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The map label for this gene is ispG

Identifier: 34498993

GI number: 34498993

Start: 3846458

End: 3847741

Strand: Reverse

Name: ispG

Synonym: CV_3538

Alternate gene names: 34498993

Gene position: 3847741-3846458 (Counterclockwise)

Preceding gene: 34498994

Following gene: 34498992

Centisome position: 80.99

GC content: 67.21

Gene sequence:

>1284_bases
ATGGATAGCGTGAACATCGCGCGCCGCCCCACCCGCCTGGTGCGGGTGGGGCATGTTTTGGTGGGATCGGACGCGCCGGT
GATGGTGCAGTCCATGACCAATACCGACACCGCCGACGCGGCGGGCACGGCCGAACAGGTATACCAGCTGGCGCAGGCCG
GCTCCGAGGTGGTGCGCATCACCGTCAACTCGCCGGAAGCCGCGGCGCGGGTGGCGGAGATCCGCCAGCGGCTGGACGAC
CTGGGCTGCGACGTGCCCCTGGTCGGCGATTTCCACTTCAACGGCGATCGGCTGCTGAAGGAGTTCCCGGACTGCGCGCG
GGCGCTGGCCAAGTACCGGATCAATCCGGGCAACGTCGGCAAGGGCGCCAAGGGCGACGACAAGTTCGCCTTCATGATCC
GCACCGCGATGGAGCACGACAAGGCCGTCCGCATCGGCGTCAACTGGGGCAGCCTGGACCAGGCGCTGCTGGCGCGGATG
ATGGACGCCAACAACCGCGCAGCCAACCCGCTGCCGCTGCCCAAGCTGATGCAGGAGGCGCTGATCGTGTCGGCGCTGGA
GTCGGCGGAGAAAGCGGTGGAAATCGGCCTGTCGCCCGACAACATCATCCTGTCGTGCAAGGTCAGCAATGTGCAGGACC
TGATCAGCGTCTACCGCGAGCTCGGCGGTCGCTGCGACTACCCGCTGCACCTGGGCCTGACCGAGGCCGGCATGGGCAGC
AAGGGCATCGTCGCCTCCAGCGCCGCGCTGGCCGTGTTGTTGCAGGAAGGCATAGGCGACACCATCCGCATCTCGCTGAC
GCCGCAGCCGGGCGAGGCGCGCACCAAGGAGGTGGTGGTCGCGCAGGAGTTGCTGCAGACCATGGGCCTGCGCAGCTTCA
CGCCGCTGGTCACCGCCTGTCCGGGCTGCGGCCGCACCACCAGCACCTTCTTCCAGGAGCTGGCCGATCATATCCAGAGC
TATCTGCGCGAGCGGATGCCGGTGTGGCGGCTGCAGTATCCGGGCGTCGAAGACATGAAGGTGGCGGTGATGGGCTGCGT
GGTCAACGGTCCGGGCGAGTCCAAGCTGGCCGACATCGGCATTTCGTTGCCTGGCACCGGCGAAGTGCCGGTGGCGCCGG
TCTACGTGGACGGCCAGAAGGATGTGACGCTGAAGGGCGATAACATTCCGGCGGAATTCACCGCCATCGTCGACAACTAT
GTGAAAACGCGTTATGGCGAGGGCGGGGCCAAGCGCCGCGAGGTCGCCAGCCGCACGATTCCGATCCGGCCGGTGAAGGC
CTGA

Upstream 100 bases:

>100_bases
AGCCAGGTGGAGCTGAGCTACAACGGCAAGCCGGTGGACCTGGCCGACAAGATCAAGGGCACCACCGCCAAGATTCAACT
CAAGTGATGCAGGGTAGCGA

Downstream 100 bases:

>100_bases
CGGAAACAGAATCCAGCTGATACAAGAAGATAGCAATGGCTCAGAAATACCAAGCGGTCAAAGGCATGAACGATGTGCTG
CCGGCCGAATCCTACCAGTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDSVNIARRPTRLVRVGHVLVGSDAPVMVQSMTNTDTADAAGTAEQVYQLAQAGSEVVRITVNSPEAAARVAEIRQRLDD
LGCDVPLVGDFHFNGDRLLKEFPDCARALAKYRINPGNVGKGAKGDDKFAFMIRTAMEHDKAVRIGVNWGSLDQALLARM
MDANNRAANPLPLPKLMQEALIVSALESAEKAVEIGLSPDNIILSCKVSNVQDLISVYRELGGRCDYPLHLGLTEAGMGS
KGIVASSAALAVLLQEGIGDTIRISLTPQPGEARTKEVVVAQELLQTMGLRSFTPLVTACPGCGRTTSTFFQELADHIQS
YLRERMPVWRLQYPGVEDMKVAVMGCVVNGPGESKLADIGISLPGTGEVPVAPVYVDGQKDVTLKGDNIPAEFTAIVDNY
VKTRYGEGGAKRREVASRTIPIRPVKA

Sequences:

>Translated_427_residues
MDSVNIARRPTRLVRVGHVLVGSDAPVMVQSMTNTDTADAAGTAEQVYQLAQAGSEVVRITVNSPEAAARVAEIRQRLDD
LGCDVPLVGDFHFNGDRLLKEFPDCARALAKYRINPGNVGKGAKGDDKFAFMIRTAMEHDKAVRIGVNWGSLDQALLARM
MDANNRAANPLPLPKLMQEALIVSALESAEKAVEIGLSPDNIILSCKVSNVQDLISVYRELGGRCDYPLHLGLTEAGMGS
KGIVASSAALAVLLQEGIGDTIRISLTPQPGEARTKEVVVAQELLQTMGLRSFTPLVTACPGCGRTTSTFFQELADHIQS
YLRERMPVWRLQYPGVEDMKVAVMGCVVNGPGESKLADIGISLPGTGEVPVAPVYVDGQKDVTLKGDNIPAEFTAIVDNY
VKTRYGEGGAKRREVASRTIPIRPVKA
>Mature_427_residues
MDSVNIARRPTRLVRVGHVLVGSDAPVMVQSMTNTDTADAAGTAEQVYQLAQAGSEVVRITVNSPEAAARVAEIRQRLDD
LGCDVPLVGDFHFNGDRLLKEFPDCARALAKYRINPGNVGKGAKGDDKFAFMIRTAMEHDKAVRIGVNWGSLDQALLARM
MDANNRAANPLPLPKLMQEALIVSALESAEKAVEIGLSPDNIILSCKVSNVQDLISVYRELGGRCDYPLHLGLTEAGMGS
KGIVASSAALAVLLQEGIGDTIRISLTPQPGEARTKEVVVAQELLQTMGLRSFTPLVTACPGCGRTTSTFFQELADHIQS
YLRERMPVWRLQYPGVEDMKVAVMGCVVNGPGESKLADIGISLPGTGEVPVAPVYVDGQKDVTLKGDNIPAEFTAIVDNY
VKTRYGEGGAKRREVASRTIPIRPVKA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=400, Percent_Identity=33.25, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CHRVO (Q7NS88)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903208.1
- ProteinModelPortal:   Q7NS88
- GeneID:   2547539
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3538
- NMPDR:   fig|243365.1.peg.3538
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q7NS88
- ProtClustDB:   PRK00366
- BioCyc:   CVIO243365:CV_3538-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 45876; Mature: 45876

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSVNIARRPTRLVRVGHVLVGSDAPVMVQSMTNTDTADAAGTAEQVYQLAQAGSEVVRI
CCCCCCCCCCHHHHEECEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEE
TVNSPEAAARVAEIRQRLDDLGCDVPLVGDFHFNGDRLLKEFPDCARALAKYRINPGNVG
EECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
KGAKGDDKFAFMIRTAMEHDKAVRIGVNWGSLDQALLARMMDANNRAANPLPLPKLMQEA
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LIVSALESAEKAVEIGLSPDNIILSCKVSNVQDLISVYRELGGRCDYPLHLGLTEAGMGS
HHHHHHHHHHHHEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
KGIVASSAALAVLLQEGIGDTIRISLTPQPGEARTKEVVVAQELLQTMGLRSFTPLVTAC
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCC
PGCGRTTSTFFQELADHIQSYLRERMPVWRLQYPGVEDMKVAVMGCVVNGPGESKLADIG
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEC
ISLPGTGEVPVAPVYVDGQKDVTLKGDNIPAEFTAIVDNYVKTRYGEGGAKRREVASRTI
EECCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
PIRPVKA
CCCCCCC
>Mature Secondary Structure
MDSVNIARRPTRLVRVGHVLVGSDAPVMVQSMTNTDTADAAGTAEQVYQLAQAGSEVVRI
CCCCCCCCCCHHHHEECEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEE
TVNSPEAAARVAEIRQRLDDLGCDVPLVGDFHFNGDRLLKEFPDCARALAKYRINPGNVG
EECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
KGAKGDDKFAFMIRTAMEHDKAVRIGVNWGSLDQALLARMMDANNRAANPLPLPKLMQEA
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LIVSALESAEKAVEIGLSPDNIILSCKVSNVQDLISVYRELGGRCDYPLHLGLTEAGMGS
HHHHHHHHHHHHEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
KGIVASSAALAVLLQEGIGDTIRISLTPQPGEARTKEVVVAQELLQTMGLRSFTPLVTAC
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCC
PGCGRTTSTFFQELADHIQSYLRERMPVWRLQYPGVEDMKVAVMGCVVNGPGESKLADIG
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEC
ISLPGTGEVPVAPVYVDGQKDVTLKGDNIPAEFTAIVDNYVKTRYGEGGAKRREVASRTI
EECCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
PIRPVKA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782