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The map label for this gene is dcuB [H]

Identifier: 34498825

GI number: 34498825

Start: 3665911

End: 3667239

Strand: Reverse

Name: dcuB [H]

Synonym: CV_3370

Alternate gene names: 34498825

Gene position: 3667239-3665911 (Counterclockwise)

Preceding gene: 34498826

Following gene: 34498824

Centisome position: 77.19

GC content: 64.94

Gene sequence:

>1329_bases
ATGTGGACTTTCTGGATTGAATTCGCGTTGCTGCTGACCGCCATCCTGATCGGGATACGGCGCGGCGGCGTGGCGCTGGG
GATGATAGGCGGCTTGGGCGTCGCGGTGATGGCCTTCGTGTTCCGGGTGGCGCCGGCCGAGCCGCCGATCACGGTGATGT
TGATCATCCTGGCGGTGGTGACCGCGTCGGCCACGCTGCAGGTGGCGGGCGGGCTGGACTTCCTGGTGCAGCAGGCGGAG
CGGCTGATGCGCAGGCATCCGCAGCAGATCACCTTCCTCGCGCCCTTGTCCACCTTTCTGCTCACCATGTGCGTCGGCAC
CGGCCACGCCGTGTATTCGCTGCTGCCGGTGATCGCCGACGTGTCGCTGAAAACCAAGATCCGCCCGGAGCGGCCGATGG
CGATGGCCAGCGTGGCCTCGCAGATGGGCATCACCGCCAGCCCGGCGGCGGCGGCGGTGACCACGCTGCTGGCGCTGACC
GCCAAGAGCGGGCAGCCGCTGAGCTTGTGGCAGATTCTGATCGTGACCATTCCGGCCGGTTTGGTCGGCGTGCTGGCCGC
CGCCTTCTGGAGCCTGAGGCGCGGCGCCGAACTGGATCAGGATCCGGAATACCAGGCGCGGCTGCTGGACCCGGATTTCC
GCGCGTCGATAGAAAAATCGGTGACCACGCTGGACAAGACGCTGCCCGCCCACGCCAAGCCGGCGGTGGTCCTGTTCTTC
TCCGGCATTTTGCTGGTGGTGCTGCTGGCGCTGTTCCCGCACTGGCTGCCGCTGGACGGCAAGGGCAAGCCGGTGCCGAT
GACGACGGCGGTGCAGTTCGTGATGCTGGCCTTCGGCGCCTTCATCCTGTTCCTGTCCAAGGCCAAGGCCGCCGTGATCG
CCCGCTCCGAAGTATTTACCGCCGGCATGATCGCCGTGGTGTCCATCTTCGGCATCGCCTGGATGAGCGACACCTTCGTC
AAGGCCAACGAGGCCTTCCTGGTCGACAACATCAAGGTGATGGTGGCCTACGCGCCGTGGACCTTCGCGCTGGCGATGTT
CGCGGTGTCGGCCTTCGTCAAGAGCCAGGCCGCCACGCTGACCATCATGGTGCCGTTCGGCCTGGCGCTGGGGCTGACCC
CGCACCAGTTGCTGGCCATCATGCCGTCCTGTTACGCCTATTTCTTTTTCGCCTTTTATCCCAGCGACCTGGCGGCCATC
AATATGGACCGCAGCGGCACCACCCGCATCGGCAAATATCTGCTCAACCACAGTTTCATGATGCCGGGCCTGATCGGGGT
GGGCGTGTCCACGTTGGCGGCCTACGCATTGACACATTTTTATTTCTGA

Upstream 100 bases:

>100_bases
ATCCGCATCCGCGCATGTCCTATAAACGGTCGCAATAGCAAGCGGCTGCGTTTCCGGCCCGGCTCATCCGGAGCCGGCCG
GGCGGCCACGGAGAGCAGGC

Downstream 100 bases:

>100_bases
CGAGGGGGAGTCCGTGAAGACCTGCCAAGCAGACGTAGTGATTGTCGGCGCCGGCGGCGCCGGCCTGAGGGCGGCCATCG
CCGCCGCGCAGTTCGATCCC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MWTFWIEFALLLTAILIGIRRGGVALGMIGGLGVAVMAFVFRVAPAEPPITVMLIILAVVTASATLQVAGGLDFLVQQAE
RLMRRHPQQITFLAPLSTFLLTMCVGTGHAVYSLLPVIADVSLKTKIRPERPMAMASVASQMGITASPAAAAVTTLLALT
AKSGQPLSLWQILIVTIPAGLVGVLAAAFWSLRRGAELDQDPEYQARLLDPDFRASIEKSVTTLDKTLPAHAKPAVVLFF
SGILLVVLLALFPHWLPLDGKGKPVPMTTAVQFVMLAFGAFILFLSKAKAAVIARSEVFTAGMIAVVSIFGIAWMSDTFV
KANEAFLVDNIKVMVAYAPWTFALAMFAVSAFVKSQAATLTIMVPFGLALGLTPHQLLAIMPSCYAYFFFAFYPSDLAAI
NMDRSGTTRIGKYLLNHSFMMPGLIGVGVSTLAAYALTHFYF

Sequences:

>Translated_442_residues
MWTFWIEFALLLTAILIGIRRGGVALGMIGGLGVAVMAFVFRVAPAEPPITVMLIILAVVTASATLQVAGGLDFLVQQAE
RLMRRHPQQITFLAPLSTFLLTMCVGTGHAVYSLLPVIADVSLKTKIRPERPMAMASVASQMGITASPAAAAVTTLLALT
AKSGQPLSLWQILIVTIPAGLVGVLAAAFWSLRRGAELDQDPEYQARLLDPDFRASIEKSVTTLDKTLPAHAKPAVVLFF
SGILLVVLLALFPHWLPLDGKGKPVPMTTAVQFVMLAFGAFILFLSKAKAAVIARSEVFTAGMIAVVSIFGIAWMSDTFV
KANEAFLVDNIKVMVAYAPWTFALAMFAVSAFVKSQAATLTIMVPFGLALGLTPHQLLAIMPSCYAYFFFAFYPSDLAAI
NMDRSGTTRIGKYLLNHSFMMPGLIGVGVSTLAAYALTHFYF
>Mature_442_residues
MWTFWIEFALLLTAILIGIRRGGVALGMIGGLGVAVMAFVFRVAPAEPPITVMLIILAVVTASATLQVAGGLDFLVQQAE
RLMRRHPQQITFLAPLSTFLLTMCVGTGHAVYSLLPVIADVSLKTKIRPERPMAMASVASQMGITASPAAAAVTTLLALT
AKSGQPLSLWQILIVTIPAGLVGVLAAAFWSLRRGAELDQDPEYQARLLDPDFRASIEKSVTTLDKTLPAHAKPAVVLFF
SGILLVVLLALFPHWLPLDGKGKPVPMTTAVQFVMLAFGAFILFLSKAKAAVIARSEVFTAGMIAVVSIFGIAWMSDTFV
KANEAFLVDNIKVMVAYAPWTFALAMFAVSAFVKSQAATLTIMVPFGLALGLTPHQLLAIMPSCYAYFFFAFYPSDLAAI
NMDRSGTTRIGKYLLNHSFMMPGLIGVGVSTLAAYALTHFYF

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=407, Percent_Identity=44.2260442260442, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790580, Length=438, Percent_Identity=37.6712328767123, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47468; Mature: 47468

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTFWIEFALLLTAILIGIRRGGVALGMIGGLGVAVMAFVFRVAPAEPPITVMLIILAVV
CHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TASATLQVAGGLDFLVQQAERLMRRHPQQITFLAPLSTFLLTMCVGTGHAVYSLLPVIAD
HCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHC
VSLKTKIRPERPMAMASVASQMGITASPAAAAVTTLLALTAKSGQPLSLWQILIVTIPAG
CCHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LVGVLAAAFWSLRRGAELDQDPEYQARLLDPDFRASIEKSVTTLDKTLPAHAKPAVVLFF
HHHHHHHHHHHHHCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SGILLVVLLALFPHWLPLDGKGKPVPMTTAVQFVMLAFGAFILFLSKAKAAVIARSEVFT
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGMIAVVSIFGIAWMSDTFVKANEAFLVDNIKVMVAYAPWTFALAMFAVSAFVKSQAATL
HHHHHHHHHHHHHHHCCHHHCCCCEEEEECEEEEEEECCHHHHHHHHHHHHHHHCCCCEE
TIMVPFGLALGLTPHQLLAIMPSCYAYFFFAFYPSDLAAINMDRSGTTRIGKYLLNHSFM
EEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCHH
MPGLIGVGVSTLAAYALTHFYF
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWTFWIEFALLLTAILIGIRRGGVALGMIGGLGVAVMAFVFRVAPAEPPITVMLIILAVV
CHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TASATLQVAGGLDFLVQQAERLMRRHPQQITFLAPLSTFLLTMCVGTGHAVYSLLPVIAD
HCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHC
VSLKTKIRPERPMAMASVASQMGITASPAAAAVTTLLALTAKSGQPLSLWQILIVTIPAG
CCHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LVGVLAAAFWSLRRGAELDQDPEYQARLLDPDFRASIEKSVTTLDKTLPAHAKPAVVLFF
HHHHHHHHHHHHHCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SGILLVVLLALFPHWLPLDGKGKPVPMTTAVQFVMLAFGAFILFLSKAKAAVIARSEVFT
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGMIAVVSIFGIAWMSDTFVKANEAFLVDNIKVMVAYAPWTFALAMFAVSAFVKSQAATL
HHHHHHHHHHHHHHHCCHHHCCCCEEEEECEEEEEEECCHHHHHHHHHHHHHHHCCCCEE
TIMVPFGLALGLTPHQLLAIMPSCYAYFFFAFYPSDLAAINMDRSGTTRIGKYLLNHSFM
EEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCHH
MPGLIGVGVSTLAAYALTHFYF
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]