Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 34499193

GI number: 34499193

Start: 4036470

End: 4037651

Strand: Reverse

Name: pleD [H]

Synonym: CV_3738

Alternate gene names: 34499193

Gene position: 4037651-4036470 (Counterclockwise)

Preceding gene: 34499194

Following gene: 34499192

Centisome position: 84.98

GC content: 64.81

Gene sequence:

>1182_bases
ATGGTGGAGCGGGAATCCAGGCTGCAGGTGATGCGCGCCTCCGGCGACTGCCTGCCCTATGCCTTTCCCGGCGTGCTGGT
GCTGCAGGCGCTGCAATGGGGCCATGCGCCCACCATTGGCTTGCTGCGCTGGGGCATGCTCATCTATCCGCTGCTGGCGC
TGGAGTACCTGATCACCATGCTGGCCAGGCGTTGCCGCGACGACGACGACGACCGCGTCGCCGTCCTGTATCGCAGCTTT
GTCGCCTGCCTGCTGGCGAGCAGCCTGGGATGGGGCAGCTCGGCCTGGCTGATCATGCGCGACGACGACATCACCTACGC
CTTGGTGGTATTGCTGTGGATGCTGGCGATCAGCGCGGTGCAGGGCGCGTTGCTGATCGGCAAGCGCAGGCTGTTCTACC
TGTTCGAAGGAATCTTGGGGGCGCTGATGCTGACGCGTCTGTTCGACTCCCATGACCAGCTGCTGCACCTGGTCGGGGTG
TCTGTGCTGGTCTTCAGCGGCGTGCTGATCCAGTTCACCGCCCATCTGCATCGTTTCCTGGTGGAATCGATCAAGAATCG
GGTCGATAACCAGCTGTTGTCCTGGCGGCTGGGAGAGGAACGCGCGCAGCTGGACGACTGCAATGCGCAGCTGGAGCGGC
AGAACGACGAGCTCGACGCCACCTTGCTGCGGATACGCGAGCTGACGGTTCGCGATCCGTTGACCGGTGTCTTCAACATG
CGGGCGTTGACGCCCGAGCTGGAGCGCCTGCTGCAGCGTTCCGAGGAGCAGGGCGCGCCGTTCGCGCTGTCCATCATCGA
CCTGGATTACTTCAAACGCATCAACGACGAATGCGGCCACCCGACCGGCGACGAGGTGCTGCAGCAGCTGTGCCGGATCG
TCAGCGCCACGCTGAAGCCAGGCGAAGTGTTCGCCCGCTACGGCGGCGAGGAATTCCTGCTCGTGACGCCGGATTGCAAC
GGCGGCGAGCATTTCCGCCGCATGGACGCCTTGCGCTCGGCCATCGCCGATTACGACTGGACGCCATTGACCGGCATCCT
GCCGGTGACCATCTCCTGCGGGGTGGCCAGCTGGTATCCGGGCACCGATCTGGGCGCGATGCTGCAGCGGGCGGACAGCG
CCTTGTACATGGCCAAGGCGCAGGGGCGCAATCGGGTCTGCTCCGAGAAGGACGCGATATGA

Upstream 100 bases:

>100_bases
GCGCAAGCCGGCGGGGTGTTGTTTTATCGACCGCCGCCGTCCCCTATAACAATAGTATTCATAGGGTTAGGGGGATGGAG
TGCAAGCGACCAGGCGGCGA

Downstream 100 bases:

>100_bases
CGCGGATCGATCGCGAGCCTATCGTCTTCGGCCGGCAGGAGGCGGAGCTGGCGCGGGAGATGGTGGGCATGCTCAGGCTG
TCGATTTGGCAATCCTTCCT

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVERESRLQVMRASGDCLPYAFPGVLVLQALQWGHAPTIGLLRWGMLIYPLLALEYLITMLARRCRDDDDDRVAVLYRSF
VACLLASSLGWGSSAWLIMRDDDITYALVVLLWMLAISAVQGALLIGKRRLFYLFEGILGALMLTRLFDSHDQLLHLVGV
SVLVFSGVLIQFTAHLHRFLVESIKNRVDNQLLSWRLGEERAQLDDCNAQLERQNDELDATLLRIRELTVRDPLTGVFNM
RALTPELERLLQRSEEQGAPFALSIIDLDYFKRINDECGHPTGDEVLQQLCRIVSATLKPGEVFARYGGEEFLLVTPDCN
GGEHFRRMDALRSAIADYDWTPLTGILPVTISCGVASWYPGTDLGAMLQRADSALYMAKAQGRNRVCSEKDAI

Sequences:

>Translated_393_residues
MVERESRLQVMRASGDCLPYAFPGVLVLQALQWGHAPTIGLLRWGMLIYPLLALEYLITMLARRCRDDDDDRVAVLYRSF
VACLLASSLGWGSSAWLIMRDDDITYALVVLLWMLAISAVQGALLIGKRRLFYLFEGILGALMLTRLFDSHDQLLHLVGV
SVLVFSGVLIQFTAHLHRFLVESIKNRVDNQLLSWRLGEERAQLDDCNAQLERQNDELDATLLRIRELTVRDPLTGVFNM
RALTPELERLLQRSEEQGAPFALSIIDLDYFKRINDECGHPTGDEVLQQLCRIVSATLKPGEVFARYGGEEFLLVTPDCN
GGEHFRRMDALRSAIADYDWTPLTGILPVTISCGVASWYPGTDLGAMLQRADSALYMAKAQGRNRVCSEKDAI
>Mature_393_residues
MVERESRLQVMRASGDCLPYAFPGVLVLQALQWGHAPTIGLLRWGMLIYPLLALEYLITMLARRCRDDDDDRVAVLYRSF
VACLLASSLGWGSSAWLIMRDDDITYALVVLLWMLAISAVQGALLIGKRRLFYLFEGILGALMLTRLFDSHDQLLHLVGV
SVLVFSGVLIQFTAHLHRFLVESIKNRVDNQLLSWRLGEERAQLDDCNAQLERQNDELDATLLRIRELTVRDPLTGVFNM
RALTPELERLLQRSEEQGAPFALSIIDLDYFKRINDECGHPTGDEVLQQLCRIVSATLKPGEVFARYGGEEFLLVTPDCN
GGEHFRRMDALRSAIADYDWTPLTGILPVTISCGVASWYPGTDLGAMLQRADSALYMAKAQGRNRVCSEKDAI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=35.5029585798817, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87081881, Length=158, Percent_Identity=33.5443037974684, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=32.7380952380952, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=33.5365853658537, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=31.7073170731707, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=34.1772151898734, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788956, Length=236, Percent_Identity=29.6610169491525, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=205, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=30.3571428571429, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=31.7647058823529, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=26.25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=31.6455696202532, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44208; Mature: 44208

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERESRLQVMRASGDCLPYAFPGVLVLQALQWGHAPTIGLLRWGMLIYPLLALEYLITM
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LARRCRDDDDDRVAVLYRSFVACLLASSLGWGSSAWLIMRDDDITYALVVLLWMLAISAV
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
QGALLIGKRRLFYLFEGILGALMLTRLFDSHDQLLHLVGVSVLVFSGVLIQFTAHLHRFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESIKNRVDNQLLSWRLGEERAQLDDCNAQLERQNDELDATLLRIRELTVRDPLTGVFNM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
RALTPELERLLQRSEEQGAPFALSIIDLDYFKRINDECGHPTGDEVLQQLCRIVSATLKP
HHCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
GEVFARYGGEEFLLVTPDCNGGEHFRRMDALRSAIADYDWTPLTGILPVTISCGVASWYP
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCC
GTDLGAMLQRADSALYMAKAQGRNRVCSEKDAI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVERESRLQVMRASGDCLPYAFPGVLVLQALQWGHAPTIGLLRWGMLIYPLLALEYLITM
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LARRCRDDDDDRVAVLYRSFVACLLASSLGWGSSAWLIMRDDDITYALVVLLWMLAISAV
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
QGALLIGKRRLFYLFEGILGALMLTRLFDSHDQLLHLVGVSVLVFSGVLIQFTAHLHRFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESIKNRVDNQLLSWRLGEERAQLDDCNAQLERQNDELDATLLRIRELTVRDPLTGVFNM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
RALTPELERLLQRSEEQGAPFALSIIDLDYFKRINDECGHPTGDEVLQQLCRIVSATLKP
HHCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
GEVFARYGGEEFLLVTPDCNGGEHFRRMDALRSAIADYDWTPLTGILPVTISCGVASWYP
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCC
GTDLGAMLQRADSALYMAKAQGRNRVCSEKDAI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA