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The map label for this gene is lepB [H]

Identifier: 34499142

GI number: 34499142

Start: 3988898

End: 3989566

Strand: Direct

Name: lepB [H]

Synonym: CV_3687

Alternate gene names: 34499142

Gene position: 3988898-3989566 (Clockwise)

Preceding gene: 34499141

Following gene: 34499144

Centisome position: 83.96

GC content: 66.52

Gene sequence:

>669_bases
ATGAAAAACTGGCTGCAAAACAACCGCGGCTTTCTCGTCTTTTTGCTGCTGTTCGGCTTGTTCCGCACCGCGGTGGCGGA
CTGGAACCCGATCCCCTCCGGTTCGATGCGCCCGACGCTGCTGGAGGGCGACGTGGTGCTGGTCAACCGGCTGGCCTACG
ATCTGAAGCTGCCGCTGACCAACGTCGTCCTGCAGCAAACCGGCGAGCCGCAACGCGGCGACATCGTCACCTTCTACTCG
CCCAAGGACGGCAAGCATCTGATCAAGCGGCTGGTGGCGGTGCCGGGCGACACGGTGGAAATGCGCAGGGAGAGGCTGAT
CATCAATGGCCGCGCCGCCGATTACCAAGCTTTGCAGCAGGTGACGGAAACCGTGTCCGACCATGTCGCGCTGCCGGCGC
TGCGCCTGCGGGAAAGCGGCGCGCTGCCGGCCCACCGGGTGCAATGGCTGGCCGGCGTAGACGCCCGCAGCGATTTCGGC
CCGCTCAGCATTCCGGCCGGACAGTACATGATGCTAGGCGACAACCGCGACAACAGCGCGGACTCGCGCTACATCGGCCT
GGTGCCGCGCGAGCTGCTGATCGGCCGCGCCGTCGGCGTGATCGCCTCGGCCGACATCGCCGGCGGCTGGATGCCGCGCT
GGGAGCGCTTCGCCAGCGGCTTTCACTAA

Upstream 100 bases:

>100_bases
CGGTGCTGGAGGGCGGCTACGACCTGTCGGCGCTGGGCCGCAGCGTCGCCGCCCACATCCGCGTGCTGTCCGACGGCTGA
CCTAACGACTAGGAATCGAT

Downstream 100 bases:

>100_bases
AAGAGACACGCCAAGCAAGCAAAAAGCCGCCCCACGGGGCGGCTTTTCATGTTCGGGGCTGCGCGTCAGCCGGCATCGCC
CTTGTTGCCTATCTTGTCTT

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKNWLQNNRGFLVFLLLFGLFRTAVADWNPIPSGSMRPTLLEGDVVLVNRLAYDLKLPLTNVVLQQTGEPQRGDIVTFYS
PKDGKHLIKRLVAVPGDTVEMRRERLIINGRAADYQALQQVTETVSDHVALPALRLRESGALPAHRVQWLAGVDARSDFG
PLSIPAGQYMMLGDNRDNSADSRYIGLVPRELLIGRAVGVIASADIAGGWMPRWERFASGFH

Sequences:

>Translated_222_residues
MKNWLQNNRGFLVFLLLFGLFRTAVADWNPIPSGSMRPTLLEGDVVLVNRLAYDLKLPLTNVVLQQTGEPQRGDIVTFYS
PKDGKHLIKRLVAVPGDTVEMRRERLIINGRAADYQALQQVTETVSDHVALPALRLRESGALPAHRVQWLAGVDARSDFG
PLSIPAGQYMMLGDNRDNSADSRYIGLVPRELLIGRAVGVIASADIAGGWMPRWERFASGFH
>Mature_222_residues
MKNWLQNNRGFLVFLLLFGLFRTAVADWNPIPSGSMRPTLLEGDVVLVNRLAYDLKLPLTNVVLQQTGEPQRGDIVTFYS
PKDGKHLIKRLVAVPGDTVEMRRERLIINGRAADYQALQQVTETVSDHVALPALRLRESGALPAHRVQWLAGVDARSDFG
PLSIPAGQYMMLGDNRDNSADSRYIGLVPRELLIGRAVGVIASADIAGGWMPRWERFASGFH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=265, Percent_Identity=33.2075471698113, Blast_Score=121, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 24643; Mature: 24643

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWLQNNRGFLVFLLLFGLFRTAVADWNPIPSGSMRPTLLEGDVVLVNRLAYDLKLPLT
CCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEHHHHHHCCCHH
NVVLQQTGEPQRGDIVTFYSPKDGKHLIKRLVAVPGDTVEMRRERLIINGRAADYQALQQ
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHEEEECCCCHHHHHHHH
VTETVSDHVALPALRLRESGALPAHRVQWLAGVDARSDFGPLSIPAGQYMMLGDNRDNSA
HHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DSRYIGLVPRELLIGRAVGVIASADIAGGWMPRWERFASGFH
CCCEEECCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKNWLQNNRGFLVFLLLFGLFRTAVADWNPIPSGSMRPTLLEGDVVLVNRLAYDLKLPLT
CCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEHHHHHHCCCHH
NVVLQQTGEPQRGDIVTFYSPKDGKHLIKRLVAVPGDTVEMRRERLIINGRAADYQALQQ
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHEEEECCCCHHHHHHHH
VTETVSDHVALPALRLRESGALPAHRVQWLAGVDARSDFGPLSIPAGQYMMLGDNRDNSA
HHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DSRYIGLVPRELLIGRAVGVIASADIAGGWMPRWERFASGFH
CCCEEECCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6311837; 9278503; 2202591; 1618816; 1546969; 8394311; 2551889; 9823901 [H]