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The map label for this gene is fabI [H]

Identifier: 34499198

GI number: 34499198

Start: 4041755

End: 4042531

Strand: Direct

Name: fabI [H]

Synonym: CV_3743

Alternate gene names: 34499198

Gene position: 4041755-4042531 (Clockwise)

Preceding gene: 34499191

Following gene: 34499199

Centisome position: 85.07

GC content: 64.86

Gene sequence:

>777_bases
ATGGAAAGCATACGCAACATTCTCGCCGGCAAGAGGGGGCTCATCGTCGGCATCGCCAACGAGAACAGCATCGCCTACGG
CTGCGCCAGCGTGCTGCGCCAGCTGGGGGCCGAATGCGCGGTCACCTACCTCAACGCCAAGGCCGAGAAATACGTGCGGC
CGCTGGCCGAGCAACTGCAGGCCAAGCTGGTGCTGCCGCTGGACGTGGAGCAGCCGGGCCAGTTGGAAAGCGTATTCGCC
GAGATAGAAAAGCGCTGGGGCGAGCTGGATTTCATCGTCCACTCCATCGCCTACTGCCCGATGGAGGACTTGCACGGCCG
CGTCACCGACTGTTCGCTGGCCGGCTTCCAGCAGGCGATGCGGGTGTCCTGCTACTCCTTCATCGAGATGGCGCGCCTGG
CCGAGCCGCTGATGAAGCGCGGCGGCAGCCTGATCACCATGAGCTACTACGGTGCGGACAAGGTGGTGGAGCACTACAAC
ATCATGGGCCCGGTCAAGGCCGCGCTGGAAAGCGTGTCCCGCTACCTGGCCAACGAGCTCGGCCCCAAGCGCATCCGCGT
GCATGCGGTGTCGCCCGGCCCGCTGAAGACGCGCGCCGCCTCCGGCATCGCGCATTTCGACCAGTTGATCGAGGACGCTA
TCGCCCACGCGCCGCAGAATCGCCTGGTGGAGATAGAGGAAGTGGGCATGACCTGCGCCTTCCTGATTTCGGACGCCGCC
AGCGGCCTCACCGGCCAGACCATCTACGTAGACGGCGGACACCACATCAAGGCTTGA

Upstream 100 bases:

>100_bases
CGACTGAAACGACAATACAAAAACCACTTTCAATCATTCTGCTGCAACACCGCCCTGCAACGCTATCCCGGAGGTCCAAA
TGGTTTACCAAGAAGGTCAG

Downstream 100 bases:

>100_bases
GGAGAGCGGCATGATTCACGATACCCACGCATTCGACGACATCCTGCAGCAGGCGCGGCAACTGGGCGCGCTGCCGATGG
CGGTGGCCCACCCCTGCAGC

Product: enoyl-ACP reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MESIRNILAGKRGLIVGIANENSIAYGCASVLRQLGAECAVTYLNAKAEKYVRPLAEQLQAKLVLPLDVEQPGQLESVFA
EIEKRWGELDFIVHSIAYCPMEDLHGRVTDCSLAGFQQAMRVSCYSFIEMARLAEPLMKRGGSLITMSYYGADKVVEHYN
IMGPVKAALESVSRYLANELGPKRIRVHAVSPGPLKTRAASGIAHFDQLIEDAIAHAPQNRLVEIEEVGMTCAFLISDAA
SGLTGQTIYVDGGHHIKA

Sequences:

>Translated_258_residues
MESIRNILAGKRGLIVGIANENSIAYGCASVLRQLGAECAVTYLNAKAEKYVRPLAEQLQAKLVLPLDVEQPGQLESVFA
EIEKRWGELDFIVHSIAYCPMEDLHGRVTDCSLAGFQQAMRVSCYSFIEMARLAEPLMKRGGSLITMSYYGADKVVEHYN
IMGPVKAALESVSRYLANELGPKRIRVHAVSPGPLKTRAASGIAHFDQLIEDAIAHAPQNRLVEIEEVGMTCAFLISDAA
SGLTGQTIYVDGGHHIKA
>Mature_258_residues
MESIRNILAGKRGLIVGIANENSIAYGCASVLRQLGAECAVTYLNAKAEKYVRPLAEQLQAKLVLPLDVEQPGQLESVFA
EIEKRWGELDFIVHSIAYCPMEDLHGRVTDCSLAGFQQAMRVSCYSFIEMARLAEPLMKRGGSLITMSYYGADKVVEHYN
IMGPVKAALESVSRYLANELGPKRIRVHAVSPGPLKTRAASGIAHFDQLIEDAIAHAPQNRLVEIEEVGMTCAFLISDAA
SGLTGQTIYVDGGHHIKA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=241, Percent_Identity=25.3112033195021, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI7705925, Length=261, Percent_Identity=24.904214559387, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=27.34375, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI59889578, Length=219, Percent_Identity=25.5707762557078, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=42.2924901185771, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=28.740157480315, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=25.390625, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=26.171875, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=112, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=180, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=271, Percent_Identity=26.9372693726937, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=27.9245283018868, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=25.8687258687259, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=26.7441860465116, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21355319, Length=251, Percent_Identity=26.6932270916335, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI23397609, Length=237, Percent_Identity=24.8945147679325, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=146, Percent_Identity=28.0821917808219, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571526, Length=202, Percent_Identity=29.2079207920792, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=195, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28116; Mature: 28116

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIRNILAGKRGLIVGIANENSIAYGCASVLRQLGAECAVTYLNAKAEKYVRPLAEQLQ
CHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AKLVLPLDVEQPGQLESVFAEIEKRWGELDFIVHSIAYCPMEDLHGRVTDCSLAGFQQAM
HEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHH
RVSCYSFIEMARLAEPLMKRGGSLITMSYYGADKVVEHYNIMGPVKAALESVSRYLANEL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GPKRIRVHAVSPGPLKTRAASGIAHFDQLIEDAIAHAPQNRLVEIEEVGMTCAFLISDAA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCHHHHHHHHHHC
SGLTGQTIYVDGGHHIKA
CCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MESIRNILAGKRGLIVGIANENSIAYGCASVLRQLGAECAVTYLNAKAEKYVRPLAEQLQ
CHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AKLVLPLDVEQPGQLESVFAEIEKRWGELDFIVHSIAYCPMEDLHGRVTDCSLAGFQQAM
HEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHH
RVSCYSFIEMARLAEPLMKRGGSLITMSYYGADKVVEHYNIMGPVKAALESVSRYLANEL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GPKRIRVHAVSPGPLKTRAASGIAHFDQLIEDAIAHAPQNRLVEIEEVGMTCAFLISDAA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCHHHHHHHHHHC
SGLTGQTIYVDGGHHIKA
CCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA