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The map label for this gene is cysB2 [H]

Identifier: 34498850

GI number: 34498850

Start: 3689164

End: 3690534

Strand: Reverse

Name: cysB2 [H]

Synonym: CV_3395

Alternate gene names: 34498850

Gene position: 3690534-3689164 (Counterclockwise)

Preceding gene: 34498851

Following gene: 34498849

Centisome position: 77.68

GC content: 66.3

Gene sequence:

>1371_bases
ATGCAGGACAATAGCGTCTTATCGCTGATCGGCAACACGCCATTGGTTCGCGTCAGCCATCTGGATACCGGGCCGTGCGA
GCTGTACCTGAAGCTGGAAAGCCACAATCCGGGCGGCTCCATCAAGGATCGGATCGCCTTGACCATGATCGAGGCGGCCG
AGCGGGACGGCAAGTTGAAACCGGGCGGGGCCATCATCGAGGCCACCGCCGGCAATACCGGGCTGGGTCTCGCGCTGGTG
GCCGCGCAGAAGGGCTACCCGCTGACGCTGGTGGTGCCGGACAAGATGAGCCGGGAGAAGATTTTGCATCTGCAGGCGCT
GGGCGCCCAGGTGGTGCTGACCCGCTCCGACGTCGGCAAGGGCCATCCGGAGTACTACCAGGACCTGGCGGCGCGCATCG
CCAGCGAGACGCCCGGCGCCTACTACATCGACCAGTTCAACAACCCGGCCAATCCGCTGGCGCATGAAACCACCACCGGG
CCGGAAATCTGGCGCCAGATGAACGGCGAGGTGGACGCGGTGGTGGTCGGCGTCGGCTCCAGCGGCACGCTGACCGGCCT
GACCCGCTTCTTCCGCGCCAAGGCGCCGGCCACCGCCTTCGTGCTGGCCGATCCGGTGGGGTCTGTGTTGGCCGATTATG
TCGAAACCGGCAGCTTCGGCCAGGCCGGCAGCTGGCTGATCGAGGGCATAGGCGAGGACTTCATCCCGCCGCAGACCGAT
CTGTCGATGGTGAAGCGCGCCTTCCGCGTCAGCGATCAGGAAAGCTTCGACACCGCCCAGCAACTGCTGCGCAGCGAGGG
CATTCTGGCCGGTTCGTCGTCCGGCGCGTTGCTTGCCGCCGCGTTGCGCTACTGCCGCGAGCAAACCGAGCCCAAGCGGG
TGGTGACGCTGGTGTGCGACAGCGGCAACAAGTACCTGTCCAAGATGTACAACCGCGACTGGCTGGTCGACCAGGGCATG
CTGCGCCTGCCCGAGGTGGGCGGACTGACCGATCTGATCGTCCGCCGCCACCAGGACGGCAGCACGGTGTCTTGCCATCC
GGACGAGCCGCTCAATGTGGCTTACCAGCGGATGCGCCTGCACGACGTGTCGCAGATGCCGGTGCTGGACGACGACAAGG
TGGTCGGCGTGCTGGACGAATGGGACCTGCTGCTGTCGGTGCACGGCGAGCCGGAAAACTTCCGCCAGACCGTGGCCACG
GCGATGAGCAGCCATGTGCGCACGCTGTCGCCGCAGGCCTCGTTGCAGGATCTGCTGCAGGTGTTCAACGACGGCCACAT
CGCGCTGATCGTCGACAAGGGCCGCTATCTGGGGCTGATCACCCAGGCCGACCTGCTGTCGTTCTGGCGCCGCCAGCCGC
GCGCCGCTTAA

Upstream 100 bases:

>100_bases
CTATATTCAGTAGGACTACTTCTCCTTTGTAGAAGATTCTGCAATTTTTATATAACAAAAAATTACTAAAAGTAATTTTA
AAATAGCCAGGAGTATTGAA

Downstream 100 bases:

>100_bases
GCCGATTTCCCGCCCTGGCCGATTGCGCCGGGGCGCATGATTTTGAGAGAGGATTGAAAACATGACCGATTTCCACACCC
TGGCCGTGACCGCCGGCGCG

Product: cystathionine beta-synthase

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MQDNSVLSLIGNTPLVRVSHLDTGPCELYLKLESHNPGGSIKDRIALTMIEAAERDGKLKPGGAIIEATAGNTGLGLALV
AAQKGYPLTLVVPDKMSREKILHLQALGAQVVLTRSDVGKGHPEYYQDLAARIASETPGAYYIDQFNNPANPLAHETTTG
PEIWRQMNGEVDAVVVGVGSSGTLTGLTRFFRAKAPATAFVLADPVGSVLADYVETGSFGQAGSWLIEGIGEDFIPPQTD
LSMVKRAFRVSDQESFDTAQQLLRSEGILAGSSSGALLAAALRYCREQTEPKRVVTLVCDSGNKYLSKMYNRDWLVDQGM
LRLPEVGGLTDLIVRRHQDGSTVSCHPDEPLNVAYQRMRLHDVSQMPVLDDDKVVGVLDEWDLLLSVHGEPENFRQTVAT
AMSSHVRTLSPQASLQDLLQVFNDGHIALIVDKGRYLGLITQADLLSFWRRQPRAA

Sequences:

>Translated_456_residues
MQDNSVLSLIGNTPLVRVSHLDTGPCELYLKLESHNPGGSIKDRIALTMIEAAERDGKLKPGGAIIEATAGNTGLGLALV
AAQKGYPLTLVVPDKMSREKILHLQALGAQVVLTRSDVGKGHPEYYQDLAARIASETPGAYYIDQFNNPANPLAHETTTG
PEIWRQMNGEVDAVVVGVGSSGTLTGLTRFFRAKAPATAFVLADPVGSVLADYVETGSFGQAGSWLIEGIGEDFIPPQTD
LSMVKRAFRVSDQESFDTAQQLLRSEGILAGSSSGALLAAALRYCREQTEPKRVVTLVCDSGNKYLSKMYNRDWLVDQGM
LRLPEVGGLTDLIVRRHQDGSTVSCHPDEPLNVAYQRMRLHDVSQMPVLDDDKVVGVLDEWDLLLSVHGEPENFRQTVAT
AMSSHVRTLSPQASLQDLLQVFNDGHIALIVDKGRYLGLITQADLLSFWRRQPRAA
>Mature_456_residues
MQDNSVLSLIGNTPLVRVSHLDTGPCELYLKLESHNPGGSIKDRIALTMIEAAERDGKLKPGGAIIEATAGNTGLGLALV
AAQKGYPLTLVVPDKMSREKILHLQALGAQVVLTRSDVGKGHPEYYQDLAARIASETPGAYYIDQFNNPANPLAHETTTG
PEIWRQMNGEVDAVVVGVGSSGTLTGLTRFFRAKAPATAFVLADPVGSVLADYVETGSFGQAGSWLIEGIGEDFIPPQTD
LSMVKRAFRVSDQESFDTAQQLLRSEGILAGSSSGALLAAALRYCREQTEPKRVVTLVCDSGNKYLSKMYNRDWLVDQGM
LRLPEVGGLTDLIVRRHQDGSTVSCHPDEPLNVAYQRMRLHDVSQMPVLDDDKVVGVLDEWDLLLSVHGEPENFRQTVAT
AMSSHVRTLSPQASLQDLLQVFNDGHIALIVDKGRYLGLITQADLLSFWRRQPRAA

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=473, Percent_Identity=35.0951374207188, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI295821200, Length=473, Percent_Identity=35.0951374207188, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI4557415, Length=473, Percent_Identity=35.0951374207188, Blast_Score=272, Evalue=4e-73,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=40.6666666666667, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788754, Length=321, Percent_Identity=41.1214953271028, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=39.7553516819572, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17534315, Length=330, Percent_Identity=41.8181818181818, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17562970, Length=310, Percent_Identity=38.7096774193548, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI32566674, Length=301, Percent_Identity=39.5348837209302, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=40.1315789473684, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI115535073, Length=303, Percent_Identity=42.2442244224422, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17561720, Length=302, Percent_Identity=40.0662251655629, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=37.6106194690265, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71996324, Length=343, Percent_Identity=26.530612244898, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17539800, Length=358, Percent_Identity=24.8603351955307, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17539640, Length=361, Percent_Identity=26.0387811634349, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=497, Percent_Identity=32.1931589537223, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321449, Length=338, Percent_Identity=35.7988165680473, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24643623, Length=467, Percent_Identity=32.9764453961456, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI20129101, Length=467, Percent_Identity=32.9764453961456, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 49582; Mature: 49582

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNSVLSLIGNTPLVRVSHLDTGPCELYLKLESHNPGGSIKDRIALTMIEAAERDGKLK
CCCCCEEEEECCCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
PGGAIIEATAGNTGLGLALVAAQKGYPLTLVVPDKMSREKILHLQALGAQVVLTRSDVGK
CCCEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
GHPEYYQDLAARIASETPGAYYIDQFNNPANPLAHETTTGPEIWRQMNGEVDAVVVGVGS
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECC
SGTLTGLTRFFRAKAPATAFVLADPVGSVLADYVETGSFGQAGSWLIEGIGEDFIPPQTD
CCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCH
LSMVKRAFRVSDQESFDTAQQLLRSEGILAGSSSGALLAAALRYCREQTEPKRVVTLVCD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
SGNKYLSKMYNRDWLVDQGMLRLPEVGGLTDLIVRRHQDGSTVSCHPDEPLNVAYQRMRL
CCCHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
HDVSQMPVLDDDKVVGVLDEWDLLLSVHGEPENFRQTVATAMSSHVRTLSPQASLQDLLQ
HHHCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VFNDGHIALIVDKGRYLGLITQADLLSFWRRQPRAA
HHCCCCEEEEEECCCEEEEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQDNSVLSLIGNTPLVRVSHLDTGPCELYLKLESHNPGGSIKDRIALTMIEAAERDGKLK
CCCCCEEEEECCCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
PGGAIIEATAGNTGLGLALVAAQKGYPLTLVVPDKMSREKILHLQALGAQVVLTRSDVGK
CCCEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
GHPEYYQDLAARIASETPGAYYIDQFNNPANPLAHETTTGPEIWRQMNGEVDAVVVGVGS
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECC
SGTLTGLTRFFRAKAPATAFVLADPVGSVLADYVETGSFGQAGSWLIEGIGEDFIPPQTD
CCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCH
LSMVKRAFRVSDQESFDTAQQLLRSEGILAGSSSGALLAAALRYCREQTEPKRVVTLVCD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
SGNKYLSKMYNRDWLVDQGMLRLPEVGGLTDLIVRRHQDGSTVSCHPDEPLNVAYQRMRL
CCCHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHH
HDVSQMPVLDDDKVVGVLDEWDLLLSVHGEPENFRQTVATAMSSHVRTLSPQASLQDLLQ
HHHCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VFNDGHIALIVDKGRYLGLITQADLLSFWRRQPRAA
HHCCCCEEEEEECCCEEEEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]