYGRRFIADRAMRIGVVACGYADGYPRVAANGAPAAVDGQPCRLAGRVS
EEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCEEECCCCCCCCCCCCCEEECCCE
MDMLTVDISHLPQAGVGSRVELWGPNAPIEKVAAAAGTISYELMSAVMPRVPRVVRS
EEEEEEEHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRPIRLEISLPAIRHNFLFTRQHSQARSLFAVIKANAYGHGLLDTAKALADQADGFALL
CCCEEEEEECCHHHCCEEEEECHHHHHHEEHHEECCCCCCCHHHHHHHHHHCCCCEEEE
NIEDAVRLREAGFDHPISLLEGPFDAAEAAAMADYRLGGAVHSQQQMDWLAKGSADRPID
EHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEE
VWLKINSGMNRLGFRPQEAAAAQARLQALPGARLTAVMTHFATADEDYGVAEQWARFEPV
EEEEECCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCH
AVASGLKVCAANSAALLRHPHTHGDIGRPGIVLYGASPFENETGAGLGLMPAMTLSADII
HHHCCCEEEECCCHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCEE
AVQQLQPGDAVGYGRRFIADRAMRIGVVACGYADGYPRVAANGAPAAVDGQPCRLAGRVS
EEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCEEECCCCCCCCCCCCCEEECCCE
MDMLTVDISHLPQAGVGSRVELWGPNAPIEKVAAAAGTISYELMSAVMPRVPRVVRS
EEEEEEEHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA