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The map label for this gene is yiaE [C]

Identifier: 34499244

GI number: 34499244

Start: 4085179

End: 4086129

Strand: Reverse

Name: yiaE [C]

Synonym: CV_3789

Alternate gene names: 34499244

Gene position: 4086129-4085179 (Counterclockwise)

Preceding gene: 34499245

Following gene: 34499242

Centisome position: 86.0

GC content: 66.35

Gene sequence:

>951_bases
ATGACTCATAACGTCGTATTTCTGGACCGGGACAGCTTGCCTGTCCCGGTTCCCGTTTTTGGCTTCCCGGTTCATTACCG
CGAATATCCGGCGACCGCCTCCGAGCAGCTCGCAGCCCATGCGGCCGGCGCCGACATCGTGATCAGCAACAAGGTGCCGC
TGTCCCGCGAGGCCATCGCCGCACTGCCCGAACTCAGGCTGGTCGCCATCGCCGCCACCGGCTACAACCACGTCGATCTG
GATGCCTGCCGCGAACGCGGCGTCGCGGTATGCAATATCCGCCATTACGGCGATCACACGGTGGCCGAGCACGCGTTTAT
GCTGATGATGGCGCTGATGAAGAATCTGCCCGCCTACCAGCGCGACGTCGCCGCCGGCGTGTGGAGCCAGTCGCGGCAGT
TCTGCCATTTCGGCGCGCCGATACGCGAAGTGCGCGGCGCGACGCTGGCCATTGTCGGCAGCGGGGGGATAGGCGGACGG
CTGGCGCAGATGGCGCGCGCCTTCGGCATGGAGGTGCTGTTCGCCGAACGCAAGGGCGCGACCCGGGTCAGGGAGGAGCG
GGTGCCGTTCGACGAGGCGCTGGCCCGGGCCGACGTGGTGTCGTTGCATTGCCCGCTGACCGAGGAGACCCGCGGCATGA
TCGCCCAGCCTGAGCTGATGGCGATGAAGCCAGGAGCCATCCTGATCAATACCGCGCGCGGAGGCCTGGTGGATGAGGCC
GATCTGGTGGCGGCGCTGAAATACGGACAGCTGGGCGGGGCCGGTTTCGACGTGCTGAGCAGCGAGCCGCCGTCGCCGGA
TAACCCTCTGCTTAAGGCGCGCCTGCCCAACCTGATCGTCACGCCGCACGTGGGCTGGGCCAGCGGCGAAGCGATGCGCC
GGCTCGCCGCGCAGCTGGTCGCCAACATCGAGGCGTTCGTCGATGGACGGAAAGTAAACCGTTTAATTTAA

Upstream 100 bases:

>100_bases
ACCTATTACCTGGGCCTGATCATCATCATGCCGGTGCTGGCGCTGGCCAGCTGGCATGCTTATCGGGATCTGATCACCTT
CGAGCGATGACCGCCACGCA

Downstream 100 bases:

>100_bases
TCAGAAATTAGTGTTTTTGTGGTGATGCCTGTCATCCTTTGCAGGAAAACAAGCCGCCGCTGTCATGTTGGCTATGATTT
TATTGTTTTTATTGAAAAAG

Product: glycerate dehydrogenase

Products: hydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTHNVVFLDRDSLPVPVPVFGFPVHYREYPATASEQLAAHAAGADIVISNKVPLSREAIAALPELRLVAIAATGYNHVDL
DACRERGVAVCNIRHYGDHTVAEHAFMLMMALMKNLPAYQRDVAAGVWSQSRQFCHFGAPIREVRGATLAIVGSGGIGGR
LAQMARAFGMEVLFAERKGATRVREERVPFDEALARADVVSLHCPLTEETRGMIAQPELMAMKPGAILINTARGGLVDEA
DLVAALKYGQLGGAGFDVLSSEPPSPDNPLLKARLPNLIVTPHVGWASGEAMRRLAAQLVANIEAFVDGRKVNRLI

Sequences:

>Translated_316_residues
MTHNVVFLDRDSLPVPVPVFGFPVHYREYPATASEQLAAHAAGADIVISNKVPLSREAIAALPELRLVAIAATGYNHVDL
DACRERGVAVCNIRHYGDHTVAEHAFMLMMALMKNLPAYQRDVAAGVWSQSRQFCHFGAPIREVRGATLAIVGSGGIGGR
LAQMARAFGMEVLFAERKGATRVREERVPFDEALARADVVSLHCPLTEETRGMIAQPELMAMKPGAILINTARGGLVDEA
DLVAALKYGQLGGAGFDVLSSEPPSPDNPLLKARLPNLIVTPHVGWASGEAMRRLAAQLVANIEAFVDGRKVNRLI
>Mature_315_residues
THNVVFLDRDSLPVPVPVFGFPVHYREYPATASEQLAAHAAGADIVISNKVPLSREAIAALPELRLVAIAATGYNHVDLD
ACRERGVAVCNIRHYGDHTVAEHAFMLMMALMKNLPAYQRDVAAGVWSQSRQFCHFGAPIREVRGATLAIVGSGGIGGRL
AQMARAFGMEVLFAERKGATRVREERVPFDEALARADVVSLHCPLTEETRGMIAQPELMAMKPGAILINTARGGLVDEAD
LVAALKYGQLGGAGFDVLSSEPPSPDNPLLKARLPNLIVTPHVGWASGEAMRRLAAQLVANIEAFVDGRKVNRLI

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=283, Percent_Identity=34.2756183745583, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4557499, Length=283, Percent_Identity=34.2756183745583, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI61743967, Length=283, Percent_Identity=33.2155477031802, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4557497, Length=283, Percent_Identity=33.2155477031802, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI145580575, Length=283, Percent_Identity=34.2756183745583, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI23308577, Length=294, Percent_Identity=27.891156462585, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI6912396, Length=280, Percent_Identity=30.7142857142857, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI87082289, Length=281, Percent_Identity=32.3843416370107, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789279, Length=287, Percent_Identity=30.6620209059233, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=27.6073619631902, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87081824, Length=144, Percent_Identity=37.5, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788660, Length=276, Percent_Identity=26.8115942028986, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=273, Percent_Identity=31.1355311355311, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=279, Percent_Identity=32.258064516129, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=281, Percent_Identity=31.6725978647687, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322116, Length=281, Percent_Identity=30.9608540925267, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324055, Length=272, Percent_Identity=28.3088235294118, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=272, Percent_Identity=29.0441176470588, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324980, Length=183, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646446, Length=313, Percent_Identity=32.9073482428115, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646448, Length=313, Percent_Identity=32.9073482428115, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646452, Length=313, Percent_Identity=32.9073482428115, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646450, Length=313, Percent_Identity=32.9073482428115, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI62472511, Length=290, Percent_Identity=34.4827586206897, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI28571528, Length=258, Percent_Identity=34.1085271317829, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45551003, Length=243, Percent_Identity=31.2757201646091, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28574286, Length=235, Percent_Identity=31.063829787234, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585514, Length=243, Percent_Identity=31.2757201646091, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574282, Length=243, Percent_Identity=31.2757201646091, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574284, Length=243, Percent_Identity=31.2757201646091, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45552429, Length=243, Percent_Identity=31.2757201646091, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585516, Length=323, Percent_Identity=26.0061919504644, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921140, Length=263, Percent_Identity=28.5171102661597, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.29

Molecular weight: Translated: 34040; Mature: 33909

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHNVVFLDRDSLPVPVPVFGFPVHYREYPATASEQLAAHAAGADIVISNKVPLSREAIA
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHH
ALPELRLVAIAATGYNHVDLDACRERGVAVCNIRHYGDHTVAEHAFMLMMALMKNLPAYQ
HCCCEEEEEEEECCCCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHH
RDVAAGVWSQSRQFCHFGAPIREVRGATLAIVGSGGIGGRLAQMARAFGMEVLFAERKGA
HHHHHHHHHHCCCCEECCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCC
TRVREERVPFDEALARADVVSLHCPLTEETRGMIAQPELMAMKPGAILINTARGGLVDEA
HHHHHHCCCHHHHHHHHCEEEEECCCCHHHCCCCCCCHHEEECCCEEEEECCCCCCCCHH
DLVAALKYGQLGGAGFDVLSSEPPSPDNPLLKARLPNLIVTPHVGWASGEAMRRLAAQLV
HHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
ANIEAFVDGRKVNRLI
HHHHHHHCCHHHHCCC
>Mature Secondary Structure 
THNVVFLDRDSLPVPVPVFGFPVHYREYPATASEQLAAHAAGADIVISNKVPLSREAIA
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHH
ALPELRLVAIAATGYNHVDLDACRERGVAVCNIRHYGDHTVAEHAFMLMMALMKNLPAYQ
HCCCEEEEEEEECCCCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHH
RDVAAGVWSQSRQFCHFGAPIREVRGATLAIVGSGGIGGRLAQMARAFGMEVLFAERKGA
HHHHHHHHHHCCCCEECCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCC
TRVREERVPFDEALARADVVSLHCPLTEETRGMIAQPELMAMKPGAILINTARGGLVDEA
HHHHHHCCCHHHHHHHHCEEEEECCCCHHHCCCCCCCHHEEECCCEEEEECCCCCCCCHH
DLVAALKYGQLGGAGFDVLSSEPPSPDNPLLKARLPNLIVTPHVGWASGEAMRRLAAQLV
HHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHCCCCCEEEECCCCCCCHHHHHHHHHHHH
ANIEAFVDGRKVNRLI
HHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-glycerate; NAD+

Specific reaction: (R)-glycerate + NAD+ = hydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]