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The map label for this gene is lipB [H]

Identifier: 34499173

GI number: 34499173

Start: 4018086

End: 4018838

Strand: Reverse

Name: lipB [H]

Synonym: CV_3718

Alternate gene names: 34499173

Gene position: 4018838-4018086 (Counterclockwise)

Preceding gene: 34499174

Following gene: 34499168

Centisome position: 84.59

GC content: 66.93

Gene sequence:

>753_bases
CTGGGCTGCGGCTCCAGTTCGGGCACCCCGGCGATAGGCTGCGGCTGCCCCACCTGCCAGTCGGACGATGTCCGCAACAA
GCGCACCCGCGCCAGCGCCTATGTGCGCGTCGGGGAGCTGGGTTTCCTGATCGACACCGGGCCGGATCTGCGCCAGCAGG
CTCTGCGCGAAGGCGTGAGCCGGCTGGACGCGGTGCTCTATACCCATCCGCACGCCGACCACCTGAACGGCATCGACGAT
CTGCGCGCCTTCTGTTATCTGAAGCAGGGGCCGATCGCGCTGTACGGCAACGACTTCACCATCGCCAACATCCGCGAGCG
CTTCGGCTACGCGCTGCAGCCGCCGTCCAGGATGTGGGACAAGCCGGTGTTGCTGCCGGAGGTGGTCAACGGGCCGTTCC
AGCACCAGGGCGTGACGCTGACCCCCATTCCCCTGCTGCACGGCAGCTGGCCCTGCCTGGGATGGCGCATCGGCGACGTG
GCCTGGCTGACCGATCTGTCGCGGATTCCCGACAGCAGCCTGCCTTTGCTGGAAGGCTTGGAGCTGCTGTTCCTCGATTG
CCTGAACGAAGATCCCTATCCGTCCCACCTGTCGGTGGGAGAGGCCTTCGAGTGGGCCGCTCGCATCGCCGCGCGCCGCA
CGGTGCTGATCCACATGACGCACAAGCTGGAATACCTCGATCTCAGCCAGCGCTGTCCGCCCGGCGTGGAGGTCGGTTTC
GACGGCTGGCGCGCGGAAGTGGCTATGGAATGA

Upstream 100 bases:

>100_bases
CCCTTATGCGACGGCGGTTCGACGGCGGCGCCGGCGGCGCGCGCGCAGCCGTGCCAGTGATCAAGCAAGGAGAGTCGTGA
ACACGCTAGAAGTCACCATA

Downstream 100 bases:

>100_bases
TGAATATCATGTAAAAAAACATGATCTTTATCAATGGTTTGTGAGGTTCACTTCTTGAGCGGTATCTTCTGGTCCAGCAC
CGCCACCGCGGTGATCGCGT

Product: hydrolase protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MGCGSSSGTPAIGCGCPTCQSDDVRNKRTRASAYVRVGELGFLIDTGPDLRQQALREGVSRLDAVLYTHPHADHLNGIDD
LRAFCYLKQGPIALYGNDFTIANIRERFGYALQPPSRMWDKPVLLPEVVNGPFQHQGVTLTPIPLLHGSWPCLGWRIGDV
AWLTDLSRIPDSSLPLLEGLELLFLDCLNEDPYPSHLSVGEAFEWAARIAARRTVLIHMTHKLEYLDLSQRCPPGVEVGF
DGWRAEVAME

Sequences:

>Translated_250_residues
MGCGSSSGTPAIGCGCPTCQSDDVRNKRTRASAYVRVGELGFLIDTGPDLRQQALREGVSRLDAVLYTHPHADHLNGIDD
LRAFCYLKQGPIALYGNDFTIANIRERFGYALQPPSRMWDKPVLLPEVVNGPFQHQGVTLTPIPLLHGSWPCLGWRIGDV
AWLTDLSRIPDSSLPLLEGLELLFLDCLNEDPYPSHLSVGEAFEWAARIAARRTVLIHMTHKLEYLDLSQRCPPGVEVGF
DGWRAEVAME
>Mature_249_residues
GCGSSSGTPAIGCGCPTCQSDDVRNKRTRASAYVRVGELGFLIDTGPDLRQQALREGVSRLDAVLYTHPHADHLNGIDDL
RAFCYLKQGPIALYGNDFTIANIRERFGYALQPPSRMWDKPVLLPEVVNGPFQHQGVTLTPIPLLHGSWPCLGWRIGDVA
WLTDLSRIPDSSLPLLEGLELLFLDCLNEDPYPSHLSVGEAFEWAARIAARRTVLIHMTHKLEYLDLSQRCPPGVEVGFD
GWRAEVAME

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=254, Percent_Identity=27.5590551181102, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 27692; Mature: 27561

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCGSSSGTPAIGCGCPTCQSDDVRNKRTRASAYVRVGELGFLIDTGPDLRQQALREGVS
CCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHEEEECCEEEEEECCCHHHHHHHHHHHH
RLDAVLYTHPHADHLNGIDDLRAFCYLKQGPIALYGNDFTIANIRERFGYALQPPSRMWD
HHHHHHHCCCCHHHCCCHHHHHHHHEEECCCEEEECCCEEEEHHHHHCCCCCCCHHHHCC
KPVLLPEVVNGPFQHQGVTLTPIPLLHGSWPCLGWRIGDVAWLTDLSRIPDSSLPLLEGL
CCCCCHHHHCCCCCCCCEEEECCHHHCCCCCCCCEEECCHHHHHHHHHCCCCCCCHHHHH
ELLFLDCLNEDPYPSHLSVGEAFEWAARIAARRTVLIHMTHKLEYLDLSQRCPPGVEVGF
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCEEECHHHCCCCCCEECC
DGWRAEVAME
CCCEEEEECC
>Mature Secondary Structure 
GCGSSSGTPAIGCGCPTCQSDDVRNKRTRASAYVRVGELGFLIDTGPDLRQQALREGVS
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHEEEECCEEEEEECCCHHHHHHHHHHHH
RLDAVLYTHPHADHLNGIDDLRAFCYLKQGPIALYGNDFTIANIRERFGYALQPPSRMWD
HHHHHHHCCCCHHHCCCHHHHHHHHEEECCCEEEECCCEEEEHHHHHCCCCCCCHHHHCC
KPVLLPEVVNGPFQHQGVTLTPIPLLHGSWPCLGWRIGDVAWLTDLSRIPDSSLPLLEGL
CCCCCHHHHCCCCCCCCEEEECCHHHCCCCCCCCEEECCHHHHHHHHHCCCCCCCHHHHH
ELLFLDCLNEDPYPSHLSVGEAFEWAARIAARRTVLIHMTHKLEYLDLSQRCPPGVEVGF
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCEEECHHHCCCCCCEECC
DGWRAEVAME
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]