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The map label for this gene is holB [H]

Identifier: 34499177

GI number: 34499177

Start: 4020502

End: 4021506

Strand: Reverse

Name: holB [H]

Synonym: CV_3722

Alternate gene names: 34499177

Gene position: 4021506-4020502 (Counterclockwise)

Preceding gene: 34499178

Following gene: 34499176

Centisome position: 84.64

GC content: 70.05

Gene sequence:

>1005_bases
ATGCTGTACCCGTGGCAGAGCGCGGATTGGACGCGCCTTTCCGCCGAGCGCGAGCGCATGCCCGGCGCCTGGCTCTTCAC
CGGCGCCGCCGGCATCGGCAAGCTGGCTTTCGCCGAGCACTTGGCGCAATCGCTGCTGTGCGAGAGCCCGGCGGCCGGCC
ACCAGCCGTGCGGCCAATGCCAGGGCTGCCGCTGGTTCCATAGCGGCACCCACCCCGATTTCCGCCGCCTGTCGCCGTTC
GCCGACGAGGAGGAGGAGGGCAAGGAGGCCAAGACCACGCGCAAGCTGCCGCAGATCAAGATCGAGGCGGTGCGCGAGGT
GATCGATTTCGCCCACCTGACCGCCCATCGCGCCGGCCGCCGCGTGATCCTGGTGGAGCCGGCCGAGAGCCTGAACCTGG
CCGCAGCCAACGCGCTGCTGAAGATACTGGAAGAGCCGCCGGAGGCGGTGCTGTTCCTGCTGGTGTCGCACGCGCCGCAG
CGCTTGCTGCCCACCATCCGCAGCCGCTGCCGCCAGTTCGCGCTGACCCGGCCGGCCGAGAGCGCCGCGCTGGCGTGGCT
GAAGGAGCAGGGCGTCGCCAACGCCGAGGCGGAACTGGCGCACAATGGCGGCGCGCCGCTGTTCGACCACGATCCCGAGC
TGGCCGCGCTGCGCGGCCAGTTCGTCCGAGGCCTGGGCCAGCCCAGTTTCGCCGGCATGCTGCAATTGGCCGAGCTGATC
GACAAGAACAAGCAGCTGTCGCTGCTGCAGCCCTTGTCCTGGCTGCAGAAGTGGCTGCACGACCTGGCGGCGCTGTGCCT
GGCCGGCCGCGTCCGCTATTATCCAGATCATGAGCGCGCGCTCGCCGCGCTGGCGCCGCGCTGCGACGCCGTCCAGCTGA
TGGCCTGCCAGCAGGCGCTGACGGCGCTGGCGCCGTTTTCCCAGCATACGCTGAATGCCCGCCTGCAACTGGAGGCGGTG
TTGATGGATTACCTGAAGATCTTCGCCGGCGGCAAGGCCGGCTGA

Upstream 100 bases:

>100_bases
AGGCGCCGCAGCGCATCGCCGTGCTGGACGCCAGCCGCGGCATCGCCGACATCCAGGCTGACATCGTGCTCCACCTGGAA
CGCCTGCTGGAGCGCGGCTG

Downstream 100 bases:

>100_bases
GCAAGAAGGGGCCCGCATGGATGCCGCCAACCACCGGCCGGATCTGAACAATCCCAATCGCCCCGGCGTGCTGTCGCTGC
ATATCAAGGAGCGCAGCGCG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MLYPWQSADWTRLSAERERMPGAWLFTGAAGIGKLAFAEHLAQSLLCESPAAGHQPCGQCQGCRWFHSGTHPDFRRLSPF
ADEEEEGKEAKTTRKLPQIKIEAVREVIDFAHLTAHRAGRRVILVEPAESLNLAAANALLKILEEPPEAVLFLLVSHAPQ
RLLPTIRSRCRQFALTRPAESAALAWLKEQGVANAEAELAHNGGAPLFDHDPELAALRGQFVRGLGQPSFAGMLQLAELI
DKNKQLSLLQPLSWLQKWLHDLAALCLAGRVRYYPDHERALAALAPRCDAVQLMACQQALTALAPFSQHTLNARLQLEAV
LMDYLKIFAGGKAG

Sequences:

>Translated_334_residues
MLYPWQSADWTRLSAERERMPGAWLFTGAAGIGKLAFAEHLAQSLLCESPAAGHQPCGQCQGCRWFHSGTHPDFRRLSPF
ADEEEEGKEAKTTRKLPQIKIEAVREVIDFAHLTAHRAGRRVILVEPAESLNLAAANALLKILEEPPEAVLFLLVSHAPQ
RLLPTIRSRCRQFALTRPAESAALAWLKEQGVANAEAELAHNGGAPLFDHDPELAALRGQFVRGLGQPSFAGMLQLAELI
DKNKQLSLLQPLSWLQKWLHDLAALCLAGRVRYYPDHERALAALAPRCDAVQLMACQQALTALAPFSQHTLNARLQLEAV
LMDYLKIFAGGKAG
>Mature_334_residues
MLYPWQSADWTRLSAERERMPGAWLFTGAAGIGKLAFAEHLAQSLLCESPAAGHQPCGQCQGCRWFHSGTHPDFRRLSPF
ADEEEEGKEAKTTRKLPQIKIEAVREVIDFAHLTAHRAGRRVILVEPAESLNLAAANALLKILEEPPEAVLFLLVSHAPQ
RLLPTIRSRCRQFALTRPAESAALAWLKEQGVANAEAELAHNGGAPLFDHDPELAALRGQFVRGLGQPSFAGMLQLAELI
DKNKQLSLLQPLSWLQKWLHDLAALCLAGRVRYYPDHERALAALAPRCDAVQLMACQQALTALAPFSQHTLNARLQLEAV
LMDYLKIFAGGKAG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=190, Percent_Identity=40, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786676, Length=157, Percent_Identity=37.5796178343949, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36775; Mature: 36775

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPWQSADWTRLSAERERMPGAWLFTGAAGIGKLAFAEHLAQSLLCESPAAGHQPCGQC
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QGCRWFHSGTHPDFRRLSPFADEEEEGKEAKTTRKLPQIKIEAVREVIDFAHLTAHRAGR
CCCEECCCCCCCCHHHCCCCCCCHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
RVILVEPAESLNLAAANALLKILEEPPEAVLFLLVSHAPQRLLPTIRSRCRQFALTRPAE
EEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCH
SAALAWLKEQGVANAEAELAHNGGAPLFDHDPELAALRGQFVRGLGQPSFAGMLQLAELI
HHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DKNKQLSLLQPLSWLQKWLHDLAALCLAGRVRYYPDHERALAALAPRCDAVQLMACQQAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
TALAPFSQHTLNARLQLEAVLMDYLKIFAGGKAG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLYPWQSADWTRLSAERERMPGAWLFTGAAGIGKLAFAEHLAQSLLCESPAAGHQPCGQC
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QGCRWFHSGTHPDFRRLSPFADEEEEGKEAKTTRKLPQIKIEAVREVIDFAHLTAHRAGR
CCCEECCCCCCCCHHHCCCCCCCHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
RVILVEPAESLNLAAANALLKILEEPPEAVLFLLVSHAPQRLLPTIRSRCRQFALTRPAE
EEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCH
SAALAWLKEQGVANAEAELAHNGGAPLFDHDPELAALRGQFVRGLGQPSFAGMLQLAELI
HHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DKNKQLSLLQPLSWLQKWLHDLAALCLAGRVRYYPDHERALAALAPRCDAVQLMACQQAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
TALAPFSQHTLNARLQLEAVLMDYLKIFAGGKAG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]