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The map label for this gene is cysH [H]

Identifier: 34499029

GI number: 34499029

Start: 3881990

End: 3882694

Strand: Reverse

Name: cysH [H]

Synonym: CV_3574

Alternate gene names: 34499029

Gene position: 3882694-3881990 (Counterclockwise)

Preceding gene: 34499031

Following gene: 34499028

Centisome position: 81.72

GC content: 65.96

Gene sequence:

>705_bases
ATGAGCCTGGAGTCCAAACTCGCCGCCACCACGGCCCTGCTGCAAACCATCGCCGCCGAACATCCCGACGCGGTGCTGGC
CAACAGCTACGGCGCCGAAGACATGGTGCTGACCGACCTGATCGCCCGTCTCGGCCTGCCGCTGGAAAGCTTCAGCCTGG
ACACCGGCCGCCTGCCGGCCGAAACCTATGAGTTGATGCAGCAAGTCGCCGAGCGTTATCCAGGCCATCCGGTCAGGATG
TACTTCCCCGACACCGCCGCCACCGAGCAGTACGTCAACTTCCACGGCATCAACGGCTTCTATCGGAGCGTCGAGTTGCG
CAAGTCCTGTTGCGCGGTTCGCAAGCTGGAGCCGCTGAAGCGCGCGCTGGCCGGCCGATCGGCCTGGATCACCGGCCTGC
GCCGCCAGCAGTCGCCGACGCGCCAGGACCTGGCCGACAGGGAGTACGACGCCGACAACGGGCTCGTGAAATTCAGCCCG
CTGCTGGAATGGACGGAAGCCGAAGTGTGGCAGTACCTGAAGGAAAACCAGGTGCCGTACAACGCGCTGCACGATCGGCA
CTATCCGTCGATAGGCTGCGCGCCCTGTACCCGCGCCATCAGCGTGGGCGAAGACGTGCGCGCCGGCCGCTGGTGGTGGG
AAAACCCGGAAACCAAGGAGTGCGGGCTGCACGCGAAGTCCAGTCCGCTGCAGCGCCCCGTCTGA

Upstream 100 bases:

>100_bases
TCCTCTATACAACCTGTAGTCATTAAATACTAACACAATATTCTTTTAGGTTTATAAGCGCTTTGGTTAGGATACGCTCG
AACCATCCCCGGAGTGCTTG

Downstream 100 bases:

>100_bases
AACGCCGGCCCGCCGATGCGGGCATCCCCGTTGGAATCCAAACATGTATCGATACGATGAAGTAGACCATCGGCTGGTGC
GCGAGCGCGTGGTCCAGTTT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSLESKLAATTALLQTIAAEHPDAVLANSYGAEDMVLTDLIARLGLPLESFSLDTGRLPAETYELMQQVAERYPGHPVRM
YFPDTAATEQYVNFHGINGFYRSVELRKSCCAVRKLEPLKRALAGRSAWITGLRRQQSPTRQDLADREYDADNGLVKFSP
LLEWTEAEVWQYLKENQVPYNALHDRHYPSIGCAPCTRAISVGEDVRAGRWWWENPETKECGLHAKSSPLQRPV

Sequences:

>Translated_234_residues
MSLESKLAATTALLQTIAAEHPDAVLANSYGAEDMVLTDLIARLGLPLESFSLDTGRLPAETYELMQQVAERYPGHPVRM
YFPDTAATEQYVNFHGINGFYRSVELRKSCCAVRKLEPLKRALAGRSAWITGLRRQQSPTRQDLADREYDADNGLVKFSP
LLEWTEAEVWQYLKENQVPYNALHDRHYPSIGCAPCTRAISVGEDVRAGRWWWENPETKECGLHAKSSPLQRPV
>Mature_233_residues
SLESKLAATTALLQTIAAEHPDAVLANSYGAEDMVLTDLIARLGLPLESFSLDTGRLPAETYELMQQVAERYPGHPVRMY
FPDTAATEQYVNFHGINGFYRSVELRKSCCAVRKLEPLKRALAGRSAWITGLRRQQSPTRQDLADREYDADNGLVKFSPL
LEWTEAEVWQYLKENQVPYNALHDRHYPSIGCAPCTRAISVGEDVRAGRWWWENPETKECGLHAKSSPLQRPV

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=230, Percent_Identity=27.8260869565217, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325425, Length=215, Percent_Identity=31.6279069767442, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 26421; Mature: 26290

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLESKLAATTALLQTIAAEHPDAVLANSYGAEDMVLTDLIARLGLPLESFSLDTGRLPA
CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCH
ETYELMQQVAERYPGHPVRMYFPDTAATEQYVNFHGINGFYRSVELRKSCCAVRKLEPLK
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RALAGRSAWITGLRRQQSPTRQDLADREYDADNGLVKFSPLLEWTEAEVWQYLKENQVPY
HHHCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCH
NALHDRHYPSIGCAPCTRAISVGEDVRAGRWWWENPETKECGLHAKSSPLQRPV
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLESKLAATTALLQTIAAEHPDAVLANSYGAEDMVLTDLIARLGLPLESFSLDTGRLPA
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCH
ETYELMQQVAERYPGHPVRMYFPDTAATEQYVNFHGINGFYRSVELRKSCCAVRKLEPLK
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RALAGRSAWITGLRRQQSPTRQDLADREYDADNGLVKFSPLLEWTEAEVWQYLKENQVPY
HHHCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCH
NALHDRHYPSIGCAPCTRAISVGEDVRAGRWWWENPETKECGLHAKSSPLQRPV
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]