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The map label for this gene is cysB1 [H]

Identifier: 34499030

GI number: 34499030

Start: 3882807

End: 3883751

Strand: Direct

Name: cysB1 [H]

Synonym: CV_3575

Alternate gene names: 34499030

Gene position: 3882807-3883751 (Clockwise)

Preceding gene: 34499021

Following gene: 34499033

Centisome position: 81.72

GC content: 65.29

Gene sequence:

>945_bases
ATGAAGCTACAACAACTCCGCTACCTGGTCGAGGTGGCCAAGCAGGGCCTCAACGTTTCCGAGGCCGCCGAGAAGCTGCA
TACCTCGCAGCCGGGCATTTCCAAGCAGATCCGCCTGCTGGAGGACGAACTGGGCATCCAGGTGTTCATCCGCAACGGCA
AGCGGGTGGTGTCGGTATCCGAGCCGGGCAAGGAAGTGCTGCGCATCTCCGAGCGCATCCTGCGCGAGGCGCAGAACCTG
AAACGGGTCGGCGACGAGTTCTCCCGCGAGTCTGAAGGCGCGCTGACCATCGCCACCACCCACACCCAGGCCCGCTACGC
GCTGCCGCAGGCGATCGCCGAATTCGTGCGCCGCTACCCCAAGGTGCGGCTGTCGCTGAAGCAGGGCAGCCCGACGCAGA
TCTGCGACATGGTGGTGTCCGGCGAGGCCGATCTCGCCATCGCCACCGAGGGCATCGCGCTGTACAAGGAGCTGGCGACG
CTGCCTTGCTACGAATGGAACCGCTCGCTGGTGGTGCCCAAGGGCCACCCGCTGGCCGCGCTGGAACGCGAAATCACCCT
GGCGGACATCGCTCAATACCCGATCGTCACCTACGACTTCGCCTTCGCCGGCCGCTCCAAGATCAACAAGTCGTTCGCCG
AACAGGGCCTGACGCCCAACGTGGTGCTGACCGCGATCGACACCGACGTGATCAAGACCTATGTCTCGCTGGGGCTGGGC
ATCGGCATCATCGCCAGCATGGCCTTCGAGCCGGAGCGCGACCACAACCTGACGGCGATCGACGCCGGCCACCTGTTCGA
GCCGTCCACCACCAAGATCGGCATCCGCAAGGACGCCTACCTGCGCGGCTTCGCCTACACCTTCATCGAGCTGTTCGCGC
CGCATCTGCACCGCCGCGAGGTAGACTCGGCGCTGCTGGCGCACGATGTCGACGACGAGGAATGA

Upstream 100 bases:

>100_bases
GGATGGTTCGAGCGTATCCTAACCAAAGCGCTTATAAACCTAAAAGAATATTGTGTTAGTATTTAATGACTACAGGTTGT
ATAGAGGACAGACTGCCAAG

Downstream 100 bases:

>100_bases
GCGCCCGCCGCGGATATCGTGATTCGGCGGGAACCTGGAGGGCCCGGCAAAGCCGGGCCTTTTCACTCTTGCAGCCCGTT
CCGCAGCCTGCCGTTGAAAT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKLQQLRYLVEVAKQGLNVSEAAEKLHTSQPGISKQIRLLEDELGIQVFIRNGKRVVSVSEPGKEVLRISERILREAQNL
KRVGDEFSRESEGALTIATTHTQARYALPQAIAEFVRRYPKVRLSLKQGSPTQICDMVVSGEADLAIATEGIALYKELAT
LPCYEWNRSLVVPKGHPLAALEREITLADIAQYPIVTYDFAFAGRSKINKSFAEQGLTPNVVLTAIDTDVIKTYVSLGLG
IGIIASMAFEPERDHNLTAIDAGHLFEPSTTKIGIRKDAYLRGFAYTFIELFAPHLHRREVDSALLAHDVDDEE

Sequences:

>Translated_314_residues
MKLQQLRYLVEVAKQGLNVSEAAEKLHTSQPGISKQIRLLEDELGIQVFIRNGKRVVSVSEPGKEVLRISERILREAQNL
KRVGDEFSRESEGALTIATTHTQARYALPQAIAEFVRRYPKVRLSLKQGSPTQICDMVVSGEADLAIATEGIALYKELAT
LPCYEWNRSLVVPKGHPLAALEREITLADIAQYPIVTYDFAFAGRSKINKSFAEQGLTPNVVLTAIDTDVIKTYVSLGLG
IGIIASMAFEPERDHNLTAIDAGHLFEPSTTKIGIRKDAYLRGFAYTFIELFAPHLHRREVDSALLAHDVDDEE
>Mature_314_residues
MKLQQLRYLVEVAKQGLNVSEAAEKLHTSQPGISKQIRLLEDELGIQVFIRNGKRVVSVSEPGKEVLRISERILREAQNL
KRVGDEFSRESEGALTIATTHTQARYALPQAIAEFVRRYPKVRLSLKQGSPTQICDMVVSGEADLAIATEGIALYKELAT
LPCYEWNRSLVVPKGHPLAALEREITLADIAQYPIVTYDFAFAGRSKINKSFAEQGLTPNVVLTAIDTDVIKTYVSLGLG
IGIIASMAFEPERDHNLTAIDAGHLFEPSTTKIGIRKDAYLRGFAYTFIELFAPHLHRREVDSALLAHDVDDEE

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=56.0655737704918, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788296, Length=291, Percent_Identity=46.0481099656357, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=34.1463414634146, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790399, Length=259, Percent_Identity=27.4131274131274, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=26.7558528428094, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367136, Length=243, Percent_Identity=25.1028806584362, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788481, Length=251, Percent_Identity=26.6932270916335, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=26.9076305220884, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=25.0980392156863, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787601, Length=182, Percent_Identity=29.1208791208791, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=265, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789204, Length=230, Percent_Identity=25.2173913043478, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=301, Percent_Identity=23.5880398671096, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081904, Length=220, Percent_Identity=28.1818181818182, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34912; Mature: 34912

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYLVEVAKQGLNVSEAAEKLHTSQPGISKQIRLLEDELGIQVFIRNGKRVVSVS
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEEC
EPGKEVLRISERILREAQNLKRVGDEFSRESEGALTIATTHTQARYALPQAIAEFVRRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
KVRLSLKQGSPTQICDMVVSGEADLAIATEGIALYKELATLPCYEWNRSLVVPKGHPLAA
CEEEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHH
LEREITLADIAQYPIVTYDFAFAGRSKINKSFAEQGLTPNVVLTAIDTDVIKTYVSLGLG
HHHHHHHHHHHCCCEEEEEEHHCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
IGIIASMAFEPERDHNLTAIDAGHLFEPSTTKIGIRKDAYLRGFAYTFIELFAPHLHRRE
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VDSALLAHDVDDEE
HHHHHHHCCCCCCC
>Mature Secondary Structure
MKLQQLRYLVEVAKQGLNVSEAAEKLHTSQPGISKQIRLLEDELGIQVFIRNGKRVVSVS
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEEC
EPGKEVLRISERILREAQNLKRVGDEFSRESEGALTIATTHTQARYALPQAIAEFVRRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
KVRLSLKQGSPTQICDMVVSGEADLAIATEGIALYKELATLPCYEWNRSLVVPKGHPLAA
CEEEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHH
LEREITLADIAQYPIVTYDFAFAGRSKINKSFAEQGLTPNVVLTAIDTDVIKTYVSLGLG
HHHHHHHHHHHCCCEEEEEEHHCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
IGIIASMAFEPERDHNLTAIDAGHLFEPSTTKIGIRKDAYLRGFAYTFIELFAPHLHRRE
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VDSALLAHDVDDEE
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]