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The map label for this gene is hisS

Identifier: 34498992

GI number: 34498992

Start: 3845151

End: 3846422

Strand: Reverse

Name: hisS

Synonym: CV_3537

Alternate gene names: 34498992

Gene position: 3846422-3845151 (Counterclockwise)

Preceding gene: 34498993

Following gene: 34498991

Centisome position: 80.96

GC content: 65.96

Gene sequence:

>1272_bases
ATGGCTCAGAAATACCAAGCGGTCAAAGGCATGAACGATGTGCTGCCGGCCGAATCCTACCAGTGGGAATACTTTGAAGA
GGCGCTGCGCAAGCTGCTGGCCGACTACGGCTACCAGAACATCCGCACCCCCATCGTCGAAGGCACGCCGCTGTTCGTGC
GTTCCATCGGCGAAGTGACCGACATCGTCGAGAAGGAAATGTACACCTTCGTCGACAGCCTGAACGGCGACAGCCTGACG
CTGCGCCCGGAAGGCACCGCCGGCACGCTGCGCGCGGTGGTCGAGCATAATCTGCTGTACAACGCCACGCCCAAGCTGTG
GTACATGGGCCCGATGTACCGCCACGAGCGCCCGCAGAAGGGCCGCTACCGCCAATTCCACCAGGTGGGCGTGGAGGCGC
TGGGCCTGGCCGGTCCGGACATCGACGCGGAAATCATCGCGATGACCGCCGACCTCTGGCGCCGCCTTGGCATCAGCCAG
TATGTGCGGCTGGAGATCAACTCGCTGGGCAACGCCGAAGAGCGCGCCGCCCACCGCGAGGCGCTGATCGCCTACCTGGA
ACGCCACGTCGACATCCTGGACGAGGACGGCAAGCGCCGGATGCACACCAACCCGCTGCGCGTGCTGGACACCAAGAACC
CGGCCTTGCAGGAAATGGCCAACGCCGCGCCCAAGCTGTCCGACTACCTGGGCGAGGAGTCGCGCGCCCATTACGAGGGC
TGGAAGGCGATGATCGCCGCGCTGGGCATCGAGTACATCGAGAATCCGCGCCTGGTGCGCGGCCTCGACTACTACAACCG
CTCGGTGTTCGAGTGGGTGACCTCCGAGCTGGGCGCCCAGGGCACCATCTGCGCCGGCGGCCGTTACGACGGCCTGATCG
AGCAGCTGGGCGGCAAGGCCGCGTCGGGCATCGGCTTCGGCATGGGCATGGAGCGCGTGCTGCTGTTGCTGCAGGACAAG
GGCCTGCTGCCGGCGCAGCGCAGCGTCGACGTGTTTCTGGTCAACCAGGGCGAGGGCGCCGGCCTGTACTCGATGAAACT
GGCTCAAACCCTGCGCGCCGCCGGCTATTCGGTGGTGCAGCACCTGGGCGAGGCCAGCTTCAAGTCGCAGATGAAAAAGG
CCGACGGCAGCGGCGCGGAATTCGCGCTGATCGTCGGCGAAAACGAAATCCAGGCCGGTCAGGTGGTGGTGAAGGCGCTG
CGCGCCGACGTCGCCCAGCAGACCGTGGCGGCCGATGCGGTTCTGGCCACCCTCGCCACTCTGAAAGCTTGA

Upstream 100 bases:

>100_bases
AGGGCGGGGCCAAGCGCCGCGAGGTCGCCAGCCGCACGATTCCGATCCGGCCGGTGAAGGCCTGACGGAAACAGAATCCA
GCTGATACAAGAAGATAGCA

Downstream 100 bases:

>100_bases
TAGGAAGGGGGAACGCGATGGCGTTCGACTTGCAGGAACAGGAACAGATCGATTCTCTGAAGGCATTCTGGCAGCAGTGG
GGCAAGCTGATCGGCGGCGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAQKYQAVKGMNDVLPAESYQWEYFEEALRKLLADYGYQNIRTPIVEGTPLFVRSIGEVTDIVEKEMYTFVDSLNGDSLT
LRPEGTAGTLRAVVEHNLLYNATPKLWYMGPMYRHERPQKGRYRQFHQVGVEALGLAGPDIDAEIIAMTADLWRRLGISQ
YVRLEINSLGNAEERAAHREALIAYLERHVDILDEDGKRRMHTNPLRVLDTKNPALQEMANAAPKLSDYLGEESRAHYEG
WKAMIAALGIEYIENPRLVRGLDYYNRSVFEWVTSELGAQGTICAGGRYDGLIEQLGGKAASGIGFGMGMERVLLLLQDK
GLLPAQRSVDVFLVNQGEGAGLYSMKLAQTLRAAGYSVVQHLGEASFKSQMKKADGSGAEFALIVGENEIQAGQVVVKAL
RADVAQQTVAADAVLATLATLKA

Sequences:

>Translated_423_residues
MAQKYQAVKGMNDVLPAESYQWEYFEEALRKLLADYGYQNIRTPIVEGTPLFVRSIGEVTDIVEKEMYTFVDSLNGDSLT
LRPEGTAGTLRAVVEHNLLYNATPKLWYMGPMYRHERPQKGRYRQFHQVGVEALGLAGPDIDAEIIAMTADLWRRLGISQ
YVRLEINSLGNAEERAAHREALIAYLERHVDILDEDGKRRMHTNPLRVLDTKNPALQEMANAAPKLSDYLGEESRAHYEG
WKAMIAALGIEYIENPRLVRGLDYYNRSVFEWVTSELGAQGTICAGGRYDGLIEQLGGKAASGIGFGMGMERVLLLLQDK
GLLPAQRSVDVFLVNQGEGAGLYSMKLAQTLRAAGYSVVQHLGEASFKSQMKKADGSGAEFALIVGENEIQAGQVVVKAL
RADVAQQTVAADAVLATLATLKA
>Mature_422_residues
AQKYQAVKGMNDVLPAESYQWEYFEEALRKLLADYGYQNIRTPIVEGTPLFVRSIGEVTDIVEKEMYTFVDSLNGDSLTL
RPEGTAGTLRAVVEHNLLYNATPKLWYMGPMYRHERPQKGRYRQFHQVGVEALGLAGPDIDAEIIAMTADLWRRLGISQY
VRLEINSLGNAEERAAHREALIAYLERHVDILDEDGKRRMHTNPLRVLDTKNPALQEMANAAPKLSDYLGEESRAHYEGW
KAMIAALGIEYIENPRLVRGLDYYNRSVFEWVTSELGAQGTICAGGRYDGLIEQLGGKAASGIGFGMGMERVLLLLQDKG
LLPAQRSVDVFLVNQGEGAGLYSMKLAQTLRAAGYSVVQHLGEASFKSQMKKADGSGAEFALIVGENEIQAGQVVVKALR
ADVAQQTVAADAVLATLATLKA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=57.7830188679245, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CHRVO (Q7NS89)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903207.1
- ProteinModelPortal:   Q7NS89
- SMR:   Q7NS89
- GeneID:   2547538
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3537
- NMPDR:   fig|243365.1.peg.3537
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q7NS89
- ProtClustDB:   PRK00037
- BioCyc:   CVIO243365:CV_3537-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46730; Mature: 46599

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKYQAVKGMNDVLPAESYQWEYFEEALRKLLADYGYQNIRTPIVEGTPLFVRSIGEVT
CCCHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DIVEKEMYTFVDSLNGDSLTLRPEGTAGTLRAVVEHNLLYNATPKLWYMGPMYRHERPQK
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCC
GRYRQFHQVGVEALGLAGPDIDAEIIAMTADLWRRLGISQYVRLEINSLGNAEERAAHRE
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
ALIAYLERHVDILDEDGKRRMHTNPLRVLDTKNPALQEMANAAPKLSDYLGEESRAHYEG
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHCCHHHHHHHH
WKAMIAALGIEYIENPRLVRGLDYYNRSVFEWVTSELGAQGTICAGGRYDGLIEQLGGKA
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
ASGIGFGMGMERVLLLLQDKGLLPAQRSVDVFLVNQGEGAGLYSMKLAQTLRAAGYSVVQ
CCCCCHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
HLGEASFKSQMKKADGSGAEFALIVGENEIQAGQVVVKALRADVAQQTVAADAVLATLAT
HHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKA
HCC
>Mature Secondary Structure 
AQKYQAVKGMNDVLPAESYQWEYFEEALRKLLADYGYQNIRTPIVEGTPLFVRSIGEVT
CCHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DIVEKEMYTFVDSLNGDSLTLRPEGTAGTLRAVVEHNLLYNATPKLWYMGPMYRHERPQK
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCC
GRYRQFHQVGVEALGLAGPDIDAEIIAMTADLWRRLGISQYVRLEINSLGNAEERAAHRE
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
ALIAYLERHVDILDEDGKRRMHTNPLRVLDTKNPALQEMANAAPKLSDYLGEESRAHYEG
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHCCHHHHHHHH
WKAMIAALGIEYIENPRLVRGLDYYNRSVFEWVTSELGAQGTICAGGRYDGLIEQLGGKA
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
ASGIGFGMGMERVLLLLQDKGLLPAQRSVDVFLVNQGEGAGLYSMKLAQTLRAAGYSVVQ
CCCCCHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
HLGEASFKSQMKKADGSGAEFALIVGENEIQAGQVVVKALRADVAQQTVAADAVLATLAT
HHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782