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The map label for this gene is thiG

Identifier: 34499221

GI number: 34499221

Start: 4064760

End: 4065554

Strand: Reverse

Name: thiG

Synonym: CV_3766

Alternate gene names: 34499221

Gene position: 4065554-4064760 (Counterclockwise)

Preceding gene: 34499222

Following gene: 34499220

Centisome position: 85.57

GC content: 62.26

Gene sequence:

>795_bases
GTGGACGACAAACTGGTGATAGCCGGCAGGGAGTACGGCTCTCGCTTGCTGGTGGGGACCGGCAAATACAAGGATTTCGA
GCAGACCGCGGCGGCGCTGGACGTGTCCGGCACCGAAATCGTCACCGCCGCGATAAGGCGGGTGAATCTGGGGCAGAACG
CCAACGAGCCGAATTTGCTGGACTTTCTGCCGAAAAACCGGTATAACCTGCTGCCCAACACGGCGGGGTGCTATTCCGCC
GAGGATGCGATCCGCACCCTGCGCCTGGCGCGCGAGCTTCTGGACGACCATCGTTTCGTGAAGCTGGAGGTGCTGGGCGA
TCCCAACAACCTGTATCCCAACGTCAGGGAAACGCTGAAAGCCGCGGAAGTGCTAGTGGCCGAAGGCTTCGACGTGTTGG
TGTACACCTCGGACGATCCCATCGTCGCGCGCGAGCTTGAGCAGATTGGCTGCTGCGCGATCATGCCGCTGGCCAGCCTG
ATCGGCTCCGGCATGGGCATCCTGAATCCGTGGAATCTGCAATTGATTATCGAGCAGTCCAAGGTCCCCGTGATCGTGGA
CGCCGGCGTCGGCACCGCGTCCGACGCGGCGATCGCGATGGAGCTGGGCTGCGATGGCGTGCTGATGAATACCGCGATTG
CCGCTGCGCGCGATCCGGTCCGAATGGCGCACGCGATGAAATTGGCCGTGGAGGCAGGCCGCGCCGCGTATCTCGCGGGC
CGGATGCCGAAGCGTTTTTACAGTGCAGTGCCAAGTTCTCCTAGTGAGGGGGTGATTTCTTCCGTCAAGTCGTGA

Upstream 100 bases:

>100_bases
GCTGGCGGACGGCGACGTGCTGGAAATCGTGGTGGCGGTCGGCGGCGGCTGAAACCGTCTGTCCGCCATTCTAAGGCCAA
TGGAAGAAAAGGGAATGCAG

Downstream 100 bases:

>100_bases
TGTTCATGGCCCAGGCTGGCCGCGCCAAACCGCGCAAAACACCACAAAAGACCTGACGTTGCCGTTTCAGACTTTTACAA
ATGCAGTAACATTGCTAAAA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDDKLVIAGREYGSRLLVGTGKYKDFEQTAAALDVSGTEIVTAAIRRVNLGQNANEPNLLDFLPKNRYNLLPNTAGCYSA
EDAIRTLRLARELLDDHRFVKLEVLGDPNNLYPNVRETLKAAEVLVAEGFDVLVYTSDDPIVARELEQIGCCAIMPLASL
IGSGMGILNPWNLQLIIEQSKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAARDPVRMAHAMKLAVEAGRAAYLAG
RMPKRFYSAVPSSPSEGVISSVKS

Sequences:

>Translated_264_residues
MDDKLVIAGREYGSRLLVGTGKYKDFEQTAAALDVSGTEIVTAAIRRVNLGQNANEPNLLDFLPKNRYNLLPNTAGCYSA
EDAIRTLRLARELLDDHRFVKLEVLGDPNNLYPNVRETLKAAEVLVAEGFDVLVYTSDDPIVARELEQIGCCAIMPLASL
IGSGMGILNPWNLQLIIEQSKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAARDPVRMAHAMKLAVEAGRAAYLAG
RMPKRFYSAVPSSPSEGVISSVKS
>Mature_264_residues
MDDKLVIAGREYGSRLLVGTGKYKDFEQTAAALDVSGTEIVTAAIRRVNLGQNANEPNLLDFLPKNRYNLLPNTAGCYSA
EDAIRTLRLARELLDDHRFVKLEVLGDPNNLYPNVRETLKAAEVLVAEGFDVLVYTSDDPIVARELEQIGCCAIMPLASL
IGSGMGILNPWNLQLIIEQSKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAARDPVRMAHAMKLAVEAGRAAYLAG
RMPKRFYSAVPSSPSEGVISSVKS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=257, Percent_Identity=45.5252918287938, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_CHRVO (Q7NRL4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903436.1
- ProteinModelPortal:   Q7NRL4
- SMR:   Q7NRL4
- GeneID:   2548649
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3766
- NMPDR:   fig|243365.1.peg.3766
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q7NRL4
- ProtClustDB:   PRK00208
- BioCyc:   CVIO243365:CV_3766-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28304; Mature: 28304

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: ACT_SITE 101-101 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDKLVIAGREYGSRLLVGTGKYKDFEQTAAALDVSGTEIVTAAIRRVNLGQNANEPNLL
CCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHH
DFLPKNRYNLLPNTAGCYSAEDAIRTLRLARELLDDHRFVKLEVLGDPNNLYPNVRETLK
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHH
AAEVLVAEGFDVLVYTSDDPIVARELEQIGCCAIMPLASLIGSGMGILNPWNLQLIIEQS
HHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEECC
KVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAARDPVRMAHAMKLAVEAGRAAYLAG
CCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHC
RMPKRFYSAVPSSPSEGVISSVKS
CCHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MDDKLVIAGREYGSRLLVGTGKYKDFEQTAAALDVSGTEIVTAAIRRVNLGQNANEPNLL
CCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHH
DFLPKNRYNLLPNTAGCYSAEDAIRTLRLARELLDDHRFVKLEVLGDPNNLYPNVRETLK
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHH
AAEVLVAEGFDVLVYTSDDPIVARELEQIGCCAIMPLASLIGSGMGILNPWNLQLIIEQS
HHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEECC
KVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAARDPVRMAHAMKLAVEAGRAAYLAG
CCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHC
RMPKRFYSAVPSSPSEGVISSVKS
CCHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782