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The map label for this gene is ogt [H]

Identifier: 34499126

GI number: 34499126

Start: 3973018

End: 3973482

Strand: Reverse

Name: ogt [H]

Synonym: CV_3671

Alternate gene names: 34499126

Gene position: 3973482-3973018 (Counterclockwise)

Preceding gene: 34499127

Following gene: 34499125

Centisome position: 83.63

GC content: 70.97

Gene sequence:

>465_bases
ATGGACTATCCGGTGGTGATCGCCGCGCCGTTCGGCCGGCTGGGCGTGCGCTGCGAAGCCGGCGAGTTGCGCGAGCTGCG
CTTCCTGCCGGCGGGCATGGCCTTGCGGCCGCCGGCGCCGGGCAGCCTGGAGGCCGAGGTGGCCAGGCAGCTGGACGCTT
ATTACGCCGATCCGCGCCATGTGTTCACCGTGCCGCGGCGCTTGCTCGGCACGCCTTACCAGCTGCGGGTGTGGCAGCGG
ATTGCGGCGATACCCTGCGGCGAGACCCGCCGCTACCAGGACCTGTCCCGCGAGCTGTCATCGTCGCCGCGGGCTGTCGG
CGGCGCCTGCGGCCGCAACCCCATCCCCGTCATCGTGCCCTGCCACCGGGTAGTGGCCAGCGCCGGCCTCGGCGGCTTCA
ACCAGTCCACCGGCGACGAAACGCTGGACATCAAGAAATGGCTGCTGCGGCATGAGCTCGGATAA

Upstream 100 bases:

>100_bases
AAAGCTGCCGGCCCGCAAGCAGGGTTAATCCTGCCGGCGATCGCCGCTATCATCAAAGCGCAAGGCCTGGCCTTGCGCTT
TTTTCATTTGGGGAGGGGGA

Downstream 100 bases:

>100_bases
GGACAGCATCGACGCCTTCCTCGACCAGCTGTGGCTGGAGGACGGCCTGTCGCAGAACACGCTGGCCGCCTATCGCCGCG
ACCTGTCCAAGCTGGCGGCC

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MDYPVVIAAPFGRLGVRCEAGELRELRFLPAGMALRPPAPGSLEAEVARQLDAYYADPRHVFTVPRRLLGTPYQLRVWQR
IAAIPCGETRRYQDLSRELSSSPRAVGGACGRNPIPVIVPCHRVVASAGLGGFNQSTGDETLDIKKWLLRHELG

Sequences:

>Translated_154_residues
MDYPVVIAAPFGRLGVRCEAGELRELRFLPAGMALRPPAPGSLEAEVARQLDAYYADPRHVFTVPRRLLGTPYQLRVWQR
IAAIPCGETRRYQDLSRELSSSPRAVGGACGRNPIPVIVPCHRVVASAGLGGFNQSTGDETLDIKKWLLRHELG
>Mature_154_residues
MDYPVVIAAPFGRLGVRCEAGELRELRFLPAGMALRPPAPGSLEAEVARQLDAYYADPRHVFTVPRRLLGTPYQLRVWQR
IAAIPCGETRRYQDLSRELSSSPRAVGGACGRNPIPVIVPCHRVVASAGLGGFNQSTGDETLDIKKWLLRHELG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=169, Percent_Identity=34.3195266272189, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787596, Length=110, Percent_Identity=38.1818181818182, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788542, Length=75, Percent_Identity=44, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=66, Percent_Identity=48.4848484848485, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533070, Length=66, Percent_Identity=48.4848484848485, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16931; Mature: 16931

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYPVVIAAPFGRLGVRCEAGELRELRFLPAGMALRPPAPGSLEAEVARQLDAYYADPRH
CCCCEEEECCCCCCCCEECCCCCHHEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCHH
VFTVPRRLLGTPYQLRVWQRIAAIPCGETRRYQDLSRELSSSPRAVGGACGRNPIPVIVP
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEH
CHRVVASAGLGGFNQSTGDETLDIKKWLLRHELG
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYPVVIAAPFGRLGVRCEAGELRELRFLPAGMALRPPAPGSLEAEVARQLDAYYADPRH
CCCCEEEECCCCCCCCEECCCCCHHEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCHH
VFTVPRRLLGTPYQLRVWQRIAAIPCGETRRYQDLSRELSSSPRAVGGACGRNPIPVIVP
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEH
CHRVVASAGLGGFNQSTGDETLDIKKWLLRHELG
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]